Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina

Autores
Colombo, Denis Nahuel; Figueruelo, Andrea Mariana; Paniego, Norma Beatriz; Comerio, Ricardo Mario; Corro Molas, Andres Ezequiel
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During a survey conducted in the 2021, 2022 and 2023 seasons, sunflower heads showing symptoms of dry rot were collected from 80 fields in the semi-arid Pampa region of Argentina. A total of 72% of the fields examined in 2021, 71% in 2022 and 63% in 2023 showed the presence of the disease. The highest incidence was recorded as 70% in 2021. Phomopsis head rot began as dry, brown spots on the back of the head, progressing to necrosis and twisting of adjacent leaves. Infected head tissue samples were surface sterilised, cultured on potato dextrose agar (PDA) and incubated at 25°C for 14 days. Diaporthe helianthi was identified based on cultural and morphological characteristics as well as molecular data. A phylogenetic analysis was performed. Internal transcribed spacer (ITS), translation elongation factor 1-α (ef1-α) and β-tubulin sequences were deposited in GenBank, showing the identity with the ex-type D. helianthi strain CBS 592.81. Pathogenicity experiments confirmed the presence of similar disease symptoms in inoculated sunflower heads, and D. helianthi was consistently reisolated from these organs. Our Koch's postulates testing results on heads constitute the first confirmed report that D. helianthi is the cause of Phomopsis head rot on sunflower in Argentina.
Instituto de Biotecnología
Fil: Colombo, Denis Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Figueruelo, Andrea Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Figueruelo, Andrea Mariana. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Paniego, Norma Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Comerio, Ricardo Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Corro Molas, Andres Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Corro Molas, Andres Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil. Agencia de Extensión Rural General Pico; Argentina
Fuente
Journal of Phytopathology 172 (5) : e13416 (September/October 2024)
Materia
Disease Recognition
Fungi
Phylogenetic Analysis
Phomopsis
Diaporthe helianthi
Sunflowers
Reconocimiento de la Enfermedad
Hongos
Análisis Filogenético
Girasol
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/20452
Acceder
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oai_identifier_str oai:localhost:20.500.12123/20452
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in ArgentinaColombo, Denis NahuelFigueruelo, Andrea MarianaPaniego, Norma BeatrizComerio, Ricardo MarioCorro Molas, Andres EzequielDisease RecognitionFungiPhylogenetic AnalysisPhomopsisDiaporthe helianthiSunflowersReconocimiento de la EnfermedadHongosAnálisis FilogenéticoGirasolArgentinaDuring a survey conducted in the 2021, 2022 and 2023 seasons, sunflower heads showing symptoms of dry rot were collected from 80 fields in the semi-arid Pampa region of Argentina. A total of 72% of the fields examined in 2021, 71% in 2022 and 63% in 2023 showed the presence of the disease. The highest incidence was recorded as 70% in 2021. Phomopsis head rot began as dry, brown spots on the back of the head, progressing to necrosis and twisting of adjacent leaves. Infected head tissue samples were surface sterilised, cultured on potato dextrose agar (PDA) and incubated at 25°C for 14 days. Diaporthe helianthi was identified based on cultural and morphological characteristics as well as molecular data. A phylogenetic analysis was performed. Internal transcribed spacer (ITS), translation elongation factor 1-α (ef1-α) and β-tubulin sequences were deposited in GenBank, showing the identity with the ex-type D. helianthi strain CBS 592.81. Pathogenicity experiments confirmed the presence of similar disease symptoms in inoculated sunflower heads, and D. helianthi was consistently reisolated from these organs. Our Koch's postulates testing results on heads constitute the first confirmed report that D. helianthi is the cause of Phomopsis head rot on sunflower in Argentina.Instituto de BiotecnologíaFil: Colombo, Denis Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; ArgentinaFil: Figueruelo, Andrea Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; ArgentinaFil: Figueruelo, Andrea Mariana. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Paniego, Norma Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Comerio, Ricardo Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; ArgentinaFil: Corro Molas, Andres Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Corro Molas, Andres Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil. Agencia de Extensión Rural General Pico; ArgentinaWiley2024-12-03T13:39:09Z2024-12-03T13:39:09Z2024-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/20452https://onlinelibrary.wiley.com/doi/10.1111/jph.134161439-0434https://doi.org/10.1111/jph.13416Journal of Phytopathology 172 (5) : e13416 (September/October 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E4-I090-001, Análisis de patosistemas en cultivos agrícolas y especies forestales. Caracterización de sus componentesinfo:eu-repograntAgreement/INTA/2019-PE-E6-I114-001, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicadainfo:eu-repograntAgreement/INTA/2023-PE-L01-I111, Mejoramiento genético de oleaginosas: girasol, soja, colza y lino en rendimiento, calidad y sanidad, para contribuir a la sostenibilidad de los sistemas productivosinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:47Zoai:localhost:20.500.12123/20452instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:47.638INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina
title Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina
spellingShingle Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina
Colombo, Denis Nahuel
Disease Recognition
Fungi
Phylogenetic Analysis
Phomopsis
Diaporthe helianthi
Sunflowers
Reconocimiento de la Enfermedad
Hongos
Análisis Filogenético
Girasol
Argentina
title_short Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina
title_full Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina
title_fullStr Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina
title_full_unstemmed Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina
title_sort Phomopsis head rot caused by Diaporthe helianthi : A new disease on sunflower in Argentina
dc.creator.none.fl_str_mv Colombo, Denis Nahuel
Figueruelo, Andrea Mariana
Paniego, Norma Beatriz
Comerio, Ricardo Mario
Corro Molas, Andres Ezequiel
author Colombo, Denis Nahuel
author_facet Colombo, Denis Nahuel
Figueruelo, Andrea Mariana
Paniego, Norma Beatriz
Comerio, Ricardo Mario
Corro Molas, Andres Ezequiel
author_role author
author2 Figueruelo, Andrea Mariana
Paniego, Norma Beatriz
Comerio, Ricardo Mario
Corro Molas, Andres Ezequiel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Disease Recognition
Fungi
Phylogenetic Analysis
Phomopsis
Diaporthe helianthi
Sunflowers
Reconocimiento de la Enfermedad
Hongos
Análisis Filogenético
Girasol
Argentina
topic Disease Recognition
Fungi
Phylogenetic Analysis
Phomopsis
Diaporthe helianthi
Sunflowers
Reconocimiento de la Enfermedad
Hongos
Análisis Filogenético
Girasol
Argentina
dc.description.none.fl_txt_mv During a survey conducted in the 2021, 2022 and 2023 seasons, sunflower heads showing symptoms of dry rot were collected from 80 fields in the semi-arid Pampa region of Argentina. A total of 72% of the fields examined in 2021, 71% in 2022 and 63% in 2023 showed the presence of the disease. The highest incidence was recorded as 70% in 2021. Phomopsis head rot began as dry, brown spots on the back of the head, progressing to necrosis and twisting of adjacent leaves. Infected head tissue samples were surface sterilised, cultured on potato dextrose agar (PDA) and incubated at 25°C for 14 days. Diaporthe helianthi was identified based on cultural and morphological characteristics as well as molecular data. A phylogenetic analysis was performed. Internal transcribed spacer (ITS), translation elongation factor 1-α (ef1-α) and β-tubulin sequences were deposited in GenBank, showing the identity with the ex-type D. helianthi strain CBS 592.81. Pathogenicity experiments confirmed the presence of similar disease symptoms in inoculated sunflower heads, and D. helianthi was consistently reisolated from these organs. Our Koch's postulates testing results on heads constitute the first confirmed report that D. helianthi is the cause of Phomopsis head rot on sunflower in Argentina.
Instituto de Biotecnología
Fil: Colombo, Denis Nahuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Figueruelo, Andrea Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Figueruelo, Andrea Mariana. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Paniego, Norma Beatriz. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Paniego, Norma Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Comerio, Ricardo Mario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Corro Molas, Andres Ezequiel. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Corro Molas, Andres Ezequiel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil. Agencia de Extensión Rural General Pico; Argentina
description During a survey conducted in the 2021, 2022 and 2023 seasons, sunflower heads showing symptoms of dry rot were collected from 80 fields in the semi-arid Pampa region of Argentina. A total of 72% of the fields examined in 2021, 71% in 2022 and 63% in 2023 showed the presence of the disease. The highest incidence was recorded as 70% in 2021. Phomopsis head rot began as dry, brown spots on the back of the head, progressing to necrosis and twisting of adjacent leaves. Infected head tissue samples were surface sterilised, cultured on potato dextrose agar (PDA) and incubated at 25°C for 14 days. Diaporthe helianthi was identified based on cultural and morphological characteristics as well as molecular data. A phylogenetic analysis was performed. Internal transcribed spacer (ITS), translation elongation factor 1-α (ef1-α) and β-tubulin sequences were deposited in GenBank, showing the identity with the ex-type D. helianthi strain CBS 592.81. Pathogenicity experiments confirmed the presence of similar disease symptoms in inoculated sunflower heads, and D. helianthi was consistently reisolated from these organs. Our Koch's postulates testing results on heads constitute the first confirmed report that D. helianthi is the cause of Phomopsis head rot on sunflower in Argentina.
publishDate 2024
dc.date.none.fl_str_mv 2024-12-03T13:39:09Z
2024-12-03T13:39:09Z
2024-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/20452
https://onlinelibrary.wiley.com/doi/10.1111/jph.13416
1439-0434
https://doi.org/10.1111/jph.13416
url http://hdl.handle.net/20.500.12123/20452
https://onlinelibrary.wiley.com/doi/10.1111/jph.13416
https://doi.org/10.1111/jph.13416
identifier_str_mv 1439-0434
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E4-I090-001, Análisis de patosistemas en cultivos agrícolas y especies forestales. Caracterización de sus componentes
info:eu-repograntAgreement/INTA/2019-PE-E6-I114-001, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada
info:eu-repograntAgreement/INTA/2023-PE-L01-I111, Mejoramiento genético de oleaginosas: girasol, soja, colza y lino en rendimiento, calidad y sanidad, para contribuir a la sostenibilidad de los sistemas productivos
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Journal of Phytopathology 172 (5) : e13416 (September/October 2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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