A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile

Autores
Ogrzewalska, María; Nieri-Bastos, Fernanda A.; Marcili, Arlei; Nava, Santiago; González-Acuña, Daniel; Muñoz-Leal, Sebastian; Ruiz-Arrondo, Ignacio; Venzal, José Manuel; Mangold, Atilio Jose; Labruna, Marcelo B.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
The tick Amblyomma parvitarsum (Acari: Ixodidae) has established populations in Andean and Patagonic environments of South America. For the present study, adults of A. parvitarsum were collected in highland areas (elevation >3500 m) of Argentina and Chile during 2009–2013, and tested by PCR for rickettsial infection in the laboratory, and isolation of rickettsiae in Vero cell culture by the shell vial technique. Overall, 51 (62.2%) out of 82 A. parvitarsum adult ticks were infected by spotted fever group (SFG) rickettsiae, which generated DNA sequences 100% identical to each other, and when submitted to BLAST analysis, they were 99.3% identical to corresponding sequence of the ompA gene of Rickettsia sp. strain Atlantic rainforest. Rickettsiae were successfully isolated in Vero cell culture from two ticks, one from Argentina and one from Chile. DNA extracted from the third passage of the isolates of Argentina and Chile were processed by PCR, resulting in partial sequences for three rickettsial genes (gltA, ompB, ompA). These sequences were concatenated and aligned with rickettsial corresponding sequences available in GenBank. Phylogenetic analysis revealed that the A. pavitarsum rickettsial agent grouped under high bootstrap support in a clade composed by the SFG pathogens R. sibirica, R. africae, R. parkeri, Rickettsia sp. strain Atlantic rainforest, and two unnamed SFG agents of unknown pathogenicty, Rickettsia sp. strain NOD, and Rickettsia sp. strain ApPR. The pathogenic role of this A. parvitarsum rickettsia cannot be discarded, since several species of tick-borne rickettsiae that were considered nonpathogenic for decades are now associated with human infections.
EEA Rafaela
Fil: Ogrzewalska, María. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses; Brasil
Fil: Nieri-Bastos, Fernanda A. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
Fil: Marcili, Arlei. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil. Universidade de Santo Amaro. Medicina Veterinária e Bem estar animal; Brasil
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: González-Acuña, Daniel. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chile
Fil: Muñoz-Leal, Sebastian. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chile
Fil: Ruiz-Arrondo, Ignacio. Universidad de Zaragoza. Departamento de Patología Animal, Facultad de Veterinaria; España
Fil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte. Laboratorio de Vectores y enfermedades transmitidas; Uruguay
Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Labruna, Marcelo B. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
Fuente
Ticks and tick-borne diseases 7 (3) : 439-442. (April 2016)
Materia
Rickettsia
Amblyomma
Zona de Montaña
Highlands
Región Patagónica
Amblyomma Parvitarsum
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1134
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1134
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network_name_str INTA Digital (INTA)
spelling A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and ChileOgrzewalska, MaríaNieri-Bastos, Fernanda A.Marcili, ArleiNava, SantiagoGonzález-Acuña, DanielMuñoz-Leal, SebastianRuiz-Arrondo, IgnacioVenzal, José ManuelMangold, Atilio JoseLabruna, Marcelo B.RickettsiaAmblyommaZona de MontañaHighlandsRegión PatagónicaAmblyomma ParvitarsumThe tick Amblyomma parvitarsum (Acari: Ixodidae) has established populations in Andean and Patagonic environments of South America. For the present study, adults of A. parvitarsum were collected in highland areas (elevation >3500 m) of Argentina and Chile during 2009–2013, and tested by PCR for rickettsial infection in the laboratory, and isolation of rickettsiae in Vero cell culture by the shell vial technique. Overall, 51 (62.2%) out of 82 A. parvitarsum adult ticks were infected by spotted fever group (SFG) rickettsiae, which generated DNA sequences 100% identical to each other, and when submitted to BLAST analysis, they were 99.3% identical to corresponding sequence of the ompA gene of Rickettsia sp. strain Atlantic rainforest. Rickettsiae were successfully isolated in Vero cell culture from two ticks, one from Argentina and one from Chile. DNA extracted from the third passage of the isolates of Argentina and Chile were processed by PCR, resulting in partial sequences for three rickettsial genes (gltA, ompB, ompA). These sequences were concatenated and aligned with rickettsial corresponding sequences available in GenBank. Phylogenetic analysis revealed that the A. pavitarsum rickettsial agent grouped under high bootstrap support in a clade composed by the SFG pathogens R. sibirica, R. africae, R. parkeri, Rickettsia sp. strain Atlantic rainforest, and two unnamed SFG agents of unknown pathogenicty, Rickettsia sp. strain NOD, and Rickettsia sp. strain ApPR. The pathogenic role of this A. parvitarsum rickettsia cannot be discarded, since several species of tick-borne rickettsiae that were considered nonpathogenic for decades are now associated with human infections.EEA RafaelaFil: Ogrzewalska, María. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses; BrasilFil: Nieri-Bastos, Fernanda A. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; BrasilFil: Marcili, Arlei. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil. Universidade de Santo Amaro. Medicina Veterinária e Bem estar animal; BrasilFil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: González-Acuña, Daniel. Universidad de Concepción. Facultad de Ciencias Veterinarias; ChileFil: Muñoz-Leal, Sebastian. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil. Universidad de Concepción. Facultad de Ciencias Veterinarias; ChileFil: Ruiz-Arrondo, Ignacio. Universidad de Zaragoza. Departamento de Patología Animal, Facultad de Veterinaria; EspañaFil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte. Laboratorio de Vectores y enfermedades transmitidas; UruguayFil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Labruna, Marcelo B. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil2017-09-05T15:32:05Z2017-09-05T15:32:05Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1134http://www.sciencedirect.com/science/article/pii/S1877959X16300036?via%3Dihub1877-959X (Print)1877-9603 (Online)https://doi.org/10.1016/j.ttbdis.2016.01.003Ticks and tick-borne diseases 7 (3) : 439-442. (April 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina (nation)Chile (nation)info:eu-repo/semantics/restrictedAccess2025-09-29T13:44:09Zoai:localhost:20.500.12123/1134instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:10.217INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
title A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
spellingShingle A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
Ogrzewalska, María
Rickettsia
Amblyomma
Zona de Montaña
Highlands
Región Patagónica
Amblyomma Parvitarsum
title_short A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
title_full A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
title_fullStr A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
title_full_unstemmed A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
title_sort A novel spotted fever group Rickettsia infecting Amblyomma parvitarsum (Acari: Ixodidae) in highlands of Argentina and Chile
dc.creator.none.fl_str_mv Ogrzewalska, María
Nieri-Bastos, Fernanda A.
Marcili, Arlei
Nava, Santiago
González-Acuña, Daniel
Muñoz-Leal, Sebastian
Ruiz-Arrondo, Ignacio
Venzal, José Manuel
Mangold, Atilio Jose
Labruna, Marcelo B.
author Ogrzewalska, María
author_facet Ogrzewalska, María
Nieri-Bastos, Fernanda A.
Marcili, Arlei
Nava, Santiago
González-Acuña, Daniel
Muñoz-Leal, Sebastian
Ruiz-Arrondo, Ignacio
Venzal, José Manuel
Mangold, Atilio Jose
Labruna, Marcelo B.
author_role author
author2 Nieri-Bastos, Fernanda A.
Marcili, Arlei
Nava, Santiago
González-Acuña, Daniel
Muñoz-Leal, Sebastian
Ruiz-Arrondo, Ignacio
Venzal, José Manuel
Mangold, Atilio Jose
Labruna, Marcelo B.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Rickettsia
Amblyomma
Zona de Montaña
Highlands
Región Patagónica
Amblyomma Parvitarsum
topic Rickettsia
Amblyomma
Zona de Montaña
Highlands
Región Patagónica
Amblyomma Parvitarsum
dc.description.none.fl_txt_mv The tick Amblyomma parvitarsum (Acari: Ixodidae) has established populations in Andean and Patagonic environments of South America. For the present study, adults of A. parvitarsum were collected in highland areas (elevation >3500 m) of Argentina and Chile during 2009–2013, and tested by PCR for rickettsial infection in the laboratory, and isolation of rickettsiae in Vero cell culture by the shell vial technique. Overall, 51 (62.2%) out of 82 A. parvitarsum adult ticks were infected by spotted fever group (SFG) rickettsiae, which generated DNA sequences 100% identical to each other, and when submitted to BLAST analysis, they were 99.3% identical to corresponding sequence of the ompA gene of Rickettsia sp. strain Atlantic rainforest. Rickettsiae were successfully isolated in Vero cell culture from two ticks, one from Argentina and one from Chile. DNA extracted from the third passage of the isolates of Argentina and Chile were processed by PCR, resulting in partial sequences for three rickettsial genes (gltA, ompB, ompA). These sequences were concatenated and aligned with rickettsial corresponding sequences available in GenBank. Phylogenetic analysis revealed that the A. pavitarsum rickettsial agent grouped under high bootstrap support in a clade composed by the SFG pathogens R. sibirica, R. africae, R. parkeri, Rickettsia sp. strain Atlantic rainforest, and two unnamed SFG agents of unknown pathogenicty, Rickettsia sp. strain NOD, and Rickettsia sp. strain ApPR. The pathogenic role of this A. parvitarsum rickettsia cannot be discarded, since several species of tick-borne rickettsiae that were considered nonpathogenic for decades are now associated with human infections.
EEA Rafaela
Fil: Ogrzewalska, María. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses; Brasil
Fil: Nieri-Bastos, Fernanda A. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
Fil: Marcili, Arlei. Universidade Federal do ABC. Centro de Ciências Naturais e Humanas; Brasil. Universidade de Santo Amaro. Medicina Veterinária e Bem estar animal; Brasil
Fil: Nava, Santiago. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: González-Acuña, Daniel. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chile
Fil: Muñoz-Leal, Sebastian. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil. Universidad de Concepción. Facultad de Ciencias Veterinarias; Chile
Fil: Ruiz-Arrondo, Ignacio. Universidad de Zaragoza. Departamento de Patología Animal, Facultad de Veterinaria; España
Fil: Venzal, José Manuel. Universidad de la República. CENUR Litoral Norte. Laboratorio de Vectores y enfermedades transmitidas; Uruguay
Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Labruna, Marcelo B. Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Saúde Animal; Brasil
description The tick Amblyomma parvitarsum (Acari: Ixodidae) has established populations in Andean and Patagonic environments of South America. For the present study, adults of A. parvitarsum were collected in highland areas (elevation >3500 m) of Argentina and Chile during 2009–2013, and tested by PCR for rickettsial infection in the laboratory, and isolation of rickettsiae in Vero cell culture by the shell vial technique. Overall, 51 (62.2%) out of 82 A. parvitarsum adult ticks were infected by spotted fever group (SFG) rickettsiae, which generated DNA sequences 100% identical to each other, and when submitted to BLAST analysis, they were 99.3% identical to corresponding sequence of the ompA gene of Rickettsia sp. strain Atlantic rainforest. Rickettsiae were successfully isolated in Vero cell culture from two ticks, one from Argentina and one from Chile. DNA extracted from the third passage of the isolates of Argentina and Chile were processed by PCR, resulting in partial sequences for three rickettsial genes (gltA, ompB, ompA). These sequences were concatenated and aligned with rickettsial corresponding sequences available in GenBank. Phylogenetic analysis revealed that the A. pavitarsum rickettsial agent grouped under high bootstrap support in a clade composed by the SFG pathogens R. sibirica, R. africae, R. parkeri, Rickettsia sp. strain Atlantic rainforest, and two unnamed SFG agents of unknown pathogenicty, Rickettsia sp. strain NOD, and Rickettsia sp. strain ApPR. The pathogenic role of this A. parvitarsum rickettsia cannot be discarded, since several species of tick-borne rickettsiae that were considered nonpathogenic for decades are now associated with human infections.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-09-05T15:32:05Z
2017-09-05T15:32:05Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1134
http://www.sciencedirect.com/science/article/pii/S1877959X16300036?via%3Dihub
1877-959X (Print)
1877-9603 (Online)
https://doi.org/10.1016/j.ttbdis.2016.01.003
url http://hdl.handle.net/20.500.12123/1134
http://www.sciencedirect.com/science/article/pii/S1877959X16300036?via%3Dihub
https://doi.org/10.1016/j.ttbdis.2016.01.003
identifier_str_mv 1877-959X (Print)
1877-9603 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina (nation)
Chile (nation)
dc.source.none.fl_str_mv Ticks and tick-borne diseases 7 (3) : 439-442. (April 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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