Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity

Autores
Campagna, Anabella Andrea; Fabra, Mariana Carolina; Nikoloff, Noelia; Carranza Martin, Ana Cristina
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Campagna, Anabella Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Argentina
Fil: Campagna, Anabella Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Argentina
Fil: Campagna, Anabella Andrea. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción; Argentina
Fil: Fabra, Mariana Carolina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Argentina
Fil: Fabra, Mariana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Argentina
Fil: Nikoloff, Noelia. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción; Argentina
Fil: Carranza Martin, Ana Cristina. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción; Argentina
Fluazuron is a veterinary antiparasitic compound widely used as pour-on in cattle. However, little is known about its potential reproductive toxicity. This study aimed to evaluate the effects of fluazuron on sperm quality, bovine oviduct epithelial cells (BOECs), and bovine in vitro fertilization (IVF). Frozen-thawed sperm were exposed to fluazuron (50 µg/mL) for 2 h and evaluated for motility, mitochondrial activity, and membrane integrity, while BOECs were cultured with fluazuron for 24 h and assessed using MTT and comet assays. IVF was performed with gametes exposed to fluazuron for 18 h to evaluate pronuclear formation, and 24 h to study embryo development. Short-term fluazuron did not significantly affect sperm motility, viability, or membrane function. However, it decreased viability and induced DNA damage in BOECs after 24 h of treatment. Following fluazuron exposure during IVF, no differences were observed between the fluazuron-treated group and the control group in terms of pronuclear formation. However, a significantly higher incidence of abnormally condensed chromatin within the pronuclei was detected in the fluazuron-treated group. Furthermore, embryonic development decreased in both blastocyst and hatching rates in fluazuron treatment. These findings suggest that fluazuron may disrupt early reproductive events through multiple mechanisms, including BOEC dysfunction and chromatin damage. Our results highlight the importance of evaluating reproductive risks associated with antiparasitic drug exposure in livestock
Fuente
Reproductive Biology. 25, 2025
Materia
FERTILIZACION IN VITRO
ANTIPARASITARIOS
TOXICIDAD
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/20653
Acceder
id RIUCA_91c0c0379ca6885c28fd6c693128b495
oai_identifier_str oai:ucacris:123456789/20653
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrityCampagna, Anabella AndreaFabra, Mariana CarolinaNikoloff, NoeliaCarranza Martin, Ana CristinaFERTILIZACION IN VITROANTIPARASITARIOSTOXICIDADFil: Campagna, Anabella Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. ArgentinaFil: Campagna, Anabella Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. ArgentinaFil: Campagna, Anabella Andrea. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción; ArgentinaFil: Fabra, Mariana Carolina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. ArgentinaFil: Fabra, Mariana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. ArgentinaFil: Nikoloff, Noelia. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción; ArgentinaFil: Carranza Martin, Ana Cristina. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción; ArgentinaFluazuron is a veterinary antiparasitic compound widely used as pour-on in cattle. However, little is known about its potential reproductive toxicity. This study aimed to evaluate the effects of fluazuron on sperm quality, bovine oviduct epithelial cells (BOECs), and bovine in vitro fertilization (IVF). Frozen-thawed sperm were exposed to fluazuron (50 µg/mL) for 2 h and evaluated for motility, mitochondrial activity, and membrane integrity, while BOECs were cultured with fluazuron for 24 h and assessed using MTT and comet assays. IVF was performed with gametes exposed to fluazuron for 18 h to evaluate pronuclear formation, and 24 h to study embryo development. Short-term fluazuron did not significantly affect sperm motility, viability, or membrane function. However, it decreased viability and induced DNA damage in BOECs after 24 h of treatment. Following fluazuron exposure during IVF, no differences were observed between the fluazuron-treated group and the control group in terms of pronuclear formation. However, a significantly higher incidence of abnormally condensed chromatin within the pronuclei was detected in the fluazuron-treated group. Furthermore, embryonic development decreased in both blastocyst and hatching rates in fluazuron treatment. These findings suggest that fluazuron may disrupt early reproductive events through multiple mechanisms, including BOEC dysfunction and chromatin damage. Our results highlight the importance of evaluating reproductive risks associated with antiparasitic drug exposure in livestockElsevier2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/206531642-431X10.1016/j.repbio.2025.101087Reproductive Biology. 25, 2025reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-12-18T09:51:07Zoai:ucacris:123456789/20653instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-12-18 09:51:07.231Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity
title Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity
spellingShingle Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity
Campagna, Anabella Andrea
FERTILIZACION IN VITRO
ANTIPARASITARIOS
TOXICIDAD
title_short Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity
title_full Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity
title_fullStr Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity
title_full_unstemmed Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity
title_sort Toxicological effects of fluazuron on bovine reproductive cells: Implications for fertilization, sperm quality, and oviductal integrity
dc.creator.none.fl_str_mv Campagna, Anabella Andrea
Fabra, Mariana Carolina
Nikoloff, Noelia
Carranza Martin, Ana Cristina
author Campagna, Anabella Andrea
author_facet Campagna, Anabella Andrea
Fabra, Mariana Carolina
Nikoloff, Noelia
Carranza Martin, Ana Cristina
author_role author
author2 Fabra, Mariana Carolina
Nikoloff, Noelia
Carranza Martin, Ana Cristina
author2_role author
author
author
dc.subject.none.fl_str_mv FERTILIZACION IN VITRO
ANTIPARASITARIOS
TOXICIDAD
topic FERTILIZACION IN VITRO
ANTIPARASITARIOS
TOXICIDAD
dc.description.none.fl_txt_mv Fil: Campagna, Anabella Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Argentina
Fil: Campagna, Anabella Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Argentina
Fil: Campagna, Anabella Andrea. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción; Argentina
Fil: Fabra, Mariana Carolina. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Argentina
Fil: Fabra, Mariana Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Argentina
Fil: Nikoloff, Noelia. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción; Argentina
Fil: Carranza Martin, Ana Cristina. Pontificia Universidad Católica Argentina. Facultad de Ingeniería y Ciencias Agrarias. Laboratorio de Reproducción; Argentina
Fluazuron is a veterinary antiparasitic compound widely used as pour-on in cattle. However, little is known about its potential reproductive toxicity. This study aimed to evaluate the effects of fluazuron on sperm quality, bovine oviduct epithelial cells (BOECs), and bovine in vitro fertilization (IVF). Frozen-thawed sperm were exposed to fluazuron (50 µg/mL) for 2 h and evaluated for motility, mitochondrial activity, and membrane integrity, while BOECs were cultured with fluazuron for 24 h and assessed using MTT and comet assays. IVF was performed with gametes exposed to fluazuron for 18 h to evaluate pronuclear formation, and 24 h to study embryo development. Short-term fluazuron did not significantly affect sperm motility, viability, or membrane function. However, it decreased viability and induced DNA damage in BOECs after 24 h of treatment. Following fluazuron exposure during IVF, no differences were observed between the fluazuron-treated group and the control group in terms of pronuclear formation. However, a significantly higher incidence of abnormally condensed chromatin within the pronuclei was detected in the fluazuron-treated group. Furthermore, embryonic development decreased in both blastocyst and hatching rates in fluazuron treatment. These findings suggest that fluazuron may disrupt early reproductive events through multiple mechanisms, including BOEC dysfunction and chromatin damage. Our results highlight the importance of evaluating reproductive risks associated with antiparasitic drug exposure in livestock
description Fil: Campagna, Anabella Andrea. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Argentina
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/20653
1642-431X
10.1016/j.repbio.2025.101087
url https://repositorio.uca.edu.ar/handle/123456789/20653
identifier_str_mv 1642-431X
10.1016/j.repbio.2025.101087
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Reproductive Biology. 25, 2025
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 12.952241

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