Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America
- Autores
- San Blas, Germán; Baudino, Estela Maris; Silva Dias, Fernando Maia; Dolibaina, Diego Rodrigo; Specht, Alexandre; Martins Casagrande, Mirna; Cornejo, Paula; Guillot Giraudo, Walter; Hendrik Mielke, Olaf Hermann
- Año de publicación
- 2021
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Accurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countries of South America, species of noctuids with available sequences are scarce, mostly restricted to species of economic importance, making molecular identification untenable. Here we sequenced the mitochondrial DNA ‘barcode’ region of the cytochrome c oxidase subunit I gene of 34 specimens pertaining to 26 species of South American noctuids, 20 of these for the first time. For all species, genetic distances were higher for interspecific than for intraspecific relations, supporting the utility of DNA ‘barcodes’ to identify species. Larger intraspecific divergences occurred in species of Agrotis and Anicla, and interspecific divergences lower than 2% occurred in about one fifth of the species, all in species of Agrotis, Feltia, and Anicla. These results will allow identifying these species using DNA ‘barcodes’, either for pest management or general biological studies. Furthermore, we carried out phylogenetic analyses with those sequences and 158 other sequences of 85 species of noctuids mined from GenBank. These analyses, in every case, grouped species of the same genus suggesting that the DNA ‘barcodes’ region alone can be useful for lower level phylogeny in this group, recovering as monophyletic groups such as Copitarsia, Agrotis and Austrandesiita. Conversely, groups such as Mythimna (Mythimna) and the subgenera of Feltia were not recovered as monophyletic, suggesting the need for further taxonomic studies in these groups.
EEA Anguil
Fil: San Blas, Diego German. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina
Fil: Baudino, Estela Maris. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina
Fil: Silva Dias, Fernando Maia. Universidade Federal do Paraná; Brasil. Universidade Estadual de Londrina; Brasil
Fil: Dolibaina, Diego Rodrigo. Universidade Federal do Paraná; Brasil
Fil: Specht, Alexandre. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil
Fil: Casagrande, Mirna Martins. Universidade Federal do Paraná; Brasil
Fil: Cornejo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina
Fil: Guillot Giraudo, Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Hendrik Mielke, Olaf Hermann. Universidade Federal do Paraná; Brasil - Fuente
- Revista Brasileira de Entomologia 65 (4) : 1-14 (2021)
- Materia
-
Agrotis
Feltia
Mythimna
Copitarsia
Neotropical - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/15676
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Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South AmericaSan Blas, GermánBaudino, Estela MarisSilva Dias, Fernando MaiaDolibaina, Diego RodrigoSpecht, AlexandreMartins Casagrande, MirnaCornejo, PaulaGuillot Giraudo, WalterHendrik Mielke, Olaf HermannAgrotisFeltiaMythimnaCopitarsiaNeotropicalAccurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countries of South America, species of noctuids with available sequences are scarce, mostly restricted to species of economic importance, making molecular identification untenable. Here we sequenced the mitochondrial DNA ‘barcode’ region of the cytochrome c oxidase subunit I gene of 34 specimens pertaining to 26 species of South American noctuids, 20 of these for the first time. For all species, genetic distances were higher for interspecific than for intraspecific relations, supporting the utility of DNA ‘barcodes’ to identify species. Larger intraspecific divergences occurred in species of Agrotis and Anicla, and interspecific divergences lower than 2% occurred in about one fifth of the species, all in species of Agrotis, Feltia, and Anicla. These results will allow identifying these species using DNA ‘barcodes’, either for pest management or general biological studies. Furthermore, we carried out phylogenetic analyses with those sequences and 158 other sequences of 85 species of noctuids mined from GenBank. These analyses, in every case, grouped species of the same genus suggesting that the DNA ‘barcodes’ region alone can be useful for lower level phylogeny in this group, recovering as monophyletic groups such as Copitarsia, Agrotis and Austrandesiita. Conversely, groups such as Mythimna (Mythimna) and the subgenera of Feltia were not recovered as monophyletic, suggesting the need for further taxonomic studies in these groups.EEA AnguilFil: San Blas, Diego German. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; ArgentinaFil: Baudino, Estela Maris. Universidad Nacional de La Pampa. Facultad de Agronomía; ArgentinaFil: Silva Dias, Fernando Maia. Universidade Federal do Paraná; Brasil. Universidade Estadual de Londrina; BrasilFil: Dolibaina, Diego Rodrigo. Universidade Federal do Paraná; BrasilFil: Specht, Alexandre. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; BrasilFil: Casagrande, Mirna Martins. Universidade Federal do Paraná; BrasilFil: Cornejo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; ArgentinaFil: Guillot Giraudo, Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; ArgentinaFil: Hendrik Mielke, Olaf Hermann. Universidade Federal do Paraná; BrasilSociedade Brasileira de Entomologia2023-10-25T13:01:08Z2023-10-25T13:01:08Z2021-12-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15676https://www.scielo.br/j/rbent/a/X6jrcFTN3XsBChPfxyWzYVj/?lang=en1806-96650085-5626https://doi.org/10.1590/1806-9665-RBENT-2021-0104Revista Brasileira de Entomologia 65 (4) : 1-14 (2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:20Zoai:localhost:20.500.12123/15676instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:21.219INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America |
title |
Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America |
spellingShingle |
Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America San Blas, Germán Agrotis Feltia Mythimna Copitarsia Neotropical |
title_short |
Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America |
title_full |
Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America |
title_fullStr |
Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America |
title_full_unstemmed |
Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America |
title_sort |
Molecular characterization and phylogenetic assessment of agricultural-related noctuids (Lepidoptera: Noctuidae) of South America |
dc.creator.none.fl_str_mv |
San Blas, Germán Baudino, Estela Maris Silva Dias, Fernando Maia Dolibaina, Diego Rodrigo Specht, Alexandre Martins Casagrande, Mirna Cornejo, Paula Guillot Giraudo, Walter Hendrik Mielke, Olaf Hermann |
author |
San Blas, Germán |
author_facet |
San Blas, Germán Baudino, Estela Maris Silva Dias, Fernando Maia Dolibaina, Diego Rodrigo Specht, Alexandre Martins Casagrande, Mirna Cornejo, Paula Guillot Giraudo, Walter Hendrik Mielke, Olaf Hermann |
author_role |
author |
author2 |
Baudino, Estela Maris Silva Dias, Fernando Maia Dolibaina, Diego Rodrigo Specht, Alexandre Martins Casagrande, Mirna Cornejo, Paula Guillot Giraudo, Walter Hendrik Mielke, Olaf Hermann |
author2_role |
author author author author author author author author |
dc.subject.none.fl_str_mv |
Agrotis Feltia Mythimna Copitarsia Neotropical |
topic |
Agrotis Feltia Mythimna Copitarsia Neotropical |
dc.description.none.fl_txt_mv |
Accurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countries of South America, species of noctuids with available sequences are scarce, mostly restricted to species of economic importance, making molecular identification untenable. Here we sequenced the mitochondrial DNA ‘barcode’ region of the cytochrome c oxidase subunit I gene of 34 specimens pertaining to 26 species of South American noctuids, 20 of these for the first time. For all species, genetic distances were higher for interspecific than for intraspecific relations, supporting the utility of DNA ‘barcodes’ to identify species. Larger intraspecific divergences occurred in species of Agrotis and Anicla, and interspecific divergences lower than 2% occurred in about one fifth of the species, all in species of Agrotis, Feltia, and Anicla. These results will allow identifying these species using DNA ‘barcodes’, either for pest management or general biological studies. Furthermore, we carried out phylogenetic analyses with those sequences and 158 other sequences of 85 species of noctuids mined from GenBank. These analyses, in every case, grouped species of the same genus suggesting that the DNA ‘barcodes’ region alone can be useful for lower level phylogeny in this group, recovering as monophyletic groups such as Copitarsia, Agrotis and Austrandesiita. Conversely, groups such as Mythimna (Mythimna) and the subgenera of Feltia were not recovered as monophyletic, suggesting the need for further taxonomic studies in these groups. EEA Anguil Fil: San Blas, Diego German. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina Fil: Baudino, Estela Maris. Universidad Nacional de La Pampa. Facultad de Agronomía; Argentina Fil: Silva Dias, Fernando Maia. Universidade Federal do Paraná; Brasil. Universidade Estadual de Londrina; Brasil Fil: Dolibaina, Diego Rodrigo. Universidade Federal do Paraná; Brasil Fil: Specht, Alexandre. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Casagrande, Mirna Martins. Universidade Federal do Paraná; Brasil Fil: Cornejo, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Provincia de Mendoza. Instituto Argentino de Investigaciones de las Zonas Áridas. Universidad Nacional de Cuyo. Instituto Argentino de Investigaciones de las Zonas Áridas; Argentina Fil: Guillot Giraudo, Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina Fil: Hendrik Mielke, Olaf Hermann. Universidade Federal do Paraná; Brasil |
description |
Accurate identification of species is fundamental to every biological research. While morphological identification is a time-consuming and skilled technique, straightforward molecular techniques require the availability of a database of previously sequenced and identified specimens. For most countries of South America, species of noctuids with available sequences are scarce, mostly restricted to species of economic importance, making molecular identification untenable. Here we sequenced the mitochondrial DNA ‘barcode’ region of the cytochrome c oxidase subunit I gene of 34 specimens pertaining to 26 species of South American noctuids, 20 of these for the first time. For all species, genetic distances were higher for interspecific than for intraspecific relations, supporting the utility of DNA ‘barcodes’ to identify species. Larger intraspecific divergences occurred in species of Agrotis and Anicla, and interspecific divergences lower than 2% occurred in about one fifth of the species, all in species of Agrotis, Feltia, and Anicla. These results will allow identifying these species using DNA ‘barcodes’, either for pest management or general biological studies. Furthermore, we carried out phylogenetic analyses with those sequences and 158 other sequences of 85 species of noctuids mined from GenBank. These analyses, in every case, grouped species of the same genus suggesting that the DNA ‘barcodes’ region alone can be useful for lower level phylogeny in this group, recovering as monophyletic groups such as Copitarsia, Agrotis and Austrandesiita. Conversely, groups such as Mythimna (Mythimna) and the subgenera of Feltia were not recovered as monophyletic, suggesting the need for further taxonomic studies in these groups. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-13 2023-10-25T13:01:08Z 2023-10-25T13:01:08Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/15676 https://www.scielo.br/j/rbent/a/X6jrcFTN3XsBChPfxyWzYVj/?lang=en 1806-9665 0085-5626 https://doi.org/10.1590/1806-9665-RBENT-2021-0104 |
url |
http://hdl.handle.net/20.500.12123/15676 https://www.scielo.br/j/rbent/a/X6jrcFTN3XsBChPfxyWzYVj/?lang=en https://doi.org/10.1590/1806-9665-RBENT-2021-0104 |
identifier_str_mv |
1806-9665 0085-5626 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Entomologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Entomologia |
dc.source.none.fl_str_mv |
Revista Brasileira de Entomologia 65 (4) : 1-14 (2021) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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