Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH)

Autores
Giardini, Maria Cecilia; Milla, Fabian Horacio; Conte, Claudia Alejandra; Lanzavecchia, Silvia Beatriz; Nieves, Mariela
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: The South American fruit fly Anastrepha fraterculus sp. 1 belongs to a cryptic species complex with only a single morphotype present in Argentina, which possess polymorphisms in the sex chromosomes, identified by two X (X1, X2) and two Y (Y5, Y6) variants. Our aim was to explore the molecular composition of the sex chromosomes identified in the laboratory strain Af-Y-short due to it possesses the most frequent sex chromosomes variants, and the smallest Y chromosome found in wild populations and laboratory colonies of A. fraterculus sp. 1. Methods and results: Whole Comparative Genomic Hybridization (W-CGH) was applied to the Af-Y-short laboratory strain to identify potential differences in the size and composition of highly repetitive DNA blocks between individuals, morphs or sexes. Our results showed that the X1 and X2 share complete homology in euchromatic regions, also sharing similar sequences with the Y5 chromosome in the centromeric region. Females X1X1 and X1X2 exhibited repetitive DNA in telomeric regions which contributed to their morphological differentiation, while the Y chromosome evidenced the accumulation of specific and differential repetitive sequences. In contrast, autosomes showed balanced signal with no sex-chromosome-specific sequence accumulation. Conclusions: Our findings highlight the role of repetitive DNA in the differentiation and evolution of sex chromosomes in A. fraterculus sp. 1, shedding new light on the origin and diversification of Anastrepha species in South America and providing valuable cytogenetic insights for future research.
Instituto de Genética
Fil: Giardini, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina
Fil: Giardini, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Giardini, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Milla, Fabian Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina
Fil: Milla, Fabian Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Milla, Fabian Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina
Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Conte, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nieves, Mariela. Dirección de investigaciones Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno” (CEMIC). Grupo de estudios en Arquitectura Genómica de Mamíferos (arGENma); Argentina
Fil: Nieves, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Molecular Biology Reports 52 (1) : 250 (Published: 20 February 2025)
Materia
Sex Chromosomes
Hybridization
Cromosomas Sexuales
Anastrepha fraterculus
Hibridación
Molecular Differentiation
Diferenciación Molecular
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21878
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21878
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH)Giardini, Maria CeciliaMilla, Fabian HoracioConte, Claudia AlejandraLanzavecchia, Silvia BeatrizNieves, MarielaSex ChromosomesHybridizationCromosomas SexualesAnastrepha fraterculusHibridaciónMolecular DifferentiationDiferenciación MolecularBackground: The South American fruit fly Anastrepha fraterculus sp. 1 belongs to a cryptic species complex with only a single morphotype present in Argentina, which possess polymorphisms in the sex chromosomes, identified by two X (X1, X2) and two Y (Y5, Y6) variants. Our aim was to explore the molecular composition of the sex chromosomes identified in the laboratory strain Af-Y-short due to it possesses the most frequent sex chromosomes variants, and the smallest Y chromosome found in wild populations and laboratory colonies of A. fraterculus sp. 1. Methods and results: Whole Comparative Genomic Hybridization (W-CGH) was applied to the Af-Y-short laboratory strain to identify potential differences in the size and composition of highly repetitive DNA blocks between individuals, morphs or sexes. Our results showed that the X1 and X2 share complete homology in euchromatic regions, also sharing similar sequences with the Y5 chromosome in the centromeric region. Females X1X1 and X1X2 exhibited repetitive DNA in telomeric regions which contributed to their morphological differentiation, while the Y chromosome evidenced the accumulation of specific and differential repetitive sequences. In contrast, autosomes showed balanced signal with no sex-chromosome-specific sequence accumulation. Conclusions: Our findings highlight the role of repetitive DNA in the differentiation and evolution of sex chromosomes in A. fraterculus sp. 1, shedding new light on the origin and diversification of Anastrepha species in South America and providing valuable cytogenetic insights for future research.Instituto de GenéticaFil: Giardini, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; ArgentinaFil: Giardini, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Giardini, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Milla, Fabian Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; ArgentinaFil: Milla, Fabian Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Milla, Fabian Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; ArgentinaFil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Conte, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; ArgentinaFil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nieves, Mariela. Dirección de investigaciones Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno” (CEMIC). Grupo de estudios en Arquitectura Genómica de Mamíferos (arGENma); ArgentinaFil: Nieves, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaSpringer2025-04-03T11:40:16Z2025-04-03T11:40:16Z2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21878https://link.springer.com/article/10.1007/s11033-025-10277-w1573-4978https://doi.org/10.1007/s11033-025-10277-wMolecular Biology Reports 52 (1) : 250 (Published: 20 February 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2023-PD-L01-I106, Plagas y enfermedades emergentes, cuarentenarias y limitantes en cultivos frutícolas: estrategias para mejorar su prevención, detección precoz y controlinfo:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada.info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:19:28Zoai:localhost:20.500.12123/21878instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:19:28.691INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH)
title Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH)
spellingShingle Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH)
Giardini, Maria Cecilia
Sex Chromosomes
Hybridization
Cromosomas Sexuales
Anastrepha fraterculus
Hibridación
Molecular Differentiation
Diferenciación Molecular
title_short Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH)
title_full Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH)
title_fullStr Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH)
title_full_unstemmed Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH)
title_sort Exploring the molecular differentiation of sex chromosomes in Anastrepha fraterculus sp. 1 using comparative genomic hybridization (CGH)
dc.creator.none.fl_str_mv Giardini, Maria Cecilia
Milla, Fabian Horacio
Conte, Claudia Alejandra
Lanzavecchia, Silvia Beatriz
Nieves, Mariela
author Giardini, Maria Cecilia
author_facet Giardini, Maria Cecilia
Milla, Fabian Horacio
Conte, Claudia Alejandra
Lanzavecchia, Silvia Beatriz
Nieves, Mariela
author_role author
author2 Milla, Fabian Horacio
Conte, Claudia Alejandra
Lanzavecchia, Silvia Beatriz
Nieves, Mariela
author2_role author
author
author
author
dc.subject.none.fl_str_mv Sex Chromosomes
Hybridization
Cromosomas Sexuales
Anastrepha fraterculus
Hibridación
Molecular Differentiation
Diferenciación Molecular
topic Sex Chromosomes
Hybridization
Cromosomas Sexuales
Anastrepha fraterculus
Hibridación
Molecular Differentiation
Diferenciación Molecular
dc.description.none.fl_txt_mv Background: The South American fruit fly Anastrepha fraterculus sp. 1 belongs to a cryptic species complex with only a single morphotype present in Argentina, which possess polymorphisms in the sex chromosomes, identified by two X (X1, X2) and two Y (Y5, Y6) variants. Our aim was to explore the molecular composition of the sex chromosomes identified in the laboratory strain Af-Y-short due to it possesses the most frequent sex chromosomes variants, and the smallest Y chromosome found in wild populations and laboratory colonies of A. fraterculus sp. 1. Methods and results: Whole Comparative Genomic Hybridization (W-CGH) was applied to the Af-Y-short laboratory strain to identify potential differences in the size and composition of highly repetitive DNA blocks between individuals, morphs or sexes. Our results showed that the X1 and X2 share complete homology in euchromatic regions, also sharing similar sequences with the Y5 chromosome in the centromeric region. Females X1X1 and X1X2 exhibited repetitive DNA in telomeric regions which contributed to their morphological differentiation, while the Y chromosome evidenced the accumulation of specific and differential repetitive sequences. In contrast, autosomes showed balanced signal with no sex-chromosome-specific sequence accumulation. Conclusions: Our findings highlight the role of repetitive DNA in the differentiation and evolution of sex chromosomes in A. fraterculus sp. 1, shedding new light on the origin and diversification of Anastrepha species in South America and providing valuable cytogenetic insights for future research.
Instituto de Genética
Fil: Giardini, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina
Fil: Giardini, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Giardini, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Milla, Fabian Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina
Fil: Milla, Fabian Horacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Milla, Fabian Horacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina
Fil: Conte, Claudia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Conte, Claudia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética. Laboratorio de Insectos de Importancia Agronómica; Argentina
Fil: Lanzavecchia, Silvia Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Lanzavecchia, Silvia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nieves, Mariela. Dirección de investigaciones Centro de Educación Médica e Investigaciones Clínicas “Norberto Quirno” (CEMIC). Grupo de estudios en Arquitectura Genómica de Mamíferos (arGENma); Argentina
Fil: Nieves, Mariela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Background: The South American fruit fly Anastrepha fraterculus sp. 1 belongs to a cryptic species complex with only a single morphotype present in Argentina, which possess polymorphisms in the sex chromosomes, identified by two X (X1, X2) and two Y (Y5, Y6) variants. Our aim was to explore the molecular composition of the sex chromosomes identified in the laboratory strain Af-Y-short due to it possesses the most frequent sex chromosomes variants, and the smallest Y chromosome found in wild populations and laboratory colonies of A. fraterculus sp. 1. Methods and results: Whole Comparative Genomic Hybridization (W-CGH) was applied to the Af-Y-short laboratory strain to identify potential differences in the size and composition of highly repetitive DNA blocks between individuals, morphs or sexes. Our results showed that the X1 and X2 share complete homology in euchromatic regions, also sharing similar sequences with the Y5 chromosome in the centromeric region. Females X1X1 and X1X2 exhibited repetitive DNA in telomeric regions which contributed to their morphological differentiation, while the Y chromosome evidenced the accumulation of specific and differential repetitive sequences. In contrast, autosomes showed balanced signal with no sex-chromosome-specific sequence accumulation. Conclusions: Our findings highlight the role of repetitive DNA in the differentiation and evolution of sex chromosomes in A. fraterculus sp. 1, shedding new light on the origin and diversification of Anastrepha species in South America and providing valuable cytogenetic insights for future research.
publishDate 2025
dc.date.none.fl_str_mv 2025-04-03T11:40:16Z
2025-04-03T11:40:16Z
2025-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21878
https://link.springer.com/article/10.1007/s11033-025-10277-w
1573-4978
https://doi.org/10.1007/s11033-025-10277-w
url http://hdl.handle.net/20.500.12123/21878
https://link.springer.com/article/10.1007/s11033-025-10277-w
https://doi.org/10.1007/s11033-025-10277-w
identifier_str_mv 1573-4978
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2023-PD-L01-I106, Plagas y enfermedades emergentes, cuarentenarias y limitantes en cultivos frutícolas: estrategias para mejorar su prevención, detección precoz y control
info:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Molecular Biology Reports 52 (1) : 250 (Published: 20 February 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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