Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae

Autores
Carrasco, Franca Denise; Miranda, Victoria; Sede, Silvana M.; Bustos, Sebastian; González, Valeria; Otero, Maria Laura; Fracchia, Sebastián
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Verticillium dahliae is a soilborne pathogen that causes significant losses in olive crops in northwestern Argentina. Biological control through antagonistic microorganisms such as Trichoderma has great potential in the management of Verticillium wilt of olive. This investigation aims to isolate, identify and characterize native Trichoderma strains for biocontrol of V. dahliae. Thirty-nine Trichoderma strains were isolated from soils of olive orchards and natural areas and they were morphologically and molecularly characterized. Desired attributes for plant growth and bioprotection, such as indole acetic acid (IAA) production, endophytism and antagonistic potential against the pathogen were evaluated. Thirteen Trichoderma species were identified, belonging to the Trichoderma, Longibrachiatum, Virens, and Harzianum clades. Physiological characterization revealed that fourteen strains produced IAA while ten showed endophytic capacity. The antagonistic parameters quantified were very variable: eight strains showed high growth inhibition values (PI > 65%), while six strains reached mycoparasitism (PM) values higher than 90% for both pathogens. Three endophytic strains exhibited IAA production and antagonistic activity against V. dahliae, becoming potential candidates for bioprotection of olive orchards. Olive cultivation in the arid regions of northwestern Argentina is subjected to strong stress conditions mainly due to the type of soils with low organic matter content and water retention. Thus, selected Trichoderma strains with more than one beneficial attribute are a fundamental tool for sustainable olive cultivation, not only as antagonists of emerging pathogens but also as mitigators of abiotic stress conditions that prevail in these environments.
Instituto de Patología Vegetal
Fil: Carrasco, Franca Denise. Instituto Nacional de Tecnologıa Agropecuaria (INTA).Estacion Experimental Agropecuaria Catamarca; Argentina
Fil: Miranda, Victoria. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de la Rioja (CRILAR).Consejo Nacional de Investigaciones Científicas y Técnicas- Gobierno de La Rioja. Universidad Nacional de Catamarca.Servicio Geológico Minero Argentino.Universidad Nacional de La Rioja; Argentina
Fil: Sede, Silvana M. Instituto de Bot anica Darwinion (IBODA). Academia Nacional de Ciencias Exactas, Físicas y Naturales (ANCEFN).Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Bustos, Sebastian. Universidad Nacional de Catamarca (UNCa). Facultad de Ciencias Agrarias; Argentina
Fil: González, Valeria. Universidad Nacional de C ordoba (UNC). Facultad de Ciencias Agropecuarias (FCA); Argentina
Fil: Otero, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Otero, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Fracchia, Sebastián. Universidad de Buenos Aires. Facultas de Ciencias Exactas y Naturales. Instituto de Micologıa y Botanica (INMIBO); Argentina
Fuente
Arid Land Research and Management 38 (1) : 122-143 (2024)
Materia
Biological Control
Semi-arid Zones
Control Biológico
Zona Semiárida
Verticillium dahliae
Trichoderma
Olea europaea
Biocontrol
Olive Disease
Root Endophyte
Semiarid Arid Soils
Olivo
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/17119
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oai_identifier_str oai:localhost:20.500.12123/17119
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliaeCarrasco, Franca DeniseMiranda, VictoriaSede, Silvana M.Bustos, SebastianGonzález, ValeriaOtero, Maria LauraFracchia, SebastiánBiological ControlSemi-arid ZonesControl BiológicoZona SemiáridaVerticillium dahliaeTrichodermaOlea europaeaBiocontrolOlive DiseaseRoot EndophyteSemiarid Arid SoilsOlivoVerticillium dahliae is a soilborne pathogen that causes significant losses in olive crops in northwestern Argentina. Biological control through antagonistic microorganisms such as Trichoderma has great potential in the management of Verticillium wilt of olive. This investigation aims to isolate, identify and characterize native Trichoderma strains for biocontrol of V. dahliae. Thirty-nine Trichoderma strains were isolated from soils of olive orchards and natural areas and they were morphologically and molecularly characterized. Desired attributes for plant growth and bioprotection, such as indole acetic acid (IAA) production, endophytism and antagonistic potential against the pathogen were evaluated. Thirteen Trichoderma species were identified, belonging to the Trichoderma, Longibrachiatum, Virens, and Harzianum clades. Physiological characterization revealed that fourteen strains produced IAA while ten showed endophytic capacity. The antagonistic parameters quantified were very variable: eight strains showed high growth inhibition values (PI > 65%), while six strains reached mycoparasitism (PM) values higher than 90% for both pathogens. Three endophytic strains exhibited IAA production and antagonistic activity against V. dahliae, becoming potential candidates for bioprotection of olive orchards. Olive cultivation in the arid regions of northwestern Argentina is subjected to strong stress conditions mainly due to the type of soils with low organic matter content and water retention. Thus, selected Trichoderma strains with more than one beneficial attribute are a fundamental tool for sustainable olive cultivation, not only as antagonists of emerging pathogens but also as mitigators of abiotic stress conditions that prevail in these environments.Instituto de Patología VegetalFil: Carrasco, Franca Denise. Instituto Nacional de Tecnologıa Agropecuaria (INTA).Estacion Experimental Agropecuaria Catamarca; ArgentinaFil: Miranda, Victoria. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de la Rioja (CRILAR).Consejo Nacional de Investigaciones Científicas y Técnicas- Gobierno de La Rioja. Universidad Nacional de Catamarca.Servicio Geológico Minero Argentino.Universidad Nacional de La Rioja; ArgentinaFil: Sede, Silvana M. Instituto de Bot anica Darwinion (IBODA). Academia Nacional de Ciencias Exactas, Físicas y Naturales (ANCEFN).Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Bustos, Sebastian. Universidad Nacional de Catamarca (UNCa). Facultad de Ciencias Agrarias; ArgentinaFil: González, Valeria. Universidad Nacional de C ordoba (UNC). Facultad de Ciencias Agropecuarias (FCA); ArgentinaFil: Otero, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Otero, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Fracchia, Sebastián. Universidad de Buenos Aires. Facultas de Ciencias Exactas y Naturales. Instituto de Micologıa y Botanica (INMIBO); ArgentinaTaylor and Francis2024-03-20T10:40:20Z2024-03-20T10:40:20Z2023-07-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/17119https://www.tandfonline.com/doi/10.1080/15324982.2023.22339331532-49821532-4990https://doi.org/10.1080/15324982.2023.2233933Arid Land Research and Management 38 (1) : 122-143 (2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E4-I069-001, Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetalinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:32Zoai:localhost:20.500.12123/17119instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:33.124INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae
title Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae
spellingShingle Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae
Carrasco, Franca Denise
Biological Control
Semi-arid Zones
Control Biológico
Zona Semiárida
Verticillium dahliae
Trichoderma
Olea europaea
Biocontrol
Olive Disease
Root Endophyte
Semiarid Arid Soils
Olivo
title_short Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae
title_full Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae
title_fullStr Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae
title_full_unstemmed Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae
title_sort Screening for native Trichoderma strains as potential biocontrollers of the olive pathogen Verticillium dahliae
dc.creator.none.fl_str_mv Carrasco, Franca Denise
Miranda, Victoria
Sede, Silvana M.
Bustos, Sebastian
González, Valeria
Otero, Maria Laura
Fracchia, Sebastián
author Carrasco, Franca Denise
author_facet Carrasco, Franca Denise
Miranda, Victoria
Sede, Silvana M.
Bustos, Sebastian
González, Valeria
Otero, Maria Laura
Fracchia, Sebastián
author_role author
author2 Miranda, Victoria
Sede, Silvana M.
Bustos, Sebastian
González, Valeria
Otero, Maria Laura
Fracchia, Sebastián
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Biological Control
Semi-arid Zones
Control Biológico
Zona Semiárida
Verticillium dahliae
Trichoderma
Olea europaea
Biocontrol
Olive Disease
Root Endophyte
Semiarid Arid Soils
Olivo
topic Biological Control
Semi-arid Zones
Control Biológico
Zona Semiárida
Verticillium dahliae
Trichoderma
Olea europaea
Biocontrol
Olive Disease
Root Endophyte
Semiarid Arid Soils
Olivo
dc.description.none.fl_txt_mv Verticillium dahliae is a soilborne pathogen that causes significant losses in olive crops in northwestern Argentina. Biological control through antagonistic microorganisms such as Trichoderma has great potential in the management of Verticillium wilt of olive. This investigation aims to isolate, identify and characterize native Trichoderma strains for biocontrol of V. dahliae. Thirty-nine Trichoderma strains were isolated from soils of olive orchards and natural areas and they were morphologically and molecularly characterized. Desired attributes for plant growth and bioprotection, such as indole acetic acid (IAA) production, endophytism and antagonistic potential against the pathogen were evaluated. Thirteen Trichoderma species were identified, belonging to the Trichoderma, Longibrachiatum, Virens, and Harzianum clades. Physiological characterization revealed that fourteen strains produced IAA while ten showed endophytic capacity. The antagonistic parameters quantified were very variable: eight strains showed high growth inhibition values (PI > 65%), while six strains reached mycoparasitism (PM) values higher than 90% for both pathogens. Three endophytic strains exhibited IAA production and antagonistic activity against V. dahliae, becoming potential candidates for bioprotection of olive orchards. Olive cultivation in the arid regions of northwestern Argentina is subjected to strong stress conditions mainly due to the type of soils with low organic matter content and water retention. Thus, selected Trichoderma strains with more than one beneficial attribute are a fundamental tool for sustainable olive cultivation, not only as antagonists of emerging pathogens but also as mitigators of abiotic stress conditions that prevail in these environments.
Instituto de Patología Vegetal
Fil: Carrasco, Franca Denise. Instituto Nacional de Tecnologıa Agropecuaria (INTA).Estacion Experimental Agropecuaria Catamarca; Argentina
Fil: Miranda, Victoria. Centro Regional de Investigaciones Científicas y Transferencia Tecnológica de la Rioja (CRILAR).Consejo Nacional de Investigaciones Científicas y Técnicas- Gobierno de La Rioja. Universidad Nacional de Catamarca.Servicio Geológico Minero Argentino.Universidad Nacional de La Rioja; Argentina
Fil: Sede, Silvana M. Instituto de Bot anica Darwinion (IBODA). Academia Nacional de Ciencias Exactas, Físicas y Naturales (ANCEFN).Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Bustos, Sebastian. Universidad Nacional de Catamarca (UNCa). Facultad de Ciencias Agrarias; Argentina
Fil: González, Valeria. Universidad Nacional de C ordoba (UNC). Facultad de Ciencias Agropecuarias (FCA); Argentina
Fil: Otero, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Otero, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Fracchia, Sebastián. Universidad de Buenos Aires. Facultas de Ciencias Exactas y Naturales. Instituto de Micologıa y Botanica (INMIBO); Argentina
description Verticillium dahliae is a soilborne pathogen that causes significant losses in olive crops in northwestern Argentina. Biological control through antagonistic microorganisms such as Trichoderma has great potential in the management of Verticillium wilt of olive. This investigation aims to isolate, identify and characterize native Trichoderma strains for biocontrol of V. dahliae. Thirty-nine Trichoderma strains were isolated from soils of olive orchards and natural areas and they were morphologically and molecularly characterized. Desired attributes for plant growth and bioprotection, such as indole acetic acid (IAA) production, endophytism and antagonistic potential against the pathogen were evaluated. Thirteen Trichoderma species were identified, belonging to the Trichoderma, Longibrachiatum, Virens, and Harzianum clades. Physiological characterization revealed that fourteen strains produced IAA while ten showed endophytic capacity. The antagonistic parameters quantified were very variable: eight strains showed high growth inhibition values (PI > 65%), while six strains reached mycoparasitism (PM) values higher than 90% for both pathogens. Three endophytic strains exhibited IAA production and antagonistic activity against V. dahliae, becoming potential candidates for bioprotection of olive orchards. Olive cultivation in the arid regions of northwestern Argentina is subjected to strong stress conditions mainly due to the type of soils with low organic matter content and water retention. Thus, selected Trichoderma strains with more than one beneficial attribute are a fundamental tool for sustainable olive cultivation, not only as antagonists of emerging pathogens but also as mitigators of abiotic stress conditions that prevail in these environments.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-12
2024-03-20T10:40:20Z
2024-03-20T10:40:20Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/17119
https://www.tandfonline.com/doi/10.1080/15324982.2023.2233933
1532-4982
1532-4990
https://doi.org/10.1080/15324982.2023.2233933
url http://hdl.handle.net/20.500.12123/17119
https://www.tandfonline.com/doi/10.1080/15324982.2023.2233933
https://doi.org/10.1080/15324982.2023.2233933
identifier_str_mv 1532-4982
1532-4990
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E4-I069-001, Bioprospección y caracterización de microorganismos benéficos para la protección y producción vegetal
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis
publisher.none.fl_str_mv Taylor and Francis
dc.source.none.fl_str_mv Arid Land Research and Management 38 (1) : 122-143 (2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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