Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2
- Autores
- He, Yawen; Xu, Zhiyuan; Kasputis, Tom; Zhao, Xue; Ibañez, Itati; Pavan, Florencia; Bok, Marina; Malito, Juan Pablo; Parreño, Gladys Viviana; Yuan, Lijuan; Wright, R. Clay; Chen, Juhong
- Año de publicación
- 2023
- Idioma
- inglés
- Tipo de recurso
- artÃculo
- Estado
- versión publicada
- Descripción
- The accurate and effective detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to preventing the spread of infectious diseases and ensuring human health. Herein, a nanobody-displayed whole-cell biosensor was developed for colorimetric detection of SARS-CoV-2 spike proteins. Serving as bioreceptors, yeast surfaces were genetically engineered to display SARS-CoV-2 binding of llama-derived single-domain antibodies (nanobodies) with high capture efficiency, facilitating the concentration and purification of SARS-CoV-2. Gold nanoparticles (AuNPs) employed as signal transductions were functionalized with horseradish peroxidase (HRP) and anti-SARS monoclonal antibodies to enhance the detection sensitivity. In the presence of SARS-CoV-2 spike proteins, the sandwiched binding will be formed by linking engineered yeast, SARS-CoV-2 spike proteins, and reporter AuNPs. The colorimetric signal was generated by the enzymatic reaction of HRP and its corresponding colorimetric substrate/chromogen system. At the optimal conditions, the developed whole-cell biosensor enables the sensitive detection of SARS-CoV-2 spike proteins in a linear range from 0.01 to 1 μg/mL with a limit of detection (LOD) of 0.037 μg/mL (about 4 × 108 virion particles/mL). Furthermore, the whole-cell biosensor was demonstrated to detect the spike protein of different SARS-CoV-2 variants in human serum, providing new possibilities for the detection of future SARS-CoV-2 variants.
Instituto de VirologÃa
Fil: He, Yawen. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos
Fil: Xu, Zhiyuan. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos
Fil: Kasputis, Tom. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos
Fil: Zhao, Xue. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos
Fil: Ibañez, Itati. Universidad de Buenos Aires. Instituto de QuÃmica FÃsica de los Materiales, Medio Ambiente y EnergÃa (INQUIMAE); Argentina
Fil: Ibañez, Itati. Consejo Nacional de Investigaciones CientÃficas y Técnicas; Argentina
Fil: Pavan, Florencia. Universidad de Buenos Aires. Instituto de QuÃmica FÃsica de los Materiales, Medio Ambiente y EnergÃa (INQUIMAE); Argentina
Fil: Pavan, Florencia. Consejo Nacional de Investigaciones CientÃficas y Técnicas; Argentina
Fil: Bok, Marina. Instituto Nacional de TecnologÃa Agropecuaria (INTA). INCUINTA. Instituto de VirologÃa e Innovaciones Tecnologicas; Argentina
Fil: Bok, Marina. Consejo Nacional de Investigaciones CientÃficas y Técnicas; Argentina
Fil: Malito, Juan Pablo. Instituto Nacional de TecnologÃa Agropecuaria (INTA). INCUINTA. Instituto de VirologÃa e Innovaciones Tecnologicas; Argentina
Fil: Malito, Juan Pablo. Consejo Nacional de Investigaciones CientÃficas y Técnicas; Argentina
Fil: Parreño, Gladys Viviana. Instituto Nacional de TecnologÃa Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); Argentina
Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones CientÃficas y Técnicas; Argentina
Fil: Parreño, Gladys Viviana. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos
Fil: Yuan, Lijuan. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos
Fil: Wright, R. Clay. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos
Fil: Chen, Juhong. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos - Fuente
- ACS Applied Materials & Interfaces 15 : 37184-37192 (Agosto 2023)
- Materia
-
Biosensors
Colorimetry
Severe Acute Respiratory Syndrome Coronavirus 2
Biosensores
ColorimetrÃa
Coronavirus del SÃndrome Respiratorio Agudo Grave 2
Nanobody
Nanocuerpo
SARS-CoV-2 - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
.jpg)
- Institución
- Instituto Nacional de TecnologÃa Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/15222
Ver los metadatos del registro completo
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Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2He, YawenXu, ZhiyuanKasputis, TomZhao, XueIbañez, ItatiPavan, FlorenciaBok, MarinaMalito, Juan PabloParreño, Gladys VivianaYuan, LijuanWright, R. ClayChen, JuhongBiosensorsColorimetrySevere Acute Respiratory Syndrome Coronavirus 2BiosensoresColorimetrÃaCoronavirus del SÃndrome Respiratorio Agudo Grave 2NanobodyNanocuerpoSARS-CoV-2The accurate and effective detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to preventing the spread of infectious diseases and ensuring human health. Herein, a nanobody-displayed whole-cell biosensor was developed for colorimetric detection of SARS-CoV-2 spike proteins. Serving as bioreceptors, yeast surfaces were genetically engineered to display SARS-CoV-2 binding of llama-derived single-domain antibodies (nanobodies) with high capture efficiency, facilitating the concentration and purification of SARS-CoV-2. Gold nanoparticles (AuNPs) employed as signal transductions were functionalized with horseradish peroxidase (HRP) and anti-SARS monoclonal antibodies to enhance the detection sensitivity. In the presence of SARS-CoV-2 spike proteins, the sandwiched binding will be formed by linking engineered yeast, SARS-CoV-2 spike proteins, and reporter AuNPs. The colorimetric signal was generated by the enzymatic reaction of HRP and its corresponding colorimetric substrate/chromogen system. At the optimal conditions, the developed whole-cell biosensor enables the sensitive detection of SARS-CoV-2 spike proteins in a linear range from 0.01 to 1 μg/mL with a limit of detection (LOD) of 0.037 μg/mL (about 4 × 108 virion particles/mL). Furthermore, the whole-cell biosensor was demonstrated to detect the spike protein of different SARS-CoV-2 variants in human serum, providing new possibilities for the detection of future SARS-CoV-2 variants.Instituto de VirologÃaFil: He, Yawen. Virginia Tech. Department of Biological Systems Engineering; Estados UnidosFil: Xu, Zhiyuan. Virginia Tech. Department of Biological Systems Engineering; Estados UnidosFil: Kasputis, Tom. Virginia Tech. Department of Biological Systems Engineering; Estados UnidosFil: Zhao, Xue. Virginia Tech. Department of Biological Systems Engineering; Estados UnidosFil: Ibañez, Itati. Universidad de Buenos Aires. Instituto de QuÃmica FÃsica de los Materiales, Medio Ambiente y EnergÃa (INQUIMAE); ArgentinaFil: Ibañez, Itati. Consejo Nacional de Investigaciones CientÃficas y Técnicas; ArgentinaFil: Pavan, Florencia. Universidad de Buenos Aires. Instituto de QuÃmica FÃsica de los Materiales, Medio Ambiente y EnergÃa (INQUIMAE); ArgentinaFil: Pavan, Florencia. Consejo Nacional de Investigaciones CientÃficas y Técnicas; ArgentinaFil: Bok, Marina. Instituto Nacional de TecnologÃa Agropecuaria (INTA). INCUINTA. Instituto de VirologÃa e Innovaciones Tecnologicas; ArgentinaFil: Bok, Marina. Consejo Nacional de Investigaciones CientÃficas y Técnicas; ArgentinaFil: Malito, Juan Pablo. Instituto Nacional de TecnologÃa Agropecuaria (INTA). INCUINTA. Instituto de VirologÃa e Innovaciones Tecnologicas; ArgentinaFil: Malito, Juan Pablo. Consejo Nacional de Investigaciones CientÃficas y Técnicas; ArgentinaFil: Parreño, Gladys Viviana. Instituto Nacional de TecnologÃa Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); ArgentinaFil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones CientÃficas y Técnicas; ArgentinaFil: Parreño, Gladys Viviana. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados UnidosFil: Yuan, Lijuan. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados UnidosFil: Wright, R. Clay. Virginia Tech. Department of Biological Systems Engineering; Estados UnidosFil: Chen, Juhong. Virginia Tech. Department of Biological Systems Engineering; Estados UnidosAmerican Chemical Society2023-09-15T10:55:41Z2023-09-15T10:55:41Z2023-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15222https://pubs.acs.org/doi/10.1021/acsami.3c059001944-8252https://doi.org/10.1021/acsami.3c05900ACS Applied Materials & Interfaces 15 : 37184-37192 (Agosto 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de TecnologÃa Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:49:56Zoai:localhost:20.500.12123/15222instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo cientÃfico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:57.346INTA Digital (INTA) - Instituto Nacional de TecnologÃa Agropecuariafalse |
| dc.title.none.fl_str_mv |
Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 |
| title |
Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 |
| spellingShingle |
Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 He, Yawen Biosensors Colorimetry Severe Acute Respiratory Syndrome Coronavirus 2 Biosensores ColorimetrÃa Coronavirus del SÃndrome Respiratorio Agudo Grave 2 Nanobody Nanocuerpo SARS-CoV-2 |
| title_short |
Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 |
| title_full |
Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 |
| title_fullStr |
Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 |
| title_full_unstemmed |
Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 |
| title_sort |
Development of nanobody-displayed whole-cell biosensors for the colorimetric detection of SARS-CoV‑2 |
| dc.creator.none.fl_str_mv |
He, Yawen Xu, Zhiyuan Kasputis, Tom Zhao, Xue Ibañez, Itati Pavan, Florencia Bok, Marina Malito, Juan Pablo Parreño, Gladys Viviana Yuan, Lijuan Wright, R. Clay Chen, Juhong |
| author |
He, Yawen |
| author_facet |
He, Yawen Xu, Zhiyuan Kasputis, Tom Zhao, Xue Ibañez, Itati Pavan, Florencia Bok, Marina Malito, Juan Pablo Parreño, Gladys Viviana Yuan, Lijuan Wright, R. Clay Chen, Juhong |
| author_role |
author |
| author2 |
Xu, Zhiyuan Kasputis, Tom Zhao, Xue Ibañez, Itati Pavan, Florencia Bok, Marina Malito, Juan Pablo Parreño, Gladys Viviana Yuan, Lijuan Wright, R. Clay Chen, Juhong |
| author2_role |
author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Biosensors Colorimetry Severe Acute Respiratory Syndrome Coronavirus 2 Biosensores ColorimetrÃa Coronavirus del SÃndrome Respiratorio Agudo Grave 2 Nanobody Nanocuerpo SARS-CoV-2 |
| topic |
Biosensors Colorimetry Severe Acute Respiratory Syndrome Coronavirus 2 Biosensores ColorimetrÃa Coronavirus del SÃndrome Respiratorio Agudo Grave 2 Nanobody Nanocuerpo SARS-CoV-2 |
| dc.description.none.fl_txt_mv |
The accurate and effective detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to preventing the spread of infectious diseases and ensuring human health. Herein, a nanobody-displayed whole-cell biosensor was developed for colorimetric detection of SARS-CoV-2 spike proteins. Serving as bioreceptors, yeast surfaces were genetically engineered to display SARS-CoV-2 binding of llama-derived single-domain antibodies (nanobodies) with high capture efficiency, facilitating the concentration and purification of SARS-CoV-2. Gold nanoparticles (AuNPs) employed as signal transductions were functionalized with horseradish peroxidase (HRP) and anti-SARS monoclonal antibodies to enhance the detection sensitivity. In the presence of SARS-CoV-2 spike proteins, the sandwiched binding will be formed by linking engineered yeast, SARS-CoV-2 spike proteins, and reporter AuNPs. The colorimetric signal was generated by the enzymatic reaction of HRP and its corresponding colorimetric substrate/chromogen system. At the optimal conditions, the developed whole-cell biosensor enables the sensitive detection of SARS-CoV-2 spike proteins in a linear range from 0.01 to 1 μg/mL with a limit of detection (LOD) of 0.037 μg/mL (about 4 × 108 virion particles/mL). Furthermore, the whole-cell biosensor was demonstrated to detect the spike protein of different SARS-CoV-2 variants in human serum, providing new possibilities for the detection of future SARS-CoV-2 variants. Instituto de VirologÃa Fil: He, Yawen. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos Fil: Xu, Zhiyuan. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos Fil: Kasputis, Tom. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos Fil: Zhao, Xue. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos Fil: Ibañez, Itati. Universidad de Buenos Aires. Instituto de QuÃmica FÃsica de los Materiales, Medio Ambiente y EnergÃa (INQUIMAE); Argentina Fil: Ibañez, Itati. Consejo Nacional de Investigaciones CientÃficas y Técnicas; Argentina Fil: Pavan, Florencia. Universidad de Buenos Aires. Instituto de QuÃmica FÃsica de los Materiales, Medio Ambiente y EnergÃa (INQUIMAE); Argentina Fil: Pavan, Florencia. Consejo Nacional de Investigaciones CientÃficas y Técnicas; Argentina Fil: Bok, Marina. Instituto Nacional de TecnologÃa Agropecuaria (INTA). INCUINTA. Instituto de VirologÃa e Innovaciones Tecnologicas; Argentina Fil: Bok, Marina. Consejo Nacional de Investigaciones CientÃficas y Técnicas; Argentina Fil: Malito, Juan Pablo. Instituto Nacional de TecnologÃa Agropecuaria (INTA). INCUINTA. Instituto de VirologÃa e Innovaciones Tecnologicas; Argentina Fil: Malito, Juan Pablo. Consejo Nacional de Investigaciones CientÃficas y Técnicas; Argentina Fil: Parreño, Gladys Viviana. Instituto Nacional de TecnologÃa Agropecuaria (INTA). INCUINTA. Instituto de Virologia e Innovaciones Tecnologicas (IVIT); Argentina Fil: Parreño, Gladys Viviana. Consejo Nacional de Investigaciones CientÃficas y Técnicas; Argentina Fil: Parreño, Gladys Viviana. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Yuan, Lijuan. Virginia-Maryland College of Veterinary Medicine. Department of Biomedical Sciences and Pathobiology; Estados Unidos Fil: Wright, R. Clay. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos Fil: Chen, Juhong. Virginia Tech. Department of Biological Systems Engineering; Estados Unidos |
| description |
The accurate and effective detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential to preventing the spread of infectious diseases and ensuring human health. Herein, a nanobody-displayed whole-cell biosensor was developed for colorimetric detection of SARS-CoV-2 spike proteins. Serving as bioreceptors, yeast surfaces were genetically engineered to display SARS-CoV-2 binding of llama-derived single-domain antibodies (nanobodies) with high capture efficiency, facilitating the concentration and purification of SARS-CoV-2. Gold nanoparticles (AuNPs) employed as signal transductions were functionalized with horseradish peroxidase (HRP) and anti-SARS monoclonal antibodies to enhance the detection sensitivity. In the presence of SARS-CoV-2 spike proteins, the sandwiched binding will be formed by linking engineered yeast, SARS-CoV-2 spike proteins, and reporter AuNPs. The colorimetric signal was generated by the enzymatic reaction of HRP and its corresponding colorimetric substrate/chromogen system. At the optimal conditions, the developed whole-cell biosensor enables the sensitive detection of SARS-CoV-2 spike proteins in a linear range from 0.01 to 1 μg/mL with a limit of detection (LOD) of 0.037 μg/mL (about 4 × 108 virion particles/mL). Furthermore, the whole-cell biosensor was demonstrated to detect the spike protein of different SARS-CoV-2 variants in human serum, providing new possibilities for the detection of future SARS-CoV-2 variants. |
| publishDate |
2023 |
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2023-09-15T10:55:41Z 2023-09-15T10:55:41Z 2023-08 |
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info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
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article |
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publishedVersion |
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http://hdl.handle.net/20.500.12123/15222 https://pubs.acs.org/doi/10.1021/acsami.3c05900 1944-8252 https://doi.org/10.1021/acsami.3c05900 |
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http://hdl.handle.net/20.500.12123/15222 https://pubs.acs.org/doi/10.1021/acsami.3c05900 https://doi.org/10.1021/acsami.3c05900 |
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1944-8252 |
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eng |
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American Chemical Society |
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American Chemical Society |
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