Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion

Autores
Fernandez, Belisario Enrique; Choi, Marcelo Roberto
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Fernandez, Belisario Enrique. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.
Fil: Choi, Marcelo Roberto. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.
A close relationship between angiotensin II (ANG II) and the renal dopaminergic system (RDS) has been reported. Our aim was to study whether renal dopamine and ANG II can interact to modify renal sodium handling and then to elucidate the related mechanism. Anesthetized male Sprague–Dawley rats were used in experiments. ANG II, exogenous dopamine, and decynium-22 (or D-22, an isocyanine that specifically blocks electrogenic organic cation transporters, OCTs), were infused in vivo for 120 min. We analyzed renal and hemodynamic parameters, renal Na+, K+-ATPase levels, OCT activity, and urinary dopamine concentrations. We also evaluated the expression of D1 receptor, electroneutral organic cation transporters (OCTNs), and OCTs. ANG II decreased renal excretion of sodium in the presence of exogenous dopamine, increased Na+, K+-ATPase activity, and decreased the urinary dopamine concentration. D-22 treatment exacerbated the ANG II-mediated decrease in renal excretion of sodium and dopamine urine excretion but did not modify ANG II stimulation of Na+, K+- ATPase activity. The infusion of ANG II did not affect the expression of D1 receptor, OCTs, or OCTNs. However, the activity of OCTs was diminished by the presence of ANG II. Although ANG II did not alter the expression of D1 receptor, OCTs, and OCTNs in renal tissues, it modified the activity of OCTs and thereby decreased the urinary dopamine concentration, showing a novel mechanism by which ANG II decreases dopamine transport and its availability in the tubular lumen to stimulate D1 receptor. This study demonstrates a relationship between ANG II and dopamine, where both agents counteract their effects on sodium excretion.
Materia
ARTICULO
SODIO
ANGIOTENSINA
RIÑON
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Institucional (Fundacion Barceló)
Institución
Fundación H. A. Barceló
OAI Identificador
oai:fbarcelo:snrd:HASH01c3e793adedfaf4eeca69c0

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network_name_str Repositorio Institucional (Fundacion Barceló)
spelling Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretionFernandez, Belisario EnriqueChoi, Marcelo RobertoARTICULOSODIOANGIOTENSINARIÑONFil: Fernandez, Belisario Enrique. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.Fil: Choi, Marcelo Roberto. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.A close relationship between angiotensin II (ANG II) and the renal dopaminergic system (RDS) has been reported. Our aim was to study whether renal dopamine and ANG II can interact to modify renal sodium handling and then to elucidate the related mechanism. Anesthetized male Sprague–Dawley rats were used in experiments. ANG II, exogenous dopamine, and decynium-22 (or D-22, an isocyanine that specifically blocks electrogenic organic cation transporters, OCTs), were infused in vivo for 120 min. We analyzed renal and hemodynamic parameters, renal Na+, K+-ATPase levels, OCT activity, and urinary dopamine concentrations. We also evaluated the expression of D1 receptor, electroneutral organic cation transporters (OCTNs), and OCTs. ANG II decreased renal excretion of sodium in the presence of exogenous dopamine, increased Na+, K+-ATPase activity, and decreased the urinary dopamine concentration. D-22 treatment exacerbated the ANG II-mediated decrease in renal excretion of sodium and dopamine urine excretion but did not modify ANG II stimulation of Na+, K+- ATPase activity. The infusion of ANG II did not affect the expression of D1 receptor, OCTs, or OCTNs. However, the activity of OCTs was diminished by the presence of ANG II. Although ANG II did not alter the expression of D1 receptor, OCTs, and OCTNs in renal tissues, it modified the activity of OCTs and thereby decreased the urinary dopamine concentration, showing a novel mechanism by which ANG II decreases dopamine transport and its availability in the tubular lumen to stimulate D1 receptor. This study demonstrates a relationship between ANG II and dopamine, where both agents counteract their effects on sodium excretion.Hypertens Res (2020)2020-10-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://repositorio.barcelo.edu.ar/greenstone/collect/snrd/index/assoc/HASH01c3/e793aded.dir/BRC_106_MED_BS.pdfenginfo:eu-repo/semantics/openAccessreponame:Repositorio Institucional (Fundacion Barceló)instname:Fundación H. A. Barceló2025-09-04T11:11:40Zoai:fbarcelo:snrd:HASH01c3e793adedfaf4eeca69c0instacron:BARCELOInstitucionalhttp://repositorio.barcelo.edu.ar/greenstone/cgi-bin/library.cgiUniversidad privadaNo correspondehttp://repositorio.barcelo.edu.ar/greenstone/cgi-bin/oaiserver.cgilrodriguezares@barcelo.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:11:41.421Repositorio Institucional (Fundacion Barceló) - Fundación H. A. Barcelófalse
dc.title.none.fl_str_mv Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion
title Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion
spellingShingle Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion
Fernandez, Belisario Enrique
ARTICULO
SODIO
ANGIOTENSINA
RIÑON
title_short Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion
title_full Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion
title_fullStr Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion
title_full_unstemmed Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion
title_sort Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion
dc.creator.none.fl_str_mv Fernandez, Belisario Enrique
Choi, Marcelo Roberto
author Fernandez, Belisario Enrique
author_facet Fernandez, Belisario Enrique
Choi, Marcelo Roberto
author_role author
author2 Choi, Marcelo Roberto
author2_role author
dc.subject.none.fl_str_mv ARTICULO
SODIO
ANGIOTENSINA
RIÑON
topic ARTICULO
SODIO
ANGIOTENSINA
RIÑON
dc.description.none.fl_txt_mv Fil: Fernandez, Belisario Enrique. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.
Fil: Choi, Marcelo Roberto. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.
A close relationship between angiotensin II (ANG II) and the renal dopaminergic system (RDS) has been reported. Our aim was to study whether renal dopamine and ANG II can interact to modify renal sodium handling and then to elucidate the related mechanism. Anesthetized male Sprague–Dawley rats were used in experiments. ANG II, exogenous dopamine, and decynium-22 (or D-22, an isocyanine that specifically blocks electrogenic organic cation transporters, OCTs), were infused in vivo for 120 min. We analyzed renal and hemodynamic parameters, renal Na+, K+-ATPase levels, OCT activity, and urinary dopamine concentrations. We also evaluated the expression of D1 receptor, electroneutral organic cation transporters (OCTNs), and OCTs. ANG II decreased renal excretion of sodium in the presence of exogenous dopamine, increased Na+, K+-ATPase activity, and decreased the urinary dopamine concentration. D-22 treatment exacerbated the ANG II-mediated decrease in renal excretion of sodium and dopamine urine excretion but did not modify ANG II stimulation of Na+, K+- ATPase activity. The infusion of ANG II did not affect the expression of D1 receptor, OCTs, or OCTNs. However, the activity of OCTs was diminished by the presence of ANG II. Although ANG II did not alter the expression of D1 receptor, OCTs, and OCTNs in renal tissues, it modified the activity of OCTs and thereby decreased the urinary dopamine concentration, showing a novel mechanism by which ANG II decreases dopamine transport and its availability in the tubular lumen to stimulate D1 receptor. This study demonstrates a relationship between ANG II and dopamine, where both agents counteract their effects on sodium excretion.
description Fil: Fernandez, Belisario Enrique. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-27
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://repositorio.barcelo.edu.ar/greenstone/collect/snrd/index/assoc/HASH01c3/e793aded.dir/BRC_106_MED_BS.pdf
url http://repositorio.barcelo.edu.ar/greenstone/collect/snrd/index/assoc/HASH01c3/e793aded.dir/BRC_106_MED_BS.pdf
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hypertens Res (2020)
publisher.none.fl_str_mv Hypertens Res (2020)
dc.source.none.fl_str_mv reponame:Repositorio Institucional (Fundacion Barceló)
instname:Fundación H. A. Barceló
reponame_str Repositorio Institucional (Fundacion Barceló)
collection Repositorio Institucional (Fundacion Barceló)
instname_str Fundación H. A. Barceló
repository.name.fl_str_mv Repositorio Institucional (Fundacion Barceló) - Fundación H. A. Barceló
repository.mail.fl_str_mv lrodriguezares@barcelo.edu.ar
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score 12.623145