Prevalence, serotypes and virulence genes of streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation

Autores
Bobadilla, Fernando Javier; Novosak, Marina Gisel; Cortese, Iliana Julieta; Delgado, Osvaldo Daniel; Laczeski, Margarita Ester
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Bobadilla, Fernando Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Bobadilla, Fernando Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina.
Fil: Novosak, Marina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Novosak, Marina Gisel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina.
Fil: Novosak, Marina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología de Misiones; Argentina.
Fil: Novosak, Marina Gisel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología de Misiones; Argentina.
Fil: Cortese, Iliana Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología de Misiones; Argentina.
Fil: Cortese, Iliana Julieta. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología de Misiones; Argentina.
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina.
Fil: Delgado, Osvaldo Daniel. Universidad Nacional de Catamarca. Planta Piloto de Procesos Industriales Microbiológicos; Argentina.
Fil: Laczeski, Margarita Ester. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Laczeski, Margarita Ester. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina.
Fil: Laczeski, Margarita Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología de Misiones; Argentina.
Fil: Laczeski, Margarita Ester. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología de Misiones; Argentina.
Background: In pregnant women Streptococcus agalactiae (GBS) can be transmitted to newborn causing severe infections. It is classified into 10 serotypes (Ia, Ib, II-IX). The severity of neonatal disease is determined by the capsular serotype and virulence factors such as the polysaccharide capsule, encoded by the cps gene, protein C, which includes the Cα surface proteins (bca gene), Rib (rib gene) and Cβ (bac gene); the proteins Lmb (lmb gene), FbsB (fbsB gene), FbsA (fbsA gene), the cyl operon encoding a β-hemolysin (hylB gene), the CAMP factor (cfb gene) and the C5a peptidase (scpB gene). The aim of this work was to determine the degree of GBS colonization in pregnant women, the serotypes distribution and to investigate virulence-associated genes. Methods: We worked with 3480 samples of vagino-rectal swabs of women with 35–37 weeks of gestation. The identification of the strains was carried out using conventional biochemical tests and group confirmatory serology using a commercial latex particle agglutination kit. Two hundred GBS strains were selected. Their serotype was determined by agglutination tests. The monoplex PCR technique was used to investigate nine virulence-associated genes (cps, bca, rib, bac, lmb, fbsB, fbsA, hylB and scpB). Results: The maternal colonization was 9.09%. The serotypes found were: Ia (33.50%), III (19.00%), Ib (15.50%), II (14.00%), V (7.00%) and IX (5.50%). 5.50% of strains were found to be non-serotypeable (NT). The nine virulence genes investigated were detected simultaneously in 36.50% of the strains. The genes that were most frequently detected were scpB (100.00%), fbsA (100.00%), fbsB (100.00%), cylB (95.00%), lmb (94.00%) and bca (87.50%). We found associations between serotype and genes bac (p = 0.003), cylB (p = 0.02), rib (p = 0.01) and lmb (p < 0.001). Conclusions: The frequency of vaginal-rectal colonization, serotypes distribution and associated virulence genes, varies widely among geographical areas. Therefore, epidemiological surveillance is necessary to provide data to guide decision-making and planning of prevention and control strategies.
Materia
GBS
Colonization
Serotypes
Virulence genes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 4.0 Internacional
Repositorio
Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
Institución
Universidad Nacional de Misiones
OAI Identificador
oai:rid.unam.edu.ar:20.500.12219/5099
Acceder
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network_acronym_str RIDUNaM
repository_id_str
network_name_str Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)
spelling Prevalence, serotypes and virulence genes of streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestationBobadilla, Fernando JavierNovosak, Marina GiselCortese, Iliana JulietaDelgado, Osvaldo DanielLaczeski, Margarita EsterGBSColonizationSerotypesVirulence genesFil: Bobadilla, Fernando Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Bobadilla, Fernando Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina.Fil: Novosak, Marina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Novosak, Marina Gisel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina.Fil: Novosak, Marina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología de Misiones; Argentina.Fil: Novosak, Marina Gisel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología de Misiones; Argentina.Fil: Cortese, Iliana Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología de Misiones; Argentina.Fil: Cortese, Iliana Julieta. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología de Misiones; Argentina.Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina.Fil: Delgado, Osvaldo Daniel. Universidad Nacional de Catamarca. Planta Piloto de Procesos Industriales Microbiológicos; Argentina.Fil: Laczeski, Margarita Ester. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Laczeski, Margarita Ester. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina.Fil: Laczeski, Margarita Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología de Misiones; Argentina.Fil: Laczeski, Margarita Ester. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología de Misiones; Argentina.Background: In pregnant women Streptococcus agalactiae (GBS) can be transmitted to newborn causing severe infections. It is classified into 10 serotypes (Ia, Ib, II-IX). The severity of neonatal disease is determined by the capsular serotype and virulence factors such as the polysaccharide capsule, encoded by the cps gene, protein C, which includes the Cα surface proteins (bca gene), Rib (rib gene) and Cβ (bac gene); the proteins Lmb (lmb gene), FbsB (fbsB gene), FbsA (fbsA gene), the cyl operon encoding a β-hemolysin (hylB gene), the CAMP factor (cfb gene) and the C5a peptidase (scpB gene). The aim of this work was to determine the degree of GBS colonization in pregnant women, the serotypes distribution and to investigate virulence-associated genes. Methods: We worked with 3480 samples of vagino-rectal swabs of women with 35–37 weeks of gestation. The identification of the strains was carried out using conventional biochemical tests and group confirmatory serology using a commercial latex particle agglutination kit. Two hundred GBS strains were selected. Their serotype was determined by agglutination tests. The monoplex PCR technique was used to investigate nine virulence-associated genes (cps, bca, rib, bac, lmb, fbsB, fbsA, hylB and scpB). Results: The maternal colonization was 9.09%. The serotypes found were: Ia (33.50%), III (19.00%), Ib (15.50%), II (14.00%), V (7.00%) and IX (5.50%). 5.50% of strains were found to be non-serotypeable (NT). The nine virulence genes investigated were detected simultaneously in 36.50% of the strains. The genes that were most frequently detected were scpB (100.00%), fbsA (100.00%), fbsB (100.00%), cylB (95.00%), lmb (94.00%) and bca (87.50%). We found associations between serotype and genes bac (p = 0.003), cylB (p = 0.02), rib (p = 0.01) and lmb (p < 0.001). Conclusions: The frequency of vaginal-rectal colonization, serotypes distribution and associated virulence genes, varies widely among geographical areas. Therefore, epidemiological surveillance is necessary to provide data to guide decision-making and planning of prevention and control strategies.BioMed Central2021-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdf1.3 MBhttps://hdl.handle.net/20.500.12219/5099enginfo:eu-repo/semantics/altIdentifier/hdl/http://hdl.handle.net/11336/133884info:eu-repo/semantics/altIdentifier/urn/https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-020-05603-5info:eu-repo/semantics/altIdentifier/urn/https://link.springer.com/article/10.1186/s12879-020-05603-5info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 4.0 Internacionalhttp://creativecommons.org/licenses/by-nc-sa/4.0/reponame:Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM)instname:Universidad Nacional de Misiones2025-09-04T11:43:14Zoai:rid.unam.edu.ar:20.500.12219/5099instacron:UNAMInstitucionalhttps://rid.unam.edu.ar/Universidad públicahttps://www.unam.edu.ar/https://rid.unam.edu.ar/oai/rsnrdArgentinaopendoar:2025-09-04 11:43:14.382Repositorio Institucional Digital de la Universidad Nacional de Misiones (UNaM) - Universidad Nacional de Misionesfalse
dc.title.none.fl_str_mv Prevalence, serotypes and virulence genes of streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation
title Prevalence, serotypes and virulence genes of streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation
spellingShingle Prevalence, serotypes and virulence genes of streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation
Bobadilla, Fernando Javier
GBS
Colonization
Serotypes
Virulence genes
title_short Prevalence, serotypes and virulence genes of streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation
title_full Prevalence, serotypes and virulence genes of streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation
title_fullStr Prevalence, serotypes and virulence genes of streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation
title_full_unstemmed Prevalence, serotypes and virulence genes of streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation
title_sort Prevalence, serotypes and virulence genes of streptococcus agalactiae isolated from pregnant women with 35–37 weeks of gestation
dc.creator.none.fl_str_mv Bobadilla, Fernando Javier
Novosak, Marina Gisel
Cortese, Iliana Julieta
Delgado, Osvaldo Daniel
Laczeski, Margarita Ester
author Bobadilla, Fernando Javier
author_facet Bobadilla, Fernando Javier
Novosak, Marina Gisel
Cortese, Iliana Julieta
Delgado, Osvaldo Daniel
Laczeski, Margarita Ester
author_role author
author2 Novosak, Marina Gisel
Cortese, Iliana Julieta
Delgado, Osvaldo Daniel
Laczeski, Margarita Ester
author2_role author
author
author
author
dc.subject.none.fl_str_mv GBS
Colonization
Serotypes
Virulence genes
topic GBS
Colonization
Serotypes
Virulence genes
dc.description.none.fl_txt_mv Fil: Bobadilla, Fernando Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Bobadilla, Fernando Javier. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina.
Fil: Novosak, Marina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Novosak, Marina Gisel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina.
Fil: Novosak, Marina Gisel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología de Misiones; Argentina.
Fil: Novosak, Marina Gisel. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología de Misiones; Argentina.
Fil: Cortese, Iliana Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología de Misiones; Argentina.
Fil: Cortese, Iliana Julieta. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología de Misiones; Argentina.
Fil: Delgado, Osvaldo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Planta Piloto de Procesos Industriales Microbiológicos; Argentina.
Fil: Delgado, Osvaldo Daniel. Universidad Nacional de Catamarca. Planta Piloto de Procesos Industriales Microbiológicos; Argentina.
Fil: Laczeski, Margarita Ester. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Laczeski, Margarita Ester. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Departamento de Microbiología; Argentina.
Fil: Laczeski, Margarita Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología de Misiones; Argentina.
Fil: Laczeski, Margarita Ester. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biotecnología de Misiones; Argentina.
Background: In pregnant women Streptococcus agalactiae (GBS) can be transmitted to newborn causing severe infections. It is classified into 10 serotypes (Ia, Ib, II-IX). The severity of neonatal disease is determined by the capsular serotype and virulence factors such as the polysaccharide capsule, encoded by the cps gene, protein C, which includes the Cα surface proteins (bca gene), Rib (rib gene) and Cβ (bac gene); the proteins Lmb (lmb gene), FbsB (fbsB gene), FbsA (fbsA gene), the cyl operon encoding a β-hemolysin (hylB gene), the CAMP factor (cfb gene) and the C5a peptidase (scpB gene). The aim of this work was to determine the degree of GBS colonization in pregnant women, the serotypes distribution and to investigate virulence-associated genes. Methods: We worked with 3480 samples of vagino-rectal swabs of women with 35–37 weeks of gestation. The identification of the strains was carried out using conventional biochemical tests and group confirmatory serology using a commercial latex particle agglutination kit. Two hundred GBS strains were selected. Their serotype was determined by agglutination tests. The monoplex PCR technique was used to investigate nine virulence-associated genes (cps, bca, rib, bac, lmb, fbsB, fbsA, hylB and scpB). Results: The maternal colonization was 9.09%. The serotypes found were: Ia (33.50%), III (19.00%), Ib (15.50%), II (14.00%), V (7.00%) and IX (5.50%). 5.50% of strains were found to be non-serotypeable (NT). The nine virulence genes investigated were detected simultaneously in 36.50% of the strains. The genes that were most frequently detected were scpB (100.00%), fbsA (100.00%), fbsB (100.00%), cylB (95.00%), lmb (94.00%) and bca (87.50%). We found associations between serotype and genes bac (p = 0.003), cylB (p = 0.02), rib (p = 0.01) and lmb (p < 0.001). Conclusions: The frequency of vaginal-rectal colonization, serotypes distribution and associated virulence genes, varies widely among geographical areas. Therefore, epidemiological surveillance is necessary to provide data to guide decision-making and planning of prevention and control strategies.
description Fil: Bobadilla, Fernando Javier. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12219/5099
url https://hdl.handle.net/20.500.12219/5099
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/hdl/http://hdl.handle.net/11336/133884
info:eu-repo/semantics/altIdentifier/urn/https://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-020-05603-5
info:eu-repo/semantics/altIdentifier/urn/https://link.springer.com/article/10.1186/s12879-020-05603-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 4.0 Internacional
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dc.publisher.none.fl_str_mv BioMed Central
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