Nitrogen but not phosphorus addition affects symbiotic N2 fixation by legumes in natural and semi‑natural grasslands located on four continents

• Autores: Vázquez, Eduardo; Schleuss, Per‑Marten; Borer, Elizabeth T.; Bugalho, Miguel N.; Caldeira, Maria C.; Eisenhauer, Nico; Eskelinen, Anu; Fay, Philip A.; Haider, Sylvia; Jentsch, Anke; Kirkman, Kevin P.; McCulley, Rebecca L.; Peri, Pablo Luis; Price, Jodi; Richards, Anna E.; Risch, Anita C.; Roscher, Christiane; Schütz, Martin; Seabloom, Eric William; Standish, Rachel J.; Stevens, Carly J.; Tedder, Michelle J.; Virtanen, Risto; Spohn, Marie
• Año de publicación: 2022
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Plant and Soil 478 : 689-707. (2022)
• Materia: Grasslands; Legumes; Nitrogen; Fertilizer Application; Phosphorus; Isotope Analysis; Symbiosis; Pastizales; Leguminosas; Nitrógeno; Aplicación de Abonos; Fósforo; Simbiosis; Nutrient Network (NutNet); Fixation; Red de Nutrientes; Fijación; Fertilización
• Institución: Instituto Nacional de Tecnología Agropecuaria

The amount of nitrogen (N) derived from symbiotic N2 fixation by legumes in grasslands might be affected by anthropogenic N and phosphorus (P) inputs, but the underlying mechanisms are not known. Methods We evaluated symbiotic N2 fixation in 17 natural and semi-natural grasslands on four continents that are subjected to the same full-factorial N and P addition experiment, using the 15N natural abundance method. Results N as well as combined N and P (NP) addition reduced aboveground legume biomass by 65% and 45%, respectively, compared to the control, whereas P addition had no significant impact. Addition of N and/or P had no significant effect on the symbiotic N2 fixation per unit legume biomass. In consequence, the amount of N fixed annually per grassland area was less than half in the N addition treatments compared to control and P addition, irrespective of whether the dominant legumes were annuals or perennials. Conclusion Our results reveal that N addition mainly impacts symbiotic N2 fixation via reduced biomass of legumes rather than changes in N2 fixation per unit legume biomass. The results show that soil N enrichment by anthropogenic activities significantly reduces N 2 fixation in grasslands, and these effects cannot be reversed by additional P amendment.
EEA Santa Cruz
Fil: Vázquez, Eduardo. University of Bayreuth. Department of Soil Ecology. Bayreuth Center of Ecology and Environmental Research (BayCEER); Alemania
Fil: Vázquez, Eduardo. Swedish University of Agricultural Sciences. Department of Soil and Environment; Suecia
Fil: Schleuss, Per‑Marten. University of Bayreuth. Department of Soil Ecology. Bayreuth Center of Ecology and Environmental Research (BayCEER); Alemania
Fil: Borer, Elizabeth T. University of Minnesota. Department of Ecology, Evolution, and Behavior; Estados Unidos
Fil: Bugalho, Miguel N. University of Lisbon. Centre for Applied Ecology “Prof. Baeta Neves” (CEABN-InBIO). School of Agriculture; Portugal.
Fil: Caldeira, Maria. C. University of Lisbon. Forest Research Centre. School of Agriculture; Portugal.
Fil: Eisenhauer, Nico. German Centre for Integrative Biodiversity Research; Alemania
Fil: Eisenhauer, Nico. Leipzig University. Institute of Biology; Alemania
Fil: Eskelinen, Anu. German Centre for Integrative Biodiversity Research; Alemania
Fil: Eskelinen, Anu. Physiological Diversity, Helmholtz Centrefor Environmental Research; Alemania
Fil: Eskelinen, Anu. University of Oulu. Ecology & Genetics; Finlandia
Fil: Fay, Philip A. Grassland Soil and Water Research Laboratory (USDA-ARS); Estados Unidos
Fil: Haider, Sylvia. German Centre for Integrative Biodiversity Research; Alemania
Fil: Haider, Sylvia. Martin Luther University. Institute of Biology. Geobotany and Botanical Garden; Alemania
Fil: Jentsch, Anke. University of Bayreuth. Department of Soil Ecology. Bayreuth Center of Ecology and Environmental Research (BayCEER); Alemania
Fil: Kirkman, Kevin P. University of KwaZulu-Natal. School of Life Sciences; Sudáfrica
Fil: McCulley, Rebecca L. University of Kentucky. Department of Plant and Soil Sciences; Estados Unidos
Fil: Peri, Pablo Luis. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santa Cruz; Argentina.
Fil: Peri, Pablo Luis. Universidad Nacional de la Patagonia Austral; Argentina.
Fil: Peri, Pablo Luis. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Price, Jodi. Charles Sturt University. Institute for Land, Water and Society; Australia.
Fil: Richards, Anna E. CSIRO Land and Water. Northern Territory; Australia.
Fil: Risch, Anita C. Swiss Federal Institute for Forest, Snow and Landscape Research WSL; Suiza
Fil: Roscher, Christiane. German Centre for Integrative Biodiversity Research; Alemania
Fil: Roscher, Christiane. Physiological Diversity, Helmholtz Centre for Environmental Research; Alemania
Fil: Schütz, Martin. Swiss Federal Institute for Forest, Snow and Landscape Research WSL; Suiza
Fil: Seabloom, Eric William. University of Minnesota. Dept. of Ecology, Evolution, and Behavior; Estados Unidos
Fil: Standish, Rachel J. Murdoch University. Harry Butler Institute; Australia.
Fil: Stevens, Carly J. Lancaster University. Lancaster Environment Centre; Reino Unido
Fil: Tedder, Michelle J. University of KwaZulu-Natal. School of Life Sciences; Sudáfrica
Fil: Virtanen, Risto. University of Oulu. Ecology & Genetics; Finlandia.
Fil: Spohn, Marie. University of Bayreuth. Department of Soil Ecology. Bayreuth Center of Ecology and Environmental Research (BayCEER); Alemania
Fil: Spohn, Marie. Swedish University of Agricultural Sciences. Department of Soil and Environment; Suecia