Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability

Autores
Mosqueira, Alejo; Camino, Pablo A.; Barrantes, Francisco José
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Mosqueira, Alejo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentina
Fil: Mosqueira, Alejo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Camino, Pablo A. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentina
Fil: Camino, Pablo A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barrantes, Francisco J. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentina
Fil: Barrantes, Francisco J. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Abstract: Translational motion of neurotransmitter receptors is key for determining receptor number at the synapse and hence, synaptic efficacy. We combine live-cell STORM superresolution microscopy of nicotinic acetylcholine receptor (nAChR) with single-particle tracking, mean-squared displacement (MSD), turning angle, ergodicity, and clustering analyses to characterize the lateral motion of individual molecules and their collective behaviour. nAChR diffusion is highly heterogeneous: subdiffusive, Brownian and, less frequently, superdiffusive. At the single-track level, free walks are transiently interrupted by ms-long confinement sojourns occurring in nanodomains of ~36 nm radius. Cholesterol modulates the time and the area spent in confinement. Turning angle analysis reveals anticorrelated steps with time-lag dependence, in good agreement with the permeable fence model. At the ensemble level, nanocluster assembly occurs in second-long bursts separated by periods of cluster disassembly. Thus, millisecond-long confinement sojourns and second-long reversible nanoclustering with similar cholesterol sensitivities affect all trajectories; the proportion of the two regimes determines the resulting macroscopic motional mode and breadth of heterogeneity in the ensemble population.
Fuente
Scientific Reports. 2018, 8
Materia
COLESTEROL
MOLECULAS
LIPIDOS
NEUROTRANSMISORES
CEREBRO
TRANSMISIÓN SINAPTICA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/1464
Acceder
id RIUCA_0e9980c52c1bd04b332cf5fdbe87d39b
oai_identifier_str oai:ucacris:123456789/1464
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stabilityMosqueira, AlejoCamino, Pablo A.Barrantes, Francisco JoséCOLESTEROLMOLECULASLIPIDOSNEUROTRANSMISORESCEREBROTRANSMISIÓN SINAPTICAFil: Mosqueira, Alejo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; ArgentinaFil: Mosqueira, Alejo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Camino, Pablo A. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; ArgentinaFil: Camino, Pablo A. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barrantes, Francisco J. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; ArgentinaFil: Barrantes, Francisco J. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAbstract: Translational motion of neurotransmitter receptors is key for determining receptor number at the synapse and hence, synaptic efficacy. We combine live-cell STORM superresolution microscopy of nicotinic acetylcholine receptor (nAChR) with single-particle tracking, mean-squared displacement (MSD), turning angle, ergodicity, and clustering analyses to characterize the lateral motion of individual molecules and their collective behaviour. nAChR diffusion is highly heterogeneous: subdiffusive, Brownian and, less frequently, superdiffusive. At the single-track level, free walks are transiently interrupted by ms-long confinement sojourns occurring in nanodomains of ~36 nm radius. Cholesterol modulates the time and the area spent in confinement. Turning angle analysis reveals anticorrelated steps with time-lag dependence, in good agreement with the permeable fence model. At the ensemble level, nanocluster assembly occurs in second-long bursts separated by periods of cluster disassembly. Thus, millisecond-long confinement sojourns and second-long reversible nanoclustering with similar cholesterol sensitivities affect all trajectories; the proportion of the two regimes determines the resulting macroscopic motional mode and breadth of heterogeneity in the ensemble population.Nature Research2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/14642045-2322 (online)10.1038/s41598-018-30384-yMosqueira, A., Camino, P. A., Barranjes, F. J. Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability [en línea]. Scientific Reports. 2018, 8. doi:10.1038/s41598-018-30384-y. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1464Scientific Reports. 2018, 8reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaengenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:55:17Zoai:ucacris:123456789/1464instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:55:17.946Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability
title Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability
spellingShingle Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability
Mosqueira, Alejo
COLESTEROL
MOLECULAS
LIPIDOS
NEUROTRANSMISORES
CEREBRO
TRANSMISIÓN SINAPTICA
title_short Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability
title_full Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability
title_fullStr Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability
title_full_unstemmed Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability
title_sort Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability
dc.creator.none.fl_str_mv Mosqueira, Alejo
Camino, Pablo A.
Barrantes, Francisco José
author Mosqueira, Alejo
author_facet Mosqueira, Alejo
Camino, Pablo A.
Barrantes, Francisco José
author_role author
author2 Camino, Pablo A.
Barrantes, Francisco José
author2_role author
author
dc.subject.none.fl_str_mv COLESTEROL
MOLECULAS
LIPIDOS
NEUROTRANSMISORES
CEREBRO
TRANSMISIÓN SINAPTICA
topic COLESTEROL
MOLECULAS
LIPIDOS
NEUROTRANSMISORES
CEREBRO
TRANSMISIÓN SINAPTICA
dc.description.none.fl_txt_mv Fil: Mosqueira, Alejo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentina
Fil: Mosqueira, Alejo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Camino, Pablo A. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentina
Fil: Camino, Pablo A. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barrantes, Francisco J. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentina
Fil: Barrantes, Francisco J. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Abstract: Translational motion of neurotransmitter receptors is key for determining receptor number at the synapse and hence, synaptic efficacy. We combine live-cell STORM superresolution microscopy of nicotinic acetylcholine receptor (nAChR) with single-particle tracking, mean-squared displacement (MSD), turning angle, ergodicity, and clustering analyses to characterize the lateral motion of individual molecules and their collective behaviour. nAChR diffusion is highly heterogeneous: subdiffusive, Brownian and, less frequently, superdiffusive. At the single-track level, free walks are transiently interrupted by ms-long confinement sojourns occurring in nanodomains of ~36 nm radius. Cholesterol modulates the time and the area spent in confinement. Turning angle analysis reveals anticorrelated steps with time-lag dependence, in good agreement with the permeable fence model. At the ensemble level, nanocluster assembly occurs in second-long bursts separated by periods of cluster disassembly. Thus, millisecond-long confinement sojourns and second-long reversible nanoclustering with similar cholesterol sensitivities affect all trajectories; the proportion of the two regimes determines the resulting macroscopic motional mode and breadth of heterogeneity in the ensemble population.
description Fil: Mosqueira, Alejo. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas. Laboratorio de Neurobiología Molecular; Argentina
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/1464
2045-2322 (online)
10.1038/s41598-018-30384-y
Mosqueira, A., Camino, P. A., Barranjes, F. J. Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability [en línea]. Scientific Reports. 2018, 8. doi:10.1038/s41598-018-30384-y. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1464
url https://repositorio.uca.edu.ar/handle/123456789/1464
identifier_str_mv 2045-2322 (online)
10.1038/s41598-018-30384-y
Mosqueira, A., Camino, P. A., Barranjes, F. J. Cholesterol modulates acetylcholine receptor diffusion by tuning confinement sojourns and nanocluster stability [en línea]. Scientific Reports. 2018, 8. doi:10.1038/s41598-018-30384-y. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1464
dc.language.none.fl_str_mv eng
eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv Scientific Reports. 2018, 8
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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score 13.001348

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