A successful vitrification technique for goat morulae conservation

Autores
Fernandez, Jimena; Bruno Galarraga, María Macarena; Lacau Megido, Isabel María; Cueto, Marcela Isabel; Gibbons, Alejandro Eduardo
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to evaluate the effect of different vitrification and warming processes on the in vitro embryo survival of caprine morulae, considering the day of recovery. A total of 136 morulae of Criolla-Neuquina goats recovered on Days 7 or 8 after sponge removal, were exposed to three different vitrification processes; V1 (n ¼ 48): glycerol (G) þ ethylene glycol (EG); V2 (n ¼ 44): EG þ 0.5 M sucrose and V3 (n ¼ 44): G þ EG þ 0.5 M sucrose. The morulae of each vitrification process were randomly assigned to three warming processes; W1 (n ¼ 45): 0.5 M sucrose at 25°C; W2 (n ¼ 44): 0.5 M sucrose at 39°C; and W3 (n ¼ 47): solution containing half the concentration of the cryoprotectants þ 0.5 M sucrose at 25°C. After, embryos were cultured in 100 mL TCM 199 drops under mineral oil, at 39°C and a 6.5% CO2 atmosphere for 72 h according to the different treatments. There were no viable embryos in V1 and V2 in none of their three respective warmings. Only V3 showed an embryo survival rate to hatched blastocyst stage of 59.1%. When considering embryo survival according to the warming processes, the survival rate was higher in V3W2 (76.9%) and V3W3 (66.7%) groups compared to the V3W1 group (37.5%; P < 0.05). The embryo survival of V3 for Day 8 after sponge removal (81.3%) was higher compared to Day 7 (46.4%; P < 0.05). In conclusion, a successful embryo survival is obtained by using a combination of cryoprotectants (G þ EG) with addition of sucrose in the vitrification process for conservation of caprine morulae in embryo transfer programs. The survival rates in vitro of vitrified-warmed morulae in goats were influenced by their recovery day. Further studies should be conducted to determine if these results are reproducible in vivo embryo transfer on field situations.
Estación Experimental Agropecuaria Bariloche
Fil: Fernandez, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Produccion Animal; Argentina
Fil: Fernandez, Jimena. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Bruno Galarraga, María Macarena. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal; Argentina
Fil: Lacau Megido, Isabel María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Biología y Medicina Experimental. Laboratorio de Regulación Hipofisaria; Argentina
Fil: Cueto, Marcela Isabel. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal; Argentina
Fil: Gibbons, Alejandro Eduardo. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal. Grupo de Reproducción y Genética; Argentina
Fuente
Theriogenology 182 : 103-109 (April 2022)
Materia
Criopreservación
Reproducción Animal
Inseminación Artificial
Caprinos
Cryopreservation
Animal Reproduction
Artificial Insemination
Goats
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13847
Acceder
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oai_identifier_str oai:localhost:20.500.12123/13847
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling A successful vitrification technique for goat morulae conservationFernandez, JimenaBruno Galarraga, María MacarenaLacau Megido, Isabel MaríaCueto, Marcela IsabelGibbons, Alejandro EduardoCriopreservaciónReproducción AnimalInseminación ArtificialCaprinosCryopreservationAnimal ReproductionArtificial InseminationGoatsThe aim of this study was to evaluate the effect of different vitrification and warming processes on the in vitro embryo survival of caprine morulae, considering the day of recovery. A total of 136 morulae of Criolla-Neuquina goats recovered on Days 7 or 8 after sponge removal, were exposed to three different vitrification processes; V1 (n ¼ 48): glycerol (G) þ ethylene glycol (EG); V2 (n ¼ 44): EG þ 0.5 M sucrose and V3 (n ¼ 44): G þ EG þ 0.5 M sucrose. The morulae of each vitrification process were randomly assigned to three warming processes; W1 (n ¼ 45): 0.5 M sucrose at 25°C; W2 (n ¼ 44): 0.5 M sucrose at 39°C; and W3 (n ¼ 47): solution containing half the concentration of the cryoprotectants þ 0.5 M sucrose at 25°C. After, embryos were cultured in 100 mL TCM 199 drops under mineral oil, at 39°C and a 6.5% CO2 atmosphere for 72 h according to the different treatments. There were no viable embryos in V1 and V2 in none of their three respective warmings. Only V3 showed an embryo survival rate to hatched blastocyst stage of 59.1%. When considering embryo survival according to the warming processes, the survival rate was higher in V3W2 (76.9%) and V3W3 (66.7%) groups compared to the V3W1 group (37.5%; P < 0.05). The embryo survival of V3 for Day 8 after sponge removal (81.3%) was higher compared to Day 7 (46.4%; P < 0.05). In conclusion, a successful embryo survival is obtained by using a combination of cryoprotectants (G þ EG) with addition of sucrose in the vitrification process for conservation of caprine morulae in embryo transfer programs. The survival rates in vitro of vitrified-warmed morulae in goats were influenced by their recovery day. Further studies should be conducted to determine if these results are reproducible in vivo embryo transfer on field situations.Estación Experimental Agropecuaria BarilocheFil: Fernandez, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Produccion Animal; ArgentinaFil: Fernandez, Jimena. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Bruno Galarraga, María Macarena. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal; ArgentinaFil: Lacau Megido, Isabel María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Biología y Medicina Experimental. Laboratorio de Regulación Hipofisaria; ArgentinaFil: Cueto, Marcela Isabel. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal; ArgentinaFil: Gibbons, Alejandro Eduardo. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal. Grupo de Reproducción y Genética; ArgentinaElsevier2023-01-09T10:16:05Z2023-01-09T10:16:05Z2022-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13847https://www.sciencedirect.com/science/article/abs/pii/S0093691X220004370093-691X1879-3231https://doi.org/10.1016/j.theriogenology.2022.02.002Theriogenology 182 : 103-109 (April 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I107-001/2019-PD-E5-I107-001/AR./Biotecnologías de la reproducción: mejora de la eficiencia reproductiva en especies de interés zootécnicoinfo:eu-repo/semantics/restrictedAccess2025-09-04T09:49:42Zoai:localhost:20.500.12123/13847instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:42.47INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A successful vitrification technique for goat morulae conservation
title A successful vitrification technique for goat morulae conservation
spellingShingle A successful vitrification technique for goat morulae conservation
Fernandez, Jimena
Criopreservación
Reproducción Animal
Inseminación Artificial
Caprinos
Cryopreservation
Animal Reproduction
Artificial Insemination
Goats
title_short A successful vitrification technique for goat morulae conservation
title_full A successful vitrification technique for goat morulae conservation
title_fullStr A successful vitrification technique for goat morulae conservation
title_full_unstemmed A successful vitrification technique for goat morulae conservation
title_sort A successful vitrification technique for goat morulae conservation
dc.creator.none.fl_str_mv Fernandez, Jimena
Bruno Galarraga, María Macarena
Lacau Megido, Isabel María
Cueto, Marcela Isabel
Gibbons, Alejandro Eduardo
author Fernandez, Jimena
author_facet Fernandez, Jimena
Bruno Galarraga, María Macarena
Lacau Megido, Isabel María
Cueto, Marcela Isabel
Gibbons, Alejandro Eduardo
author_role author
author2 Bruno Galarraga, María Macarena
Lacau Megido, Isabel María
Cueto, Marcela Isabel
Gibbons, Alejandro Eduardo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Criopreservación
Reproducción Animal
Inseminación Artificial
Caprinos
Cryopreservation
Animal Reproduction
Artificial Insemination
Goats
topic Criopreservación
Reproducción Animal
Inseminación Artificial
Caprinos
Cryopreservation
Animal Reproduction
Artificial Insemination
Goats
dc.description.none.fl_txt_mv The aim of this study was to evaluate the effect of different vitrification and warming processes on the in vitro embryo survival of caprine morulae, considering the day of recovery. A total of 136 morulae of Criolla-Neuquina goats recovered on Days 7 or 8 after sponge removal, were exposed to three different vitrification processes; V1 (n ¼ 48): glycerol (G) þ ethylene glycol (EG); V2 (n ¼ 44): EG þ 0.5 M sucrose and V3 (n ¼ 44): G þ EG þ 0.5 M sucrose. The morulae of each vitrification process were randomly assigned to three warming processes; W1 (n ¼ 45): 0.5 M sucrose at 25°C; W2 (n ¼ 44): 0.5 M sucrose at 39°C; and W3 (n ¼ 47): solution containing half the concentration of the cryoprotectants þ 0.5 M sucrose at 25°C. After, embryos were cultured in 100 mL TCM 199 drops under mineral oil, at 39°C and a 6.5% CO2 atmosphere for 72 h according to the different treatments. There were no viable embryos in V1 and V2 in none of their three respective warmings. Only V3 showed an embryo survival rate to hatched blastocyst stage of 59.1%. When considering embryo survival according to the warming processes, the survival rate was higher in V3W2 (76.9%) and V3W3 (66.7%) groups compared to the V3W1 group (37.5%; P < 0.05). The embryo survival of V3 for Day 8 after sponge removal (81.3%) was higher compared to Day 7 (46.4%; P < 0.05). In conclusion, a successful embryo survival is obtained by using a combination of cryoprotectants (G þ EG) with addition of sucrose in the vitrification process for conservation of caprine morulae in embryo transfer programs. The survival rates in vitro of vitrified-warmed morulae in goats were influenced by their recovery day. Further studies should be conducted to determine if these results are reproducible in vivo embryo transfer on field situations.
Estación Experimental Agropecuaria Bariloche
Fil: Fernandez, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Area Produccion Animal; Argentina
Fil: Fernandez, Jimena. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Bruno Galarraga, María Macarena. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal; Argentina
Fil: Lacau Megido, Isabel María. Consejo Nacional de Investigaciones Cientificas y Tecnicas. Instituto de Biología y Medicina Experimental. Laboratorio de Regulación Hipofisaria; Argentina
Fil: Cueto, Marcela Isabel. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal; Argentina
Fil: Gibbons, Alejandro Eduardo. Instituto Nacional de Tecnologia Agropecuaria (INTA). Estacion Experimental Agropecuaria Bariloche. Area Produccion Animal. Grupo de Reproducción y Genética; Argentina
description The aim of this study was to evaluate the effect of different vitrification and warming processes on the in vitro embryo survival of caprine morulae, considering the day of recovery. A total of 136 morulae of Criolla-Neuquina goats recovered on Days 7 or 8 after sponge removal, were exposed to three different vitrification processes; V1 (n ¼ 48): glycerol (G) þ ethylene glycol (EG); V2 (n ¼ 44): EG þ 0.5 M sucrose and V3 (n ¼ 44): G þ EG þ 0.5 M sucrose. The morulae of each vitrification process were randomly assigned to three warming processes; W1 (n ¼ 45): 0.5 M sucrose at 25°C; W2 (n ¼ 44): 0.5 M sucrose at 39°C; and W3 (n ¼ 47): solution containing half the concentration of the cryoprotectants þ 0.5 M sucrose at 25°C. After, embryos were cultured in 100 mL TCM 199 drops under mineral oil, at 39°C and a 6.5% CO2 atmosphere for 72 h according to the different treatments. There were no viable embryos in V1 and V2 in none of their three respective warmings. Only V3 showed an embryo survival rate to hatched blastocyst stage of 59.1%. When considering embryo survival according to the warming processes, the survival rate was higher in V3W2 (76.9%) and V3W3 (66.7%) groups compared to the V3W1 group (37.5%; P < 0.05). The embryo survival of V3 for Day 8 after sponge removal (81.3%) was higher compared to Day 7 (46.4%; P < 0.05). In conclusion, a successful embryo survival is obtained by using a combination of cryoprotectants (G þ EG) with addition of sucrose in the vitrification process for conservation of caprine morulae in embryo transfer programs. The survival rates in vitro of vitrified-warmed morulae in goats were influenced by their recovery day. Further studies should be conducted to determine if these results are reproducible in vivo embryo transfer on field situations.
publishDate 2022
dc.date.none.fl_str_mv 2022-04-01
2023-01-09T10:16:05Z
2023-01-09T10:16:05Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13847
https://www.sciencedirect.com/science/article/abs/pii/S0093691X22000437
0093-691X
1879-3231
https://doi.org/10.1016/j.theriogenology.2022.02.002
url http://hdl.handle.net/20.500.12123/13847
https://www.sciencedirect.com/science/article/abs/pii/S0093691X22000437
https://doi.org/10.1016/j.theriogenology.2022.02.002
identifier_str_mv 0093-691X
1879-3231
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E5-I107-001/2019-PD-E5-I107-001/AR./Biotecnologías de la reproducción: mejora de la eficiencia reproductiva en especies de interés zootécnico
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Theriogenology 182 : 103-109 (April 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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