A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range

Autores
Gochez, Alberto Martin; Minsavage, Gerald V.; Potnis, Neha; Canteros, Blanca Isabel; Stall, Robert E.; Jones, Jeffrey B.
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Citrus canker is caused by two Xanthomonas species, Xanthomonas citri, which has become the primary pathogen where citrus canker occurs (type A citrus canker, Xc-A), and X. fuscans pv. aurantifolii (Xfa), which consists of strains B and C. The B strain is less pathogenic than the A strain, but produces symptoms in all citrus species. The C-type cankers only infect Key lime (Citrus aurantifolia) and produce a hypersensitive reaction (HR) in grapefruit (Citrus paradisi) leaves. An avirulence gene, avrGf2, was identified in a C strain that was responsible for the HR in grapefruit. AvrGf2 is a member of XopAG effector family and shares 45% identity at amino acid level with another member of the same family, AvrGf1 from strain Xc-Aw, which was previously shown to elicit an HR in grapefruit. AvrGf2 shares sequence identity with other XopAG effectors present in Xanthomonas vasculorum, Xanthomonas campestris pv. musacearum and Pseudomonas syringae pv. tomato. Mutagenesis of avrGf2 in C strain resulted in a compatible reaction in grapefruit. There was no observable effect on virulence when Xc-A transconjugants containing either avirulence gene were inoculated on Key lime. Expression of avrGf1 or avrGf2 in Xc-A resulted in a similar phenotype following infiltration into grapefruit leaves, although the avrGf2 transconjugant elicited a faster HR and lower populations than the transconjugant containing avrGf1. Also, it was shown that all Xfa-B strains tested contain a transposon in avrGf2 that helps to explain the differences in host range between B and C strains.
EEA Bella Vista
Fil: Gochez, Alberto Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina. University of Florida. Department of Plant Pathology ; Estados Unidos
Fil: Minsavage, Gerald V. University of Florida. Department of Plant Pathology; Estados Unidos
Fil: Potnis, Neha. University of Florida. Department of Plant Pathology; Estados Unidos
Fil: Canteros, Blanca Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina
Fil: Stall, Robert E. University of Florida. Department of Plant Pathology; Estados Unidos
Fil: Jones, Jeffrey B. University of Florida. Department of Plant Pathology; Estados Unidos
Fuente
Plant pathology 64 : 1207–1214. (2015)
Materia
Avirulence
Hypersensitivity
Disease Resistance
Avirulencia
Resistencia a la Enfermedad
Citrus
Xanthomonas
Xanthomonas Fuscans
Hipersensibilidad
Citrus Canker
XopAG
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4628
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4628
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host rangeGochez, Alberto MartinMinsavage, Gerald V.Potnis, NehaCanteros, Blanca IsabelStall, Robert E.Jones, Jeffrey B.AvirulenceHypersensitivityDisease ResistanceAvirulenciaResistencia a la EnfermedadCitrusXanthomonasXanthomonas FuscansHipersensibilidadCitrus CankerXopAGCitrus canker is caused by two Xanthomonas species, Xanthomonas citri, which has become the primary pathogen where citrus canker occurs (type A citrus canker, Xc-A), and X. fuscans pv. aurantifolii (Xfa), which consists of strains B and C. The B strain is less pathogenic than the A strain, but produces symptoms in all citrus species. The C-type cankers only infect Key lime (Citrus aurantifolia) and produce a hypersensitive reaction (HR) in grapefruit (Citrus paradisi) leaves. An avirulence gene, avrGf2, was identified in a C strain that was responsible for the HR in grapefruit. AvrGf2 is a member of XopAG effector family and shares 45% identity at amino acid level with another member of the same family, AvrGf1 from strain Xc-Aw, which was previously shown to elicit an HR in grapefruit. AvrGf2 shares sequence identity with other XopAG effectors present in Xanthomonas vasculorum, Xanthomonas campestris pv. musacearum and Pseudomonas syringae pv. tomato. Mutagenesis of avrGf2 in C strain resulted in a compatible reaction in grapefruit. There was no observable effect on virulence when Xc-A transconjugants containing either avirulence gene were inoculated on Key lime. Expression of avrGf1 or avrGf2 in Xc-A resulted in a similar phenotype following infiltration into grapefruit leaves, although the avrGf2 transconjugant elicited a faster HR and lower populations than the transconjugant containing avrGf1. Also, it was shown that all Xfa-B strains tested contain a transposon in avrGf2 that helps to explain the differences in host range between B and C strains.EEA Bella VistaFil: Gochez, Alberto Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina. University of Florida. Department of Plant Pathology ; Estados UnidosFil: Minsavage, Gerald V. University of Florida. Department of Plant Pathology; Estados UnidosFil: Potnis, Neha. University of Florida. Department of Plant Pathology; Estados UnidosFil: Canteros, Blanca Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; ArgentinaFil: Stall, Robert E. University of Florida. Department of Plant Pathology; Estados UnidosFil: Jones, Jeffrey B. University of Florida. Department of Plant Pathology; Estados UnidosBritish Society for Plant Pathology2019-03-15T17:29:31Z2019-03-15T17:29:31Z2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4628https://onlinelibrary.wiley.com/doi/full/10.1111/ppa.123611365-3059 (Online)https://doi.org/10.1111/ppa.12361Plant pathology 64 : 1207–1214. (2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:36Zoai:localhost:20.500.12123/4628instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:36.566INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range
title A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range
spellingShingle A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range
Gochez, Alberto Martin
Avirulence
Hypersensitivity
Disease Resistance
Avirulencia
Resistencia a la Enfermedad
Citrus
Xanthomonas
Xanthomonas Fuscans
Hipersensibilidad
Citrus Canker
XopAG
title_short A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range
title_full A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range
title_fullStr A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range
title_full_unstemmed A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range
title_sort A functional XopAG homologue in Xanthomonas fuscans pv. aurantifolii strain C limits host range
dc.creator.none.fl_str_mv Gochez, Alberto Martin
Minsavage, Gerald V.
Potnis, Neha
Canteros, Blanca Isabel
Stall, Robert E.
Jones, Jeffrey B.
author Gochez, Alberto Martin
author_facet Gochez, Alberto Martin
Minsavage, Gerald V.
Potnis, Neha
Canteros, Blanca Isabel
Stall, Robert E.
Jones, Jeffrey B.
author_role author
author2 Minsavage, Gerald V.
Potnis, Neha
Canteros, Blanca Isabel
Stall, Robert E.
Jones, Jeffrey B.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Avirulence
Hypersensitivity
Disease Resistance
Avirulencia
Resistencia a la Enfermedad
Citrus
Xanthomonas
Xanthomonas Fuscans
Hipersensibilidad
Citrus Canker
XopAG
topic Avirulence
Hypersensitivity
Disease Resistance
Avirulencia
Resistencia a la Enfermedad
Citrus
Xanthomonas
Xanthomonas Fuscans
Hipersensibilidad
Citrus Canker
XopAG
dc.description.none.fl_txt_mv Citrus canker is caused by two Xanthomonas species, Xanthomonas citri, which has become the primary pathogen where citrus canker occurs (type A citrus canker, Xc-A), and X. fuscans pv. aurantifolii (Xfa), which consists of strains B and C. The B strain is less pathogenic than the A strain, but produces symptoms in all citrus species. The C-type cankers only infect Key lime (Citrus aurantifolia) and produce a hypersensitive reaction (HR) in grapefruit (Citrus paradisi) leaves. An avirulence gene, avrGf2, was identified in a C strain that was responsible for the HR in grapefruit. AvrGf2 is a member of XopAG effector family and shares 45% identity at amino acid level with another member of the same family, AvrGf1 from strain Xc-Aw, which was previously shown to elicit an HR in grapefruit. AvrGf2 shares sequence identity with other XopAG effectors present in Xanthomonas vasculorum, Xanthomonas campestris pv. musacearum and Pseudomonas syringae pv. tomato. Mutagenesis of avrGf2 in C strain resulted in a compatible reaction in grapefruit. There was no observable effect on virulence when Xc-A transconjugants containing either avirulence gene were inoculated on Key lime. Expression of avrGf1 or avrGf2 in Xc-A resulted in a similar phenotype following infiltration into grapefruit leaves, although the avrGf2 transconjugant elicited a faster HR and lower populations than the transconjugant containing avrGf1. Also, it was shown that all Xfa-B strains tested contain a transposon in avrGf2 that helps to explain the differences in host range between B and C strains.
EEA Bella Vista
Fil: Gochez, Alberto Martin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina. University of Florida. Department of Plant Pathology ; Estados Unidos
Fil: Minsavage, Gerald V. University of Florida. Department of Plant Pathology; Estados Unidos
Fil: Potnis, Neha. University of Florida. Department of Plant Pathology; Estados Unidos
Fil: Canteros, Blanca Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bella Vista; Argentina
Fil: Stall, Robert E. University of Florida. Department of Plant Pathology; Estados Unidos
Fil: Jones, Jeffrey B. University of Florida. Department of Plant Pathology; Estados Unidos
description Citrus canker is caused by two Xanthomonas species, Xanthomonas citri, which has become the primary pathogen where citrus canker occurs (type A citrus canker, Xc-A), and X. fuscans pv. aurantifolii (Xfa), which consists of strains B and C. The B strain is less pathogenic than the A strain, but produces symptoms in all citrus species. The C-type cankers only infect Key lime (Citrus aurantifolia) and produce a hypersensitive reaction (HR) in grapefruit (Citrus paradisi) leaves. An avirulence gene, avrGf2, was identified in a C strain that was responsible for the HR in grapefruit. AvrGf2 is a member of XopAG effector family and shares 45% identity at amino acid level with another member of the same family, AvrGf1 from strain Xc-Aw, which was previously shown to elicit an HR in grapefruit. AvrGf2 shares sequence identity with other XopAG effectors present in Xanthomonas vasculorum, Xanthomonas campestris pv. musacearum and Pseudomonas syringae pv. tomato. Mutagenesis of avrGf2 in C strain resulted in a compatible reaction in grapefruit. There was no observable effect on virulence when Xc-A transconjugants containing either avirulence gene were inoculated on Key lime. Expression of avrGf1 or avrGf2 in Xc-A resulted in a similar phenotype following infiltration into grapefruit leaves, although the avrGf2 transconjugant elicited a faster HR and lower populations than the transconjugant containing avrGf1. Also, it was shown that all Xfa-B strains tested contain a transposon in avrGf2 that helps to explain the differences in host range between B and C strains.
publishDate 2015
dc.date.none.fl_str_mv 2015
2019-03-15T17:29:31Z
2019-03-15T17:29:31Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4628
https://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12361
1365-3059 (Online)
https://doi.org/10.1111/ppa.12361
url http://hdl.handle.net/20.500.12123/4628
https://onlinelibrary.wiley.com/doi/full/10.1111/ppa.12361
https://doi.org/10.1111/ppa.12361
identifier_str_mv 1365-3059 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv British Society for Plant Pathology
publisher.none.fl_str_mv British Society for Plant Pathology
dc.source.none.fl_str_mv Plant pathology 64 : 1207–1214. (2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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