Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson

Autores
Aparicio, Alejandro Gabriel; El Mujtar, Veronica Andrea; Azpilicueta, Maria Marta; Martinez Meier, Alejandro; Mondino, Victor Alejandro
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In the Andean-Patagonian region of Argentina, Pinus ponderosa Douglas ex C. Lawson is the most widely cultivated forest tree species. Its breeding program, started in 1998, is now progressing towards the establishment of 1.5 generation clonal seed orchards. Considering recent advances in the phylogeny and taxonomy of the species, it is necessary to delve deeper into the genetic variation of the breeding population. Here, we first aim to address a previously unresolved question concerning the putative genetic lineages (varieties) of P. ponderosa that were introduced from the USA. We assessed this question through the molecular characterization of the nad1 second intron minisatellite of the mitochondrial genome of 61 plus trees planted in 1.0 clonal seed orchards, followed by Sanger sequencing to determine mtDNA haplotypes. Then, we conducted a nursery common garden trial to determine the relative contribution of the genetic factors (lineages and clones) to the variation in early growth traits, which may reflect adaptation across native geographic ranges and are also important for nursery stock production. We found three mtDNA variants, which correspond to haplotypes H1, H5 and H8, form the North-West native range of the species. In general, the variation in early seedling growth traits was not attributable to the haplotypic groups; instead, variation among clones was high and heritable. Considering the initial performance of the breeding population, it appears feasible to manage 1.0 and 1.5 clonal seed orchards by maintaining sub-specific taxa mixed together, while assessing the potential impact of intra-specific hybridization and field performance of progeny for timber production and resilience to climate change.
EEA Bariloche
Fil: Aparicio, Alejandro Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: El Mujtar, Veronica Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: El Mujtar, Veronica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Azpilicueta, Maria Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Azpilicueta, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Martinez Meier, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: Mondino, Victor Alejandro. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Esquel; Argentina
Fuente
New Forests : 1-21 (Published: 05 March 2024)
Materia
Pinus ponderosa
Variación Genética
ADN Mitocondrial
Crecimiento
Argentina
Genetic Variation
Mitochondrial DNA
Growth
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/16975
Acceder
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oai_identifier_str oai:localhost:20.500.12123/16975
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. LawsonAparicio, Alejandro GabrielEl Mujtar, Veronica AndreaAzpilicueta, Maria MartaMartinez Meier, AlejandroMondino, Victor AlejandroPinus ponderosaVariación GenéticaADN MitocondrialCrecimientoArgentinaGenetic VariationMitochondrial DNAGrowthIn the Andean-Patagonian region of Argentina, Pinus ponderosa Douglas ex C. Lawson is the most widely cultivated forest tree species. Its breeding program, started in 1998, is now progressing towards the establishment of 1.5 generation clonal seed orchards. Considering recent advances in the phylogeny and taxonomy of the species, it is necessary to delve deeper into the genetic variation of the breeding population. Here, we first aim to address a previously unresolved question concerning the putative genetic lineages (varieties) of P. ponderosa that were introduced from the USA. We assessed this question through the molecular characterization of the nad1 second intron minisatellite of the mitochondrial genome of 61 plus trees planted in 1.0 clonal seed orchards, followed by Sanger sequencing to determine mtDNA haplotypes. Then, we conducted a nursery common garden trial to determine the relative contribution of the genetic factors (lineages and clones) to the variation in early growth traits, which may reflect adaptation across native geographic ranges and are also important for nursery stock production. We found three mtDNA variants, which correspond to haplotypes H1, H5 and H8, form the North-West native range of the species. In general, the variation in early seedling growth traits was not attributable to the haplotypic groups; instead, variation among clones was high and heritable. Considering the initial performance of the breeding population, it appears feasible to manage 1.0 and 1.5 clonal seed orchards by maintaining sub-specific taxa mixed together, while assessing the potential impact of intra-specific hybridization and field performance of progeny for timber production and resilience to climate change.EEA BarilocheFil: Aparicio, Alejandro Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; ArgentinaFil: El Mujtar, Veronica Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: El Mujtar, Veronica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Azpilicueta, Maria Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Azpilicueta, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Martinez Meier, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; ArgentinaFil: Mondino, Victor Alejandro. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Esquel; ArgentinaSpringer2024-03-08T13:23:00Z2024-03-08T13:23:00Z2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/16975https://link.springer.com/article/10.1007/s11056-024-10034-00169-42861573-5095https://doi.org/10.1007/s11056-024-10034-0New Forests : 1-21 (Published: 05 March 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNFOR-1104062/AR./Mejoramiento genético de especies forestales introducidas para usos de alto valor.info:eu-repograntAgreement/INTA/2019-PE-E6-I146-001, Mejoramiento genético de especies forestales cultivadas de rápido crecimiento: un desarrollo clave para el fortalecimiento de la foresto industria nacionalArgentina .......... (nation) (World, South America)7006477info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:23Zoai:localhost:20.500.12123/16975instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:23.856INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson
title Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson
spellingShingle Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson
Aparicio, Alejandro Gabriel
Pinus ponderosa
Variación Genética
ADN Mitocondrial
Crecimiento
Argentina
Genetic Variation
Mitochondrial DNA
Growth
title_short Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson
title_full Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson
title_fullStr Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson
title_full_unstemmed Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson
title_sort Mitochondrial DNA lineages and genetic variation of early growth traits in the Argentinean breeding population of Pinus ponderosa Douglas ex C. Lawson
dc.creator.none.fl_str_mv Aparicio, Alejandro Gabriel
El Mujtar, Veronica Andrea
Azpilicueta, Maria Marta
Martinez Meier, Alejandro
Mondino, Victor Alejandro
author Aparicio, Alejandro Gabriel
author_facet Aparicio, Alejandro Gabriel
El Mujtar, Veronica Andrea
Azpilicueta, Maria Marta
Martinez Meier, Alejandro
Mondino, Victor Alejandro
author_role author
author2 El Mujtar, Veronica Andrea
Azpilicueta, Maria Marta
Martinez Meier, Alejandro
Mondino, Victor Alejandro
author2_role author
author
author
author
dc.subject.none.fl_str_mv Pinus ponderosa
Variación Genética
ADN Mitocondrial
Crecimiento
Argentina
Genetic Variation
Mitochondrial DNA
Growth
topic Pinus ponderosa
Variación Genética
ADN Mitocondrial
Crecimiento
Argentina
Genetic Variation
Mitochondrial DNA
Growth
dc.description.none.fl_txt_mv In the Andean-Patagonian region of Argentina, Pinus ponderosa Douglas ex C. Lawson is the most widely cultivated forest tree species. Its breeding program, started in 1998, is now progressing towards the establishment of 1.5 generation clonal seed orchards. Considering recent advances in the phylogeny and taxonomy of the species, it is necessary to delve deeper into the genetic variation of the breeding population. Here, we first aim to address a previously unresolved question concerning the putative genetic lineages (varieties) of P. ponderosa that were introduced from the USA. We assessed this question through the molecular characterization of the nad1 second intron minisatellite of the mitochondrial genome of 61 plus trees planted in 1.0 clonal seed orchards, followed by Sanger sequencing to determine mtDNA haplotypes. Then, we conducted a nursery common garden trial to determine the relative contribution of the genetic factors (lineages and clones) to the variation in early growth traits, which may reflect adaptation across native geographic ranges and are also important for nursery stock production. We found three mtDNA variants, which correspond to haplotypes H1, H5 and H8, form the North-West native range of the species. In general, the variation in early seedling growth traits was not attributable to the haplotypic groups; instead, variation among clones was high and heritable. Considering the initial performance of the breeding population, it appears feasible to manage 1.0 and 1.5 clonal seed orchards by maintaining sub-specific taxa mixed together, while assessing the potential impact of intra-specific hybridization and field performance of progeny for timber production and resilience to climate change.
EEA Bariloche
Fil: Aparicio, Alejandro Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: El Mujtar, Veronica Andrea. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: El Mujtar, Veronica Andrea. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Azpilicueta, Maria Marta. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Azpilicueta, Maria Marta. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Martinez Meier, Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche; Argentina
Fil: Mondino, Victor Alejandro. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Esquel; Argentina
description In the Andean-Patagonian region of Argentina, Pinus ponderosa Douglas ex C. Lawson is the most widely cultivated forest tree species. Its breeding program, started in 1998, is now progressing towards the establishment of 1.5 generation clonal seed orchards. Considering recent advances in the phylogeny and taxonomy of the species, it is necessary to delve deeper into the genetic variation of the breeding population. Here, we first aim to address a previously unresolved question concerning the putative genetic lineages (varieties) of P. ponderosa that were introduced from the USA. We assessed this question through the molecular characterization of the nad1 second intron minisatellite of the mitochondrial genome of 61 plus trees planted in 1.0 clonal seed orchards, followed by Sanger sequencing to determine mtDNA haplotypes. Then, we conducted a nursery common garden trial to determine the relative contribution of the genetic factors (lineages and clones) to the variation in early growth traits, which may reflect adaptation across native geographic ranges and are also important for nursery stock production. We found three mtDNA variants, which correspond to haplotypes H1, H5 and H8, form the North-West native range of the species. In general, the variation in early seedling growth traits was not attributable to the haplotypic groups; instead, variation among clones was high and heritable. Considering the initial performance of the breeding population, it appears feasible to manage 1.0 and 1.5 clonal seed orchards by maintaining sub-specific taxa mixed together, while assessing the potential impact of intra-specific hybridization and field performance of progeny for timber production and resilience to climate change.
publishDate 2024
dc.date.none.fl_str_mv 2024-03-08T13:23:00Z
2024-03-08T13:23:00Z
2024-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/16975
https://link.springer.com/article/10.1007/s11056-024-10034-0
0169-4286
1573-5095
https://doi.org/10.1007/s11056-024-10034-0
url http://hdl.handle.net/20.500.12123/16975
https://link.springer.com/article/10.1007/s11056-024-10034-0
https://doi.org/10.1007/s11056-024-10034-0
identifier_str_mv 0169-4286
1573-5095
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNFOR-1104062/AR./Mejoramiento genético de especies forestales introducidas para usos de alto valor.
info:eu-repograntAgreement/INTA/2019-PE-E6-I146-001, Mejoramiento genético de especies forestales cultivadas de rápido crecimiento: un desarrollo clave para el fortalecimiento de la foresto industria nacional
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina .......... (nation) (World, South America)
7006477
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv New Forests : 1-21 (Published: 05 March 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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