Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010

Autores
Bok, Marina; Miño, Samuel; Rodriguez, Daniela; Badaracco, Alejandra; Nuñes, I.; Souza, Sibele P.; Bilbao, G.; Louge Uriarte, Enrique Leopoldo; Galarza, Roxana Ivon; Vega, Celina Guadalupe; Odeon, Anselmo Carlos; Saif, LInda J.; Parreño, Gladys Viviana
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bovine coronavirus (BCoV) is an important viral pathogen associated with neonatal calf diarrhea. Our aim was to investigate the incidence of BCoV in diarrhea outbreaks in beef and dairy herds from Argentina during 1994–2010. A total of 5.365 fecal samples from diarrheic calves were screened for BCoV diagnosis by ELISA. The virus was detected in 1.71% (92/5365) of the samples corresponding to 5.95% (63/1058) of the diarrhea cases in 239 beef and 324 dairy farms. The detection rate of BCoV was significantly higher in dairy than in beef herds: 12.13% (29/239) vs. 4.32% (14/324) respectively. Phylogenetic analysis of the hypervariable S1 region of seven representative samples (from different husbandry systems, farm locations and years of sampling) indicated that BCoV strains circulating in Argentinean beef and dairy herds formed a cluster distinct from other geographical regions. Interestingly, Argentinean strains are distantly related (at both the nucleotide and amino acid levels) with the Mebus historic reference BCoV strain included in the vaccines currently available in Argentina. However, Mebus-induced antibodies were capable of neutralizing the BCoV Arg95, a field strain adapted to grow in vitro, and vice versa, indicating that both strains belong to the same CoV serotype reported in cattle. This work represents the first large survey describing BCoV circulation in Argentinean cattle
Instituto de Virología
Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Chinese Academy of Agriculture Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Rodriguez, Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Nuñes, I. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
Fil: Souza, Sibele P. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
Fil: Bilbao, G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Sanidad Animal; Argentina
Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional del Centro de la provincia de Buenos Aires. Facultad Ciencias Veterinarias; Argentina
Fil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Odeon, Anselmo Carlos. Estación Experimental Agropecuaria Balcarce. Laboratorio de Sanidad Animal; Argentina
Fil: Saif, LInda J. Ohio State University. Food Animal Health Research Program; Estados Unidos
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fuente
Veterinary microbiology 181 (3–4) : 221-229. (31 December 2015)
Materia
Coronavirus
Ganado Bovino
Diarrea Viral Bovina
Cattle
Bovine Viral Diarrhoea
Argentina
Neonatal Calf Diarrhea
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4591
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4591
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010Bok, MarinaMiño, SamuelRodriguez, DanielaBadaracco, AlejandraNuñes, I.Souza, Sibele P.Bilbao, G.Louge Uriarte, Enrique LeopoldoGalarza, Roxana IvonVega, Celina GuadalupeOdeon, Anselmo CarlosSaif, LInda J.Parreño, Gladys VivianaCoronavirusGanado BovinoDiarrea Viral BovinaCattleBovine Viral DiarrhoeaArgentinaNeonatal Calf DiarrheaBovine coronavirus (BCoV) is an important viral pathogen associated with neonatal calf diarrhea. Our aim was to investigate the incidence of BCoV in diarrhea outbreaks in beef and dairy herds from Argentina during 1994–2010. A total of 5.365 fecal samples from diarrheic calves were screened for BCoV diagnosis by ELISA. The virus was detected in 1.71% (92/5365) of the samples corresponding to 5.95% (63/1058) of the diarrhea cases in 239 beef and 324 dairy farms. The detection rate of BCoV was significantly higher in dairy than in beef herds: 12.13% (29/239) vs. 4.32% (14/324) respectively. Phylogenetic analysis of the hypervariable S1 region of seven representative samples (from different husbandry systems, farm locations and years of sampling) indicated that BCoV strains circulating in Argentinean beef and dairy herds formed a cluster distinct from other geographical regions. Interestingly, Argentinean strains are distantly related (at both the nucleotide and amino acid levels) with the Mebus historic reference BCoV strain included in the vaccines currently available in Argentina. However, Mebus-induced antibodies were capable of neutralizing the BCoV Arg95, a field strain adapted to grow in vitro, and vice versa, indicating that both strains belong to the same CoV serotype reported in cattle. This work represents the first large survey describing BCoV circulation in Argentinean cattleInstituto de VirologíaFil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Chinese Academy of Agriculture Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; ChinaFil: Rodriguez, Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Nuñes, I. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; BrasilFil: Souza, Sibele P. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; BrasilFil: Bilbao, G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Sanidad Animal; ArgentinaFil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional del Centro de la provincia de Buenos Aires. Facultad Ciencias Veterinarias; ArgentinaFil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Odeon, Anselmo Carlos. Estación Experimental Agropecuaria Balcarce. Laboratorio de Sanidad Animal; ArgentinaFil: Saif, LInda J. Ohio State University. Food Animal Health Research Program; Estados UnidosFil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaElsevier2019-03-13T14:46:00Z2019-03-13T14:46:00Z2015-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4591https://www.sciencedirect.com/science/article/pii/S0378113515300523?via%3Dihub0378-1135https://doi.org/10.1016/j.vetmic.2015.10.017Veterinary microbiology 181 (3–4) : 221-229. (31 December 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-18T10:07:29Zoai:localhost:20.500.12123/4591instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-18 10:07:29.72INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
title Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
spellingShingle Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
Bok, Marina
Coronavirus
Ganado Bovino
Diarrea Viral Bovina
Cattle
Bovine Viral Diarrhoea
Argentina
Neonatal Calf Diarrhea
title_short Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
title_full Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
title_fullStr Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
title_full_unstemmed Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
title_sort Molecular and antigenic characterization of bovine Coronavirus circulating in Argentinean cattle during 1994–2010
dc.creator.none.fl_str_mv Bok, Marina
Miño, Samuel
Rodriguez, Daniela
Badaracco, Alejandra
Nuñes, I.
Souza, Sibele P.
Bilbao, G.
Louge Uriarte, Enrique Leopoldo
Galarza, Roxana Ivon
Vega, Celina Guadalupe
Odeon, Anselmo Carlos
Saif, LInda J.
Parreño, Gladys Viviana
author Bok, Marina
author_facet Bok, Marina
Miño, Samuel
Rodriguez, Daniela
Badaracco, Alejandra
Nuñes, I.
Souza, Sibele P.
Bilbao, G.
Louge Uriarte, Enrique Leopoldo
Galarza, Roxana Ivon
Vega, Celina Guadalupe
Odeon, Anselmo Carlos
Saif, LInda J.
Parreño, Gladys Viviana
author_role author
author2 Miño, Samuel
Rodriguez, Daniela
Badaracco, Alejandra
Nuñes, I.
Souza, Sibele P.
Bilbao, G.
Louge Uriarte, Enrique Leopoldo
Galarza, Roxana Ivon
Vega, Celina Guadalupe
Odeon, Anselmo Carlos
Saif, LInda J.
Parreño, Gladys Viviana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Coronavirus
Ganado Bovino
Diarrea Viral Bovina
Cattle
Bovine Viral Diarrhoea
Argentina
Neonatal Calf Diarrhea
topic Coronavirus
Ganado Bovino
Diarrea Viral Bovina
Cattle
Bovine Viral Diarrhoea
Argentina
Neonatal Calf Diarrhea
dc.description.none.fl_txt_mv Bovine coronavirus (BCoV) is an important viral pathogen associated with neonatal calf diarrhea. Our aim was to investigate the incidence of BCoV in diarrhea outbreaks in beef and dairy herds from Argentina during 1994–2010. A total of 5.365 fecal samples from diarrheic calves were screened for BCoV diagnosis by ELISA. The virus was detected in 1.71% (92/5365) of the samples corresponding to 5.95% (63/1058) of the diarrhea cases in 239 beef and 324 dairy farms. The detection rate of BCoV was significantly higher in dairy than in beef herds: 12.13% (29/239) vs. 4.32% (14/324) respectively. Phylogenetic analysis of the hypervariable S1 region of seven representative samples (from different husbandry systems, farm locations and years of sampling) indicated that BCoV strains circulating in Argentinean beef and dairy herds formed a cluster distinct from other geographical regions. Interestingly, Argentinean strains are distantly related (at both the nucleotide and amino acid levels) with the Mebus historic reference BCoV strain included in the vaccines currently available in Argentina. However, Mebus-induced antibodies were capable of neutralizing the BCoV Arg95, a field strain adapted to grow in vitro, and vice versa, indicating that both strains belong to the same CoV serotype reported in cattle. This work represents the first large survey describing BCoV circulation in Argentinean cattle
Instituto de Virología
Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Miño, Samuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Chinese Academy of Agriculture Sciences. Harbin Veterinary Research Institute. State Key Laboratory of Veterinary Biotechnology; China
Fil: Rodriguez, Daniela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Nuñes, I. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
Fil: Souza, Sibele P. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
Fil: Bilbao, G. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Laboratorio de Sanidad Animal; Argentina
Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional del Centro de la provincia de Buenos Aires. Facultad Ciencias Veterinarias; Argentina
Fil: Galarza, Roxana Ivon. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Odeon, Anselmo Carlos. Estación Experimental Agropecuaria Balcarce. Laboratorio de Sanidad Animal; Argentina
Fil: Saif, LInda J. Ohio State University. Food Animal Health Research Program; Estados Unidos
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
description Bovine coronavirus (BCoV) is an important viral pathogen associated with neonatal calf diarrhea. Our aim was to investigate the incidence of BCoV in diarrhea outbreaks in beef and dairy herds from Argentina during 1994–2010. A total of 5.365 fecal samples from diarrheic calves were screened for BCoV diagnosis by ELISA. The virus was detected in 1.71% (92/5365) of the samples corresponding to 5.95% (63/1058) of the diarrhea cases in 239 beef and 324 dairy farms. The detection rate of BCoV was significantly higher in dairy than in beef herds: 12.13% (29/239) vs. 4.32% (14/324) respectively. Phylogenetic analysis of the hypervariable S1 region of seven representative samples (from different husbandry systems, farm locations and years of sampling) indicated that BCoV strains circulating in Argentinean beef and dairy herds formed a cluster distinct from other geographical regions. Interestingly, Argentinean strains are distantly related (at both the nucleotide and amino acid levels) with the Mebus historic reference BCoV strain included in the vaccines currently available in Argentina. However, Mebus-induced antibodies were capable of neutralizing the BCoV Arg95, a field strain adapted to grow in vitro, and vice versa, indicating that both strains belong to the same CoV serotype reported in cattle. This work represents the first large survey describing BCoV circulation in Argentinean cattle
publishDate 2015
dc.date.none.fl_str_mv 2015-12
2019-03-13T14:46:00Z
2019-03-13T14:46:00Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4591
https://www.sciencedirect.com/science/article/pii/S0378113515300523?via%3Dihub
0378-1135
https://doi.org/10.1016/j.vetmic.2015.10.017
url http://hdl.handle.net/20.500.12123/4591
https://www.sciencedirect.com/science/article/pii/S0378113515300523?via%3Dihub
https://doi.org/10.1016/j.vetmic.2015.10.017
identifier_str_mv 0378-1135
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary microbiology 181 (3–4) : 221-229. (31 December 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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