Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clock

Autores
Plano, Santiago Andrés; Alessandro, María Soledad; Trebucq, Laura Lucía; Endo, Shogo; Golombek, Diego A.; Chiesa, Juan José
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Plano, Santiago Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Plano, Santiago Andrés. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Alessandro, María Soledad. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Plano, Santiago Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Alessandro, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trebucq, Laura Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trebucq, Laura Lucía. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Endo, Shogo. Instituto Metropolitano de Gerontología de Tokio; Japón
Fil: Golombek, Diego A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Golombek, Diego A. Universidad Nacional de Quilmes. Laboratorio de Cronobiología; Argentina
Fil: Chiesa, Juan José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Chiesa, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Abstract: The mammalian circadian clock at the hypothalamic suprachiasmatic nuclei (SCN) entrains biological rhythms to the 24- h cyclic environment, by encoding light-dark transitions in SCN neurons. Light pulses induce phase shifts in the clock and in circadian rhythms; photic signaling for circadian phase advances involves a nitric oxide (NO)/cyclic guanosine monophosphate (cGMP)/cGMP-dependent protein kinase (PKG) pathway, increasing the expression of Period (Per) genes. Effectors downstream of PKG remain unknown. Here we investigate the role of G-substrate (GS), a PKG substrate, in the hamster SCN. GS and phosphorylated G-substrate (p-GS) were present in a subset of SCN cells. Moreover, GS phosphorylation (p- GS/GS ratio) increased in SCN homogenates after light pulses delivered at circadian time (CT) 18 and intraperitoneal treatment with sildenafil, an inhibitor of phosphodiesterase 5 (a cGMP-specific phosphodiesterase). On the other hand, intracerebroventricular treatment with the PKG inhibitor KT5823, reduced photic phosphorylation of GS to basal levels. Since p-GS could act as a protein phosphatase 2 A (PP2A) inhibitor, we demonstrated physical interaction between p-GS and PP2A in SCN homogenates, and also a light-pulse dependent decrease of PP2A activity. Intracerebroventricular treatment with okadaic acid, a PP2A inhibitor, increased the magnitude of light-induced phase advances of locomotor rhythms. We provide evidence on the physiological phosphorylation of GS as a new downstream effector in the NO/cGMP/PKG photic pathway in the hamster SCN, including its role as a PP2A inhibitor.
Fuente
American Society for Neurochemistry. 2021, 13(1-12)
Materia
NUCLEO SUPRAQUIASMATICO
PROTEíNA FOSFATASA 2A
SISTEMA NERVIOSO CENTRAL
LUZ
RITMO CIRCADIANO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/11524
Acceder
id RIUCA_bad0a5ed0c5d4957196e231b6a7bfe98
oai_identifier_str oai:ucacris:123456789/11524
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clockPlano, Santiago AndrésAlessandro, María SoledadTrebucq, Laura LucíaEndo, ShogoGolombek, Diego A.Chiesa, Juan JoséNUCLEO SUPRAQUIASMATICOPROTEíNA FOSFATASA 2ASISTEMA NERVIOSO CENTRALLUZRITMO CIRCADIANOFil: Plano, Santiago Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Plano, Santiago Andrés. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Alessandro, María Soledad. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaFil: Plano, Santiago Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaFil: Alessandro, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trebucq, Laura Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trebucq, Laura Lucía. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaFil: Endo, Shogo. Instituto Metropolitano de Gerontología de Tokio; JapónFil: Golombek, Diego A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Golombek, Diego A. Universidad Nacional de Quilmes. Laboratorio de Cronobiología; ArgentinaFil: Chiesa, Juan José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; ArgentinaFil: Chiesa, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAbstract: The mammalian circadian clock at the hypothalamic suprachiasmatic nuclei (SCN) entrains biological rhythms to the 24- h cyclic environment, by encoding light-dark transitions in SCN neurons. Light pulses induce phase shifts in the clock and in circadian rhythms; photic signaling for circadian phase advances involves a nitric oxide (NO)/cyclic guanosine monophosphate (cGMP)/cGMP-dependent protein kinase (PKG) pathway, increasing the expression of Period (Per) genes. Effectors downstream of PKG remain unknown. Here we investigate the role of G-substrate (GS), a PKG substrate, in the hamster SCN. GS and phosphorylated G-substrate (p-GS) were present in a subset of SCN cells. Moreover, GS phosphorylation (p- GS/GS ratio) increased in SCN homogenates after light pulses delivered at circadian time (CT) 18 and intraperitoneal treatment with sildenafil, an inhibitor of phosphodiesterase 5 (a cGMP-specific phosphodiesterase). On the other hand, intracerebroventricular treatment with the PKG inhibitor KT5823, reduced photic phosphorylation of GS to basal levels. Since p-GS could act as a protein phosphatase 2 A (PP2A) inhibitor, we demonstrated physical interaction between p-GS and PP2A in SCN homogenates, and also a light-pulse dependent decrease of PP2A activity. Intracerebroventricular treatment with okadaic acid, a PP2A inhibitor, increased the magnitude of light-induced phase advances of locomotor rhythms. We provide evidence on the physiological phosphorylation of GS as a new downstream effector in the NO/cGMP/PKG photic pathway in the hamster SCN, including its role as a PP2A inhibitor.SAGE Publishing2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/115241759-0914 (on line)10.1177/175909142098492033430619Plano, S. A., Alessandro, M. S., Trebucq, L. L., Endo, S., Golombek, D. A., Chiesa, J. J. Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clock [en línea]. American Society for Neurochemistry. 2021, 13(1-12). Doi: 10.1177/1759091420984920. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/11524American Society for Neurochemistry. 2021, 13(1-12)reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:57:48Zoai:ucacris:123456789/11524instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:57:49.113Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clock
title Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clock
spellingShingle Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clock
Plano, Santiago Andrés
NUCLEO SUPRAQUIASMATICO
PROTEíNA FOSFATASA 2A
SISTEMA NERVIOSO CENTRAL
LUZ
RITMO CIRCADIANO
title_short Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clock
title_full Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clock
title_fullStr Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clock
title_full_unstemmed Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clock
title_sort Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clock
dc.creator.none.fl_str_mv Plano, Santiago Andrés
Alessandro, María Soledad
Trebucq, Laura Lucía
Endo, Shogo
Golombek, Diego A.
Chiesa, Juan José
author Plano, Santiago Andrés
author_facet Plano, Santiago Andrés
Alessandro, María Soledad
Trebucq, Laura Lucía
Endo, Shogo
Golombek, Diego A.
Chiesa, Juan José
author_role author
author2 Alessandro, María Soledad
Trebucq, Laura Lucía
Endo, Shogo
Golombek, Diego A.
Chiesa, Juan José
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv NUCLEO SUPRAQUIASMATICO
PROTEíNA FOSFATASA 2A
SISTEMA NERVIOSO CENTRAL
LUZ
RITMO CIRCADIANO
topic NUCLEO SUPRAQUIASMATICO
PROTEíNA FOSFATASA 2A
SISTEMA NERVIOSO CENTRAL
LUZ
RITMO CIRCADIANO
dc.description.none.fl_txt_mv Fil: Plano, Santiago Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Plano, Santiago Andrés. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Alessandro, María Soledad. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Plano, Santiago Andrés. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Alessandro, María Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trebucq, Laura Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trebucq, Laura Lucía. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Endo, Shogo. Instituto Metropolitano de Gerontología de Tokio; Japón
Fil: Golombek, Diego A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Golombek, Diego A. Universidad Nacional de Quilmes. Laboratorio de Cronobiología; Argentina
Fil: Chiesa, Juan José. Universidad Nacional de Quilmes. Departamento de Ciencia y Tecnología. Laboratorio de Cronobiología; Argentina
Fil: Chiesa, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Abstract: The mammalian circadian clock at the hypothalamic suprachiasmatic nuclei (SCN) entrains biological rhythms to the 24- h cyclic environment, by encoding light-dark transitions in SCN neurons. Light pulses induce phase shifts in the clock and in circadian rhythms; photic signaling for circadian phase advances involves a nitric oxide (NO)/cyclic guanosine monophosphate (cGMP)/cGMP-dependent protein kinase (PKG) pathway, increasing the expression of Period (Per) genes. Effectors downstream of PKG remain unknown. Here we investigate the role of G-substrate (GS), a PKG substrate, in the hamster SCN. GS and phosphorylated G-substrate (p-GS) were present in a subset of SCN cells. Moreover, GS phosphorylation (p- GS/GS ratio) increased in SCN homogenates after light pulses delivered at circadian time (CT) 18 and intraperitoneal treatment with sildenafil, an inhibitor of phosphodiesterase 5 (a cGMP-specific phosphodiesterase). On the other hand, intracerebroventricular treatment with the PKG inhibitor KT5823, reduced photic phosphorylation of GS to basal levels. Since p-GS could act as a protein phosphatase 2 A (PP2A) inhibitor, we demonstrated physical interaction between p-GS and PP2A in SCN homogenates, and also a light-pulse dependent decrease of PP2A activity. Intracerebroventricular treatment with okadaic acid, a PP2A inhibitor, increased the magnitude of light-induced phase advances of locomotor rhythms. We provide evidence on the physiological phosphorylation of GS as a new downstream effector in the NO/cGMP/PKG photic pathway in the hamster SCN, including its role as a PP2A inhibitor.
description Fil: Plano, Santiago Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/11524
1759-0914 (on line)
10.1177/1759091420984920
33430619
Plano, S. A., Alessandro, M. S., Trebucq, L. L., Endo, S., Golombek, D. A., Chiesa, J. J. Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clock [en línea]. American Society for Neurochemistry. 2021, 13(1-12). Doi: 10.1177/1759091420984920. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/11524
url https://repositorio.uca.edu.ar/handle/123456789/11524
identifier_str_mv 1759-0914 (on line)
10.1177/1759091420984920
33430619
Plano, S. A., Alessandro, M. S., Trebucq, L. L., Endo, S., Golombek, D. A., Chiesa, J. J. Role of G-substrate in the NO/cGMP/PKG signal transduction pathway for photic entrainment of the hamster circadian clock [en línea]. American Society for Neurochemistry. 2021, 13(1-12). Doi: 10.1177/1759091420984920. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/11524
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv SAGE Publishing
publisher.none.fl_str_mv SAGE Publishing
dc.source.none.fl_str_mv American Society for Neurochemistry. 2021, 13(1-12)
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.070432

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