Comparative histopathological and immunological study of two field strains of chicken anemia virus

Autores
Rimondi, Agustina; Pinto, Silvina; Olivera, Valeria Soledad; Dibarbora, Marina; Perez Filgueira, Daniel Mariano; Craig, María Isabel; Pereda, Ariel Julian
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Infection of poultry with chicken anemia virus (CAV) is implicated in several field problems in broiler flocks due to the immunosuppression generated and, consequently, the increased susceptibility to secondary infections. Recently, we have reported an increased occurrence of clinical cases caused by CAV strains distantly related to those commonly used for vaccination. In order to understand the behavior of two Argentinean CAV strains (CAV-10 and CAV-18) in two-week-old chickens, an immune and histopathological study was performed. Neither mortality nor clinical signs were observed in the infected or control groups. Thymus lobes from chickens infected with both CAV viruses were smaller compared to the negative control group. At 14 days post-infection (dpi), only chickens inoculated with CAV-10 show a severe depletion of lymphocytes in the thymus cortex and in follicles from the bursa of Fabricius. Also thymopoiesis disorders, such as reduction in the percentage of total DP (CD4 + CD8α+) thymocytes and alteration in the percentages of DP subpopulations, were more important in animals inoculated with the CAV-10 than the CAV-18 strain. In addition, only animals infected with CAV-10 show a decrease in CD8αβ splenocytes. Altogether our results show that, although both Argentinean CAV strains produce subclinical infections in chickens causing immunosuppression at 14 dpi, they might differ in their in vivo pathogenicity.
Instituto de Virología
Fil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Pinto, Silvina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Patología Aviar; Argentina
Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina
Fil: Dibarbora, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina
Fil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fuente
BMC veterinary research 45 : 102. (2014)
Materia
Chicken Anemia Virus
Chickens
Animal Diseases
Histocytological Analysis
Análisis Histocitológico
Histopathology
Infection
Virus Anemia Pollo
Pollo
Enfermedades de los Animales
Histopatología
Infección
Subclinical Infection
Double Positive Cell
Negative Control Group
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3582
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oai_identifier_str oai:localhost:20.500.12123/3582
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Comparative histopathological and immunological study of two field strains of chicken anemia virusRimondi, AgustinaPinto, SilvinaOlivera, Valeria SoledadDibarbora, MarinaPerez Filgueira, Daniel MarianoCraig, María IsabelPereda, Ariel JulianChicken Anemia VirusChickensAnimal DiseasesHistocytological AnalysisAnálisis HistocitológicoHistopathologyInfectionVirus Anemia PolloPolloEnfermedades de los AnimalesHistopatologíaInfecciónSubclinical InfectionDouble Positive CellNegative Control GroupArgentinaInfection of poultry with chicken anemia virus (CAV) is implicated in several field problems in broiler flocks due to the immunosuppression generated and, consequently, the increased susceptibility to secondary infections. Recently, we have reported an increased occurrence of clinical cases caused by CAV strains distantly related to those commonly used for vaccination. In order to understand the behavior of two Argentinean CAV strains (CAV-10 and CAV-18) in two-week-old chickens, an immune and histopathological study was performed. Neither mortality nor clinical signs were observed in the infected or control groups. Thymus lobes from chickens infected with both CAV viruses were smaller compared to the negative control group. At 14 days post-infection (dpi), only chickens inoculated with CAV-10 show a severe depletion of lymphocytes in the thymus cortex and in follicles from the bursa of Fabricius. Also thymopoiesis disorders, such as reduction in the percentage of total DP (CD4 + CD8α+) thymocytes and alteration in the percentages of DP subpopulations, were more important in animals inoculated with the CAV-10 than the CAV-18 strain. In addition, only animals infected with CAV-10 show a decrease in CD8αβ splenocytes. Altogether our results show that, although both Argentinean CAV strains produce subclinical infections in chickens causing immunosuppression at 14 dpi, they might differ in their in vivo pathogenicity.Instituto de VirologíaFil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaFil: Pinto, Silvina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Patología Aviar; ArgentinaFil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; ArgentinaFil: Dibarbora, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaFil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaFil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; ArgentinaFil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaBioMed Central2018-10-11T17:36:56Z2018-10-11T17:36:56Z2014-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3582https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-014-0102-y1297-9716https://doi.org/10.1186/s13567-014-0102-yBMC veterinary research 45 : 102. (2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:16:41Zoai:localhost:20.500.12123/3582instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:41.678INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Comparative histopathological and immunological study of two field strains of chicken anemia virus
title Comparative histopathological and immunological study of two field strains of chicken anemia virus
spellingShingle Comparative histopathological and immunological study of two field strains of chicken anemia virus
Rimondi, Agustina
Chicken Anemia Virus
Chickens
Animal Diseases
Histocytological Analysis
Análisis Histocitológico
Histopathology
Infection
Virus Anemia Pollo
Pollo
Enfermedades de los Animales
Histopatología
Infección
Subclinical Infection
Double Positive Cell
Negative Control Group
Argentina
title_short Comparative histopathological and immunological study of two field strains of chicken anemia virus
title_full Comparative histopathological and immunological study of two field strains of chicken anemia virus
title_fullStr Comparative histopathological and immunological study of two field strains of chicken anemia virus
title_full_unstemmed Comparative histopathological and immunological study of two field strains of chicken anemia virus
title_sort Comparative histopathological and immunological study of two field strains of chicken anemia virus
dc.creator.none.fl_str_mv Rimondi, Agustina
Pinto, Silvina
Olivera, Valeria Soledad
Dibarbora, Marina
Perez Filgueira, Daniel Mariano
Craig, María Isabel
Pereda, Ariel Julian
author Rimondi, Agustina
author_facet Rimondi, Agustina
Pinto, Silvina
Olivera, Valeria Soledad
Dibarbora, Marina
Perez Filgueira, Daniel Mariano
Craig, María Isabel
Pereda, Ariel Julian
author_role author
author2 Pinto, Silvina
Olivera, Valeria Soledad
Dibarbora, Marina
Perez Filgueira, Daniel Mariano
Craig, María Isabel
Pereda, Ariel Julian
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Chicken Anemia Virus
Chickens
Animal Diseases
Histocytological Analysis
Análisis Histocitológico
Histopathology
Infection
Virus Anemia Pollo
Pollo
Enfermedades de los Animales
Histopatología
Infección
Subclinical Infection
Double Positive Cell
Negative Control Group
Argentina
topic Chicken Anemia Virus
Chickens
Animal Diseases
Histocytological Analysis
Análisis Histocitológico
Histopathology
Infection
Virus Anemia Pollo
Pollo
Enfermedades de los Animales
Histopatología
Infección
Subclinical Infection
Double Positive Cell
Negative Control Group
Argentina
dc.description.none.fl_txt_mv Infection of poultry with chicken anemia virus (CAV) is implicated in several field problems in broiler flocks due to the immunosuppression generated and, consequently, the increased susceptibility to secondary infections. Recently, we have reported an increased occurrence of clinical cases caused by CAV strains distantly related to those commonly used for vaccination. In order to understand the behavior of two Argentinean CAV strains (CAV-10 and CAV-18) in two-week-old chickens, an immune and histopathological study was performed. Neither mortality nor clinical signs were observed in the infected or control groups. Thymus lobes from chickens infected with both CAV viruses were smaller compared to the negative control group. At 14 days post-infection (dpi), only chickens inoculated with CAV-10 show a severe depletion of lymphocytes in the thymus cortex and in follicles from the bursa of Fabricius. Also thymopoiesis disorders, such as reduction in the percentage of total DP (CD4 + CD8α+) thymocytes and alteration in the percentages of DP subpopulations, were more important in animals inoculated with the CAV-10 than the CAV-18 strain. In addition, only animals infected with CAV-10 show a decrease in CD8αβ splenocytes. Altogether our results show that, although both Argentinean CAV strains produce subclinical infections in chickens causing immunosuppression at 14 dpi, they might differ in their in vivo pathogenicity.
Instituto de Virología
Fil: Rimondi, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Pinto, Silvina. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Patología Aviar; Argentina
Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina
Fil: Dibarbora, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Perez Filgueira, Daniel Mariano. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina
Fil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología. Laboratorio de Aves y Porcinos; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
description Infection of poultry with chicken anemia virus (CAV) is implicated in several field problems in broiler flocks due to the immunosuppression generated and, consequently, the increased susceptibility to secondary infections. Recently, we have reported an increased occurrence of clinical cases caused by CAV strains distantly related to those commonly used for vaccination. In order to understand the behavior of two Argentinean CAV strains (CAV-10 and CAV-18) in two-week-old chickens, an immune and histopathological study was performed. Neither mortality nor clinical signs were observed in the infected or control groups. Thymus lobes from chickens infected with both CAV viruses were smaller compared to the negative control group. At 14 days post-infection (dpi), only chickens inoculated with CAV-10 show a severe depletion of lymphocytes in the thymus cortex and in follicles from the bursa of Fabricius. Also thymopoiesis disorders, such as reduction in the percentage of total DP (CD4 + CD8α+) thymocytes and alteration in the percentages of DP subpopulations, were more important in animals inoculated with the CAV-10 than the CAV-18 strain. In addition, only animals infected with CAV-10 show a decrease in CD8αβ splenocytes. Altogether our results show that, although both Argentinean CAV strains produce subclinical infections in chickens causing immunosuppression at 14 dpi, they might differ in their in vivo pathogenicity.
publishDate 2014
dc.date.none.fl_str_mv 2014-10
2018-10-11T17:36:56Z
2018-10-11T17:36:56Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3582
https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-014-0102-y
1297-9716
https://doi.org/10.1186/s13567-014-0102-y
url http://hdl.handle.net/20.500.12123/3582
https://veterinaryresearch.biomedcentral.com/articles/10.1186/s13567-014-0102-y
https://doi.org/10.1186/s13567-014-0102-y
identifier_str_mv 1297-9716
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv BMC veterinary research 45 : 102. (2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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