Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiot...

Autores
Montironi, Ivana D.; Arsaute, Sofía; Roma, Dardo Andrés; Cecchini, María Eugenia; Pinotti, Agustina; Mañas, Fernando; Bessone, Fernando Aníbal; Moreno de LeBlanc, Alejandra de; Alustiza, Fabrisio Eduardo; Bellingeri, Romina; Cariddi, Laura Noelia
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Early weaning is an important stressor that impairs the piglet´s health, and essential oils appear as promising candidates to improve it instead of antibiotics. The aim of this study was to evaluate the effect of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil (EO and NEO, respectively) on immunological, biochemical and antioxidants parameters as well as on gut microbiota in weaned piglets. EO was extracted by hydrodistillation and nanoencapsulation was performed by high-energy method using Tween 80 and Span 60 as surfactants. EO and NEO were chemically analyzed by gas chromatography-mass spectrometry (GC-MS). The cytotoxic effects of both EO and NEO was evaluated on Caco-2 cell line. For in vivo assay, male weaned piglets (age: 28 days, mean initial body weight: 11.63 ± 0.37 kg) were randomly distributed in six groups of six animals each (n = 6) and received orally EO (10.0 mg/kg/day) or NEO (2.5, 5.0 and 10.0 mg/kg/day), named hereinafter as EO-10, NEO-2.5, NEO-5 and NEO-10, for 30 consecutive days. Animals not treated or treated with surfactants mixture were evaluated as control and vehicle control. Subsequently, histological, hematological and biochemical parameters, cytokines production, oxidative markers, CD4+/CD8+ T cells and gut microbiota were evaluated. GC-MS analysis was similar in both EO and NEO. The NEO was more toxic on Caco-2 cells than EO. Oral supplementation of EO-10 or NEO-10 improved growth performance compared to control group NEO-2.5 or NEO-5 (p < 0.05) groups. NEO-2.5, NEO-5 and NEO-10 did not alter the morpho-physiology of digestive organs and decreased malondialdehyde (MDA) levels in liver compared to control (p < 0.05) or EO-10 groups (p < 0.05, p < 0.01). In addition, NEO-10 showed an increase in CD4+/CD8+ T cells ratio (p < 0.001), and induced the highest serum levels of IL-10 (p < 0.01). Serum triglycerides levels were significantly lower in animals treated with EO-10 or NEO-2.5, NEO-5 and NEO-10 compared to control group (p < 0.001). Gut microbiota analysis showed that NEO-10 favor the development of beneficial intestinal microorganisms to improve parameters related to early weaning of piglets. In conclusion, EO and NEO improved parameters altered by early weaning in piglets however, NEO was safer and powerful. Therefore, NEO should be further studied to be applied in swine health.
EEA Marcos Juárez
Fil: Montironi, Ivana D. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Montironi, Ivana D. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Arsaute, Sofía. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Arsaute, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; Argentina
Fil: Roma, Dardo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; Argentina
Fil: Cecchini, María Eugenia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina.
Fil: Cecchini, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Cecchini, María Eugenia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Pinotti, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; Argentina
Fil: Mañas, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina.
Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; Argentina
Fil: Bessone, Fernando Aní­bal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Moreno de LeBlanc, Alejandra de. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Referencia para Lactobacilos (CERELA); Argentina
Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentina
Fil: Bellingeri, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Cariddi, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fuente
Veterinary Research Communications : 1-18. (Published: 08 March 2024)
Materia
Cerdo
Lechón
Cochinillo Destetado
Nanotecnología
Aceites Esenciales
Minthostachys
Propiedades Antimicrobianas
Inmunología
Swine
Piglets
Weaners
Nanotechnology
Essential Oils
Antimicrobial Properties
Immunology
Minthostachys verticillata
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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network_name_str INTA Digital (INTA)
spelling Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned pigletsMontironi, Ivana D.Arsaute, SofíaRoma, Dardo AndrésCecchini, María EugeniaPinotti, AgustinaMañas, FernandoBessone, Fernando AníbalMoreno de LeBlanc, Alejandra deAlustiza, Fabrisio EduardoBellingeri, RominaCariddi, Laura NoeliaCerdoLechónCochinillo DestetadoNanotecnologíaAceites EsencialesMinthostachysPropiedades AntimicrobianasInmunologíaSwinePigletsWeanersNanotechnologyEssential OilsAntimicrobial PropertiesImmunologyMinthostachys verticillataEarly weaning is an important stressor that impairs the piglet´s health, and essential oils appear as promising candidates to improve it instead of antibiotics. The aim of this study was to evaluate the effect of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil (EO and NEO, respectively) on immunological, biochemical and antioxidants parameters as well as on gut microbiota in weaned piglets. EO was extracted by hydrodistillation and nanoencapsulation was performed by high-energy method using Tween 80 and Span 60 as surfactants. EO and NEO were chemically analyzed by gas chromatography-mass spectrometry (GC-MS). The cytotoxic effects of both EO and NEO was evaluated on Caco-2 cell line. For in vivo assay, male weaned piglets (age: 28 days, mean initial body weight: 11.63 ± 0.37 kg) were randomly distributed in six groups of six animals each (n = 6) and received orally EO (10.0 mg/kg/day) or NEO (2.5, 5.0 and 10.0 mg/kg/day), named hereinafter as EO-10, NEO-2.5, NEO-5 and NEO-10, for 30 consecutive days. Animals not treated or treated with surfactants mixture were evaluated as control and vehicle control. Subsequently, histological, hematological and biochemical parameters, cytokines production, oxidative markers, CD4+/CD8+ T cells and gut microbiota were evaluated. GC-MS analysis was similar in both EO and NEO. The NEO was more toxic on Caco-2 cells than EO. Oral supplementation of EO-10 or NEO-10 improved growth performance compared to control group NEO-2.5 or NEO-5 (p < 0.05) groups. NEO-2.5, NEO-5 and NEO-10 did not alter the morpho-physiology of digestive organs and decreased malondialdehyde (MDA) levels in liver compared to control (p < 0.05) or EO-10 groups (p < 0.05, p < 0.01). In addition, NEO-10 showed an increase in CD4+/CD8+ T cells ratio (p < 0.001), and induced the highest serum levels of IL-10 (p < 0.01). Serum triglycerides levels were significantly lower in animals treated with EO-10 or NEO-2.5, NEO-5 and NEO-10 compared to control group (p < 0.001). Gut microbiota analysis showed that NEO-10 favor the development of beneficial intestinal microorganisms to improve parameters related to early weaning of piglets. In conclusion, EO and NEO improved parameters altered by early weaning in piglets however, NEO was safer and powerful. Therefore, NEO should be further studied to be applied in swine health.EEA Marcos JuárezFil: Montironi, Ivana D. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Montironi, Ivana D. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Arsaute, Sofía. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Arsaute, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; ArgentinaFil: Roma, Dardo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; ArgentinaFil: Cecchini, María Eugenia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina.Fil: Cecchini, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Cecchini, María Eugenia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Pinotti, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; ArgentinaFil: Mañas, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina.Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; ArgentinaFil: Bessone, Fernando Aní­bal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Moreno de LeBlanc, Alejandra de. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Referencia para Lactobacilos (CERELA); ArgentinaFil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; ArgentinaFil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; ArgentinaFil: Bellingeri, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; ArgentinaFil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; ArgentinaFil: Cariddi, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; ArgentinaFil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; ArgentinaSpringer2024-03-18T12:51:07Z2024-03-18T12:51:07Z2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/17088https://link.springer.com/article/10.1007/s11259-024-10347-70165-73801573-7446https://doi.org/10.1007/s11259-024-10347-7Veterinary Research Communications : 1-18. (Published: 08 March 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:46:25Zoai:localhost:20.500.12123/17088instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:46:25.557INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets
title Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets
spellingShingle Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets
Montironi, Ivana D.
Cerdo
Lechón
Cochinillo Destetado
Nanotecnología
Aceites Esenciales
Minthostachys
Propiedades Antimicrobianas
Inmunología
Swine
Piglets
Weaners
Nanotechnology
Essential Oils
Antimicrobial Properties
Immunology
Minthostachys verticillata
title_short Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets
title_full Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets
title_fullStr Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets
title_full_unstemmed Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets
title_sort Evaluation of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil on immunological, biochemical and antioxidants parameters and gut microbiota in weaned piglets
dc.creator.none.fl_str_mv Montironi, Ivana D.
Arsaute, Sofía
Roma, Dardo Andrés
Cecchini, María Eugenia
Pinotti, Agustina
Mañas, Fernando
Bessone, Fernando Aníbal
Moreno de LeBlanc, Alejandra de
Alustiza, Fabrisio Eduardo
Bellingeri, Romina
Cariddi, Laura Noelia
author Montironi, Ivana D.
author_facet Montironi, Ivana D.
Arsaute, Sofía
Roma, Dardo Andrés
Cecchini, María Eugenia
Pinotti, Agustina
Mañas, Fernando
Bessone, Fernando Aníbal
Moreno de LeBlanc, Alejandra de
Alustiza, Fabrisio Eduardo
Bellingeri, Romina
Cariddi, Laura Noelia
author_role author
author2 Arsaute, Sofía
Roma, Dardo Andrés
Cecchini, María Eugenia
Pinotti, Agustina
Mañas, Fernando
Bessone, Fernando Aníbal
Moreno de LeBlanc, Alejandra de
Alustiza, Fabrisio Eduardo
Bellingeri, Romina
Cariddi, Laura Noelia
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cerdo
Lechón
Cochinillo Destetado
Nanotecnología
Aceites Esenciales
Minthostachys
Propiedades Antimicrobianas
Inmunología
Swine
Piglets
Weaners
Nanotechnology
Essential Oils
Antimicrobial Properties
Immunology
Minthostachys verticillata
topic Cerdo
Lechón
Cochinillo Destetado
Nanotecnología
Aceites Esenciales
Minthostachys
Propiedades Antimicrobianas
Inmunología
Swine
Piglets
Weaners
Nanotechnology
Essential Oils
Antimicrobial Properties
Immunology
Minthostachys verticillata
dc.description.none.fl_txt_mv Early weaning is an important stressor that impairs the piglet´s health, and essential oils appear as promising candidates to improve it instead of antibiotics. The aim of this study was to evaluate the effect of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil (EO and NEO, respectively) on immunological, biochemical and antioxidants parameters as well as on gut microbiota in weaned piglets. EO was extracted by hydrodistillation and nanoencapsulation was performed by high-energy method using Tween 80 and Span 60 as surfactants. EO and NEO were chemically analyzed by gas chromatography-mass spectrometry (GC-MS). The cytotoxic effects of both EO and NEO was evaluated on Caco-2 cell line. For in vivo assay, male weaned piglets (age: 28 days, mean initial body weight: 11.63 ± 0.37 kg) were randomly distributed in six groups of six animals each (n = 6) and received orally EO (10.0 mg/kg/day) or NEO (2.5, 5.0 and 10.0 mg/kg/day), named hereinafter as EO-10, NEO-2.5, NEO-5 and NEO-10, for 30 consecutive days. Animals not treated or treated with surfactants mixture were evaluated as control and vehicle control. Subsequently, histological, hematological and biochemical parameters, cytokines production, oxidative markers, CD4+/CD8+ T cells and gut microbiota were evaluated. GC-MS analysis was similar in both EO and NEO. The NEO was more toxic on Caco-2 cells than EO. Oral supplementation of EO-10 or NEO-10 improved growth performance compared to control group NEO-2.5 or NEO-5 (p < 0.05) groups. NEO-2.5, NEO-5 and NEO-10 did not alter the morpho-physiology of digestive organs and decreased malondialdehyde (MDA) levels in liver compared to control (p < 0.05) or EO-10 groups (p < 0.05, p < 0.01). In addition, NEO-10 showed an increase in CD4+/CD8+ T cells ratio (p < 0.001), and induced the highest serum levels of IL-10 (p < 0.01). Serum triglycerides levels were significantly lower in animals treated with EO-10 or NEO-2.5, NEO-5 and NEO-10 compared to control group (p < 0.001). Gut microbiota analysis showed that NEO-10 favor the development of beneficial intestinal microorganisms to improve parameters related to early weaning of piglets. In conclusion, EO and NEO improved parameters altered by early weaning in piglets however, NEO was safer and powerful. Therefore, NEO should be further studied to be applied in swine health.
EEA Marcos Juárez
Fil: Montironi, Ivana D. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Montironi, Ivana D. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Arsaute, Sofía. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Arsaute, Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Roma, Dardo Andrés. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; Argentina
Fil: Roma, Dardo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; Argentina
Fil: Cecchini, María Eugenia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina.
Fil: Cecchini, María Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Cecchini, María Eugenia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Pinotti, Agustina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Cátedra de Farmacología; Argentina
Fil: Mañas, Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias; Argentina.
Fil: Mañas, Fernando. Universidad Nacional de Río Cuarto. Instituto de Ciencias Veterinarias; Argentina
Fil: Bessone, Fernando Aní­bal. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Moreno de LeBlanc, Alejandra de. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Referencia para Lactobacilos (CERELA); Argentina
Fil: Alustiza, Fabrisio Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Marcos Juárez; Argentina
Fil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria. Departamento de Anatomía Animal. Laboratorio de Biotecnología Animal; Argentina
Fil: Bellingeri, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Bellingeri, Romina. Universidad Nacional de Río Cuarto. Instituto de Investigaciones en Tecnologías Energéticas y Materiales Avanzados; Argentina
Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Físico-Químicas y Naturales. Departamento de Microbiología e Inmunología; Argentina
Fil: Cariddi, Noelia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biotecnología Ambiental y Salud; Argentina
Fil: Cariddi, Noelia. Universidad Nacional de Río Cuarto. Instituto de Biotecnología Ambiental y Salud; Argentina
description Early weaning is an important stressor that impairs the piglet´s health, and essential oils appear as promising candidates to improve it instead of antibiotics. The aim of this study was to evaluate the effect of oral supplementation of free and nanoencapsulated Minthostachys verticillata essential oil (EO and NEO, respectively) on immunological, biochemical and antioxidants parameters as well as on gut microbiota in weaned piglets. EO was extracted by hydrodistillation and nanoencapsulation was performed by high-energy method using Tween 80 and Span 60 as surfactants. EO and NEO were chemically analyzed by gas chromatography-mass spectrometry (GC-MS). The cytotoxic effects of both EO and NEO was evaluated on Caco-2 cell line. For in vivo assay, male weaned piglets (age: 28 days, mean initial body weight: 11.63 ± 0.37 kg) were randomly distributed in six groups of six animals each (n = 6) and received orally EO (10.0 mg/kg/day) or NEO (2.5, 5.0 and 10.0 mg/kg/day), named hereinafter as EO-10, NEO-2.5, NEO-5 and NEO-10, for 30 consecutive days. Animals not treated or treated with surfactants mixture were evaluated as control and vehicle control. Subsequently, histological, hematological and biochemical parameters, cytokines production, oxidative markers, CD4+/CD8+ T cells and gut microbiota were evaluated. GC-MS analysis was similar in both EO and NEO. The NEO was more toxic on Caco-2 cells than EO. Oral supplementation of EO-10 or NEO-10 improved growth performance compared to control group NEO-2.5 or NEO-5 (p < 0.05) groups. NEO-2.5, NEO-5 and NEO-10 did not alter the morpho-physiology of digestive organs and decreased malondialdehyde (MDA) levels in liver compared to control (p < 0.05) or EO-10 groups (p < 0.05, p < 0.01). In addition, NEO-10 showed an increase in CD4+/CD8+ T cells ratio (p < 0.001), and induced the highest serum levels of IL-10 (p < 0.01). Serum triglycerides levels were significantly lower in animals treated with EO-10 or NEO-2.5, NEO-5 and NEO-10 compared to control group (p < 0.001). Gut microbiota analysis showed that NEO-10 favor the development of beneficial intestinal microorganisms to improve parameters related to early weaning of piglets. In conclusion, EO and NEO improved parameters altered by early weaning in piglets however, NEO was safer and powerful. Therefore, NEO should be further studied to be applied in swine health.
publishDate 2024
dc.date.none.fl_str_mv 2024-03-18T12:51:07Z
2024-03-18T12:51:07Z
2024-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/17088
https://link.springer.com/article/10.1007/s11259-024-10347-7
0165-7380
1573-7446
https://doi.org/10.1007/s11259-024-10347-7
url http://hdl.handle.net/20.500.12123/17088
https://link.springer.com/article/10.1007/s11259-024-10347-7
https://doi.org/10.1007/s11259-024-10347-7
identifier_str_mv 0165-7380
1573-7446
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Veterinary Research Communications : 1-18. (Published: 08 March 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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