Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice

Autores
Ruiz, Vanesa; Baztarrica, Josefina; Rybicki, Edward P.; Meyers, Ann E.; Wigdorovitz, Andres
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.
Instituto de Virología
Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Baztarrica, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rybicki, Edward P. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica. University of Cape Town. Faculty of Health Sciences. Institute of Infectious Disease and Molecular Medicine; Sudáfrica
Fil: Meyers, Ann E. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Biotechnology reports 20 : e00283. (December 2018)
Materia
Extractos de Hoja
Virus Fiebre Aftosa
Inmunogenética
Ratón
Leaf Extracts
Aphthovirus
immunogenetics
Mice
Nicotiana Benthamiana
Foot and Mouth Disease Virus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4443
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4443
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repository_id_str l
network_name_str INTA Digital (INTA)
spelling Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in miceRuiz, VanesaBaztarrica, JosefinaRybicki, Edward P.Meyers, Ann E.Wigdorovitz, AndresExtractos de HojaVirus Fiebre AftosaInmunogenéticaRatónLeaf ExtractsAphthovirusimmunogeneticsMiceNicotiana BenthamianaFoot and Mouth Disease VirusFoot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.Instituto de VirologíaFil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaFil: Baztarrica, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Rybicki, Edward P. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica. University of Cape Town. Faculty of Health Sciences. Institute of Infectious Disease and Molecular Medicine; SudáfricaFil: Meyers, Ann E. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; SudáfricaFil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2019-02-14T15:10:30Z2019-02-14T15:10:30Z2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4443https://www.sciencedirect.com/science/article/pii/S2215017X1830050X?via%3Dihub2215-017Xhttps://doi.org/10.1016/j.btre.2018.e00283Biotechnology reports 20 : e00283. (December 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-11-06T09:39:54Zoai:localhost:20.500.12123/4443instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-06 09:39:54.925INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
title Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
spellingShingle Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
Ruiz, Vanesa
Extractos de Hoja
Virus Fiebre Aftosa
Inmunogenética
Ratón
Leaf Extracts
Aphthovirus
immunogenetics
Mice
Nicotiana Benthamiana
Foot and Mouth Disease Virus
title_short Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
title_full Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
title_fullStr Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
title_full_unstemmed Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
title_sort Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
dc.creator.none.fl_str_mv Ruiz, Vanesa
Baztarrica, Josefina
Rybicki, Edward P.
Meyers, Ann E.
Wigdorovitz, Andres
author Ruiz, Vanesa
author_facet Ruiz, Vanesa
Baztarrica, Josefina
Rybicki, Edward P.
Meyers, Ann E.
Wigdorovitz, Andres
author_role author
author2 Baztarrica, Josefina
Rybicki, Edward P.
Meyers, Ann E.
Wigdorovitz, Andres
author2_role author
author
author
author
dc.subject.none.fl_str_mv Extractos de Hoja
Virus Fiebre Aftosa
Inmunogenética
Ratón
Leaf Extracts
Aphthovirus
immunogenetics
Mice
Nicotiana Benthamiana
Foot and Mouth Disease Virus
topic Extractos de Hoja
Virus Fiebre Aftosa
Inmunogenética
Ratón
Leaf Extracts
Aphthovirus
immunogenetics
Mice
Nicotiana Benthamiana
Foot and Mouth Disease Virus
dc.description.none.fl_txt_mv Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.
Instituto de Virología
Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Baztarrica, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rybicki, Edward P. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica. University of Cape Town. Faculty of Health Sciences. Institute of Infectious Disease and Molecular Medicine; Sudáfrica
Fil: Meyers, Ann E. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.
publishDate 2018
dc.date.none.fl_str_mv 2018-12
2019-02-14T15:10:30Z
2019-02-14T15:10:30Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4443
https://www.sciencedirect.com/science/article/pii/S2215017X1830050X?via%3Dihub
2215-017X
https://doi.org/10.1016/j.btre.2018.e00283
url http://hdl.handle.net/20.500.12123/4443
https://www.sciencedirect.com/science/article/pii/S2215017X1830050X?via%3Dihub
https://doi.org/10.1016/j.btre.2018.e00283
identifier_str_mv 2215-017X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Biotechnology reports 20 : e00283. (December 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.976206

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