P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa

Autores
Lazos, Jerónimo; Piemonte, Eduardo David; Brunotto, Mabel
Año de publicación
2018
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fil: Lazos, Jerónimo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología A; Argentina.
Fil: Piemonte, Eduardo David. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología A; Argentina.
Fil: Brunotto, Mabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Biología Celular A; Argentina.
Objective: Chronic mechanical irritation (CMI) has been proposed as risk factor for oral cancer. Epigenetic alterations, particularly methylation, have been mentioned as early events in carcinogenesis, and it has been proposed that CMI could induce them. Thus, the aim of this study is to describe p16 and MGMT methylation in a specific CMI lesion: Chronic Traumatic Ulcer (CTU). Study Design: A split-mouth design was used, and two samples per individual were taken using cytobrush: CTU lesion (according to Piemonte et al. modified criteria) and clinically normal mucosa of a contralateral site. After extracting DNA, p16 and MGMT methylation status was assessed using qPCR.Results: 27 patients were studied, mean age 59.1. The most frequent CMI factor was Functional (70%), and CMI evolution time showed an average of 18.4 months. On CTU, methylation of both p16 and MGMT presented a statistically significant difference (p<0,0001 and p <0,0005, respectively) when compared to control site.Conclusion: CMI affected sites showed consistently more methylation of p16 and MGMT than mucosa devoid of CMI. Since in each case both control and study samples were from the same individual, effects of confounders were reduced. This suggests that CMI could foster carcinogenesis through epigenetic alterations.
http://www.estomatologia.com.br/congresso-sobep2018?l=trabalhos-aprovados
Fil: Lazos, Jerónimo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología A; Argentina.
Fil: Piemonte, Eduardo David. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología A; Argentina.
Fil: Brunotto, Mabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Biología Celular A; Argentina.
Otras Ciencias de la Salud
Materia
Genes p16
Úlcera
Úlceras de la boca
Cáncer bucal
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Digital Universitario (UNC)
Institución
Universidad Nacional de Córdoba
OAI Identificador
oai:rdu.unc.edu.ar:11086/23672
Acceder
id RDUUNC_7118388aa0f4af651cf6879ecee0495d
oai_identifier_str oai:rdu.unc.edu.ar:11086/23672
network_acronym_str RDUUNC
repository_id_str 2572
network_name_str Repositorio Digital Universitario (UNC)
spelling P16 and mgmt methylation in chronic mechanical irritation of the oral mucosaLazos, JerónimoPiemonte, Eduardo DavidBrunotto, MabelGenes p16ÚlceraÚlceras de la bocaCáncer bucalFil: Lazos, Jerónimo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología A; Argentina.Fil: Piemonte, Eduardo David. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología A; Argentina.Fil: Brunotto, Mabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Biología Celular A; Argentina.Objective: Chronic mechanical irritation (CMI) has been proposed as risk factor for oral cancer. Epigenetic alterations, particularly methylation, have been mentioned as early events in carcinogenesis, and it has been proposed that CMI could induce them. Thus, the aim of this study is to describe p16 and MGMT methylation in a specific CMI lesion: Chronic Traumatic Ulcer (CTU). Study Design: A split-mouth design was used, and two samples per individual were taken using cytobrush: CTU lesion (according to Piemonte et al. modified criteria) and clinically normal mucosa of a contralateral site. After extracting DNA, p16 and MGMT methylation status was assessed using qPCR.Results: 27 patients were studied, mean age 59.1. The most frequent CMI factor was Functional (70%), and CMI evolution time showed an average of 18.4 months. On CTU, methylation of both p16 and MGMT presented a statistically significant difference (p<0,0001 and p <0,0005, respectively) when compared to control site.Conclusion: CMI affected sites showed consistently more methylation of p16 and MGMT than mucosa devoid of CMI. Since in each case both control and study samples were from the same individual, effects of confounders were reduced. This suggests that CMI could foster carcinogenesis through epigenetic alterations.http://www.estomatologia.com.br/congresso-sobep2018?l=trabalhos-aprovadosFil: Lazos, Jerónimo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología A; Argentina.Fil: Piemonte, Eduardo David. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología A; Argentina.Fil: Brunotto, Mabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Biología Celular A; Argentina.Otras Ciencias de la Salud2018info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfhttp://hdl.handle.net/11086/23672enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-10-16T09:31:10Zoai:rdu.unc.edu.ar:11086/23672Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-10-16 09:31:10.811Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse
dc.title.none.fl_str_mv P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
title P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
spellingShingle P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
Lazos, Jerónimo
Genes p16
Úlcera
Úlceras de la boca
Cáncer bucal
title_short P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
title_full P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
title_fullStr P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
title_full_unstemmed P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
title_sort P16 and mgmt methylation in chronic mechanical irritation of the oral mucosa
dc.creator.none.fl_str_mv Lazos, Jerónimo
Piemonte, Eduardo David
Brunotto, Mabel
author Lazos, Jerónimo
author_facet Lazos, Jerónimo
Piemonte, Eduardo David
Brunotto, Mabel
author_role author
author2 Piemonte, Eduardo David
Brunotto, Mabel
author2_role author
author
dc.subject.none.fl_str_mv Genes p16
Úlcera
Úlceras de la boca
Cáncer bucal
topic Genes p16
Úlcera
Úlceras de la boca
Cáncer bucal
dc.description.none.fl_txt_mv Fil: Lazos, Jerónimo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología A; Argentina.
Fil: Piemonte, Eduardo David. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología A; Argentina.
Fil: Brunotto, Mabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Biología Celular A; Argentina.
Objective: Chronic mechanical irritation (CMI) has been proposed as risk factor for oral cancer. Epigenetic alterations, particularly methylation, have been mentioned as early events in carcinogenesis, and it has been proposed that CMI could induce them. Thus, the aim of this study is to describe p16 and MGMT methylation in a specific CMI lesion: Chronic Traumatic Ulcer (CTU). Study Design: A split-mouth design was used, and two samples per individual were taken using cytobrush: CTU lesion (according to Piemonte et al. modified criteria) and clinically normal mucosa of a contralateral site. After extracting DNA, p16 and MGMT methylation status was assessed using qPCR.Results: 27 patients were studied, mean age 59.1. The most frequent CMI factor was Functional (70%), and CMI evolution time showed an average of 18.4 months. On CTU, methylation of both p16 and MGMT presented a statistically significant difference (p<0,0001 and p <0,0005, respectively) when compared to control site.Conclusion: CMI affected sites showed consistently more methylation of p16 and MGMT than mucosa devoid of CMI. Since in each case both control and study samples were from the same individual, effects of confounders were reduced. This suggests that CMI could foster carcinogenesis through epigenetic alterations.
http://www.estomatologia.com.br/congresso-sobep2018?l=trabalhos-aprovados
Fil: Lazos, Jerónimo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología A; Argentina.
Fil: Piemonte, Eduardo David. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología A; Argentina.
Fil: Brunotto, Mabel. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra Biología Celular A; Argentina.
Otras Ciencias de la Salud
description Fil: Lazos, Jerónimo. Universidad Nacional de Córdoba. Facultad de Odontología. Cátedra de Estomatología A; Argentina.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/conferenceObject
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
format conferenceObject
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11086/23672
url http://hdl.handle.net/11086/23672
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositorio Digital Universitario (UNC)
instname:Universidad Nacional de Córdoba
instacron:UNC
reponame_str Repositorio Digital Universitario (UNC)
collection Repositorio Digital Universitario (UNC)
instname_str Universidad Nacional de Córdoba
instacron_str UNC
institution UNC
repository.name.fl_str_mv Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba
repository.mail.fl_str_mv oca.unc@gmail.com
_version_ 1846143393365753856
score 12.712165

Publicaciones similares