Leaf structure and ultrastructure changes induced by heat stress and drought during seed filling in field-grown soybean and their relationship with grain yield

Autores
Carrera, Constanza Soledad; Solis, Stella M.; Ferricci, María S.; Vega, Claudia Rosa Cecilia; Galati, Beatriz G.; Ergo, Veronica Vanesa; Andrade, Fernando Hector; Lascano, Hernan Ramiro
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Studies focusing on terminal drought combined with heat impacts on plants of agronomic value remain scarce, and even less under field conditions. The objective of this study was to investigate leaf structural and ultrastructural changes induced by heat stress (HS) and drought stress (DS) during seed filling and their relationship with physiological variables and yield determination. Two soybean cultivars were grown in field conditions. During seed filling four treatments were applied, including a control (without manipulation, at ambient temperature and field capacity), HS (episodes exceeding 32°C for 6 h d-1) during 21-d, DS (20% of field capacity soil water content) during 35-d, and HS×DS. Drought principally reduced leaf area, whereas heat decreased leaf thickness, possible as acclimation strategies, but also irreversible reducing CO2 assimilation sites. Both stresses damaged the outer and inner membranes of chloroplasts, causing swollen chloroplasts and accumulation of plastoglobules, loss of chlorophyll content, and negatively affecting chlorophyll fluorescence. Thus, the performance and integrity of the photosynthetic machinery were reduced. Through a morpho-functional perspective and a holistic multiscale approach, our results provide evidence of photosynthesis impairment and yield drops under stressful conditions which were associated with structural and ultrastructural (particularly at the level of chloroplasts) modifications of leaves.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Carrera, Constanza Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Carrera, Constanza Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Solis, Stella M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste (IBONE); Argentina
Fil: Solis, Stella M. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Cátedra de Morfología Vegetal; Argentina
Fil: Ferricci, María S. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferricci, María S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste (IBONE); Argentina
Fil: Vega, Claudia Rosa Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Galati, Beatriz G. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Botánico General; Argentina
Fil: Ergo, Verónica Vanesa. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Ergo, Verónica Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Andrade, Fernando Hector. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Ecofisiología de cultivos; Argentina
Fil: Andrade, Fernando Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal. Argentina
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA). Argentina
Fuente
Anais da Academia Brasileira de Ciências 93 (4) : e20191388 (2021)
Materia
Déficit Hídrico
Sequía
Estrés Térmico
Soybeans
Drought
Heat Stress
Water Shortage
Soja
Episodes of High Temperatures
Late Reproductive Development
Leaf Morpho-Anatomy
Leaf Photosynthetic Performance
Seed Number and Weight
Water Deficit
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10001
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10001
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Leaf structure and ultrastructure changes induced by heat stress and drought during seed filling in field-grown soybean and their relationship with grain yieldCarrera, Constanza SoledadSolis, Stella M.Ferricci, María S.Vega, Claudia Rosa CeciliaGalati, Beatriz G.Ergo, Veronica VanesaAndrade, Fernando HectorLascano, Hernan RamiroDéficit HídricoSequíaEstrés TérmicoSoybeansDroughtHeat StressWater ShortageSojaEpisodes of High TemperaturesLate Reproductive DevelopmentLeaf Morpho-AnatomyLeaf Photosynthetic PerformanceSeed Number and WeightWater DeficitStudies focusing on terminal drought combined with heat impacts on plants of agronomic value remain scarce, and even less under field conditions. The objective of this study was to investigate leaf structural and ultrastructural changes induced by heat stress (HS) and drought stress (DS) during seed filling and their relationship with physiological variables and yield determination. Two soybean cultivars were grown in field conditions. During seed filling four treatments were applied, including a control (without manipulation, at ambient temperature and field capacity), HS (episodes exceeding 32°C for 6 h d-1) during 21-d, DS (20% of field capacity soil water content) during 35-d, and HS×DS. Drought principally reduced leaf area, whereas heat decreased leaf thickness, possible as acclimation strategies, but also irreversible reducing CO2 assimilation sites. Both stresses damaged the outer and inner membranes of chloroplasts, causing swollen chloroplasts and accumulation of plastoglobules, loss of chlorophyll content, and negatively affecting chlorophyll fluorescence. Thus, the performance and integrity of the photosynthetic machinery were reduced. Through a morpho-functional perspective and a holistic multiscale approach, our results provide evidence of photosynthesis impairment and yield drops under stressful conditions which were associated with structural and ultrastructural (particularly at the level of chloroplasts) modifications of leaves.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Carrera, Constanza Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; ArgentinaFil: Carrera, Constanza Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Solis, Stella M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste (IBONE); ArgentinaFil: Solis, Stella M. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Cátedra de Morfología Vegetal; ArgentinaFil: Ferricci, María S. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferricci, María S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste (IBONE); ArgentinaFil: Vega, Claudia Rosa Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; ArgentinaFil: Galati, Beatriz G. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Botánico General; ArgentinaFil: Ergo, Verónica Vanesa. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Ergo, Verónica Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); ArgentinaFil: Andrade, Fernando Hector. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Ecofisiología de cultivos; ArgentinaFil: Andrade, Fernando Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal. ArgentinaFil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA). ArgentinaAcademia Brasileira de Ciências2021-08-11T11:29:08Z2021-08-11T11:29:08Z2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10001https://www.scielo.br/j/aabc/a/Ybf8nVnzvYGF5ptzdsWPdqx/abstract/?lang=en#0001-37651678-2690 (online)https://doi.org/10.1590/0001-3765202120191388Anais da Academia Brasileira de Ciências 93 (4) : e20191388 (2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/PNAGUA-1133032/AR./Herramientas para la mitigacion de la incidencia del estres abiótico en cultivos.info:eu-repograntAgreement/INTA/PNCYO-1127042/AR./Bases ecofisiológicas para el mejoramiento genético y la calidad diferenciada de cereales y oleaginosas.info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:18Zoai:localhost:20.500.12123/10001instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:18.644INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Leaf structure and ultrastructure changes induced by heat stress and drought during seed filling in field-grown soybean and their relationship with grain yield
title Leaf structure and ultrastructure changes induced by heat stress and drought during seed filling in field-grown soybean and their relationship with grain yield
spellingShingle Leaf structure and ultrastructure changes induced by heat stress and drought during seed filling in field-grown soybean and their relationship with grain yield
Carrera, Constanza Soledad
Déficit Hídrico
Sequía
Estrés Térmico
Soybeans
Drought
Heat Stress
Water Shortage
Soja
Episodes of High Temperatures
Late Reproductive Development
Leaf Morpho-Anatomy
Leaf Photosynthetic Performance
Seed Number and Weight
Water Deficit
title_short Leaf structure and ultrastructure changes induced by heat stress and drought during seed filling in field-grown soybean and their relationship with grain yield
title_full Leaf structure and ultrastructure changes induced by heat stress and drought during seed filling in field-grown soybean and their relationship with grain yield
title_fullStr Leaf structure and ultrastructure changes induced by heat stress and drought during seed filling in field-grown soybean and their relationship with grain yield
title_full_unstemmed Leaf structure and ultrastructure changes induced by heat stress and drought during seed filling in field-grown soybean and their relationship with grain yield
title_sort Leaf structure and ultrastructure changes induced by heat stress and drought during seed filling in field-grown soybean and their relationship with grain yield
dc.creator.none.fl_str_mv Carrera, Constanza Soledad
Solis, Stella M.
Ferricci, María S.
Vega, Claudia Rosa Cecilia
Galati, Beatriz G.
Ergo, Veronica Vanesa
Andrade, Fernando Hector
Lascano, Hernan Ramiro
author Carrera, Constanza Soledad
author_facet Carrera, Constanza Soledad
Solis, Stella M.
Ferricci, María S.
Vega, Claudia Rosa Cecilia
Galati, Beatriz G.
Ergo, Veronica Vanesa
Andrade, Fernando Hector
Lascano, Hernan Ramiro
author_role author
author2 Solis, Stella M.
Ferricci, María S.
Vega, Claudia Rosa Cecilia
Galati, Beatriz G.
Ergo, Veronica Vanesa
Andrade, Fernando Hector
Lascano, Hernan Ramiro
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Déficit Hídrico
Sequía
Estrés Térmico
Soybeans
Drought
Heat Stress
Water Shortage
Soja
Episodes of High Temperatures
Late Reproductive Development
Leaf Morpho-Anatomy
Leaf Photosynthetic Performance
Seed Number and Weight
Water Deficit
topic Déficit Hídrico
Sequía
Estrés Térmico
Soybeans
Drought
Heat Stress
Water Shortage
Soja
Episodes of High Temperatures
Late Reproductive Development
Leaf Morpho-Anatomy
Leaf Photosynthetic Performance
Seed Number and Weight
Water Deficit
dc.description.none.fl_txt_mv Studies focusing on terminal drought combined with heat impacts on plants of agronomic value remain scarce, and even less under field conditions. The objective of this study was to investigate leaf structural and ultrastructural changes induced by heat stress (HS) and drought stress (DS) during seed filling and their relationship with physiological variables and yield determination. Two soybean cultivars were grown in field conditions. During seed filling four treatments were applied, including a control (without manipulation, at ambient temperature and field capacity), HS (episodes exceeding 32°C for 6 h d-1) during 21-d, DS (20% of field capacity soil water content) during 35-d, and HS×DS. Drought principally reduced leaf area, whereas heat decreased leaf thickness, possible as acclimation strategies, but also irreversible reducing CO2 assimilation sites. Both stresses damaged the outer and inner membranes of chloroplasts, causing swollen chloroplasts and accumulation of plastoglobules, loss of chlorophyll content, and negatively affecting chlorophyll fluorescence. Thus, the performance and integrity of the photosynthetic machinery were reduced. Through a morpho-functional perspective and a holistic multiscale approach, our results provide evidence of photosynthesis impairment and yield drops under stressful conditions which were associated with structural and ultrastructural (particularly at the level of chloroplasts) modifications of leaves.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Carrera, Constanza Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina
Fil: Carrera, Constanza Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Solis, Stella M. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste (IBONE); Argentina
Fil: Solis, Stella M. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Cátedra de Morfología Vegetal; Argentina
Fil: Ferricci, María S. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferricci, María S. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Botánica del Nordeste (IBONE); Argentina
Fil: Vega, Claudia Rosa Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Manfredi; Argentina
Fil: Galati, Beatriz G. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Botánico General; Argentina
Fil: Ergo, Verónica Vanesa. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Ergo, Verónica Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA); Argentina
Fil: Andrade, Fernando Hector. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Ecofisiología de cultivos; Argentina
Fil: Andrade, Fernando Hector. Consejo Nacional de Investigaciones Científicas y Técnicas. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Lascano, Hernán Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal. Argentina
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Estudios Agropecuarios (UDEA). Argentina
description Studies focusing on terminal drought combined with heat impacts on plants of agronomic value remain scarce, and even less under field conditions. The objective of this study was to investigate leaf structural and ultrastructural changes induced by heat stress (HS) and drought stress (DS) during seed filling and their relationship with physiological variables and yield determination. Two soybean cultivars were grown in field conditions. During seed filling four treatments were applied, including a control (without manipulation, at ambient temperature and field capacity), HS (episodes exceeding 32°C for 6 h d-1) during 21-d, DS (20% of field capacity soil water content) during 35-d, and HS×DS. Drought principally reduced leaf area, whereas heat decreased leaf thickness, possible as acclimation strategies, but also irreversible reducing CO2 assimilation sites. Both stresses damaged the outer and inner membranes of chloroplasts, causing swollen chloroplasts and accumulation of plastoglobules, loss of chlorophyll content, and negatively affecting chlorophyll fluorescence. Thus, the performance and integrity of the photosynthetic machinery were reduced. Through a morpho-functional perspective and a holistic multiscale approach, our results provide evidence of photosynthesis impairment and yield drops under stressful conditions which were associated with structural and ultrastructural (particularly at the level of chloroplasts) modifications of leaves.
publishDate 2021
dc.date.none.fl_str_mv 2021-08-11T11:29:08Z
2021-08-11T11:29:08Z
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10001
https://www.scielo.br/j/aabc/a/Ybf8nVnzvYGF5ptzdsWPdqx/abstract/?lang=en#
0001-3765
1678-2690 (online)
https://doi.org/10.1590/0001-3765202120191388
url http://hdl.handle.net/20.500.12123/10001
https://www.scielo.br/j/aabc/a/Ybf8nVnzvYGF5ptzdsWPdqx/abstract/?lang=en#
https://doi.org/10.1590/0001-3765202120191388
identifier_str_mv 0001-3765
1678-2690 (online)
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNAGUA-1133032/AR./Herramientas para la mitigacion de la incidencia del estres abiótico en cultivos.
info:eu-repograntAgreement/INTA/PNCYO-1127042/AR./Bases ecofisiológicas para el mejoramiento genético y la calidad diferenciada de cereales y oleaginosas.
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv Anais da Academia Brasileira de Ciências 93 (4) : e20191388 (2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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