Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor

Autores
Kamerbeek, Constanza B.; Mateos, Melina V.; Vallés, Ana Sofía; Pediconi, María F.; Barrantes, Francisco José; Borroni, María Virginia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Kamerbeek, Constanza B. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Mateos, Melina V. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Vallés, Ana Sofía. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pediconi, María F. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Barrantes, Francisco José. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Barrantes, Francisco José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Borroni, María Virginia. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Abstract: Diacylglycerol (DAG), a second messenger involved in different cell signaling cascades, activates protein kinase C (PKC) and D (PKD), among other kinases. The present work analyzes the effects resulting from the alteration of DAG levels on neuronal and muscle nicotinic acetylcholine receptor (AChR) distribution. We employ CHO-K1/A5 cells, expressing adult muscle-type AChR in a stable manner, and hippocampal neurons, which endogenously express various subtypes of neuronal AChR. CHO-K1/A5 cells treated with dioctanoylglycerol (DOG) for different periods showed augmented AChR cell surface levels at short incubation times (30min-4h) whereas at longer times (18h) the AChR was shifted to intracellular compartments. Similarly, in cultured hippocampal neurons surface AChR levels increased as a result of DOG incubation for 4h. Inhibition of endogenous DAG catabolism produced changes in AChR distribution similar to those induced by DOG treatment. Specific enzyme inhibitors and Western blot assays revealed that DAGs exert their effect on AChR distribution through the modulation of the activity of classical PKC (cPKC), novel PKC (nPKC) and PKD activity.
Fuente
The International Journal of Biochemistry & Cell Biology. 2016, 74
Materia
DIGLICERIDOS
QUINASA
RECEPTOR NICOTINICO
ENZIMAS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/15214
Acceder
id RIUCA_ed8f397ead3c35099da3580faafbfd13
oai_identifier_str oai:ucacris:123456789/15214
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptorKamerbeek, Constanza B.Mateos, Melina V.Vallés, Ana SofíaPediconi, María F.Barrantes, Francisco JoséBorroni, María VirginiaDIGLICERIDOSQUINASARECEPTOR NICOTINICOENZIMASFil: Kamerbeek, Constanza B. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Mateos, Melina V. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Vallés, Ana Sofía. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Pediconi, María F. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Barrantes, Francisco José. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Barrantes, Francisco José. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Borroni, María Virginia. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaAbstract: Diacylglycerol (DAG), a second messenger involved in different cell signaling cascades, activates protein kinase C (PKC) and D (PKD), among other kinases. The present work analyzes the effects resulting from the alteration of DAG levels on neuronal and muscle nicotinic acetylcholine receptor (AChR) distribution. We employ CHO-K1/A5 cells, expressing adult muscle-type AChR in a stable manner, and hippocampal neurons, which endogenously express various subtypes of neuronal AChR. CHO-K1/A5 cells treated with dioctanoylglycerol (DOG) for different periods showed augmented AChR cell surface levels at short incubation times (30min-4h) whereas at longer times (18h) the AChR was shifted to intracellular compartments. Similarly, in cultured hippocampal neurons surface AChR levels increased as a result of DOG incubation for 4h. Inhibition of endogenous DAG catabolism produced changes in AChR distribution similar to those induced by DOG treatment. Specific enzyme inhibitors and Western blot assays revealed that DAGs exert their effect on AChR distribution through the modulation of the activity of classical PKC (cPKC), novel PKC (nPKC) and PKD activity.Elsevier2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/152141357-272510.1016/j.biocel.2016.02.01026898898Kamerbeek, C.B. Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor [en línea]. The International Journal of Biochemistry & Cell Biology. 2016, 74 doi:10.1016/j.biocel.2016.02.010 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/15214The International Journal of Biochemistry & Cell Biology. 2016, 74reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:58:53Zoai:ucacris:123456789/15214instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:58:54.021Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
title Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
spellingShingle Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
Kamerbeek, Constanza B.
DIGLICERIDOS
QUINASA
RECEPTOR NICOTINICO
ENZIMAS
title_short Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
title_full Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
title_fullStr Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
title_full_unstemmed Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
title_sort Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor
dc.creator.none.fl_str_mv Kamerbeek, Constanza B.
Mateos, Melina V.
Vallés, Ana Sofía
Pediconi, María F.
Barrantes, Francisco José
Borroni, María Virginia
author Kamerbeek, Constanza B.
author_facet Kamerbeek, Constanza B.
Mateos, Melina V.
Vallés, Ana Sofía
Pediconi, María F.
Barrantes, Francisco José
Borroni, María Virginia
author_role author
author2 Mateos, Melina V.
Vallés, Ana Sofía
Pediconi, María F.
Barrantes, Francisco José
Borroni, María Virginia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv DIGLICERIDOS
QUINASA
RECEPTOR NICOTINICO
ENZIMAS
topic DIGLICERIDOS
QUINASA
RECEPTOR NICOTINICO
ENZIMAS
dc.description.none.fl_txt_mv Fil: Kamerbeek, Constanza B. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Mateos, Melina V. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Vallés, Ana Sofía. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pediconi, María F. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Barrantes, Francisco José. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Barrantes, Francisco José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Borroni, María Virginia. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Abstract: Diacylglycerol (DAG), a second messenger involved in different cell signaling cascades, activates protein kinase C (PKC) and D (PKD), among other kinases. The present work analyzes the effects resulting from the alteration of DAG levels on neuronal and muscle nicotinic acetylcholine receptor (AChR) distribution. We employ CHO-K1/A5 cells, expressing adult muscle-type AChR in a stable manner, and hippocampal neurons, which endogenously express various subtypes of neuronal AChR. CHO-K1/A5 cells treated with dioctanoylglycerol (DOG) for different periods showed augmented AChR cell surface levels at short incubation times (30min-4h) whereas at longer times (18h) the AChR was shifted to intracellular compartments. Similarly, in cultured hippocampal neurons surface AChR levels increased as a result of DOG incubation for 4h. Inhibition of endogenous DAG catabolism produced changes in AChR distribution similar to those induced by DOG treatment. Specific enzyme inhibitors and Western blot assays revealed that DAGs exert their effect on AChR distribution through the modulation of the activity of classical PKC (cPKC), novel PKC (nPKC) and PKD activity.
description Fil: Kamerbeek, Constanza B. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/15214
1357-2725
10.1016/j.biocel.2016.02.010
26898898
Kamerbeek, C.B. Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor [en línea]. The International Journal of Biochemistry & Cell Biology. 2016, 74 doi:10.1016/j.biocel.2016.02.010 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/15214
url https://repositorio.uca.edu.ar/handle/123456789/15214
identifier_str_mv 1357-2725
10.1016/j.biocel.2016.02.010
26898898
Kamerbeek, C.B. Diacylglycerol levels modulate the cellular distribution of the nicotinic acetylcholine receptor [en línea]. The International Journal of Biochemistry & Cell Biology. 2016, 74 doi:10.1016/j.biocel.2016.02.010 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/15214
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv The International Journal of Biochemistry & Cell Biology. 2016, 74
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.22299

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