Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies

Autores
Fiorani, Franco; Hecker, Yanina; Cirone, Karina Mariela; Armendano, Joaquín Ignacio; Gual, Ignacio; Campero, Lucía María; Wilkowsky, Silvina Elizabeth; Cobo, Eduardo Rubén; Corva, Pablo; Morrell, Eleonora Lidia; Canton, German Jose; Moore, Prando Dadin
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Delayed-type hypersensitivity (DTH) has been used in human and veterinary medicine as a skin testing for evaluating in vivocell-mediated immune responses (CMIR). Whereas CMIR is a key process to control intracellular pathogens, its value at identifying cattle exposed to the abortigenic intracellular coccidian parasite Neospora caninum is unknown. In this work, we have evaluated a DTH skin testing in cattle exposed to N. caninum and still seronegative. Female calves were experimentally sensitized by subcutaneous (SC) inoculation with live tachyzoites of N. caninum (NC-Argentina LP1) in sterile phosphate-buffered saline (PBS) (group A; n: 8) whereas other calveswere mock-sensitized with PBS (group B; n: 6). Two DTH skin tests were performed by intradermal inoculation with a soluble lysate of N. caninum tachyzoites (NC-Argentina LP1) in the neck region at 60d and 960 d after sensitization. Skinfold thickness at the intradermal inoculation site was measured at 0, 24, 48 h post each DTH skin test and skin biopsies taken for microscopic evaluation. Specific N. caninum antibodies kinetics was evaluated all throughthe experiment. We found that whereas N. caninum specific antibodies remained below the ELISA cut-off, a distinctive skinfold thickness increase was detected in sensitized animals (group A) at the DTH skin test site, showing induration, swelling and inflammatory infiltration. Mock sensitized animals (group B) showed no skinfold thickness growth and lacked specific antibody response. Thus, N. caninum DTH skin testing could be a useful diagnostic tool for the detection of CMIR during N. caninum infection in non-humoral responders
EEA Balcarce
Fil: Fiorani, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil. Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina
Fil.: Cirone, Karina Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA) Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Armendano, Joaquín Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Campero, Lucía María. Instituto Nacional de Tecnología Agropecuaria (INTA.) Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina. Universidad Nacional de La Plata. Facultad de Medicina Veterinaria; Argentina.
Fil: Wilkowsky, Silvia. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cobo, Eduardo Rubén. University of Calgary. Faculty of Veterinary Medicine; Canada
Fil: Corva, Pablo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Morrell, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Moore, Dadín Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fuente
Comparative Immunology, Microbiology and Infectious Diseases 72 : 101522 (2020)
Materia
Vaca
Neospora Caninun
Prueba Cutáneas
Hipersensibilidad Retardada
Respuesta Inmunológica
Cows
Skin Tests
Delayed Hypersensitivity
Immune Response
Heifers
Novilla
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7664
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7664
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spelling Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodiesFiorani, FrancoHecker, YaninaCirone, Karina MarielaArmendano, Joaquín IgnacioGual, IgnacioCampero, Lucía MaríaWilkowsky, Silvina ElizabethCobo, Eduardo RubénCorva, PabloMorrell, Eleonora LidiaCanton, German JoseMoore, Prando DadinVacaNeospora CaninunPrueba CutáneasHipersensibilidad RetardadaRespuesta InmunológicaCowsSkin TestsDelayed HypersensitivityImmune ResponseHeifersNovillaDelayed-type hypersensitivity (DTH) has been used in human and veterinary medicine as a skin testing for evaluating in vivocell-mediated immune responses (CMIR). Whereas CMIR is a key process to control intracellular pathogens, its value at identifying cattle exposed to the abortigenic intracellular coccidian parasite Neospora caninum is unknown. In this work, we have evaluated a DTH skin testing in cattle exposed to N. caninum and still seronegative. Female calves were experimentally sensitized by subcutaneous (SC) inoculation with live tachyzoites of N. caninum (NC-Argentina LP1) in sterile phosphate-buffered saline (PBS) (group A; n: 8) whereas other calveswere mock-sensitized with PBS (group B; n: 6). Two DTH skin tests were performed by intradermal inoculation with a soluble lysate of N. caninum tachyzoites (NC-Argentina LP1) in the neck region at 60d and 960 d after sensitization. Skinfold thickness at the intradermal inoculation site was measured at 0, 24, 48 h post each DTH skin test and skin biopsies taken for microscopic evaluation. Specific N. caninum antibodies kinetics was evaluated all throughthe experiment. We found that whereas N. caninum specific antibodies remained below the ELISA cut-off, a distinctive skinfold thickness increase was detected in sensitized animals (group A) at the DTH skin test site, showing induration, swelling and inflammatory infiltration. Mock sensitized animals (group B) showed no skinfold thickness growth and lacked specific antibody response. Thus, N. caninum DTH skin testing could be a useful diagnostic tool for the detection of CMIR during N. caninum infection in non-humoral respondersEEA BalcarceFil: Fiorani, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil. Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); ArgentinaFil.: Cirone, Karina Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA) Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Armendano, Joaquín Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; ArgentinaFil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Campero, Lucía María. Instituto Nacional de Tecnología Agropecuaria (INTA.) Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina. Universidad Nacional de La Plata. Facultad de Medicina Veterinaria; Argentina.Fil: Wilkowsky, Silvia. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cobo, Eduardo Rubén. University of Calgary. Faculty of Veterinary Medicine; CanadaFil: Corva, Pablo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Morrell, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Moore, Dadín Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaElsevier2020-08-04T11:17:06Z2020-08-04T11:17:06Z2020-07-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7664https://www.sciencedirect.com/science/article/abs/pii/S01479571203011190147-9571https://doi.org/10.1016/j.cimid.2020.101522Comparative Immunology, Microbiology and Infectious Diseases 72 : 101522 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:51Zoai:localhost:20.500.12123/7664instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:52.283INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies
title Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies
spellingShingle Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies
Fiorani, Franco
Vaca
Neospora Caninun
Prueba Cutáneas
Hipersensibilidad Retardada
Respuesta Inmunológica
Cows
Skin Tests
Delayed Hypersensitivity
Immune Response
Heifers
Novilla
title_short Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies
title_full Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies
title_fullStr Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies
title_full_unstemmed Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies
title_sort Delayed-type hypersensitivity skin test against Neospora caninum in heifers with undetectable specific antibodies
dc.creator.none.fl_str_mv Fiorani, Franco
Hecker, Yanina
Cirone, Karina Mariela
Armendano, Joaquín Ignacio
Gual, Ignacio
Campero, Lucía María
Wilkowsky, Silvina Elizabeth
Cobo, Eduardo Rubén
Corva, Pablo
Morrell, Eleonora Lidia
Canton, German Jose
Moore, Prando Dadin
author Fiorani, Franco
author_facet Fiorani, Franco
Hecker, Yanina
Cirone, Karina Mariela
Armendano, Joaquín Ignacio
Gual, Ignacio
Campero, Lucía María
Wilkowsky, Silvina Elizabeth
Cobo, Eduardo Rubén
Corva, Pablo
Morrell, Eleonora Lidia
Canton, German Jose
Moore, Prando Dadin
author_role author
author2 Hecker, Yanina
Cirone, Karina Mariela
Armendano, Joaquín Ignacio
Gual, Ignacio
Campero, Lucía María
Wilkowsky, Silvina Elizabeth
Cobo, Eduardo Rubén
Corva, Pablo
Morrell, Eleonora Lidia
Canton, German Jose
Moore, Prando Dadin
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Vaca
Neospora Caninun
Prueba Cutáneas
Hipersensibilidad Retardada
Respuesta Inmunológica
Cows
Skin Tests
Delayed Hypersensitivity
Immune Response
Heifers
Novilla
topic Vaca
Neospora Caninun
Prueba Cutáneas
Hipersensibilidad Retardada
Respuesta Inmunológica
Cows
Skin Tests
Delayed Hypersensitivity
Immune Response
Heifers
Novilla
dc.description.none.fl_txt_mv Delayed-type hypersensitivity (DTH) has been used in human and veterinary medicine as a skin testing for evaluating in vivocell-mediated immune responses (CMIR). Whereas CMIR is a key process to control intracellular pathogens, its value at identifying cattle exposed to the abortigenic intracellular coccidian parasite Neospora caninum is unknown. In this work, we have evaluated a DTH skin testing in cattle exposed to N. caninum and still seronegative. Female calves were experimentally sensitized by subcutaneous (SC) inoculation with live tachyzoites of N. caninum (NC-Argentina LP1) in sterile phosphate-buffered saline (PBS) (group A; n: 8) whereas other calveswere mock-sensitized with PBS (group B; n: 6). Two DTH skin tests were performed by intradermal inoculation with a soluble lysate of N. caninum tachyzoites (NC-Argentina LP1) in the neck region at 60d and 960 d after sensitization. Skinfold thickness at the intradermal inoculation site was measured at 0, 24, 48 h post each DTH skin test and skin biopsies taken for microscopic evaluation. Specific N. caninum antibodies kinetics was evaluated all throughthe experiment. We found that whereas N. caninum specific antibodies remained below the ELISA cut-off, a distinctive skinfold thickness increase was detected in sensitized animals (group A) at the DTH skin test site, showing induration, swelling and inflammatory infiltration. Mock sensitized animals (group B) showed no skinfold thickness growth and lacked specific antibody response. Thus, N. caninum DTH skin testing could be a useful diagnostic tool for the detection of CMIR during N. caninum infection in non-humoral responders
EEA Balcarce
Fil: Fiorani, Franco. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil. Hecker, Yanina Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina
Fil.: Cirone, Karina Mariela. Instituto Nacional de Tecnología Agropecuaria (INTA) Estación Experimental Agropecuaria Balcarce; Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Armendano, Joaquín Ignacio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias; Argentina
Fil: Gual, Ignacio. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Campero, Lucía María. Instituto Nacional de Tecnología Agropecuaria (INTA.) Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina. Universidad Nacional de La Plata. Facultad de Medicina Veterinaria; Argentina.
Fil: Wilkowsky, Silvia. Instituto Nacional de Tecnología Agropecuaria (INTA). UEDD IABIMO. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cobo, Eduardo Rubén. University of Calgary. Faculty of Veterinary Medicine; Canada
Fil: Corva, Pablo. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Morrell, Eleonora. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Cantón, Germán José. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Moore, Dadín Prando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS Balcarce); Argentina. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
description Delayed-type hypersensitivity (DTH) has been used in human and veterinary medicine as a skin testing for evaluating in vivocell-mediated immune responses (CMIR). Whereas CMIR is a key process to control intracellular pathogens, its value at identifying cattle exposed to the abortigenic intracellular coccidian parasite Neospora caninum is unknown. In this work, we have evaluated a DTH skin testing in cattle exposed to N. caninum and still seronegative. Female calves were experimentally sensitized by subcutaneous (SC) inoculation with live tachyzoites of N. caninum (NC-Argentina LP1) in sterile phosphate-buffered saline (PBS) (group A; n: 8) whereas other calveswere mock-sensitized with PBS (group B; n: 6). Two DTH skin tests were performed by intradermal inoculation with a soluble lysate of N. caninum tachyzoites (NC-Argentina LP1) in the neck region at 60d and 960 d after sensitization. Skinfold thickness at the intradermal inoculation site was measured at 0, 24, 48 h post each DTH skin test and skin biopsies taken for microscopic evaluation. Specific N. caninum antibodies kinetics was evaluated all throughthe experiment. We found that whereas N. caninum specific antibodies remained below the ELISA cut-off, a distinctive skinfold thickness increase was detected in sensitized animals (group A) at the DTH skin test site, showing induration, swelling and inflammatory infiltration. Mock sensitized animals (group B) showed no skinfold thickness growth and lacked specific antibody response. Thus, N. caninum DTH skin testing could be a useful diagnostic tool for the detection of CMIR during N. caninum infection in non-humoral responders
publishDate 2020
dc.date.none.fl_str_mv 2020-08-04T11:17:06Z
2020-08-04T11:17:06Z
2020-07-19
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7664
https://www.sciencedirect.com/science/article/abs/pii/S0147957120301119
0147-9571
https://doi.org/10.1016/j.cimid.2020.101522
url http://hdl.handle.net/20.500.12123/7664
https://www.sciencedirect.com/science/article/abs/pii/S0147957120301119
https://doi.org/10.1016/j.cimid.2020.101522
identifier_str_mv 0147-9571
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Comparative Immunology, Microbiology and Infectious Diseases 72 : 101522 (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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