Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion
- Autores
- Fernandez, Belisario Enrique; Choi, Marcelo Roberto
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Fernandez, Belisario Enrique. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.
Fil: Choi, Marcelo Roberto. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.
A close relationship between angiotensin II (ANG II) and the renal dopaminergic system (RDS) has been reported. Our aim was to study whether renal dopamine and ANG II can interact to modify renal sodium handling and then to elucidate the related mechanism. Anesthetized male Sprague–Dawley rats were used in experiments. ANG II, exogenous dopamine, and decynium-22 (or D-22, an isocyanine that specifically blocks electrogenic organic cation transporters, OCTs), were infused in vivo for 120 min. We analyzed renal and hemodynamic parameters, renal Na+, K+-ATPase levels, OCT activity, and urinary dopamine concentrations. We also evaluated the expression of D1 receptor, electroneutral organic cation transporters (OCTNs), and OCTs. ANG II decreased renal excretion of sodium in the presence of exogenous dopamine, increased Na+, K+-ATPase activity, and decreased the urinary dopamine concentration. D-22 treatment exacerbated the ANG II-mediated decrease in renal excretion of sodium and dopamine urine excretion but did not modify ANG II stimulation of Na+, K+- ATPase activity. The infusion of ANG II did not affect the expression of D1 receptor, OCTs, or OCTNs. However, the activity of OCTs was diminished by the presence of ANG II. Although ANG II did not alter the expression of D1 receptor, OCTs, and OCTNs in renal tissues, it modified the activity of OCTs and thereby decreased the urinary dopamine concentration, showing a novel mechanism by which ANG II decreases dopamine transport and its availability in the tubular lumen to stimulate D1 receptor. This study demonstrates a relationship between ANG II and dopamine, where both agents counteract their effects on sodium excretion. - Materia
-
ARTICULO
SODIO
ANGIOTENSINA
RIÑON - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- Repositorio
- Institución
- Fundación H. A. Barceló
- OAI Identificador
- oai:fbarcelo:snrd:HASH01c3e793adedfaf4eeca69c0
Ver los metadatos del registro completo
id |
RIBARCELO_1342b8f9b4fed3c355b6c922307597f9 |
---|---|
oai_identifier_str |
oai:fbarcelo:snrd:HASH01c3e793adedfaf4eeca69c0 |
network_acronym_str |
RIBARCELO |
repository_id_str |
a |
network_name_str |
Repositorio Institucional (Fundacion Barceló) |
spelling |
Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretionFernandez, Belisario EnriqueChoi, Marcelo RobertoARTICULOSODIOANGIOTENSINARIÑONFil: Fernandez, Belisario Enrique. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.Fil: Choi, Marcelo Roberto. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina.A close relationship between angiotensin II (ANG II) and the renal dopaminergic system (RDS) has been reported. Our aim was to study whether renal dopamine and ANG II can interact to modify renal sodium handling and then to elucidate the related mechanism. Anesthetized male Sprague–Dawley rats were used in experiments. ANG II, exogenous dopamine, and decynium-22 (or D-22, an isocyanine that specifically blocks electrogenic organic cation transporters, OCTs), were infused in vivo for 120 min. We analyzed renal and hemodynamic parameters, renal Na+, K+-ATPase levels, OCT activity, and urinary dopamine concentrations. We also evaluated the expression of D1 receptor, electroneutral organic cation transporters (OCTNs), and OCTs. ANG II decreased renal excretion of sodium in the presence of exogenous dopamine, increased Na+, K+-ATPase activity, and decreased the urinary dopamine concentration. D-22 treatment exacerbated the ANG II-mediated decrease in renal excretion of sodium and dopamine urine excretion but did not modify ANG II stimulation of Na+, K+- ATPase activity. The infusion of ANG II did not affect the expression of D1 receptor, OCTs, or OCTNs. However, the activity of OCTs was diminished by the presence of ANG II. Although ANG II did not alter the expression of D1 receptor, OCTs, and OCTNs in renal tissues, it modified the activity of OCTs and thereby decreased the urinary dopamine concentration, showing a novel mechanism by which ANG II decreases dopamine transport and its availability in the tubular lumen to stimulate D1 receptor. This study demonstrates a relationship between ANG II and dopamine, where both agents counteract their effects on sodium excretion.Hypertens Res (2020)2020-10-27info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://repositorio.barcelo.edu.ar/greenstone/collect/snrd/index/assoc/HASH01c3/e793aded.dir/BRC_106_MED_BS.pdfenginfo:eu-repo/semantics/openAccessreponame:Repositorio Institucional (Fundacion Barceló)instname:Fundación H. A. Barceló2025-09-04T11:11:40Zoai:fbarcelo:snrd:HASH01c3e793adedfaf4eeca69c0instacron:BARCELOInstitucionalhttp://repositorio.barcelo.edu.ar/greenstone/cgi-bin/library.cgiUniversidad privadaNo correspondehttp://repositorio.barcelo.edu.ar/greenstone/cgi-bin/oaiserver.cgilrodriguezares@barcelo.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:a2025-09-04 11:11:41.421Repositorio Institucional (Fundacion Barceló) - Fundación H. A. Barcelófalse |
dc.title.none.fl_str_mv |
Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion |
title |
Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion |
spellingShingle |
Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion Fernandez, Belisario Enrique ARTICULO SODIO ANGIOTENSINA RIÑON |
title_short |
Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion |
title_full |
Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion |
title_fullStr |
Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion |
title_full_unstemmed |
Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion |
title_sort |
Acute infusion of angiotensin II regulates organic cation transporters function in the kidney: its impact on the renal dopaminergic system and sodium excretion |
dc.creator.none.fl_str_mv |
Fernandez, Belisario Enrique Choi, Marcelo Roberto |
author |
Fernandez, Belisario Enrique |
author_facet |
Fernandez, Belisario Enrique Choi, Marcelo Roberto |
author_role |
author |
author2 |
Choi, Marcelo Roberto |
author2_role |
author |
dc.subject.none.fl_str_mv |
ARTICULO SODIO ANGIOTENSINA RIÑON |
topic |
ARTICULO SODIO ANGIOTENSINA RIÑON |
dc.description.none.fl_txt_mv |
Fil: Fernandez, Belisario Enrique. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina. Fil: Choi, Marcelo Roberto. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina. A close relationship between angiotensin II (ANG II) and the renal dopaminergic system (RDS) has been reported. Our aim was to study whether renal dopamine and ANG II can interact to modify renal sodium handling and then to elucidate the related mechanism. Anesthetized male Sprague–Dawley rats were used in experiments. ANG II, exogenous dopamine, and decynium-22 (or D-22, an isocyanine that specifically blocks electrogenic organic cation transporters, OCTs), were infused in vivo for 120 min. We analyzed renal and hemodynamic parameters, renal Na+, K+-ATPase levels, OCT activity, and urinary dopamine concentrations. We also evaluated the expression of D1 receptor, electroneutral organic cation transporters (OCTNs), and OCTs. ANG II decreased renal excretion of sodium in the presence of exogenous dopamine, increased Na+, K+-ATPase activity, and decreased the urinary dopamine concentration. D-22 treatment exacerbated the ANG II-mediated decrease in renal excretion of sodium and dopamine urine excretion but did not modify ANG II stimulation of Na+, K+- ATPase activity. The infusion of ANG II did not affect the expression of D1 receptor, OCTs, or OCTNs. However, the activity of OCTs was diminished by the presence of ANG II. Although ANG II did not alter the expression of D1 receptor, OCTs, and OCTNs in renal tissues, it modified the activity of OCTs and thereby decreased the urinary dopamine concentration, showing a novel mechanism by which ANG II decreases dopamine transport and its availability in the tubular lumen to stimulate D1 receptor. This study demonstrates a relationship between ANG II and dopamine, where both agents counteract their effects on sodium excretion. |
description |
Fil: Fernandez, Belisario Enrique. Instituto Universitario de Ciencias de la Salud. Fundación Barceló; Argentina. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-27 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://repositorio.barcelo.edu.ar/greenstone/collect/snrd/index/assoc/HASH01c3/e793aded.dir/BRC_106_MED_BS.pdf |
url |
http://repositorio.barcelo.edu.ar/greenstone/collect/snrd/index/assoc/HASH01c3/e793aded.dir/BRC_106_MED_BS.pdf |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hypertens Res (2020) |
publisher.none.fl_str_mv |
Hypertens Res (2020) |
dc.source.none.fl_str_mv |
reponame:Repositorio Institucional (Fundacion Barceló) instname:Fundación H. A. Barceló |
reponame_str |
Repositorio Institucional (Fundacion Barceló) |
collection |
Repositorio Institucional (Fundacion Barceló) |
instname_str |
Fundación H. A. Barceló |
repository.name.fl_str_mv |
Repositorio Institucional (Fundacion Barceló) - Fundación H. A. Barceló |
repository.mail.fl_str_mv |
lrodriguezares@barcelo.edu.ar |
_version_ |
1842344000879591424 |
score |
12.623145 |