Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains

Autores
Abatángelo, Virginia; Peressutti Bacci, Natalia; Boncompain, Carina A.; Amadio, Ariel; Carrasco, Soledad; Suárez, Cristian A.; Morbidoni, Héctor R.
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Staphylococcus aureus is a very successful opportunistic pathogen capable of causing a variety of diseases ranging from mild skin infections to life-threatening sepsis, meningitis and pneumonia. Its ability to display numerous virulence mechanisms matches its skill to display resistance to several antibiotics, including β-lactams, underscoring the fact that new anti-S. aureus drugs are urgently required. In this scenario, the utilization of lytic bacteriophages that kill bacteria in a genus -or even species- specific way, has become an attractive field of study. In this report, we describe the isolation, characterization and sequencing of phages capable of killing S. aureus including methicillin resistant (MRSA) and multi-drug resistant S. aureus local strains from environmental, animal and human origin. Genome sequencing and bio-informatics analysis showed the absence of genes encoding virulence factors, toxins or antibiotic resistance determinants. Of note, there was a high similarity between our set of phages to others described in the literature such as phage K. Considering that reported phages were obtained in different continents, it seems plausible that there is a commonality of genetic features that are needed for optimum, broad host range anti-staphylococcal activity of these related phages. Importantly, the high activity and broad host range of one of our phages underscores its promising value to control the presence of S. aureus in fomites, industry and hospital environments and eventually on animal and human skin. The development of a cocktail of the reported lytic phages active against S. aureus–currently under way- is thus, a sensible strategy against this pathogen.
EEA Rafaela
Fil: Abatángelo, Virginia. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentina
Fil: Peressutti Bacci, Natalia. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentina
Fil: Boncompain, Carina A. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentina
Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina.
Fil: Carrasco, Soledad. Universidad Nacional de Rosario. Bioinformatics Program; Argentina
Fil: Suárez, Cristian A. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentina
Fil: Morbidoni, Héctor R. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentina
Fuente
PLoS ONE 12 (7) : e0181671 (July 2017)
Materia
Staphylococcus aureus
Bacteriófagos
Vectores Genéticos
Secuencia Nucleotídica
Bacteriophages
Genetic Vectors
Nucleotide Sequence
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4752
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4752
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network_name_str INTA Digital (INTA)
spelling Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strainsAbatángelo, VirginiaPeressutti Bacci, NataliaBoncompain, Carina A.Amadio, ArielCarrasco, SoledadSuárez, Cristian A.Morbidoni, Héctor R.Staphylococcus aureusBacteriófagosVectores GenéticosSecuencia NucleotídicaBacteriophagesGenetic VectorsNucleotide SequenceStaphylococcus aureus is a very successful opportunistic pathogen capable of causing a variety of diseases ranging from mild skin infections to life-threatening sepsis, meningitis and pneumonia. Its ability to display numerous virulence mechanisms matches its skill to display resistance to several antibiotics, including β-lactams, underscoring the fact that new anti-S. aureus drugs are urgently required. In this scenario, the utilization of lytic bacteriophages that kill bacteria in a genus -or even species- specific way, has become an attractive field of study. In this report, we describe the isolation, characterization and sequencing of phages capable of killing S. aureus including methicillin resistant (MRSA) and multi-drug resistant S. aureus local strains from environmental, animal and human origin. Genome sequencing and bio-informatics analysis showed the absence of genes encoding virulence factors, toxins or antibiotic resistance determinants. Of note, there was a high similarity between our set of phages to others described in the literature such as phage K. Considering that reported phages were obtained in different continents, it seems plausible that there is a commonality of genetic features that are needed for optimum, broad host range anti-staphylococcal activity of these related phages. Importantly, the high activity and broad host range of one of our phages underscores its promising value to control the presence of S. aureus in fomites, industry and hospital environments and eventually on animal and human skin. The development of a cocktail of the reported lytic phages active against S. aureus–currently under way- is thus, a sensible strategy against this pathogen.EEA RafaelaFil: Abatángelo, Virginia. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; ArgentinaFil: Peressutti Bacci, Natalia. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; ArgentinaFil: Boncompain, Carina A. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; ArgentinaFil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina.Fil: Carrasco, Soledad. Universidad Nacional de Rosario. Bioinformatics Program; ArgentinaFil: Suárez, Cristian A. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; ArgentinaFil: Morbidoni, Héctor R. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; ArgentinaPlos One2019-03-27T12:16:10Z2019-03-27T12:16:10Z2017-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0181671http://hdl.handle.net/20.500.12123/47521932-6203https://doi.org/10.1371/journal.pone.0181671PLoS ONE 12 (7) : e0181671 (July 2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:37Zoai:localhost:20.500.12123/4752instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:37.427INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains
title Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains
spellingShingle Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains
Abatángelo, Virginia
Staphylococcus aureus
Bacteriófagos
Vectores Genéticos
Secuencia Nucleotídica
Bacteriophages
Genetic Vectors
Nucleotide Sequence
title_short Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains
title_full Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains
title_fullStr Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains
title_full_unstemmed Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains
title_sort Broad-range lytic bacteriophages that kill Staphylococcus aureus local field strains
dc.creator.none.fl_str_mv Abatángelo, Virginia
Peressutti Bacci, Natalia
Boncompain, Carina A.
Amadio, Ariel
Carrasco, Soledad
Suárez, Cristian A.
Morbidoni, Héctor R.
author Abatángelo, Virginia
author_facet Abatángelo, Virginia
Peressutti Bacci, Natalia
Boncompain, Carina A.
Amadio, Ariel
Carrasco, Soledad
Suárez, Cristian A.
Morbidoni, Héctor R.
author_role author
author2 Peressutti Bacci, Natalia
Boncompain, Carina A.
Amadio, Ariel
Carrasco, Soledad
Suárez, Cristian A.
Morbidoni, Héctor R.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Staphylococcus aureus
Bacteriófagos
Vectores Genéticos
Secuencia Nucleotídica
Bacteriophages
Genetic Vectors
Nucleotide Sequence
topic Staphylococcus aureus
Bacteriófagos
Vectores Genéticos
Secuencia Nucleotídica
Bacteriophages
Genetic Vectors
Nucleotide Sequence
dc.description.none.fl_txt_mv Staphylococcus aureus is a very successful opportunistic pathogen capable of causing a variety of diseases ranging from mild skin infections to life-threatening sepsis, meningitis and pneumonia. Its ability to display numerous virulence mechanisms matches its skill to display resistance to several antibiotics, including β-lactams, underscoring the fact that new anti-S. aureus drugs are urgently required. In this scenario, the utilization of lytic bacteriophages that kill bacteria in a genus -or even species- specific way, has become an attractive field of study. In this report, we describe the isolation, characterization and sequencing of phages capable of killing S. aureus including methicillin resistant (MRSA) and multi-drug resistant S. aureus local strains from environmental, animal and human origin. Genome sequencing and bio-informatics analysis showed the absence of genes encoding virulence factors, toxins or antibiotic resistance determinants. Of note, there was a high similarity between our set of phages to others described in the literature such as phage K. Considering that reported phages were obtained in different continents, it seems plausible that there is a commonality of genetic features that are needed for optimum, broad host range anti-staphylococcal activity of these related phages. Importantly, the high activity and broad host range of one of our phages underscores its promising value to control the presence of S. aureus in fomites, industry and hospital environments and eventually on animal and human skin. The development of a cocktail of the reported lytic phages active against S. aureus–currently under way- is thus, a sensible strategy against this pathogen.
EEA Rafaela
Fil: Abatángelo, Virginia. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentina
Fil: Peressutti Bacci, Natalia. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentina
Fil: Boncompain, Carina A. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentina
Fil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina.
Fil: Carrasco, Soledad. Universidad Nacional de Rosario. Bioinformatics Program; Argentina
Fil: Suárez, Cristian A. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentina
Fil: Morbidoni, Héctor R. Universidad Nacional de Rosario. Facultad de Ciencias Médicas. Laboratorio de Microbiología Molecular; Argentina
description Staphylococcus aureus is a very successful opportunistic pathogen capable of causing a variety of diseases ranging from mild skin infections to life-threatening sepsis, meningitis and pneumonia. Its ability to display numerous virulence mechanisms matches its skill to display resistance to several antibiotics, including β-lactams, underscoring the fact that new anti-S. aureus drugs are urgently required. In this scenario, the utilization of lytic bacteriophages that kill bacteria in a genus -or even species- specific way, has become an attractive field of study. In this report, we describe the isolation, characterization and sequencing of phages capable of killing S. aureus including methicillin resistant (MRSA) and multi-drug resistant S. aureus local strains from environmental, animal and human origin. Genome sequencing and bio-informatics analysis showed the absence of genes encoding virulence factors, toxins or antibiotic resistance determinants. Of note, there was a high similarity between our set of phages to others described in the literature such as phage K. Considering that reported phages were obtained in different continents, it seems plausible that there is a commonality of genetic features that are needed for optimum, broad host range anti-staphylococcal activity of these related phages. Importantly, the high activity and broad host range of one of our phages underscores its promising value to control the presence of S. aureus in fomites, industry and hospital environments and eventually on animal and human skin. The development of a cocktail of the reported lytic phages active against S. aureus–currently under way- is thus, a sensible strategy against this pathogen.
publishDate 2017
dc.date.none.fl_str_mv 2017-07
2019-03-27T12:16:10Z
2019-03-27T12:16:10Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0181671
http://hdl.handle.net/20.500.12123/4752
1932-6203
https://doi.org/10.1371/journal.pone.0181671
url https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0181671
http://hdl.handle.net/20.500.12123/4752
https://doi.org/10.1371/journal.pone.0181671
identifier_str_mv 1932-6203
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Plos One
publisher.none.fl_str_mv Plos One
dc.source.none.fl_str_mv PLoS ONE 12 (7) : e0181671 (July 2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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