Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth
- Autores
- Maurizio, Estefanía; Trangoni, Marcos David; Rossi, Ursula; Dunleavy, Mariana Victoria; Colato, Claudio; Rossetti, Carlos Alberto
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Caprine brucellosis is a chronic, world-wide distributed disease which causes reproductive failure in goats and Brucella melitensis, its causative agent, bears a great zoonotic potential. There is evidence suggesting that some cattle and pigs have an innate ability to resist Brucella infection, but this has not yet been investigated in goats. In this study, we compared caprine macrophages that exhibit extreme restriction and permissiveness to B. melitensis’ intracellular growth in vitro. Monocyte derived macrophages (MDMs) from 110 female goats were cultured and challenged in vitro with B. melitensis 16 M. After initial screening, 18 donor goats were selected based on their macrophages ability to restrict or allow bacterial intracellular growth and some elements of humoral and cellular immunity were studied in depth. MDMs that were able to restrict the pathogen’s intracellular growth showed enhanced bacterial internalization, although there were no differences between groups in the production of reactive oxygen and nitrogen intermediates following 48 h treatment with heat-killed B. melitensis. Moreover, there were no differences between groups in the level of antibodies reacting with keyhole limpet hemocyanin (natural antibodies, NAbs) or with Brucella LPS antigens (cross-reacting antibodies, CrAbs), although a strong positive correlation between individual levels of IgM NAbs and IgM CrAbs was detected. Altogether, these results represent an initial step in understanding innate primary host response to B. melitensis, and deciphering which mechanisms may determine a successful outcome of the infection in goats.
Instituto de Patobiología
Fil: Maurizio, Estefania. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Maurizio, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Argentina
Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rossi, Ursula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Rossi, Ursula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dunleavy, Mariana Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Dunleavy, Mariana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Colato, Claudio. Cabaña Nuevo Milenium; Argentina
Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Rossetti, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Veterinary Immunology and Immunopathology 234 : 110223 (Abril 2021)
- Materia
-
Caprinae
Brucelosis
Macrofagos
Anticuerpos
Caprinos
Brucella melitensis
Fenotipos
Trastornos respiratorios
Brucellosis
Macrophages
Antibodies
Goats
Phenotypes
Respiratory Disorders - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/9079
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Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growthMaurizio, EstefaníaTrangoni, Marcos DavidRossi, UrsulaDunleavy, Mariana VictoriaColato, ClaudioRossetti, Carlos AlbertoCaprinaeBrucelosisMacrofagosAnticuerposCaprinosBrucella melitensisFenotiposTrastornos respiratoriosBrucellosisMacrophagesAntibodiesGoatsPhenotypesRespiratory DisordersCaprine brucellosis is a chronic, world-wide distributed disease which causes reproductive failure in goats and Brucella melitensis, its causative agent, bears a great zoonotic potential. There is evidence suggesting that some cattle and pigs have an innate ability to resist Brucella infection, but this has not yet been investigated in goats. In this study, we compared caprine macrophages that exhibit extreme restriction and permissiveness to B. melitensis’ intracellular growth in vitro. Monocyte derived macrophages (MDMs) from 110 female goats were cultured and challenged in vitro with B. melitensis 16 M. After initial screening, 18 donor goats were selected based on their macrophages ability to restrict or allow bacterial intracellular growth and some elements of humoral and cellular immunity were studied in depth. MDMs that were able to restrict the pathogen’s intracellular growth showed enhanced bacterial internalization, although there were no differences between groups in the production of reactive oxygen and nitrogen intermediates following 48 h treatment with heat-killed B. melitensis. Moreover, there were no differences between groups in the level of antibodies reacting with keyhole limpet hemocyanin (natural antibodies, NAbs) or with Brucella LPS antigens (cross-reacting antibodies, CrAbs), although a strong positive correlation between individual levels of IgM NAbs and IgM CrAbs was detected. Altogether, these results represent an initial step in understanding innate primary host response to B. melitensis, and deciphering which mechanisms may determine a successful outcome of the infection in goats.Instituto de PatobiologíaFil: Maurizio, Estefania. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Maurizio, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. ArgentinaFil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rossi, Ursula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Rossi, Ursula. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dunleavy, Mariana Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Dunleavy, Mariana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Colato, Claudio. Cabaña Nuevo Milenium; ArgentinaFil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Rossetti, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2021-04-13T15:33:04Z2021-04-13T15:33:04Z2021-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/9079https://www.sciencedirect.com/science/article/abs/pii/S01652427210004160165-2427https://doi.org/10.1016/j.vetimm.2021.110223Veterinary Immunology and Immunopathology 234 : 110223 (Abril 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional.info:eu-repo/semantics/restrictedAccess2025-09-29T13:45:10Zoai:localhost:20.500.12123/9079instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:11.2INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth |
title |
Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth |
spellingShingle |
Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth Maurizio, Estefanía Caprinae Brucelosis Macrofagos Anticuerpos Caprinos Brucella melitensis Fenotipos Trastornos respiratorios Brucellosis Macrophages Antibodies Goats Phenotypes Respiratory Disorders |
title_short |
Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth |
title_full |
Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth |
title_fullStr |
Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth |
title_full_unstemmed |
Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth |
title_sort |
Characterization of innate immune response to Brucella melitensis infection in goats with permissive or restrictive phenotype for Brucella intramacrophagic growth |
dc.creator.none.fl_str_mv |
Maurizio, Estefanía Trangoni, Marcos David Rossi, Ursula Dunleavy, Mariana Victoria Colato, Claudio Rossetti, Carlos Alberto |
author |
Maurizio, Estefanía |
author_facet |
Maurizio, Estefanía Trangoni, Marcos David Rossi, Ursula Dunleavy, Mariana Victoria Colato, Claudio Rossetti, Carlos Alberto |
author_role |
author |
author2 |
Trangoni, Marcos David Rossi, Ursula Dunleavy, Mariana Victoria Colato, Claudio Rossetti, Carlos Alberto |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Caprinae Brucelosis Macrofagos Anticuerpos Caprinos Brucella melitensis Fenotipos Trastornos respiratorios Brucellosis Macrophages Antibodies Goats Phenotypes Respiratory Disorders |
topic |
Caprinae Brucelosis Macrofagos Anticuerpos Caprinos Brucella melitensis Fenotipos Trastornos respiratorios Brucellosis Macrophages Antibodies Goats Phenotypes Respiratory Disorders |
dc.description.none.fl_txt_mv |
Caprine brucellosis is a chronic, world-wide distributed disease which causes reproductive failure in goats and Brucella melitensis, its causative agent, bears a great zoonotic potential. There is evidence suggesting that some cattle and pigs have an innate ability to resist Brucella infection, but this has not yet been investigated in goats. In this study, we compared caprine macrophages that exhibit extreme restriction and permissiveness to B. melitensis’ intracellular growth in vitro. Monocyte derived macrophages (MDMs) from 110 female goats were cultured and challenged in vitro with B. melitensis 16 M. After initial screening, 18 donor goats were selected based on their macrophages ability to restrict or allow bacterial intracellular growth and some elements of humoral and cellular immunity were studied in depth. MDMs that were able to restrict the pathogen’s intracellular growth showed enhanced bacterial internalization, although there were no differences between groups in the production of reactive oxygen and nitrogen intermediates following 48 h treatment with heat-killed B. melitensis. Moreover, there were no differences between groups in the level of antibodies reacting with keyhole limpet hemocyanin (natural antibodies, NAbs) or with Brucella LPS antigens (cross-reacting antibodies, CrAbs), although a strong positive correlation between individual levels of IgM NAbs and IgM CrAbs was detected. Altogether, these results represent an initial step in understanding innate primary host response to B. melitensis, and deciphering which mechanisms may determine a successful outcome of the infection in goats. Instituto de Patobiología Fil: Maurizio, Estefania. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Maurizio, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Trangoni, Marcos David. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular. Argentina Fil: Trangoni, Marcos David. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Rossi, Ursula. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Rossi, Ursula. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dunleavy, Mariana Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Dunleavy, Mariana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Colato, Claudio. Cabaña Nuevo Milenium; Argentina Fil: Rossetti, Carlos Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina Fil: Rossetti, Carlos Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Caprine brucellosis is a chronic, world-wide distributed disease which causes reproductive failure in goats and Brucella melitensis, its causative agent, bears a great zoonotic potential. There is evidence suggesting that some cattle and pigs have an innate ability to resist Brucella infection, but this has not yet been investigated in goats. In this study, we compared caprine macrophages that exhibit extreme restriction and permissiveness to B. melitensis’ intracellular growth in vitro. Monocyte derived macrophages (MDMs) from 110 female goats were cultured and challenged in vitro with B. melitensis 16 M. After initial screening, 18 donor goats were selected based on their macrophages ability to restrict or allow bacterial intracellular growth and some elements of humoral and cellular immunity were studied in depth. MDMs that were able to restrict the pathogen’s intracellular growth showed enhanced bacterial internalization, although there were no differences between groups in the production of reactive oxygen and nitrogen intermediates following 48 h treatment with heat-killed B. melitensis. Moreover, there were no differences between groups in the level of antibodies reacting with keyhole limpet hemocyanin (natural antibodies, NAbs) or with Brucella LPS antigens (cross-reacting antibodies, CrAbs), although a strong positive correlation between individual levels of IgM NAbs and IgM CrAbs was detected. Altogether, these results represent an initial step in understanding innate primary host response to B. melitensis, and deciphering which mechanisms may determine a successful outcome of the infection in goats. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-13T15:33:04Z 2021-04-13T15:33:04Z 2021-04 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/9079 https://www.sciencedirect.com/science/article/abs/pii/S0165242721000416 0165-2427 https://doi.org/10.1016/j.vetimm.2021.110223 |
url |
http://hdl.handle.net/20.500.12123/9079 https://www.sciencedirect.com/science/article/abs/pii/S0165242721000416 https://doi.org/10.1016/j.vetimm.2021.110223 |
identifier_str_mv |
0165-2427 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repograntAgreement/INTA/PNSA-1115052/AR./Epidemiología y desarrollo de estrategias para la prevención y control de enfermedades que afectan la salud pública, enfermedades exóticas y limitantes del comercio internacional. |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
Veterinary Immunology and Immunopathology 234 : 110223 (Abril 2021) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
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INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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