Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America

Autores
San Blas, Germán; Baudino, Estela Maris; Silva Dias, Fernando Maia; Dolibaina, Diego Rodrigo; Specht, Alexandre; Martins Casagrande, Mirna; Cornejo, Paula; Guillot Giraudo, Walter; Hendrik Mielke, Olaf Hermann
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Accurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countries of South America, species of noctuids with available sequences are scarce, mostly restricted to species of economic importance, making molecular identification untenable. Here we sequenced the mitochondrial DNA ‘barcode’ region of the cytochrome c oxidase subunit I gene of 34 specimens pertaining to 26 species of South American noctuids, 20 of these for the first time. For all species, genetic distances were higher for interspecific than for intraspecific relations, supporting the utility of DNA ‘barcodes’ to identify species. Larger intraspecific divergences occurred in species of Agrotis and Anicla, and interspecific divergences lower than 2% occurred in about one fifth of the species, all in species of Agrotis, Feltia, and Anicla. These results will allow identifying these species using DNA ‘barcodes’, either for pest management or general biological studies. Furthermore, we carried out phylogenetic analyses with those sequences and 158 other sequences of 85 species of noctuids mined from GenBank. These analyses, in every case, grouped species of the same genus suggesting that the DNA ‘barcodes’ region alone can be useful for lower level phylogeny in this group, recovering as monophyletic groups such as Copitarsia, Agrotis and Austrandesiita. Conversely, groups such as Mythimna (Mythimna) and the subgenera of Feltia were not recovered as monophyletic, suggesting the need for further taxonomic studies in these groups.
EEA Anguil
Fil: San Blas, Diego German. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Baudino, Estela Maris. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Silva Dias, Fernando Maia. Universidade Federal do Paraná; Brasil. Universidade Estadual de Londrina; Brasil
Fil: Dolibaina, Diego Rodrigo. Universidade Federal do Paraná; Brasil
Fil: Specht, Alexandre. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Casagrande, Mirna Martins. Universidade Federal do Paraná; Brasil
Fil: Cornejo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Guillot Giraudo, Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Hendrik Mielke, Olaf Hermann. Universidade Federal do Paraná; Brasil
Fuente
Revista Brasileira de Entomologia 65 (4) : 1-14 (2021)
Materia
Agrotis
Feltia
Mythimna
Copitarsia
Neotropical
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15676
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oai_identifier_str oai:localhost:20.500.12123/15676
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network_name_str INTA Digital (INTA)
spelling Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South AmericaSan Blas, GermánBaudino, Estela MarisSilva Dias, Fernando MaiaDolibaina, Diego RodrigoSpecht, AlexandreMartins Casagrande, MirnaCornejo, PaulaGuillot Giraudo, WalterHendrik Mielke, Olaf HermannAgrotisFeltiaMythimnaCopitarsiaNeotropicalAccurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countries of South America, species of noctuids with available sequences are scarce, mostly restricted to species of economic importance, making molecular identification untenable. Here we sequenced the mitochondrial DNA ‘barcode’ region of the cytochrome c oxidase subunit I gene of 34 specimens pertaining to 26 species of South American noctuids, 20 of these for the first time. For all species, genetic distances were higher for interspecific than for intraspecific relations, supporting the utility of DNA ‘barcodes’ to identify species. Larger intraspecific divergences occurred in species of Agrotis and Anicla, and interspecific divergences lower than 2% occurred in about one fifth of the species, all in species of Agrotis, Feltia, and Anicla. These results will allow identifying these species using DNA ‘barcodes’, either for pest management or general biological studies. Furthermore, we carried out phylogenetic analyses with those sequences and 158 other sequences of 85 species of noctuids mined from GenBank. These analyses, in every case, grouped species of the same genus suggesting that the DNA ‘barcodes’ region alone can be useful for lower level phylogeny in this group, recovering as monophyletic groups such as Copitarsia, Agrotis and Austrandesiita. Conversely, groups such as Mythimna (Mythimna) and the subgenera of Feltia were not recovered as monophyletic, suggesting the need for further taxonomic studies in these groups.EEA AnguilFil: San Blas, Diego German. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Baudino, Estela Maris. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Silva Dias, Fernando Maia. Universidade Federal do Paraná; Brasil. Universidade Estadual de Londrina; BrasilFil: Dolibaina, Diego Rodrigo. Universidade Federal do Paraná; BrasilFil: Specht, Alexandre. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Casagrande, Mirna Martins. Universidade Federal do Paraná; BrasilFil: Cornejo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaFil: Guillot Giraudo, Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; ArgentinaFil: Hendrik Mielke, Olaf Hermann. Universidade Federal do Paraná; BrasilSociedade Brasileira de Entomologia2023-10-25T13:01:08Z2023-10-25T13:01:08Z2021-12-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15676https://www.scielo.br/j/rbent/a/X6jrcFTN3XsBChPfxyWzYVj/?lang=en1806-96650085-5626https://doi.org/10.1590/1806-9665-RBENT-2021-0104Revista Brasileira de Entomologia 65 (4) : 1-14 (2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:20Zoai:localhost:20.500.12123/15676instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:21.219INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America
title Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America
spellingShingle Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America
San Blas, Germán
Agrotis
Feltia
Mythimna
Copitarsia
Neotropical
title_short Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America
title_full Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America
title_fullStr Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America
title_full_unstemmed Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America
title_sort Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America
dc.creator.none.fl_str_mv San Blas, Germán
Baudino, Estela Maris
Silva Dias, Fernando Maia
Dolibaina, Diego Rodrigo
Specht, Alexandre
Martins Casagrande, Mirna
Cornejo, Paula
Guillot Giraudo, Walter
Hendrik Mielke, Olaf Hermann
author San Blas, Germán
author_facet San Blas, Germán
Baudino, Estela Maris
Silva Dias, Fernando Maia
Dolibaina, Diego Rodrigo
Specht, Alexandre
Martins Casagrande, Mirna
Cornejo, Paula
Guillot Giraudo, Walter
Hendrik Mielke, Olaf Hermann
author_role author
author2 Baudino, Estela Maris
Silva Dias, Fernando Maia
Dolibaina, Diego Rodrigo
Specht, Alexandre
Martins Casagrande, Mirna
Cornejo, Paula
Guillot Giraudo, Walter
Hendrik Mielke, Olaf Hermann
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Agrotis
Feltia
Mythimna
Copitarsia
Neotropical
topic Agrotis
Feltia
Mythimna
Copitarsia
Neotropical
dc.description.none.fl_txt_mv Accurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countries of South America, species of noctuids with available sequences are scarce, mostly restricted to species of economic importance, making molecular identification untenable. Here we sequenced the mitochondrial DNA ‘barcode’ region of the cytochrome c oxidase subunit I gene of 34 specimens pertaining to 26 species of South American noctuids, 20 of these for the first time. For all species, genetic distances were higher for interspecific than for intraspecific relations, supporting the utility of DNA ‘barcodes’ to identify species. Larger intraspecific divergences occurred in species of Agrotis and Anicla, and interspecific divergences lower than 2% occurred in about one fifth of the species, all in species of Agrotis, Feltia, and Anicla. These results will allow identifying these species using DNA ‘barcodes’, either for pest management or general biological studies. Furthermore, we carried out phylogenetic analyses with those sequences and 158 other sequences of 85 species of noctuids mined from GenBank. These analyses, in every case, grouped species of the same genus suggesting that the DNA ‘barcodes’ region alone can be useful for lower level phylogeny in this group, recovering as monophyletic groups such as Copitarsia, Agrotis and Austrandesiita. Conversely, groups such as Mythimna (Mythimna) and the subgenera of Feltia were not recovered as monophyletic, suggesting the need for further taxonomic studies in these groups.
EEA Anguil
Fil: San Blas, Diego German. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Baudino, Estela Maris. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Silva Dias, Fernando Maia. Universidade Federal do Paraná; Brasil. Universidade Estadual de Londrina; Brasil
Fil: Dolibaina, Diego Rodrigo. Universidade Federal do Paraná; Brasil
Fil: Specht, Alexandre. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Casagrande, Mirna Martins. Universidade Federal do Paraná; Brasil
Fil: Cornejo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Guillot Giraudo, Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Hendrik Mielke, Olaf Hermann. Universidade Federal do Paraná; Brasil
description Accurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countries of South America, species of noctuids with available sequences are scarce, mostly restricted to species of economic importance, making molecular identification untenable. Here we sequenced the mitochondrial DNA ‘barcode’ region of the cytochrome c oxidase subunit I gene of 34 specimens pertaining to 26 species of South American noctuids, 20 of these for the first time. For all species, genetic distances were higher for interspecific than for intraspecific relations, supporting the utility of DNA ‘barcodes’ to identify species. Larger intraspecific divergences occurred in species of Agrotis and Anicla, and interspecific divergences lower than 2% occurred in about one fifth of the species, all in species of Agrotis, Feltia, and Anicla. These results will allow identifying these species using DNA ‘barcodes’, either for pest management or general biological studies. Furthermore, we carried out phylogenetic analyses with those sequences and 158 other sequences of 85 species of noctuids mined from GenBank. These analyses, in every case, grouped species of the same genus suggesting that the DNA ‘barcodes’ region alone can be useful for lower level phylogeny in this group, recovering as monophyletic groups such as Copitarsia, Agrotis and Austrandesiita. Conversely, groups such as Mythimna (Mythimna) and the subgenera of Feltia were not recovered as monophyletic, suggesting the need for further taxonomic studies in these groups.
publishDate 2021
dc.date.none.fl_str_mv 2021-12-13
2023-10-25T13:01:08Z
2023-10-25T13:01:08Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15676
https://www.scielo.br/j/rbent/a/X6jrcFTN3XsBChPfxyWzYVj/?lang=en
1806-9665
0085-5626
https://doi.org/10.1590/1806-9665-RBENT-2021-0104
url http://hdl.handle.net/20.500.12123/15676
https://www.scielo.br/j/rbent/a/X6jrcFTN3XsBChPfxyWzYVj/?lang=en
https://doi.org/10.1590/1806-9665-RBENT-2021-0104
identifier_str_mv 1806-9665
0085-5626
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira de Entomologia
publisher.none.fl_str_mv Sociedade Brasileira de Entomologia
dc.source.none.fl_str_mv Revista Brasileira de Entomologia 65 (4) : 1-14 (2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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