Gene expression and in vitro replication of bovine gammaherpesvirus type 4

Autores
Romeo, Florencia; Louge Uriarte, Enrique Leopoldo; González Altamiranda, Erika; Delgado, Santiago Germán; Pereyra, Susana Beatriz; Moran, P.; Odeon, Anselmo Carlos; Perez, Sandra; Verna, Andrea Elizabeth
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In vitro cell cultures are widely used models for dissecting cellular and molecular mechanisms that lead to certain physiological conditions and diseases. The pathogenesis of BoHV-4 in the bovine reproductive tract has been studied by conducting tests on primary cultures. However, many questions remain to be answered about the role of BoHV-4 in endometrial cells. The aim of this study was to compare the replication and gene expression of BoHV-4 in cell lines and bovine reproductive tract primary cells as an in vitro model for the study of this virus. We demonstrated that BoHV-4 strains differ in their in vitro growth kinetics and gene expression but have the same cell type preference. Our results demonstrate that BoHV-4 replicates preferentially in bovine endometrial cells (BEC). However, its replication capacity extends to various cell types, since all cells that were tested were permissive to BoHV-4 infection. The highest virus titers were obtained in BEC cells. Nevertheless, virus replication efficiency could not be fully predicted from the mRNA expression profiles. This implies that there are multiple cell-type-dependent factors and strain properties that determine the level of BoHV-4 replication. The results of this study provide relevant information about the in vitro behavior of two field isolates of BoHV-4 in different cell cultures. These findings may be useful for the design of future in vitro experiments to obtain reliable results not only about the pathogenic role of BoHV-4 in the bovine female reproductive tract but also in the development of efficient antiviral strategies.
EEA Balcarce
Fil: Romeo, Florencia. Agencia Nacional de Promoción Científica y Tecnológica; Argentina.
Fil: Romeo, Florencia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Gonzalez Altamiranda, Erika. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Gonzalez Altamiranda, Erika. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Delgado, Santiago Germán. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Morán, P. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Odeón, Anselmo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Pérez, Sandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fuente
Archives of Virology 166 : 535–544 (2021)
Materia
Herpes Virus Bovino
Replicación Viral
Expresión Génica
In Vitro
Enfermedades de los Animales
Bovine Herpesvirus
Gene Expression
Animal Diseases
Viral Replication
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/9066
Acceder
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oai_identifier_str oai:localhost:20.500.12123/9066
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Gene expression and in vitro replication of bovine gammaherpesvirus type 4Romeo, FlorenciaLouge Uriarte, Enrique LeopoldoGonzález Altamiranda, ErikaDelgado, Santiago GermánPereyra, Susana BeatrizMoran, P.Odeon, Anselmo CarlosPerez, SandraVerna, Andrea ElizabethHerpes Virus BovinoReplicación ViralExpresión GénicaIn VitroEnfermedades de los AnimalesBovine HerpesvirusGene ExpressionAnimal DiseasesViral ReplicationIn vitro cell cultures are widely used models for dissecting cellular and molecular mechanisms that lead to certain physiological conditions and diseases. The pathogenesis of BoHV-4 in the bovine reproductive tract has been studied by conducting tests on primary cultures. However, many questions remain to be answered about the role of BoHV-4 in endometrial cells. The aim of this study was to compare the replication and gene expression of BoHV-4 in cell lines and bovine reproductive tract primary cells as an in vitro model for the study of this virus. We demonstrated that BoHV-4 strains differ in their in vitro growth kinetics and gene expression but have the same cell type preference. Our results demonstrate that BoHV-4 replicates preferentially in bovine endometrial cells (BEC). However, its replication capacity extends to various cell types, since all cells that were tested were permissive to BoHV-4 infection. The highest virus titers were obtained in BEC cells. Nevertheless, virus replication efficiency could not be fully predicted from the mRNA expression profiles. This implies that there are multiple cell-type-dependent factors and strain properties that determine the level of BoHV-4 replication. The results of this study provide relevant information about the in vitro behavior of two field isolates of BoHV-4 in different cell cultures. These findings may be useful for the design of future in vitro experiments to obtain reliable results not only about the pathogenic role of BoHV-4 in the bovine female reproductive tract but also in the development of efficient antiviral strategies.EEA BalcarceFil: Romeo, Florencia. Agencia Nacional de Promoción Científica y Tecnológica; Argentina.Fil: Romeo, Florencia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Gonzalez Altamiranda, Erika. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Gonzalez Altamiranda, Erika. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Delgado, Santiago Germán. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Morán, P. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.Fil: Odeón, Anselmo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Pérez, Sandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Springer Verlag2021-04-12T15:57:55Z2021-04-12T15:57:55Z2021-02-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9066https://link.springer.com/article/10.1007/s00705-020-04898-81432-8798 (online)0304-8608 (print)https://doi.org/10.1007/s00705-020-04898-8Archives of Virology 166 : 535–544 (2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-23T11:17:33Zoai:localhost:20.500.12123/9066instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:33.592INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Gene expression and in vitro replication of bovine gammaherpesvirus type 4
title Gene expression and in vitro replication of bovine gammaherpesvirus type 4
spellingShingle Gene expression and in vitro replication of bovine gammaherpesvirus type 4
Romeo, Florencia
Herpes Virus Bovino
Replicación Viral
Expresión Génica
In Vitro
Enfermedades de los Animales
Bovine Herpesvirus
Gene Expression
Animal Diseases
Viral Replication
title_short Gene expression and in vitro replication of bovine gammaherpesvirus type 4
title_full Gene expression and in vitro replication of bovine gammaherpesvirus type 4
title_fullStr Gene expression and in vitro replication of bovine gammaherpesvirus type 4
title_full_unstemmed Gene expression and in vitro replication of bovine gammaherpesvirus type 4
title_sort Gene expression and in vitro replication of bovine gammaherpesvirus type 4
dc.creator.none.fl_str_mv Romeo, Florencia
Louge Uriarte, Enrique Leopoldo
González Altamiranda, Erika
Delgado, Santiago Germán
Pereyra, Susana Beatriz
Moran, P.
Odeon, Anselmo Carlos
Perez, Sandra
Verna, Andrea Elizabeth
author Romeo, Florencia
author_facet Romeo, Florencia
Louge Uriarte, Enrique Leopoldo
González Altamiranda, Erika
Delgado, Santiago Germán
Pereyra, Susana Beatriz
Moran, P.
Odeon, Anselmo Carlos
Perez, Sandra
Verna, Andrea Elizabeth
author_role author
author2 Louge Uriarte, Enrique Leopoldo
González Altamiranda, Erika
Delgado, Santiago Germán
Pereyra, Susana Beatriz
Moran, P.
Odeon, Anselmo Carlos
Perez, Sandra
Verna, Andrea Elizabeth
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Herpes Virus Bovino
Replicación Viral
Expresión Génica
In Vitro
Enfermedades de los Animales
Bovine Herpesvirus
Gene Expression
Animal Diseases
Viral Replication
topic Herpes Virus Bovino
Replicación Viral
Expresión Génica
In Vitro
Enfermedades de los Animales
Bovine Herpesvirus
Gene Expression
Animal Diseases
Viral Replication
dc.description.none.fl_txt_mv In vitro cell cultures are widely used models for dissecting cellular and molecular mechanisms that lead to certain physiological conditions and diseases. The pathogenesis of BoHV-4 in the bovine reproductive tract has been studied by conducting tests on primary cultures. However, many questions remain to be answered about the role of BoHV-4 in endometrial cells. The aim of this study was to compare the replication and gene expression of BoHV-4 in cell lines and bovine reproductive tract primary cells as an in vitro model for the study of this virus. We demonstrated that BoHV-4 strains differ in their in vitro growth kinetics and gene expression but have the same cell type preference. Our results demonstrate that BoHV-4 replicates preferentially in bovine endometrial cells (BEC). However, its replication capacity extends to various cell types, since all cells that were tested were permissive to BoHV-4 infection. The highest virus titers were obtained in BEC cells. Nevertheless, virus replication efficiency could not be fully predicted from the mRNA expression profiles. This implies that there are multiple cell-type-dependent factors and strain properties that determine the level of BoHV-4 replication. The results of this study provide relevant information about the in vitro behavior of two field isolates of BoHV-4 in different cell cultures. These findings may be useful for the design of future in vitro experiments to obtain reliable results not only about the pathogenic role of BoHV-4 in the bovine female reproductive tract but also in the development of efficient antiviral strategies.
EEA Balcarce
Fil: Romeo, Florencia. Agencia Nacional de Promoción Científica y Tecnológica; Argentina.
Fil: Romeo, Florencia. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Gonzalez Altamiranda, Erika. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Gonzalez Altamiranda, Erika. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Delgado, Santiago Germán. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Pereyra, Susana Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Morán, P. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Odeón, Anselmo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Pérez, Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Pérez, Sandra. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina.
Fil: Verna, Andrea Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Verna, Andrea Elizabeth. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
description In vitro cell cultures are widely used models for dissecting cellular and molecular mechanisms that lead to certain physiological conditions and diseases. The pathogenesis of BoHV-4 in the bovine reproductive tract has been studied by conducting tests on primary cultures. However, many questions remain to be answered about the role of BoHV-4 in endometrial cells. The aim of this study was to compare the replication and gene expression of BoHV-4 in cell lines and bovine reproductive tract primary cells as an in vitro model for the study of this virus. We demonstrated that BoHV-4 strains differ in their in vitro growth kinetics and gene expression but have the same cell type preference. Our results demonstrate that BoHV-4 replicates preferentially in bovine endometrial cells (BEC). However, its replication capacity extends to various cell types, since all cells that were tested were permissive to BoHV-4 infection. The highest virus titers were obtained in BEC cells. Nevertheless, virus replication efficiency could not be fully predicted from the mRNA expression profiles. This implies that there are multiple cell-type-dependent factors and strain properties that determine the level of BoHV-4 replication. The results of this study provide relevant information about the in vitro behavior of two field isolates of BoHV-4 in different cell cultures. These findings may be useful for the design of future in vitro experiments to obtain reliable results not only about the pathogenic role of BoHV-4 in the bovine female reproductive tract but also in the development of efficient antiviral strategies.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-12T15:57:55Z
2021-04-12T15:57:55Z
2021-02-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/9066
https://link.springer.com/article/10.1007/s00705-020-04898-8
1432-8798 (online)
0304-8608 (print)
https://doi.org/10.1007/s00705-020-04898-8
url http://hdl.handle.net/20.500.12123/9066
https://link.springer.com/article/10.1007/s00705-020-04898-8
https://doi.org/10.1007/s00705-020-04898-8
identifier_str_mv 1432-8798 (online)
0304-8608 (print)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Verlag
publisher.none.fl_str_mv Springer Verlag
dc.source.none.fl_str_mv Archives of Virology 166 : 535–544 (2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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