In vitro selection and characterization of buffelgrass somaclones with different responses to water stress

Autores
Carloni, Edgardo José; Tommasino, Exequiel Arturo; Lopez Colomba, Eliana; Ribotta, Andrea Noemi; Quiroga, Mariana Paola; Griffa, Sabrina Mariana; Grunberg, Karina
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Buffelgrass is a forage grass that reproduces mainly by apomixis. In species with this reproduction mode, in vitro selection allows the incorporation of alternatives in a breeding program. The aims were to define a protocol for in vitro selection, provide a molecular and morphological characterization of the progenies of regenerated plants, and evaluate them under water stress conditions. In the embryogenic callus induction medium (IM), the highest values of the variables fresh weight of embryogenic calli, proportion of embryogenic calli and number of regenerated seedlings (NRS) were obtained in the 25 mM mannitol treatment. The remaining concentrations of the osmotic agent (50, 75, 100 and 150 mM) had a negative effect on these variables. In the regeneration medium (RM), NRS was reduced at all mannitol concentrations. When embryogenic calli were induced and seedlings were regenerated maintaining mannitol concentrations in IM and RM, the highest NRS values were recorded at 25 mM mannitol. In vitro regenerated seedlings transplanted to an experimental plot exhibited different morphological characteristics from those of the anther donor plant. ISSR primers detected 22% of polymorphic bands and divergence between 0.20 and 0.37 in in vitro regenerated plants. Finally, water stress assays confirmed that S1 progenies exhibited a differential behavior from that of the parent material. Under 100 mM of mannitol used as selection pressure in IM or in both IM and RM, S1 progenies of two regenerated materials had higher height, fresh weight and dry weight at the end of water stress assay.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Tommasino, Exequiel Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lopez Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Ribotta, Andrea Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Quiroga, Mariana Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Griffa, Sabrina Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo de Nacional Investigaciones Científicas y Técnicas; Argentina
Fuente
Plant Cell, Tissue and Organ Culture 130 (2) : 265–277 (August 2017)
Materia
Cenchrus Ciliaris
Genotipos
Fitomejoramiento
Experimentación in Vitro
Variación Somaclonal
Apomixis
Estrés Osmótico
Genotypes
Plant Breeding
In Vitro Experimentation
Somaclonal Variation
Osmotic Stress
Pasto buffel
Estrés Hídrico
Buffel Grass
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3716
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3716
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling In vitro selection and characterization of buffelgrass somaclones with different responses to water stressCarloni, Edgardo JoséTommasino, Exequiel ArturoLopez Colomba, ElianaRibotta, Andrea NoemiQuiroga, Mariana PaolaGriffa, Sabrina MarianaGrunberg, KarinaCenchrus CiliarisGenotiposFitomejoramientoExperimentación in VitroVariación SomaclonalApomixisEstrés OsmóticoGenotypesPlant BreedingIn Vitro ExperimentationSomaclonal VariationOsmotic StressPasto buffelEstrés HídricoBuffel GrassBuffelgrass is a forage grass that reproduces mainly by apomixis. In species with this reproduction mode, in vitro selection allows the incorporation of alternatives in a breeding program. The aims were to define a protocol for in vitro selection, provide a molecular and morphological characterization of the progenies of regenerated plants, and evaluate them under water stress conditions. In the embryogenic callus induction medium (IM), the highest values of the variables fresh weight of embryogenic calli, proportion of embryogenic calli and number of regenerated seedlings (NRS) were obtained in the 25 mM mannitol treatment. The remaining concentrations of the osmotic agent (50, 75, 100 and 150 mM) had a negative effect on these variables. In the regeneration medium (RM), NRS was reduced at all mannitol concentrations. When embryogenic calli were induced and seedlings were regenerated maintaining mannitol concentrations in IM and RM, the highest NRS values were recorded at 25 mM mannitol. In vitro regenerated seedlings transplanted to an experimental plot exhibited different morphological characteristics from those of the anther donor plant. ISSR primers detected 22% of polymorphic bands and divergence between 0.20 and 0.37 in in vitro regenerated plants. Finally, water stress assays confirmed that S1 progenies exhibited a differential behavior from that of the parent material. Under 100 mM of mannitol used as selection pressure in IM or in both IM and RM, S1 progenies of two regenerated materials had higher height, fresh weight and dry weight at the end of water stress assay.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Tommasino, Exequiel Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Lopez Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Ribotta, Andrea Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Quiroga, Mariana Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Griffa, Sabrina Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo de Nacional Investigaciones Científicas y Técnicas; Argentina2018-10-26T11:48:22Z2018-10-26T11:48:22Z2017-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s11240-017-1220-9http://hdl.handle.net/20.500.12123/37160167-68571573-5044https://doi.org/10.1007/s11240-017-1220-9Plant Cell, Tissue and Organ Culture 130 (2) : 265–277 (August 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:38Zoai:localhost:20.500.12123/3716instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:39.312INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv In vitro selection and characterization of buffelgrass somaclones with different responses to water stress
title In vitro selection and characterization of buffelgrass somaclones with different responses to water stress
spellingShingle In vitro selection and characterization of buffelgrass somaclones with different responses to water stress
Carloni, Edgardo José
Cenchrus Ciliaris
Genotipos
Fitomejoramiento
Experimentación in Vitro
Variación Somaclonal
Apomixis
Estrés Osmótico
Genotypes
Plant Breeding
In Vitro Experimentation
Somaclonal Variation
Osmotic Stress
Pasto buffel
Estrés Hídrico
Buffel Grass
title_short In vitro selection and characterization of buffelgrass somaclones with different responses to water stress
title_full In vitro selection and characterization of buffelgrass somaclones with different responses to water stress
title_fullStr In vitro selection and characterization of buffelgrass somaclones with different responses to water stress
title_full_unstemmed In vitro selection and characterization of buffelgrass somaclones with different responses to water stress
title_sort In vitro selection and characterization of buffelgrass somaclones with different responses to water stress
dc.creator.none.fl_str_mv Carloni, Edgardo José
Tommasino, Exequiel Arturo
Lopez Colomba, Eliana
Ribotta, Andrea Noemi
Quiroga, Mariana Paola
Griffa, Sabrina Mariana
Grunberg, Karina
author Carloni, Edgardo José
author_facet Carloni, Edgardo José
Tommasino, Exequiel Arturo
Lopez Colomba, Eliana
Ribotta, Andrea Noemi
Quiroga, Mariana Paola
Griffa, Sabrina Mariana
Grunberg, Karina
author_role author
author2 Tommasino, Exequiel Arturo
Lopez Colomba, Eliana
Ribotta, Andrea Noemi
Quiroga, Mariana Paola
Griffa, Sabrina Mariana
Grunberg, Karina
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Cenchrus Ciliaris
Genotipos
Fitomejoramiento
Experimentación in Vitro
Variación Somaclonal
Apomixis
Estrés Osmótico
Genotypes
Plant Breeding
In Vitro Experimentation
Somaclonal Variation
Osmotic Stress
Pasto buffel
Estrés Hídrico
Buffel Grass
topic Cenchrus Ciliaris
Genotipos
Fitomejoramiento
Experimentación in Vitro
Variación Somaclonal
Apomixis
Estrés Osmótico
Genotypes
Plant Breeding
In Vitro Experimentation
Somaclonal Variation
Osmotic Stress
Pasto buffel
Estrés Hídrico
Buffel Grass
dc.description.none.fl_txt_mv Buffelgrass is a forage grass that reproduces mainly by apomixis. In species with this reproduction mode, in vitro selection allows the incorporation of alternatives in a breeding program. The aims were to define a protocol for in vitro selection, provide a molecular and morphological characterization of the progenies of regenerated plants, and evaluate them under water stress conditions. In the embryogenic callus induction medium (IM), the highest values of the variables fresh weight of embryogenic calli, proportion of embryogenic calli and number of regenerated seedlings (NRS) were obtained in the 25 mM mannitol treatment. The remaining concentrations of the osmotic agent (50, 75, 100 and 150 mM) had a negative effect on these variables. In the regeneration medium (RM), NRS was reduced at all mannitol concentrations. When embryogenic calli were induced and seedlings were regenerated maintaining mannitol concentrations in IM and RM, the highest NRS values were recorded at 25 mM mannitol. In vitro regenerated seedlings transplanted to an experimental plot exhibited different morphological characteristics from those of the anther donor plant. ISSR primers detected 22% of polymorphic bands and divergence between 0.20 and 0.37 in in vitro regenerated plants. Finally, water stress assays confirmed that S1 progenies exhibited a differential behavior from that of the parent material. Under 100 mM of mannitol used as selection pressure in IM or in both IM and RM, S1 progenies of two regenerated materials had higher height, fresh weight and dry weight at the end of water stress assay.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Carloni, Edgardo José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Tommasino, Exequiel Arturo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Lopez Colomba, Eliana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Ribotta, Andrea Noemi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Quiroga, Mariana Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Griffa, Sabrina Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Grunberg, Karina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Consejo de Nacional Investigaciones Científicas y Técnicas; Argentina
description Buffelgrass is a forage grass that reproduces mainly by apomixis. In species with this reproduction mode, in vitro selection allows the incorporation of alternatives in a breeding program. The aims were to define a protocol for in vitro selection, provide a molecular and morphological characterization of the progenies of regenerated plants, and evaluate them under water stress conditions. In the embryogenic callus induction medium (IM), the highest values of the variables fresh weight of embryogenic calli, proportion of embryogenic calli and number of regenerated seedlings (NRS) were obtained in the 25 mM mannitol treatment. The remaining concentrations of the osmotic agent (50, 75, 100 and 150 mM) had a negative effect on these variables. In the regeneration medium (RM), NRS was reduced at all mannitol concentrations. When embryogenic calli were induced and seedlings were regenerated maintaining mannitol concentrations in IM and RM, the highest NRS values were recorded at 25 mM mannitol. In vitro regenerated seedlings transplanted to an experimental plot exhibited different morphological characteristics from those of the anther donor plant. ISSR primers detected 22% of polymorphic bands and divergence between 0.20 and 0.37 in in vitro regenerated plants. Finally, water stress assays confirmed that S1 progenies exhibited a differential behavior from that of the parent material. Under 100 mM of mannitol used as selection pressure in IM or in both IM and RM, S1 progenies of two regenerated materials had higher height, fresh weight and dry weight at the end of water stress assay.
publishDate 2017
dc.date.none.fl_str_mv 2017-08
2018-10-26T11:48:22Z
2018-10-26T11:48:22Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s11240-017-1220-9
http://hdl.handle.net/20.500.12123/3716
0167-6857
1573-5044
https://doi.org/10.1007/s11240-017-1220-9
url https://link.springer.com/article/10.1007/s11240-017-1220-9
http://hdl.handle.net/20.500.12123/3716
https://doi.org/10.1007/s11240-017-1220-9
identifier_str_mv 0167-6857
1573-5044
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Plant Cell, Tissue and Organ Culture 130 (2) : 265–277 (August 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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