Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants

Autores
Coviella, Carlos Eduardo; Morgan, David J. W.; Trumble, John T.
Año de publicación
2000
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Fil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Laboratorio de Ecología; Argentina.
Fil: Trumble, John T. Department of Entomology, University of California; United States of America.
Fil: Coviella, Carlos Eduardo. Department of Entomology, University of California; United States of America.
Fil: Morgan, David J. W. Department of Entomology, University of California; United States of America.
Elevated atmospheric CO2 concentrations will cause plants to grow faster, lower nitrogen content per unit of plant tissue, and generate higher carbon to nitrogen (C/N) ratios. We hypothesize that production of transgenic proteins will be reduced, thus reducing the efÞciency of Bacillus thuringiensis (Bt) transgenes against insect populations. Commercially available transgenic cotton plants expressing the Cry 1Ac gene from Bt were compared with a near isogenic non-Bt cotton line in a split-plot design with two levels of atmospheric CO2 (ambient, 370 ppm and elevated, 900 ppm) incorporating a 232 factorial design with two nitrogen (N) fertilization regimes (low, 30 mg N/kg soil/wk and high, 130 mg N/kg soil/wk), and two levels of Bt (presence or absence). Bioassays using Spodoptera exigua (Hu¨ bner) and quantitative enzyme-linked immunosorbent assays for toxin content indicated reduced Bt protein production in elevated CO2. The tendency for test insects to consume more foliage from plants with lower N, caused by the elevated CO2, did not compensate for the reduction in toxin production. N fertilization regime interacted with CO2 concentration, showing that plants growing in N limited systems would produce substantially less toxin. The use of transgenic plants is becoming increasingly important and will continue to be so in the next decades. At the same time, atmospheric CO2 increase will affect the effectiveness of this strategy. These observations have implications not only for agricultural use of transgenic plants, but also for the ecological consequences of transfer of Bt toxins to closely related wild plant genotypes.
Materia
Spodoptera
Exigua
Global
Climate
Change
Carbon
Dioxide
CO2
Cotton
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
REDIUNLU (UNLu)
Institución
Universidad Nacional de Luján
OAI Identificador
oai:ri.unlu.edu.ar:rediunlu/1101

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oai_identifier_str oai:ri.unlu.edu.ar:rediunlu/1101
network_acronym_str REDIUNLU
repository_id_str w
network_name_str REDIUNLU (UNLu)
spelling Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic PlantsCoviella, Carlos EduardoMorgan, David J. W.Trumble, John T.SpodopteraExiguaGlobalClimateChangeCarbonDioxideCO2CottonFil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Laboratorio de Ecología; Argentina.Fil: Trumble, John T. Department of Entomology, University of California; United States of America.Fil: Coviella, Carlos Eduardo. Department of Entomology, University of California; United States of America.Fil: Morgan, David J. W. Department of Entomology, University of California; United States of America.Elevated atmospheric CO2 concentrations will cause plants to grow faster, lower nitrogen content per unit of plant tissue, and generate higher carbon to nitrogen (C/N) ratios. We hypothesize that production of transgenic proteins will be reduced, thus reducing the efÞciency of Bacillus thuringiensis (Bt) transgenes against insect populations. Commercially available transgenic cotton plants expressing the Cry 1Ac gene from Bt were compared with a near isogenic non-Bt cotton line in a split-plot design with two levels of atmospheric CO2 (ambient, 370 ppm and elevated, 900 ppm) incorporating a 232 factorial design with two nitrogen (N) fertilization regimes (low, 30 mg N/kg soil/wk and high, 130 mg N/kg soil/wk), and two levels of Bt (presence or absence). Bioassays using Spodoptera exigua (Hu¨ bner) and quantitative enzyme-linked immunosorbent assays for toxin content indicated reduced Bt protein production in elevated CO2. The tendency for test insects to consume more foliage from plants with lower N, caused by the elevated CO2, did not compensate for the reduction in toxin production. N fertilization regime interacted with CO2 concentration, showing that plants growing in N limited systems would produce substantially less toxin. The use of transgenic plants is becoming increasingly important and will continue to be so in the next decades. At the same time, atmospheric CO2 increase will affect the effectiveness of this strategy. These observations have implications not only for agricultural use of transgenic plants, but also for the ecological consequences of transfer of Bt toxins to closely related wild plant genotypes.Cambridge University Press, Cambridge.2022-04-21T16:52:29Z2022-04-21T16:52:29Z2000Articleinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://ri.unlu.edu.ar/xmlui/handle/rediunlu/1101spaeninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:REDIUNLU (UNLu)instname:Universidad Nacional de Luján2025-09-29T14:29:46Zoai:ri.unlu.edu.ar:rediunlu/1101instacron:UNLuInstitucionalhttps://ri.unlu.edu.arUniversidad públicaNo correspondehttps://ri.unlu.edu.ar/oaivcano@unlu.edu.ar;fgutierrez@mail.unlu.edu.ar;faquilinogutierrez@gmail.com ArgentinaNo correspondeNo correspondeNo correspondeopendoar:w2025-09-29 14:29:47.084REDIUNLU (UNLu) - Universidad Nacional de Lujánfalse
dc.title.none.fl_str_mv Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
title Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
spellingShingle Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
Coviella, Carlos Eduardo
Spodoptera
Exigua
Global
Climate
Change
Carbon
Dioxide
CO2
Cotton
title_short Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
title_full Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
title_fullStr Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
title_full_unstemmed Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
title_sort Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
dc.creator.none.fl_str_mv Coviella, Carlos Eduardo
Morgan, David J. W.
Trumble, John T.
author Coviella, Carlos Eduardo
author_facet Coviella, Carlos Eduardo
Morgan, David J. W.
Trumble, John T.
author_role author
author2 Morgan, David J. W.
Trumble, John T.
author2_role author
author
dc.subject.none.fl_str_mv Spodoptera
Exigua
Global
Climate
Change
Carbon
Dioxide
CO2
Cotton
topic Spodoptera
Exigua
Global
Climate
Change
Carbon
Dioxide
CO2
Cotton
dc.description.none.fl_txt_mv Fil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Laboratorio de Ecología; Argentina.
Fil: Trumble, John T. Department of Entomology, University of California; United States of America.
Fil: Coviella, Carlos Eduardo. Department of Entomology, University of California; United States of America.
Fil: Morgan, David J. W. Department of Entomology, University of California; United States of America.
Elevated atmospheric CO2 concentrations will cause plants to grow faster, lower nitrogen content per unit of plant tissue, and generate higher carbon to nitrogen (C/N) ratios. We hypothesize that production of transgenic proteins will be reduced, thus reducing the efÞciency of Bacillus thuringiensis (Bt) transgenes against insect populations. Commercially available transgenic cotton plants expressing the Cry 1Ac gene from Bt were compared with a near isogenic non-Bt cotton line in a split-plot design with two levels of atmospheric CO2 (ambient, 370 ppm and elevated, 900 ppm) incorporating a 232 factorial design with two nitrogen (N) fertilization regimes (low, 30 mg N/kg soil/wk and high, 130 mg N/kg soil/wk), and two levels of Bt (presence or absence). Bioassays using Spodoptera exigua (Hu¨ bner) and quantitative enzyme-linked immunosorbent assays for toxin content indicated reduced Bt protein production in elevated CO2. The tendency for test insects to consume more foliage from plants with lower N, caused by the elevated CO2, did not compensate for the reduction in toxin production. N fertilization regime interacted with CO2 concentration, showing that plants growing in N limited systems would produce substantially less toxin. The use of transgenic plants is becoming increasingly important and will continue to be so in the next decades. At the same time, atmospheric CO2 increase will affect the effectiveness of this strategy. These observations have implications not only for agricultural use of transgenic plants, but also for the ecological consequences of transfer of Bt toxins to closely related wild plant genotypes.
description Fil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Laboratorio de Ecología; Argentina.
publishDate 2000
dc.date.none.fl_str_mv 2000
2022-04-21T16:52:29Z
2022-04-21T16:52:29Z
dc.type.none.fl_str_mv Article
info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://ri.unlu.edu.ar/xmlui/handle/rediunlu/1101
url http://ri.unlu.edu.ar/xmlui/handle/rediunlu/1101
dc.language.none.fl_str_mv spa
en
language spa
language_invalid_str_mv en
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press, Cambridge.
publisher.none.fl_str_mv Cambridge University Press, Cambridge.
dc.source.none.fl_str_mv reponame:REDIUNLU (UNLu)
instname:Universidad Nacional de Luján
reponame_str REDIUNLU (UNLu)
collection REDIUNLU (UNLu)
instname_str Universidad Nacional de Luján
repository.name.fl_str_mv REDIUNLU (UNLu) - Universidad Nacional de Luján
repository.mail.fl_str_mv vcano@unlu.edu.ar;fgutierrez@mail.unlu.edu.ar;faquilinogutierrez@gmail.com
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