Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows

Autores
Petersen, Marcos Iván; Suarez Archilla, Guillermo; Miretti, Marcos Mateo; Trono, Karina Gabriela; Carignano, Hugo Adrian
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine leukemia virus (BLV) is a retrovirus that infects cattle, causing bovine enzootic leukosis, a chronic disease characterized by the proliferation of infected B cells. BLV proviral load (PVL) is a key determinant of disease progression and transmission risk. Cattle can exhibit distinct phenotypes of low PVL (LPVL) or high PVL (HPVL), which remain stable throughout their lifetime. Differential expression analysis revealed 1,908 differentially expressed genes (DEGs) between HPVL and LPVL animals, including 774 downregulated (DReg) and 1,134 upregulated (UReg) genes. Functional enrichment analysis revealed that DReg genes were associated primarily with immune response pathways. Conversely, the UReg genes were enriched in processes related to cell cycle regulation, mitotic division, and DNA biosynthesis. Protein–protein interaction analysis revealed six highly interconnected clusters. Interestingly, a cluster was enriched for sphingolipid metabolism, a process critical to enveloped virus infection and immune receptor signaling. These findings provide valuable insights into the molecular mechanisms of BLV infection, suggesting potential markers for disease monitoring and targets for therapeutic intervention.
Instituto de Virología
Fil: Petersen, Marcos Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Petersen, Marcos Iván. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Miretti, Marcos Mateo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Miretti, Marcos Mateo. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical. Grupo de Investigación en Genética Aplicada; Argentina
Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Trono, Karina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Carignano, Hugo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Frontiers in Veterinary Science 12 : 1550646 (April 2025)
Materia
Bovine Leukemia Virus
Gene Expression
RNA Sequence
Transcriptome
Cows
Immune Response
Proviruses
Virus Leucemia Bovina
Expresión Génica
Secuencia de ARN
Transcriptoma
Vaca
Respuesta Inmunológica
Provirus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23157
Acceder
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oai_identifier_str oai:localhost:20.500.12123/23157
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network_name_str INTA Digital (INTA)
spelling Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cowsPetersen, Marcos IvánSuarez Archilla, GuillermoMiretti, Marcos MateoTrono, Karina GabrielaCarignano, Hugo AdrianBovine Leukemia VirusGene ExpressionRNA SequenceTranscriptomeCowsImmune ResponseProvirusesVirus Leucemia BovinaExpresión GénicaSecuencia de ARNTranscriptomaVacaRespuesta InmunológicaProvirusBovine leukemia virus (BLV) is a retrovirus that infects cattle, causing bovine enzootic leukosis, a chronic disease characterized by the proliferation of infected B cells. BLV proviral load (PVL) is a key determinant of disease progression and transmission risk. Cattle can exhibit distinct phenotypes of low PVL (LPVL) or high PVL (HPVL), which remain stable throughout their lifetime. Differential expression analysis revealed 1,908 differentially expressed genes (DEGs) between HPVL and LPVL animals, including 774 downregulated (DReg) and 1,134 upregulated (UReg) genes. Functional enrichment analysis revealed that DReg genes were associated primarily with immune response pathways. Conversely, the UReg genes were enriched in processes related to cell cycle regulation, mitotic division, and DNA biosynthesis. Protein–protein interaction analysis revealed six highly interconnected clusters. Interestingly, a cluster was enriched for sphingolipid metabolism, a process critical to enveloped virus infection and immune receptor signaling. These findings provide valuable insights into the molecular mechanisms of BLV infection, suggesting potential markers for disease monitoring and targets for therapeutic intervention.Instituto de VirologíaFil: Petersen, Marcos Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Petersen, Marcos Iván. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Miretti, Marcos Mateo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Miretti, Marcos Mateo. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical. Grupo de Investigación en Genética Aplicada; ArgentinaFil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Trono, Karina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Carignano, Hugo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFrontiers Media2025-07-24T13:40:03Z2025-07-24T13:40:03Z2025-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23157https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1550646/full2297-1769https://doi.org/10.3389/fvets.2025.1550646Frontiers in Veterinary Science 12 : 1550646 (April 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Saludinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:51:12Zoai:localhost:20.500.12123/23157instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:51:12.599INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows
title Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows
spellingShingle Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows
Petersen, Marcos Iván
Bovine Leukemia Virus
Gene Expression
RNA Sequence
Transcriptome
Cows
Immune Response
Proviruses
Virus Leucemia Bovina
Expresión Génica
Secuencia de ARN
Transcriptoma
Vaca
Respuesta Inmunológica
Provirus
title_short Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows
title_full Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows
title_fullStr Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows
title_full_unstemmed Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows
title_sort Whole-transcriptome analysis of BLV-infected cows reveals downregulation of immune response genes in high proviral loads cows
dc.creator.none.fl_str_mv Petersen, Marcos Iván
Suarez Archilla, Guillermo
Miretti, Marcos Mateo
Trono, Karina Gabriela
Carignano, Hugo Adrian
author Petersen, Marcos Iván
author_facet Petersen, Marcos Iván
Suarez Archilla, Guillermo
Miretti, Marcos Mateo
Trono, Karina Gabriela
Carignano, Hugo Adrian
author_role author
author2 Suarez Archilla, Guillermo
Miretti, Marcos Mateo
Trono, Karina Gabriela
Carignano, Hugo Adrian
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bovine Leukemia Virus
Gene Expression
RNA Sequence
Transcriptome
Cows
Immune Response
Proviruses
Virus Leucemia Bovina
Expresión Génica
Secuencia de ARN
Transcriptoma
Vaca
Respuesta Inmunológica
Provirus
topic Bovine Leukemia Virus
Gene Expression
RNA Sequence
Transcriptome
Cows
Immune Response
Proviruses
Virus Leucemia Bovina
Expresión Génica
Secuencia de ARN
Transcriptoma
Vaca
Respuesta Inmunológica
Provirus
dc.description.none.fl_txt_mv Bovine leukemia virus (BLV) is a retrovirus that infects cattle, causing bovine enzootic leukosis, a chronic disease characterized by the proliferation of infected B cells. BLV proviral load (PVL) is a key determinant of disease progression and transmission risk. Cattle can exhibit distinct phenotypes of low PVL (LPVL) or high PVL (HPVL), which remain stable throughout their lifetime. Differential expression analysis revealed 1,908 differentially expressed genes (DEGs) between HPVL and LPVL animals, including 774 downregulated (DReg) and 1,134 upregulated (UReg) genes. Functional enrichment analysis revealed that DReg genes were associated primarily with immune response pathways. Conversely, the UReg genes were enriched in processes related to cell cycle regulation, mitotic division, and DNA biosynthesis. Protein–protein interaction analysis revealed six highly interconnected clusters. Interestingly, a cluster was enriched for sphingolipid metabolism, a process critical to enveloped virus infection and immune receptor signaling. These findings provide valuable insights into the molecular mechanisms of BLV infection, suggesting potential markers for disease monitoring and targets for therapeutic intervention.
Instituto de Virología
Fil: Petersen, Marcos Iván. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Petersen, Marcos Iván. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Suarez Archilla, Guillermo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Miretti, Marcos Mateo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Miretti, Marcos Mateo. Universidad Nacional de Misiones. Facultad de Ciencias Exactas, Químicas y Naturales. Instituto de Biología Subtropical. Grupo de Investigación en Genética Aplicada; Argentina
Fil: Trono, Karina Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Trono, Karina Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Carignano, Hugo Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Carignano, Hugo Adrian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Bovine leukemia virus (BLV) is a retrovirus that infects cattle, causing bovine enzootic leukosis, a chronic disease characterized by the proliferation of infected B cells. BLV proviral load (PVL) is a key determinant of disease progression and transmission risk. Cattle can exhibit distinct phenotypes of low PVL (LPVL) or high PVL (HPVL), which remain stable throughout their lifetime. Differential expression analysis revealed 1,908 differentially expressed genes (DEGs) between HPVL and LPVL animals, including 774 downregulated (DReg) and 1,134 upregulated (UReg) genes. Functional enrichment analysis revealed that DReg genes were associated primarily with immune response pathways. Conversely, the UReg genes were enriched in processes related to cell cycle regulation, mitotic division, and DNA biosynthesis. Protein–protein interaction analysis revealed six highly interconnected clusters. Interestingly, a cluster was enriched for sphingolipid metabolism, a process critical to enveloped virus infection and immune receptor signaling. These findings provide valuable insights into the molecular mechanisms of BLV infection, suggesting potential markers for disease monitoring and targets for therapeutic intervention.
publishDate 2025
dc.date.none.fl_str_mv 2025-07-24T13:40:03Z
2025-07-24T13:40:03Z
2025-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23157
https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1550646/full
2297-1769
https://doi.org/10.3389/fvets.2025.1550646
url http://hdl.handle.net/20.500.12123/23157
https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2025.1550646/full
https://doi.org/10.3389/fvets.2025.1550646
identifier_str_mv 2297-1769
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I113, Herramientas de estudio de la Patogenia e Inmunidad en agentes infecciosos y tóxicos que aporten a la sustentabilidad de la producción pecuaria en el marco de Una Salud
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Frontiers in Veterinary Science 12 : 1550646 (April 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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