Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina

Autores
Samarfard, Samira; Bejerman, Nicolas Esteban; Dietzgen, Ralf Georg
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In 2010, a novel cytorhabdovirus named alfalfa dwarf virus (ADV) was detected for the first time in lucerne crops in Argentina showing dwarfism, in mixed infections with several other viruses. ADV appears to be endemic to Argentina and has not been reported elsewhere. In this study, we have investigated the genetic variability of ADV based on the complete nucleoprotein (N) gene of 13 isolates from different lucerne-growing regions in Argentina. Phylogenetic and sequence identity analyses showed that all ADV isolates are closely related and have not diverged more than 1% in the N gene despite geographical separation. These data provide further evidence that ADV is new to science and emerged and spread very recently. A total of 43 single-nucleotide polymorphisms were identified between the ADV isolates studied. Analysis of N gene ORF sequence revealed a mutational bias, with more transitions than transversions. In all cases, the ratio of non-synonymous/synonymous nucleotide changes was < 1, indicating that ADV N gene is under predominantly purifying selection.
Instituto de Patología Vegetal
Fil: Samarfard, Samira. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dietzgen, Ralf Georg. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fuente
Virus genes 54 (4) : 612–615. (August 2018)
Materia
Medicago Sativa
Virus de las Plantas
Plant Viruses
Genetic Variation
Variación Genética
Cytorhabdovirus
Polymorphism
Polimorfismo
Nucleoproteins
Nucleoproteínas
Alfalfa
Alfalfa Dwarf Virus
Phylogenetic Analysis
Análisis Filogenético
Single-nucleotide Polymorphism
Nucleoprotein Gene
Virus del Enanismo de la Alfalfa
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3630
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3630
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in ArgentinaSamarfard, SamiraBejerman, Nicolas EstebanDietzgen, Ralf GeorgMedicago SativaVirus de las PlantasPlant VirusesGenetic VariationVariación GenéticaCytorhabdovirusPolymorphismPolimorfismoNucleoproteinsNucleoproteínasAlfalfaAlfalfa Dwarf VirusPhylogenetic AnalysisAnálisis FilogenéticoSingle-nucleotide PolymorphismNucleoprotein GeneVirus del Enanismo de la AlfalfaIn 2010, a novel cytorhabdovirus named alfalfa dwarf virus (ADV) was detected for the first time in lucerne crops in Argentina showing dwarfism, in mixed infections with several other viruses. ADV appears to be endemic to Argentina and has not been reported elsewhere. In this study, we have investigated the genetic variability of ADV based on the complete nucleoprotein (N) gene of 13 isolates from different lucerne-growing regions in Argentina. Phylogenetic and sequence identity analyses showed that all ADV isolates are closely related and have not diverged more than 1% in the N gene despite geographical separation. These data provide further evidence that ADV is new to science and emerged and spread very recently. A total of 43 single-nucleotide polymorphisms were identified between the ADV isolates studied. Analysis of N gene ORF sequence revealed a mutational bias, with more transitions than transversions. In all cases, the ratio of non-synonymous/synonymous nucleotide changes was < 1, indicating that ADV N gene is under predominantly purifying selection.Instituto de Patología VegetalFil: Samarfard, Samira. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; AustraliaFil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dietzgen, Ralf Georg. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; AustraliaSpringer2018-10-18T14:27:29Z2018-10-18T14:27:29Z2018-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3630https://link.springer.com/article/10.1007%2Fs11262-018-1563-2#citeas0920-85691572-994X (Online)https://doi.org/10.1007/s11262-018-1563-2Virus genes 54 (4) : 612–615. (August 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina (nation)info:eu-repo/semantics/restrictedAccess2025-10-23T11:16:42Zoai:localhost:20.500.12123/3630instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:16:42.368INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina
title Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina
spellingShingle Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina
Samarfard, Samira
Medicago Sativa
Virus de las Plantas
Plant Viruses
Genetic Variation
Variación Genética
Cytorhabdovirus
Polymorphism
Polimorfismo
Nucleoproteins
Nucleoproteínas
Alfalfa
Alfalfa Dwarf Virus
Phylogenetic Analysis
Análisis Filogenético
Single-nucleotide Polymorphism
Nucleoprotein Gene
Virus del Enanismo de la Alfalfa
title_short Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina
title_full Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina
title_fullStr Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina
title_full_unstemmed Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina
title_sort Distribution and genetic variability of alfalfa dwarf virus, a cytorhabdovirus associated with alfalfa dwarf disease in Argentina
dc.creator.none.fl_str_mv Samarfard, Samira
Bejerman, Nicolas Esteban
Dietzgen, Ralf Georg
author Samarfard, Samira
author_facet Samarfard, Samira
Bejerman, Nicolas Esteban
Dietzgen, Ralf Georg
author_role author
author2 Bejerman, Nicolas Esteban
Dietzgen, Ralf Georg
author2_role author
author
dc.subject.none.fl_str_mv Medicago Sativa
Virus de las Plantas
Plant Viruses
Genetic Variation
Variación Genética
Cytorhabdovirus
Polymorphism
Polimorfismo
Nucleoproteins
Nucleoproteínas
Alfalfa
Alfalfa Dwarf Virus
Phylogenetic Analysis
Análisis Filogenético
Single-nucleotide Polymorphism
Nucleoprotein Gene
Virus del Enanismo de la Alfalfa
topic Medicago Sativa
Virus de las Plantas
Plant Viruses
Genetic Variation
Variación Genética
Cytorhabdovirus
Polymorphism
Polimorfismo
Nucleoproteins
Nucleoproteínas
Alfalfa
Alfalfa Dwarf Virus
Phylogenetic Analysis
Análisis Filogenético
Single-nucleotide Polymorphism
Nucleoprotein Gene
Virus del Enanismo de la Alfalfa
dc.description.none.fl_txt_mv In 2010, a novel cytorhabdovirus named alfalfa dwarf virus (ADV) was detected for the first time in lucerne crops in Argentina showing dwarfism, in mixed infections with several other viruses. ADV appears to be endemic to Argentina and has not been reported elsewhere. In this study, we have investigated the genetic variability of ADV based on the complete nucleoprotein (N) gene of 13 isolates from different lucerne-growing regions in Argentina. Phylogenetic and sequence identity analyses showed that all ADV isolates are closely related and have not diverged more than 1% in the N gene despite geographical separation. These data provide further evidence that ADV is new to science and emerged and spread very recently. A total of 43 single-nucleotide polymorphisms were identified between the ADV isolates studied. Analysis of N gene ORF sequence revealed a mutational bias, with more transitions than transversions. In all cases, the ratio of non-synonymous/synonymous nucleotide changes was < 1, indicating that ADV N gene is under predominantly purifying selection.
Instituto de Patología Vegetal
Fil: Samarfard, Samira. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
Fil: Bejerman, Nicolas Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dietzgen, Ralf Georg. University of Queensland. Queensland Alliance for Agriculture and Food Innovation; Australia
description In 2010, a novel cytorhabdovirus named alfalfa dwarf virus (ADV) was detected for the first time in lucerne crops in Argentina showing dwarfism, in mixed infections with several other viruses. ADV appears to be endemic to Argentina and has not been reported elsewhere. In this study, we have investigated the genetic variability of ADV based on the complete nucleoprotein (N) gene of 13 isolates from different lucerne-growing regions in Argentina. Phylogenetic and sequence identity analyses showed that all ADV isolates are closely related and have not diverged more than 1% in the N gene despite geographical separation. These data provide further evidence that ADV is new to science and emerged and spread very recently. A total of 43 single-nucleotide polymorphisms were identified between the ADV isolates studied. Analysis of N gene ORF sequence revealed a mutational bias, with more transitions than transversions. In all cases, the ratio of non-synonymous/synonymous nucleotide changes was < 1, indicating that ADV N gene is under predominantly purifying selection.
publishDate 2018
dc.date.none.fl_str_mv 2018-10-18T14:27:29Z
2018-10-18T14:27:29Z
2018-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3630
https://link.springer.com/article/10.1007%2Fs11262-018-1563-2#citeas
0920-8569
1572-994X (Online)
https://doi.org/10.1007/s11262-018-1563-2
url http://hdl.handle.net/20.500.12123/3630
https://link.springer.com/article/10.1007%2Fs11262-018-1563-2#citeas
https://doi.org/10.1007/s11262-018-1563-2
identifier_str_mv 0920-8569
1572-994X (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina (nation)
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Virus genes 54 (4) : 612–615. (August 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1846787513498206208
score 12.982451

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