Taguchi method for the optimization of three loop mediated isothermal amplification procedures for Tritrichomonas foetus detection

Autores
Ramirez, Maria Rosana
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Ramirez, Maria Rosana. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.
Taguchi method was used to optimize loop mediated isothermal amplification tests aimed to amplify segments of the elongation factor 1a1 (tf-ef1a1), the 5,8 ribosomal gene (tf-5,8 r) or the beta tubulin 2 (tf-btub2) from the protozoan parasite Tritrichomonas foetus. L9 orthogonal array and quadratic loss functions that penalize deviations from prediction values revealed the effect of amplification reaction components. Analysis of variance (ANOVA) decomposed the contribution of individual factors to a small Ct. Confirmation experiments established that optimum conditions were predictable, verifiable and reproducible. Primers concentration conditioned the non-specific amplification of tf-ef1a1 while betaine and magnesium concentration contributed to accelerate the time to reach a positive threshold in tf-ef1a1, tf-5,8 r and tf-btub2. The general strategy of simple and robust experimental design holds potential as a general optimization protocol for LAMP tests in every diagnostic laboratory.
Materia
ARTICULO
NUTRICION
TRITRICHOMONAS FOETUS
METODO TAGUCHI
MEDICINA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Institucional (Fundacion Barceló)
Institución
Fundación H. A. Barceló
OAI Identificador
oai:fbarcelo:snrd:HASHa09b9c1c7e59f75bbc5ee0
Acceder
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network_acronym_str RIBARCELO
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network_name_str Repositorio Institucional (Fundacion Barceló)
spelling Taguchi method for the optimization of three loop mediated isothermal amplification procedures for Tritrichomonas foetus detectionRamirez, Maria RosanaARTICULONUTRICIONTRITRICHOMONAS FOETUSMETODO TAGUCHIMEDICINAFil: Ramirez, Maria Rosana. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.Taguchi method was used to optimize loop mediated isothermal amplification tests aimed to amplify segments of the elongation factor 1a1 (tf-ef1a1), the 5,8 ribosomal gene (tf-5,8 r) or the beta tubulin 2 (tf-btub2) from the protozoan parasite Tritrichomonas foetus. L9 orthogonal array and quadratic loss functions that penalize deviations from prediction values revealed the effect of amplification reaction components. Analysis of variance (ANOVA) decomposed the contribution of individual factors to a small Ct. Confirmation experiments established that optimum conditions were predictable, verifiable and reproducible. Primers concentration conditioned the non-specific amplification of tf-ef1a1 while betaine and magnesium concentration contributed to accelerate the time to reach a positive threshold in tf-ef1a1, tf-5,8 r and tf-btub2. The general strategy of simple and robust experimental design holds potential as a general optimization protocol for LAMP tests in every diagnostic laboratory.Veterinary Parasitology 295 (2021) 109462Morero, MarianaOyhenart, Jorge2021-07-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://repositorio.barcelo.edu.ar/greenstone/collect/snrd/index/assoc/HASHa09b.dir/BRC_124_MED_NUT_BA.pdfenginfo:eu-repo/semantics/openAccessreponame:Repositorio Institucional (Fundacion Barceló)instname:Fundación H. A. Barceló2025-09-18T10:48:56Zoai:fbarcelo:snrd:HASHa09b9c1c7e59f75bbc5ee0instacron:BARCELOInstitucionalhttp://repositorio.barcelo.edu.ar/greenstone/cgi-bin/library.cgiUniversidad privadaNo correspondehttp://repositorio.barcelo.edu.ar/greenstone/cgi-bin/oaiserver.cgilrodriguezares@barcelo.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-18 10:48:57.536Repositorio Institucional (Fundacion Barceló) - Fundación H. A. Barcelófalse
dc.title.none.fl_str_mv Taguchi method for the optimization of three loop mediated isothermal amplification procedures for Tritrichomonas foetus detection
title Taguchi method for the optimization of three loop mediated isothermal amplification procedures for Tritrichomonas foetus detection
spellingShingle Taguchi method for the optimization of three loop mediated isothermal amplification procedures for Tritrichomonas foetus detection
Ramirez, Maria Rosana
ARTICULO
NUTRICION
TRITRICHOMONAS FOETUS
METODO TAGUCHI
MEDICINA
title_short Taguchi method for the optimization of three loop mediated isothermal amplification procedures for Tritrichomonas foetus detection
title_full Taguchi method for the optimization of three loop mediated isothermal amplification procedures for Tritrichomonas foetus detection
title_fullStr Taguchi method for the optimization of three loop mediated isothermal amplification procedures for Tritrichomonas foetus detection
title_full_unstemmed Taguchi method for the optimization of three loop mediated isothermal amplification procedures for Tritrichomonas foetus detection
title_sort Taguchi method for the optimization of three loop mediated isothermal amplification procedures for Tritrichomonas foetus detection
dc.creator.none.fl_str_mv Ramirez, Maria Rosana
author Ramirez, Maria Rosana
author_facet Ramirez, Maria Rosana
author_role author
dc.contributor.none.fl_str_mv Morero, Mariana
Oyhenart, Jorge
dc.subject.none.fl_str_mv ARTICULO
NUTRICION
TRITRICHOMONAS FOETUS
METODO TAGUCHI
MEDICINA
topic ARTICULO
NUTRICION
TRITRICHOMONAS FOETUS
METODO TAGUCHI
MEDICINA
dc.description.none.fl_txt_mv Fil: Ramirez, Maria Rosana. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.
Taguchi method was used to optimize loop mediated isothermal amplification tests aimed to amplify segments of the elongation factor 1a1 (tf-ef1a1), the 5,8 ribosomal gene (tf-5,8 r) or the beta tubulin 2 (tf-btub2) from the protozoan parasite Tritrichomonas foetus. L9 orthogonal array and quadratic loss functions that penalize deviations from prediction values revealed the effect of amplification reaction components. Analysis of variance (ANOVA) decomposed the contribution of individual factors to a small Ct. Confirmation experiments established that optimum conditions were predictable, verifiable and reproducible. Primers concentration conditioned the non-specific amplification of tf-ef1a1 while betaine and magnesium concentration contributed to accelerate the time to reach a positive threshold in tf-ef1a1, tf-5,8 r and tf-btub2. The general strategy of simple and robust experimental design holds potential as a general optimization protocol for LAMP tests in every diagnostic laboratory.
description Fil: Ramirez, Maria Rosana. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.
publishDate 2021
dc.date.none.fl_str_mv 2021-07-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://repositorio.barcelo.edu.ar/greenstone/collect/snrd/index/assoc/HASHa09b.dir/BRC_124_MED_NUT_BA.pdf
url http://repositorio.barcelo.edu.ar/greenstone/collect/snrd/index/assoc/HASHa09b.dir/BRC_124_MED_NUT_BA.pdf
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Veterinary Parasitology 295 (2021) 109462
publisher.none.fl_str_mv Veterinary Parasitology 295 (2021) 109462
dc.source.none.fl_str_mv reponame:Repositorio Institucional (Fundacion Barceló)
instname:Fundación H. A. Barceló
reponame_str Repositorio Institucional (Fundacion Barceló)
collection Repositorio Institucional (Fundacion Barceló)
instname_str Fundación H. A. Barceló
repository.name.fl_str_mv Repositorio Institucional (Fundacion Barceló) - Fundación H. A. Barceló
repository.mail.fl_str_mv lrodriguezares@barcelo.edu.ar
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score 13.070432

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