Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds

Autores
Fernández Vizcaíno, Elena; Poliserpi, Maria Belen; Ortiz- Santaliestra, Manuel E.; Mougeot, Francois; Mateo, Rafael; Camarero, Pablo R.; Brodeur, Celine Marie
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Thiamethoxam (TMX) is a neonicotinoid insecticide that is widely used for seed treatment in a variety of crops. Farmland birds can be exposed to TMX by consuming treated seeds remaining on the soil surface due to either spills or failed implantation during sowing. In the environment, TMX can be metabolized into clothianidin (CLO), another neonicotinoid insecticide that is also toxic to birds. The goal of the present study was to evaluate the feasibility of a non-invasive approach consisting in the analysis of fecal samples to detect recent exposure of wild birds to TMX and CLO. To achieve this, 16 grayish baywings (Agelaioides badius) were exposed for 21 days to seeds coated with four different concentrations of TMX: 0 (control), 0.027 (low), 0.33 (medium), and 4.3 (high) g TMX/kg of seed. These concentrations are within the approved range for seed coating with TMX in typical crops of the Pampa Region in Argentina, where baywings are common. Seed consumption was monitored over 24 h and fecal samples were collected on day 1, 2, 3, 4, 7, and 21 of exposure. Finally, concentrations TMX and CLO were determined in collected feces through LC-MS/MS. Fecal concentrations of both TMX and CLO showed a significant dependence on the treatment dose (p = 0.006). Average concentrations of TMX and CLO were respectively 63, 689, and 2363 ng/g and 75, 735 and 2040 ng/g for low, medium and high dose groups. These results demonstrate that fecal analysis is a reliable non-invasive method for assessing recent bird exposure to TMX. The results also confirm that TMX is metabolized into CLO, and suggest a saturation of the metabolic pathway at high doses. This study supports the use of fecal analysis for pesticide exposure estimation in field monitoring studies of wild birds.
Instituto de Recursos Biológicos
Fil: Fernández-Vizcaíno, Elena. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España
Fil: Poliserpi, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Ortiz-Santaliestra, Manuel E. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España
Fil: Mougeot, Francois. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España
Fil: Mateo, Rafael. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España. CSIC. Institute for Environmental Assessment and Water Research (IDAEA); España
Fil: Camarero, Pablo R. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España
Fil: Brodeur, Julie C. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Brodeur, Julie C. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Environmental Pollution 382 : 126707. (October 2025)
Materia
Faeces
Pesticide Exposure
Birds
Neonicotinoid Insecticides
Thiamethoxam
Heces
Exposición al Pesticida
Pájaros
Insecticida Neonicotinoide
Tiametoxam
Monitoreo no Invasivo
Semillas Tratadas
Non-invasive Monitoring
Treated Seeds
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22884
Acceder
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oai_identifier_str oai:localhost:20.500.12123/22884
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birdsFernández Vizcaíno, ElenaPoliserpi, Maria BelenOrtiz- Santaliestra, Manuel E.Mougeot, FrancoisMateo, RafaelCamarero, Pablo R.Brodeur, Celine MarieFaecesPesticide ExposureBirdsNeonicotinoid InsecticidesThiamethoxamHecesExposición al PesticidaPájarosInsecticida NeonicotinoideTiametoxamMonitoreo no InvasivoSemillas TratadasNon-invasive MonitoringTreated SeedsThiamethoxam (TMX) is a neonicotinoid insecticide that is widely used for seed treatment in a variety of crops. Farmland birds can be exposed to TMX by consuming treated seeds remaining on the soil surface due to either spills or failed implantation during sowing. In the environment, TMX can be metabolized into clothianidin (CLO), another neonicotinoid insecticide that is also toxic to birds. The goal of the present study was to evaluate the feasibility of a non-invasive approach consisting in the analysis of fecal samples to detect recent exposure of wild birds to TMX and CLO. To achieve this, 16 grayish baywings (Agelaioides badius) were exposed for 21 days to seeds coated with four different concentrations of TMX: 0 (control), 0.027 (low), 0.33 (medium), and 4.3 (high) g TMX/kg of seed. These concentrations are within the approved range for seed coating with TMX in typical crops of the Pampa Region in Argentina, where baywings are common. Seed consumption was monitored over 24 h and fecal samples were collected on day 1, 2, 3, 4, 7, and 21 of exposure. Finally, concentrations TMX and CLO were determined in collected feces through LC-MS/MS. Fecal concentrations of both TMX and CLO showed a significant dependence on the treatment dose (p = 0.006). Average concentrations of TMX and CLO were respectively 63, 689, and 2363 ng/g and 75, 735 and 2040 ng/g for low, medium and high dose groups. These results demonstrate that fecal analysis is a reliable non-invasive method for assessing recent bird exposure to TMX. The results also confirm that TMX is metabolized into CLO, and suggest a saturation of the metabolic pathway at high doses. This study supports the use of fecal analysis for pesticide exposure estimation in field monitoring studies of wild birds.Instituto de Recursos BiológicosFil: Fernández-Vizcaíno, Elena. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); EspañaFil: Poliserpi, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Ortiz-Santaliestra, Manuel E. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); EspañaFil: Mougeot, Francois. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); EspañaFil: Mateo, Rafael. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España. CSIC. Institute for Environmental Assessment and Water Research (IDAEA); EspañaFil: Camarero, Pablo R. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); EspañaFil: Brodeur, Julie C. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; ArgentinaFil: Brodeur, Julie C. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2025-07-03T13:42:15Z2025-07-03T13:42:15Z2025-06-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22884https://www.sciencedirect.com/science/article/pii/S02697491250108020269-74911873-6424https://doi.org/10.1016/j.envpol.2025.126707Environmental Pollution 382 : 126707. (October 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L03-I094, Gestión ambiental de los agroquímicos: manejo, mitigación y remediacióninfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:51:09Zoai:localhost:20.500.12123/22884instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:51:10.242INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds
title Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds
spellingShingle Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds
Fernández Vizcaíno, Elena
Faeces
Pesticide Exposure
Birds
Neonicotinoid Insecticides
Thiamethoxam
Heces
Exposición al Pesticida
Pájaros
Insecticida Neonicotinoide
Tiametoxam
Monitoreo no Invasivo
Semillas Tratadas
Non-invasive Monitoring
Treated Seeds
title_short Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds
title_full Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds
title_fullStr Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds
title_full_unstemmed Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds
title_sort Monitoring approach to detect recent exposure to thiamethoxam-treated seeds in birds
dc.creator.none.fl_str_mv Fernández Vizcaíno, Elena
Poliserpi, Maria Belen
Ortiz- Santaliestra, Manuel E.
Mougeot, Francois
Mateo, Rafael
Camarero, Pablo R.
Brodeur, Celine Marie
author Fernández Vizcaíno, Elena
author_facet Fernández Vizcaíno, Elena
Poliserpi, Maria Belen
Ortiz- Santaliestra, Manuel E.
Mougeot, Francois
Mateo, Rafael
Camarero, Pablo R.
Brodeur, Celine Marie
author_role author
author2 Poliserpi, Maria Belen
Ortiz- Santaliestra, Manuel E.
Mougeot, Francois
Mateo, Rafael
Camarero, Pablo R.
Brodeur, Celine Marie
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Faeces
Pesticide Exposure
Birds
Neonicotinoid Insecticides
Thiamethoxam
Heces
Exposición al Pesticida
Pájaros
Insecticida Neonicotinoide
Tiametoxam
Monitoreo no Invasivo
Semillas Tratadas
Non-invasive Monitoring
Treated Seeds
topic Faeces
Pesticide Exposure
Birds
Neonicotinoid Insecticides
Thiamethoxam
Heces
Exposición al Pesticida
Pájaros
Insecticida Neonicotinoide
Tiametoxam
Monitoreo no Invasivo
Semillas Tratadas
Non-invasive Monitoring
Treated Seeds
dc.description.none.fl_txt_mv Thiamethoxam (TMX) is a neonicotinoid insecticide that is widely used for seed treatment in a variety of crops. Farmland birds can be exposed to TMX by consuming treated seeds remaining on the soil surface due to either spills or failed implantation during sowing. In the environment, TMX can be metabolized into clothianidin (CLO), another neonicotinoid insecticide that is also toxic to birds. The goal of the present study was to evaluate the feasibility of a non-invasive approach consisting in the analysis of fecal samples to detect recent exposure of wild birds to TMX and CLO. To achieve this, 16 grayish baywings (Agelaioides badius) were exposed for 21 days to seeds coated with four different concentrations of TMX: 0 (control), 0.027 (low), 0.33 (medium), and 4.3 (high) g TMX/kg of seed. These concentrations are within the approved range for seed coating with TMX in typical crops of the Pampa Region in Argentina, where baywings are common. Seed consumption was monitored over 24 h and fecal samples were collected on day 1, 2, 3, 4, 7, and 21 of exposure. Finally, concentrations TMX and CLO were determined in collected feces through LC-MS/MS. Fecal concentrations of both TMX and CLO showed a significant dependence on the treatment dose (p = 0.006). Average concentrations of TMX and CLO were respectively 63, 689, and 2363 ng/g and 75, 735 and 2040 ng/g for low, medium and high dose groups. These results demonstrate that fecal analysis is a reliable non-invasive method for assessing recent bird exposure to TMX. The results also confirm that TMX is metabolized into CLO, and suggest a saturation of the metabolic pathway at high doses. This study supports the use of fecal analysis for pesticide exposure estimation in field monitoring studies of wild birds.
Instituto de Recursos Biológicos
Fil: Fernández-Vizcaíno, Elena. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España
Fil: Poliserpi, María Belén. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Ortiz-Santaliestra, Manuel E. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España
Fil: Mougeot, Francois. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España
Fil: Mateo, Rafael. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España. CSIC. Institute for Environmental Assessment and Water Research (IDAEA); España
Fil: Camarero, Pablo R. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC); España
Fil: Brodeur, Julie C. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Recursos Biológicos; Argentina
Fil: Brodeur, Julie C. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Thiamethoxam (TMX) is a neonicotinoid insecticide that is widely used for seed treatment in a variety of crops. Farmland birds can be exposed to TMX by consuming treated seeds remaining on the soil surface due to either spills or failed implantation during sowing. In the environment, TMX can be metabolized into clothianidin (CLO), another neonicotinoid insecticide that is also toxic to birds. The goal of the present study was to evaluate the feasibility of a non-invasive approach consisting in the analysis of fecal samples to detect recent exposure of wild birds to TMX and CLO. To achieve this, 16 grayish baywings (Agelaioides badius) were exposed for 21 days to seeds coated with four different concentrations of TMX: 0 (control), 0.027 (low), 0.33 (medium), and 4.3 (high) g TMX/kg of seed. These concentrations are within the approved range for seed coating with TMX in typical crops of the Pampa Region in Argentina, where baywings are common. Seed consumption was monitored over 24 h and fecal samples were collected on day 1, 2, 3, 4, 7, and 21 of exposure. Finally, concentrations TMX and CLO were determined in collected feces through LC-MS/MS. Fecal concentrations of both TMX and CLO showed a significant dependence on the treatment dose (p = 0.006). Average concentrations of TMX and CLO were respectively 63, 689, and 2363 ng/g and 75, 735 and 2040 ng/g for low, medium and high dose groups. These results demonstrate that fecal analysis is a reliable non-invasive method for assessing recent bird exposure to TMX. The results also confirm that TMX is metabolized into CLO, and suggest a saturation of the metabolic pathway at high doses. This study supports the use of fecal analysis for pesticide exposure estimation in field monitoring studies of wild birds.
publishDate 2025
dc.date.none.fl_str_mv 2025-07-03T13:42:15Z
2025-07-03T13:42:15Z
2025-06-19
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22884
https://www.sciencedirect.com/science/article/pii/S0269749125010802
0269-7491
1873-6424
https://doi.org/10.1016/j.envpol.2025.126707
url http://hdl.handle.net/20.500.12123/22884
https://www.sciencedirect.com/science/article/pii/S0269749125010802
https://doi.org/10.1016/j.envpol.2025.126707
identifier_str_mv 0269-7491
1873-6424
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L03-I094, Gestión ambiental de los agroquímicos: manejo, mitigación y remediación
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Environmental Pollution 382 : 126707. (October 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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