Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina

Autores
Soler, Paula; Abdala, Alejandra Mariana; Larroza, Marcela Patricia
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lymnaeid snails serve as intermediate hosts for Fasciola hepatica (Linnaeus, 1758), the etiological agent of fasciolosis, which is a widespread livestock disease in Argentina. Determining their geographic distribution and identifying the snail species involved in the transmission of fasciolosis can provide crucial information for designing strategic control programs. In this context, this work aimed at genetically characterizing the species of lymnaeid snails collected in different water bodies of northern Patagonia, Argentina. To this end, 689 snails were collected in 12 sites in the provinces of Neuquén, Río Negro and Chubut, in areas where fasciolosis is endemic. According to the morphological characteristics of their valves, they were identified as Galba spp. Twenty-three of these specimens were further identified using the nuclear sequences of the internal transcribed spacers ITS-1 and ITS-2 and 18S rRNA. The results confirmed the identity of all the analyzed snails as Galba viatrix and provided evidence that studying the variable region V2 of the 18S rRNA gene is not enough to differentiate closely related species, as observed in lymnaeid snails. Both the fact that G. viatrix was the only species identified in the endemic area surveyed and previous evidence of the high prevalence of F. hepatica infestation in grazing animals in the region suggest that this species is the main intermediate host of F. hepatica. The correct identification of lymnaeid snail species has great importance to determine risk zones and develop appropriate control measures to reduce transmission, according to the different ecological characteristics of each species.
EEA Bariloche
Fil: Soler, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Salud Animal; Argentina
Fil: Soler, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Soler, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Abdala, Alejandra Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Salud Animal; Argentina
Fil: Larroza, Marcela Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Salud Animal; Argentina
Fuente
Veterinary Parasitology: Regional Studies and Reports 44 : 100919. (September 2023)
Materia
Caracoles
Caracterización Molecular
Distribución Geográfica
Fasciola Hepatica
Snails
Molecular Characterization
Geographical Distribution
Región Patagónica
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15001
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oai_identifier_str oai:localhost:20.500.12123/15001
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spelling Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, ArgentinaSoler, PaulaAbdala, Alejandra MarianaLarroza, Marcela PatriciaCaracolesCaracterización MolecularDistribución GeográficaFasciola HepaticaSnailsMolecular CharacterizationGeographical DistributionRegión PatagónicaLymnaeid snails serve as intermediate hosts for Fasciola hepatica (Linnaeus, 1758), the etiological agent of fasciolosis, which is a widespread livestock disease in Argentina. Determining their geographic distribution and identifying the snail species involved in the transmission of fasciolosis can provide crucial information for designing strategic control programs. In this context, this work aimed at genetically characterizing the species of lymnaeid snails collected in different water bodies of northern Patagonia, Argentina. To this end, 689 snails were collected in 12 sites in the provinces of Neuquén, Río Negro and Chubut, in areas where fasciolosis is endemic. According to the morphological characteristics of their valves, they were identified as Galba spp. Twenty-three of these specimens were further identified using the nuclear sequences of the internal transcribed spacers ITS-1 and ITS-2 and 18S rRNA. The results confirmed the identity of all the analyzed snails as Galba viatrix and provided evidence that studying the variable region V2 of the 18S rRNA gene is not enough to differentiate closely related species, as observed in lymnaeid snails. Both the fact that G. viatrix was the only species identified in the endemic area surveyed and previous evidence of the high prevalence of F. hepatica infestation in grazing animals in the region suggest that this species is the main intermediate host of F. hepatica. The correct identification of lymnaeid snail species has great importance to determine risk zones and develop appropriate control measures to reduce transmission, according to the different ecological characteristics of each species.EEA BarilocheFil: Soler, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Salud Animal; ArgentinaFil: Soler, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Soler, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; ArgentinaFil: Abdala, Alejandra Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Salud Animal; ArgentinaFil: Larroza, Marcela Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Salud Animal; ArgentinaElsevier2023-08-24T11:50:56Z2023-08-24T11:50:56Z2023-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15001https://www.sciencedirect.com/science/article/pii/S24059390230008982405-9390https://doi.org/10.1016/j.vprsr.2023.100919Veterinary Parasitology: Regional Studies and Reports 44 : 100919. (September 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-02-26T11:45:51Zoai:localhost:20.500.12123/15001instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-02-26 11:45:52.014INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina
title Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina
spellingShingle Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina
Soler, Paula
Caracoles
Caracterización Molecular
Distribución Geográfica
Fasciola Hepatica
Snails
Molecular Characterization
Geographical Distribution
Región Patagónica
title_short Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina
title_full Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina
title_fullStr Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina
title_full_unstemmed Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina
title_sort Genetic characterization and regional distribution of lymnaeid snails in northern Patagonia, Argentina
dc.creator.none.fl_str_mv Soler, Paula
Abdala, Alejandra Mariana
Larroza, Marcela Patricia
author Soler, Paula
author_facet Soler, Paula
Abdala, Alejandra Mariana
Larroza, Marcela Patricia
author_role author
author2 Abdala, Alejandra Mariana
Larroza, Marcela Patricia
author2_role author
author
dc.subject.none.fl_str_mv Caracoles
Caracterización Molecular
Distribución Geográfica
Fasciola Hepatica
Snails
Molecular Characterization
Geographical Distribution
Región Patagónica
topic Caracoles
Caracterización Molecular
Distribución Geográfica
Fasciola Hepatica
Snails
Molecular Characterization
Geographical Distribution
Región Patagónica
dc.description.none.fl_txt_mv Lymnaeid snails serve as intermediate hosts for Fasciola hepatica (Linnaeus, 1758), the etiological agent of fasciolosis, which is a widespread livestock disease in Argentina. Determining their geographic distribution and identifying the snail species involved in the transmission of fasciolosis can provide crucial information for designing strategic control programs. In this context, this work aimed at genetically characterizing the species of lymnaeid snails collected in different water bodies of northern Patagonia, Argentina. To this end, 689 snails were collected in 12 sites in the provinces of Neuquén, Río Negro and Chubut, in areas where fasciolosis is endemic. According to the morphological characteristics of their valves, they were identified as Galba spp. Twenty-three of these specimens were further identified using the nuclear sequences of the internal transcribed spacers ITS-1 and ITS-2 and 18S rRNA. The results confirmed the identity of all the analyzed snails as Galba viatrix and provided evidence that studying the variable region V2 of the 18S rRNA gene is not enough to differentiate closely related species, as observed in lymnaeid snails. Both the fact that G. viatrix was the only species identified in the endemic area surveyed and previous evidence of the high prevalence of F. hepatica infestation in grazing animals in the region suggest that this species is the main intermediate host of F. hepatica. The correct identification of lymnaeid snail species has great importance to determine risk zones and develop appropriate control measures to reduce transmission, according to the different ecological characteristics of each species.
EEA Bariloche
Fil: Soler, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Salud Animal; Argentina
Fil: Soler, Paula. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Soler, Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Forestales y Agropecuarias Bariloche; Argentina
Fil: Abdala, Alejandra Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Salud Animal; Argentina
Fil: Larroza, Marcela Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Bariloche. Grupo de Salud Animal; Argentina
description Lymnaeid snails serve as intermediate hosts for Fasciola hepatica (Linnaeus, 1758), the etiological agent of fasciolosis, which is a widespread livestock disease in Argentina. Determining their geographic distribution and identifying the snail species involved in the transmission of fasciolosis can provide crucial information for designing strategic control programs. In this context, this work aimed at genetically characterizing the species of lymnaeid snails collected in different water bodies of northern Patagonia, Argentina. To this end, 689 snails were collected in 12 sites in the provinces of Neuquén, Río Negro and Chubut, in areas where fasciolosis is endemic. According to the morphological characteristics of their valves, they were identified as Galba spp. Twenty-three of these specimens were further identified using the nuclear sequences of the internal transcribed spacers ITS-1 and ITS-2 and 18S rRNA. The results confirmed the identity of all the analyzed snails as Galba viatrix and provided evidence that studying the variable region V2 of the 18S rRNA gene is not enough to differentiate closely related species, as observed in lymnaeid snails. Both the fact that G. viatrix was the only species identified in the endemic area surveyed and previous evidence of the high prevalence of F. hepatica infestation in grazing animals in the region suggest that this species is the main intermediate host of F. hepatica. The correct identification of lymnaeid snail species has great importance to determine risk zones and develop appropriate control measures to reduce transmission, according to the different ecological characteristics of each species.
publishDate 2023
dc.date.none.fl_str_mv 2023-08-24T11:50:56Z
2023-08-24T11:50:56Z
2023-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15001
https://www.sciencedirect.com/science/article/pii/S2405939023000898
2405-9390
https://doi.org/10.1016/j.vprsr.2023.100919
url http://hdl.handle.net/20.500.12123/15001
https://www.sciencedirect.com/science/article/pii/S2405939023000898
https://doi.org/10.1016/j.vprsr.2023.100919
identifier_str_mv 2405-9390
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Parasitology: Regional Studies and Reports 44 : 100919. (September 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.176822

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