Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiot...
- Autores
- Montironi, Ivana D.; Arsaute, Sofía; Roma, Dardo Andrés; Cecchini, María Eugenia; Pinotti, Agustina; Mañas, Fernando; Bessone, Fernando Aníbal; Moreno de LeBlanc, Alejandra de; Alustiza, Fabrisio Eduardo; Bellingeri, Romina; Cariddi, Laura Noelia
- Año de publicación
- 2024
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Early weaning is an important stressor that impairs the piglet´s health, and essential oils appear as promising candidates to improve it instead of antibiotics. The aim of this study was to evaluate the effect of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil (EO and NEO, respectively) on immunological, biochemical and antioxidants parameters as well as on gut microbiota in weaned piglets. EO was extracted by hydrodistillation and nanoencapsulation was performed by high-energy method using Tween 80 and Span 60 as surfactants. EO and NEO were chemically analyzed by gas chromatography-mass spectrometry (GC-MS). The cytotoxic effects of both EO and NEO was evaluated on Caco-2 cell line. For in vivo assay, male weaned piglets (age: 28 days, mean initial body weight: 11.63 ± 0.37 kg) were randomly distributed in six groups of six animals each (n = 6) and received orally EO (10.0 mg/kg/day) or NEO (2.5, 5.0 and 10.0 mg/kg/day), named hereinafter as EO-10, NEO-2.5, NEO-5 and NEO-10, for 30 consecutive days. Animals not treated or treated with surfactants mixture were evaluated as control and vehicle control. Subsequently, histological, hematological and biochemical parameters, cytokines production, oxidative markers, CD4+/CD8+ T cells and gut microbiota were evaluated. GC-MS analysis was similar in both EO and NEO. The NEO was more toxic on Caco-2 cells than EO. Oral supplementation of EO-10 or NEO-10 improved growth performance compared to control group NEO-2.5 or NEO-5 (p < 0.05) groups. NEO-2.5, NEO-5 and NEO-10 did not alter the morpho-physiology of digestive organs and decreased malondialdehyde (MDA) levels in liver compared to control (p < 0.05) or EO-10 groups (p < 0.05, p < 0.01). In addition, NEO-10 showed an increase in CD4+/CD8+ T cells ratio (p < 0.001), and induced the highest serum levels of IL-10 (p < 0.01). Serum triglycerides levels were significantly lower in animals treated with EO-10 or NEO-2.5, NEO-5 and NEO-10 compared to control group (p < 0.001). Gut microbiota analysis showed that NEO-10 favor the development of beneficial intestinal microorganisms to improve parameters related to early weaning of piglets. In conclusion, EO and NEO improved parameters altered by early weaning in piglets however, NEO was safer and powerful. Therefore, NEO should be further studied to be applied in swine health.
EEA Marcos Juárez
Fil: Montironi, Ivana D. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Montironi, Ivana D. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Arsaute, Sofía. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Arsaute, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; Argentina
Fil: Roma, Dardo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; Argentina
Fil: Cecchini, María Eugenia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina.
Fil: Cecchini, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Cecchini, María Eugenia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Pinotti, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; Argentina
Fil: Mañas, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina.
Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; Argentina
Fil: Bessone, Fernando Aníbal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Moreno de LeBlanc, Alejandra de. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Referencia para Lactobacilos (CERELA); Argentina
Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentina
Fil: Bellingeri, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Cariddi, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina - Fuente
- Veterinary Research Communications : 1-18. (Published: 08 March 2024)
- Materia
-
Cerdo
Lechón
Cochinillo Destetado
Nanotecnología
Aceites Esenciales
Minthostachys
Propiedades Antimicrobianas
Inmunología
Swine
Piglets
Weaners
Nanotechnology
Essential Oils
Antimicrobial Properties
Immunology
Minthostachys verticillata - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/17088
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Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned pigletsMontironi, Ivana D.Arsaute, SofíaRoma, Dardo AndrésCecchini, María EugeniaPinotti, AgustinaMañas, FernandoBessone, Fernando AníbalMoreno de LeBlanc, Alejandra deAlustiza, Fabrisio EduardoBellingeri, RominaCariddi, Laura NoeliaCerdoLechónCochinillo DestetadoNanotecnologíaAceites EsencialesMinthostachysPropiedades AntimicrobianasInmunologíaSwinePigletsWeanersNanotechnologyEssential OilsAntimicrobial PropertiesImmunologyMinthostachys verticillataEarly weaning is an important stressor that impairs the piglet´s health, and essential oils appear as promising candidates to improve it instead of antibiotics. The aim of this study was to evaluate the effect of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil (EO and NEO, respectively) on immunological, biochemical and antioxidants parameters as well as on gut microbiota in weaned piglets. EO was extracted by hydrodistillation and nanoencapsulation was performed by high-energy method using Tween 80 and Span 60 as surfactants. EO and NEO were chemically analyzed by gas chromatography-mass spectrometry (GC-MS). The cytotoxic effects of both EO and NEO was evaluated on Caco-2 cell line. For in vivo assay, male weaned piglets (age: 28 days, mean initial body weight: 11.63 ± 0.37 kg) were randomly distributed in six groups of six animals each (n = 6) and received orally EO (10.0 mg/kg/day) or NEO (2.5, 5.0 and 10.0 mg/kg/day), named hereinafter as EO-10, NEO-2.5, NEO-5 and NEO-10, for 30 consecutive days. Animals not treated or treated with surfactants mixture were evaluated as control and vehicle control. Subsequently, histological, hematological and biochemical parameters, cytokines production, oxidative markers, CD4+/CD8+ T cells and gut microbiota were evaluated. GC-MS analysis was similar in both EO and NEO. The NEO was more toxic on Caco-2 cells than EO. Oral supplementation of EO-10 or NEO-10 improved growth performance compared to control group NEO-2.5 or NEO-5 (p < 0.05) groups. NEO-2.5, NEO-5 and NEO-10 did not alter the morpho-physiology of digestive organs and decreased malondialdehyde (MDA) levels in liver compared to control (p < 0.05) or EO-10 groups (p < 0.05, p < 0.01). In addition, NEO-10 showed an increase in CD4+/CD8+ T cells ratio (p < 0.001), and induced the highest serum levels of IL-10 (p < 0.01). Serum triglycerides levels were significantly lower in animals treated with EO-10 or NEO-2.5, NEO-5 and NEO-10 compared to control group (p < 0.001). Gut microbiota analysis showed that NEO-10 favor the development of beneficial intestinal microorganisms to improve parameters related to early weaning of piglets. In conclusion, EO and NEO improved parameters altered by early weaning in piglets however, NEO was safer and powerful. Therefore, NEO should be further studied to be applied in swine health.EEA Marcos JuárezFil: Montironi, Ivana D. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Montironi, Ivana D. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Arsaute, Sofía. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Arsaute, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; ArgentinaFil: Roma, Dardo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; ArgentinaFil: Cecchini, María Eugenia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina.Fil: Cecchini, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Cecchini, María Eugenia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Pinotti, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; ArgentinaFil: Mañas, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina.Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; ArgentinaFil: Bessone, Fernando Aníbal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Moreno de LeBlanc, Alejandra de. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Referencia para Lactobacilos (CERELA); ArgentinaFil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; ArgentinaFil: Bellingeri, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Cariddi, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; ArgentinaSpringer2024-03-18T12:51:07Z2024-03-18T12:51:07Z2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/17088https://link.springer.com/article/10.1007/s11259-024-10347-70165-73801573-7446https://doi.org/10.1007/s11259-024-10347-7Veterinary Research Communications : 1-18. (Published: 08 March 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:25Zoai:localhost:20.500.12123/17088instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:25.557INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets |
title |
Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets |
spellingShingle |
Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets Montironi, Ivana D. Cerdo Lechón Cochinillo Destetado Nanotecnología Aceites Esenciales Minthostachys Propiedades Antimicrobianas Inmunología Swine Piglets Weaners Nanotechnology Essential Oils Antimicrobial Properties Immunology Minthostachys verticillata |
title_short |
Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets |
title_full |
Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets |
title_fullStr |
Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets |
title_full_unstemmed |
Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets |
title_sort |
Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets |
dc.creator.none.fl_str_mv |
Montironi, Ivana D. Arsaute, Sofía Roma, Dardo Andrés Cecchini, María Eugenia Pinotti, Agustina Mañas, Fernando Bessone, Fernando Aníbal Moreno de LeBlanc, Alejandra de Alustiza, Fabrisio Eduardo Bellingeri, Romina Cariddi, Laura Noelia |
author |
Montironi, Ivana D. |
author_facet |
Montironi, Ivana D. Arsaute, Sofía Roma, Dardo Andrés Cecchini, María Eugenia Pinotti, Agustina Mañas, Fernando Bessone, Fernando Aníbal Moreno de LeBlanc, Alejandra de Alustiza, Fabrisio Eduardo Bellingeri, Romina Cariddi, Laura Noelia |
author_role |
author |
author2 |
Arsaute, Sofía Roma, Dardo Andrés Cecchini, María Eugenia Pinotti, Agustina Mañas, Fernando Bessone, Fernando Aníbal Moreno de LeBlanc, Alejandra de Alustiza, Fabrisio Eduardo Bellingeri, Romina Cariddi, Laura Noelia |
author2_role |
author author author author author author author author author author |
dc.subject.none.fl_str_mv |
Cerdo Lechón Cochinillo Destetado Nanotecnología Aceites Esenciales Minthostachys Propiedades Antimicrobianas Inmunología Swine Piglets Weaners Nanotechnology Essential Oils Antimicrobial Properties Immunology Minthostachys verticillata |
topic |
Cerdo Lechón Cochinillo Destetado Nanotecnología Aceites Esenciales Minthostachys Propiedades Antimicrobianas Inmunología Swine Piglets Weaners Nanotechnology Essential Oils Antimicrobial Properties Immunology Minthostachys verticillata |
dc.description.none.fl_txt_mv |
Early weaning is an important stressor that impairs the piglet´s health, and essential oils appear as promising candidates to improve it instead of antibiotics. The aim of this study was to evaluate the effect of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil (EO and NEO, respectively) on immunological, biochemical and antioxidants parameters as well as on gut microbiota in weaned piglets. EO was extracted by hydrodistillation and nanoencapsulation was performed by high-energy method using Tween 80 and Span 60 as surfactants. EO and NEO were chemically analyzed by gas chromatography-mass spectrometry (GC-MS). The cytotoxic effects of both EO and NEO was evaluated on Caco-2 cell line. For in vivo assay, male weaned piglets (age: 28 days, mean initial body weight: 11.63 ± 0.37 kg) were randomly distributed in six groups of six animals each (n = 6) and received orally EO (10.0 mg/kg/day) or NEO (2.5, 5.0 and 10.0 mg/kg/day), named hereinafter as EO-10, NEO-2.5, NEO-5 and NEO-10, for 30 consecutive days. Animals not treated or treated with surfactants mixture were evaluated as control and vehicle control. Subsequently, histological, hematological and biochemical parameters, cytokines production, oxidative markers, CD4+/CD8+ T cells and gut microbiota were evaluated. GC-MS analysis was similar in both EO and NEO. The NEO was more toxic on Caco-2 cells than EO. Oral supplementation of EO-10 or NEO-10 improved growth performance compared to control group NEO-2.5 or NEO-5 (p < 0.05) groups. NEO-2.5, NEO-5 and NEO-10 did not alter the morpho-physiology of digestive organs and decreased malondialdehyde (MDA) levels in liver compared to control (p < 0.05) or EO-10 groups (p < 0.05, p < 0.01). In addition, NEO-10 showed an increase in CD4+/CD8+ T cells ratio (p < 0.001), and induced the highest serum levels of IL-10 (p < 0.01). Serum triglycerides levels were significantly lower in animals treated with EO-10 or NEO-2.5, NEO-5 and NEO-10 compared to control group (p < 0.001). Gut microbiota analysis showed that NEO-10 favor the development of beneficial intestinal microorganisms to improve parameters related to early weaning of piglets. In conclusion, EO and NEO improved parameters altered by early weaning in piglets however, NEO was safer and powerful. Therefore, NEO should be further studied to be applied in swine health. EEA Marcos Juárez Fil: Montironi, Ivana D. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Montironi, Ivana D. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina Fil: Arsaute, Sofía. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Arsaute, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina Fil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; Argentina Fil: Roma, Dardo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; Argentina Fil: Cecchini, María Eugenia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Fil: Cecchini, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina Fil: Cecchini, María Eugenia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina Fil: Pinotti, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; Argentina Fil: Mañas, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina. Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; Argentina Fil: Bessone, Fernando Aníbal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Moreno de LeBlanc, Alejandra de. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Referencia para Lactobacilos (CERELA); Argentina Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina Fil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentina Fil: Bellingeri, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina Fil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina Fil: Cariddi, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina |
description |
Early weaning is an important stressor that impairs the piglet´s health, and essential oils appear as promising candidates to improve it instead of antibiotics. The aim of this study was to evaluate the effect of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil (EO and NEO, respectively) on immunological, biochemical and antioxidants parameters as well as on gut microbiota in weaned piglets. EO was extracted by hydrodistillation and nanoencapsulation was performed by high-energy method using Tween 80 and Span 60 as surfactants. EO and NEO were chemically analyzed by gas chromatography-mass spectrometry (GC-MS). The cytotoxic effects of both EO and NEO was evaluated on Caco-2 cell line. For in vivo assay, male weaned piglets (age: 28 days, mean initial body weight: 11.63 ± 0.37 kg) were randomly distributed in six groups of six animals each (n = 6) and received orally EO (10.0 mg/kg/day) or NEO (2.5, 5.0 and 10.0 mg/kg/day), named hereinafter as EO-10, NEO-2.5, NEO-5 and NEO-10, for 30 consecutive days. Animals not treated or treated with surfactants mixture were evaluated as control and vehicle control. Subsequently, histological, hematological and biochemical parameters, cytokines production, oxidative markers, CD4+/CD8+ T cells and gut microbiota were evaluated. GC-MS analysis was similar in both EO and NEO. The NEO was more toxic on Caco-2 cells than EO. Oral supplementation of EO-10 or NEO-10 improved growth performance compared to control group NEO-2.5 or NEO-5 (p < 0.05) groups. NEO-2.5, NEO-5 and NEO-10 did not alter the morpho-physiology of digestive organs and decreased malondialdehyde (MDA) levels in liver compared to control (p < 0.05) or EO-10 groups (p < 0.05, p < 0.01). In addition, NEO-10 showed an increase in CD4+/CD8+ T cells ratio (p < 0.001), and induced the highest serum levels of IL-10 (p < 0.01). Serum triglycerides levels were significantly lower in animals treated with EO-10 or NEO-2.5, NEO-5 and NEO-10 compared to control group (p < 0.001). Gut microbiota analysis showed that NEO-10 favor the development of beneficial intestinal microorganisms to improve parameters related to early weaning of piglets. In conclusion, EO and NEO improved parameters altered by early weaning in piglets however, NEO was safer and powerful. Therefore, NEO should be further studied to be applied in swine health. |
publishDate |
2024 |
dc.date.none.fl_str_mv |
2024-03-18T12:51:07Z 2024-03-18T12:51:07Z 2024-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/17088 https://link.springer.com/article/10.1007/s11259-024-10347-7 0165-7380 1573-7446 https://doi.org/10.1007/s11259-024-10347-7 |
url |
http://hdl.handle.net/20.500.12123/17088 https://link.springer.com/article/10.1007/s11259-024-10347-7 https://doi.org/10.1007/s11259-024-10347-7 |
identifier_str_mv |
0165-7380 1573-7446 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
restrictedAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
Veterinary Research Communications : 1-18. (Published: 08 March 2024) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1844619185948196864 |
score |
12.559606 |