Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants
- Autores
- Coviella, Carlos Eduardo; Morgan, David J. W.; Trumble, John T.
- Año de publicación
- 2000
- Idioma
- español castellano
- Tipo de recurso
- artículo
- Estado
- versión aceptada
- Descripción
- Fil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Laboratorio de Ecología; Argentina.
Fil: Trumble, John T. Department of Entomology, University of California; United States of America.
Fil: Coviella, Carlos Eduardo. Department of Entomology, University of California; United States of America.
Fil: Morgan, David J. W. Department of Entomology, University of California; United States of America.
Elevated atmospheric CO2 concentrations will cause plants to grow faster, lower nitrogen content per unit of plant tissue, and generate higher carbon to nitrogen (C/N) ratios. We hypothesize that production of transgenic proteins will be reduced, thus reducing the efÞciency of Bacillus thuringiensis (Bt) transgenes against insect populations. Commercially available transgenic cotton plants expressing the Cry 1Ac gene from Bt were compared with a near isogenic non-Bt cotton line in a split-plot design with two levels of atmospheric CO2 (ambient, 370 ppm and elevated, 900 ppm) incorporating a 232 factorial design with two nitrogen (N) fertilization regimes (low, 30 mg N/kg soil/wk and high, 130 mg N/kg soil/wk), and two levels of Bt (presence or absence). Bioassays using Spodoptera exigua (Hu¨ bner) and quantitative enzyme-linked immunosorbent assays for toxin content indicated reduced Bt protein production in elevated CO2. The tendency for test insects to consume more foliage from plants with lower N, caused by the elevated CO2, did not compensate for the reduction in toxin production. N fertilization regime interacted with CO2 concentration, showing that plants growing in N limited systems would produce substantially less toxin. The use of transgenic plants is becoming increasingly important and will continue to be so in the next decades. At the same time, atmospheric CO2 increase will affect the effectiveness of this strategy. These observations have implications not only for agricultural use of transgenic plants, but also for the ecological consequences of transfer of Bt toxins to closely related wild plant genotypes. - Materia
-
Spodoptera
Exigua
Global
Climate
Change
Carbon
Dioxide
CO2
Cotton - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
- Repositorio
- Institución
- Universidad Nacional de Luján
- OAI Identificador
- oai:ri.unlu.edu.ar:rediunlu/1101
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Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic PlantsCoviella, Carlos EduardoMorgan, David J. W.Trumble, John T.SpodopteraExiguaGlobalClimateChangeCarbonDioxideCO2CottonFil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Laboratorio de Ecología; Argentina.Fil: Trumble, John T. Department of Entomology, University of California; United States of America.Fil: Coviella, Carlos Eduardo. Department of Entomology, University of California; United States of America.Fil: Morgan, David J. W. Department of Entomology, University of California; United States of America.Elevated atmospheric CO2 concentrations will cause plants to grow faster, lower nitrogen content per unit of plant tissue, and generate higher carbon to nitrogen (C/N) ratios. We hypothesize that production of transgenic proteins will be reduced, thus reducing the efÞciency of Bacillus thuringiensis (Bt) transgenes against insect populations. Commercially available transgenic cotton plants expressing the Cry 1Ac gene from Bt were compared with a near isogenic non-Bt cotton line in a split-plot design with two levels of atmospheric CO2 (ambient, 370 ppm and elevated, 900 ppm) incorporating a 232 factorial design with two nitrogen (N) fertilization regimes (low, 30 mg N/kg soil/wk and high, 130 mg N/kg soil/wk), and two levels of Bt (presence or absence). Bioassays using Spodoptera exigua (Hu¨ bner) and quantitative enzyme-linked immunosorbent assays for toxin content indicated reduced Bt protein production in elevated CO2. The tendency for test insects to consume more foliage from plants with lower N, caused by the elevated CO2, did not compensate for the reduction in toxin production. N fertilization regime interacted with CO2 concentration, showing that plants growing in N limited systems would produce substantially less toxin. The use of transgenic plants is becoming increasingly important and will continue to be so in the next decades. At the same time, atmospheric CO2 increase will affect the effectiveness of this strategy. These observations have implications not only for agricultural use of transgenic plants, but also for the ecological consequences of transfer of Bt toxins to closely related wild plant genotypes.Cambridge University Press, Cambridge.2022-04-21T16:52:29Z2022-04-21T16:52:29Z2000Articleinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://ri.unlu.edu.ar/xmlui/handle/rediunlu/1101spaeninfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:REDIUNLU (UNLu)instname:Universidad Nacional de Luján2025-09-29T14:29:46Zoai:ri.unlu.edu.ar:rediunlu/1101instacron:UNLuInstitucionalhttps://ri.unlu.edu.arUniversidad públicaNo correspondehttps://ri.unlu.edu.ar/oaivcano@unlu.edu.ar;fgutierrez@mail.unlu.edu.ar;faquilinogutierrez@gmail.com ArgentinaNo correspondeNo correspondeNo correspondeopendoar:w2025-09-29 14:29:47.084REDIUNLU (UNLu) - Universidad Nacional de Lujánfalse |
dc.title.none.fl_str_mv |
Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants |
title |
Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants |
spellingShingle |
Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants Coviella, Carlos Eduardo Spodoptera Exigua Global Climate Change Carbon Dioxide CO2 Cotton |
title_short |
Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants |
title_full |
Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants |
title_fullStr |
Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants |
title_full_unstemmed |
Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants |
title_sort |
Interactions of Elevated CO2 and Nitrogen Fertilization: Effects on Production of Bacillus thuringiensis Toxins in Transgenic Plants |
dc.creator.none.fl_str_mv |
Coviella, Carlos Eduardo Morgan, David J. W. Trumble, John T. |
author |
Coviella, Carlos Eduardo |
author_facet |
Coviella, Carlos Eduardo Morgan, David J. W. Trumble, John T. |
author_role |
author |
author2 |
Morgan, David J. W. Trumble, John T. |
author2_role |
author author |
dc.subject.none.fl_str_mv |
Spodoptera Exigua Global Climate Change Carbon Dioxide CO2 Cotton |
topic |
Spodoptera Exigua Global Climate Change Carbon Dioxide CO2 Cotton |
dc.description.none.fl_txt_mv |
Fil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Laboratorio de Ecología; Argentina. Fil: Trumble, John T. Department of Entomology, University of California; United States of America. Fil: Coviella, Carlos Eduardo. Department of Entomology, University of California; United States of America. Fil: Morgan, David J. W. Department of Entomology, University of California; United States of America. Elevated atmospheric CO2 concentrations will cause plants to grow faster, lower nitrogen content per unit of plant tissue, and generate higher carbon to nitrogen (C/N) ratios. We hypothesize that production of transgenic proteins will be reduced, thus reducing the efÞciency of Bacillus thuringiensis (Bt) transgenes against insect populations. Commercially available transgenic cotton plants expressing the Cry 1Ac gene from Bt were compared with a near isogenic non-Bt cotton line in a split-plot design with two levels of atmospheric CO2 (ambient, 370 ppm and elevated, 900 ppm) incorporating a 232 factorial design with two nitrogen (N) fertilization regimes (low, 30 mg N/kg soil/wk and high, 130 mg N/kg soil/wk), and two levels of Bt (presence or absence). Bioassays using Spodoptera exigua (Hu¨ bner) and quantitative enzyme-linked immunosorbent assays for toxin content indicated reduced Bt protein production in elevated CO2. The tendency for test insects to consume more foliage from plants with lower N, caused by the elevated CO2, did not compensate for the reduction in toxin production. N fertilization regime interacted with CO2 concentration, showing that plants growing in N limited systems would produce substantially less toxin. The use of transgenic plants is becoming increasingly important and will continue to be so in the next decades. At the same time, atmospheric CO2 increase will affect the effectiveness of this strategy. These observations have implications not only for agricultural use of transgenic plants, but also for the ecological consequences of transfer of Bt toxins to closely related wild plant genotypes. |
description |
Fil: Coviella, Carlos Eduardo. Universidad Nacional de Luján. Laboratorio de Ecología; Argentina. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000 2022-04-21T16:52:29Z 2022-04-21T16:52:29Z |
dc.type.none.fl_str_mv |
Article info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
acceptedVersion |
dc.identifier.none.fl_str_mv |
http://ri.unlu.edu.ar/xmlui/handle/rediunlu/1101 |
url |
http://ri.unlu.edu.ar/xmlui/handle/rediunlu/1101 |
dc.language.none.fl_str_mv |
spa en |
language |
spa |
language_invalid_str_mv |
en |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Cambridge University Press, Cambridge. |
publisher.none.fl_str_mv |
Cambridge University Press, Cambridge. |
dc.source.none.fl_str_mv |
reponame:REDIUNLU (UNLu) instname:Universidad Nacional de Luján |
reponame_str |
REDIUNLU (UNLu) |
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REDIUNLU (UNLu) |
instname_str |
Universidad Nacional de Luján |
repository.name.fl_str_mv |
REDIUNLU (UNLu) - Universidad Nacional de Luján |
repository.mail.fl_str_mv |
vcano@unlu.edu.ar;fgutierrez@mail.unlu.edu.ar;faquilinogutierrez@gmail.com |
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12.559606 |