Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice
- Autores
- Ruiz, Vanesa; Baztarrica, Josefina; Rybicki, Edward P.; Meyers, Ann E.; Wigdorovitz, Andres
- Año de publicación
- 2018
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.
Instituto de Virología
Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina
Fil: Baztarrica, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rybicki, Edward P. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica. University of Cape Town. Faculty of Health Sciences. Institute of Infectious Disease and Molecular Medicine; Sudáfrica
Fil: Meyers, Ann E. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Biotechnology reports 20 : e00283. (December 2018)
- Materia
-
Extractos de Hoja
Virus Fiebre Aftosa
Inmunogenética
Ratón
Leaf Extracts
Aphthovirus
immunogenetics
Mice
Nicotiana Benthamiana
Foot and Mouth Disease Virus - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/4443
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Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in miceRuiz, VanesaBaztarrica, JosefinaRybicki, Edward P.Meyers, Ann E.Wigdorovitz, AndresExtractos de HojaVirus Fiebre AftosaInmunogenéticaRatónLeaf ExtractsAphthovirusimmunogeneticsMiceNicotiana BenthamianaFoot and Mouth Disease VirusFoot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV.Instituto de VirologíaFil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; ArgentinaFil: Baztarrica, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Rybicki, Edward P. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica. University of Cape Town. Faculty of Health Sciences. Institute of Infectious Disease and Molecular Medicine; SudáfricaFil: Meyers, Ann E. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; SudáfricaFil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2019-02-14T15:10:30Z2019-02-14T15:10:30Z2018-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4443https://www.sciencedirect.com/science/article/pii/S2215017X1830050X?via%3Dihub2215-017Xhttps://doi.org/10.1016/j.btre.2018.e00283Biotechnology reports 20 : e00283. (December 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:34Zoai:localhost:20.500.12123/4443instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:34.978INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice |
title |
Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice |
spellingShingle |
Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice Ruiz, Vanesa Extractos de Hoja Virus Fiebre Aftosa Inmunogenética Ratón Leaf Extracts Aphthovirus immunogenetics Mice Nicotiana Benthamiana Foot and Mouth Disease Virus |
title_short |
Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice |
title_full |
Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice |
title_fullStr |
Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice |
title_full_unstemmed |
Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice |
title_sort |
Minimally processed crude leaf extracts of Nicotiana benthamiana containing recombinant foot and mouth disease virus-like particles are immunogenic in mice |
dc.creator.none.fl_str_mv |
Ruiz, Vanesa Baztarrica, Josefina Rybicki, Edward P. Meyers, Ann E. Wigdorovitz, Andres |
author |
Ruiz, Vanesa |
author_facet |
Ruiz, Vanesa Baztarrica, Josefina Rybicki, Edward P. Meyers, Ann E. Wigdorovitz, Andres |
author_role |
author |
author2 |
Baztarrica, Josefina Rybicki, Edward P. Meyers, Ann E. Wigdorovitz, Andres |
author2_role |
author author author author |
dc.subject.none.fl_str_mv |
Extractos de Hoja Virus Fiebre Aftosa Inmunogenética Ratón Leaf Extracts Aphthovirus immunogenetics Mice Nicotiana Benthamiana Foot and Mouth Disease Virus |
topic |
Extractos de Hoja Virus Fiebre Aftosa Inmunogenética Ratón Leaf Extracts Aphthovirus immunogenetics Mice Nicotiana Benthamiana Foot and Mouth Disease Virus |
dc.description.none.fl_txt_mv |
Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV. Instituto de Virología Fil: Ruiz, Vanesa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Tecnológicas; Argentina Fil: Baztarrica, Josefina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Rybicki, Edward P. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica. University of Cape Town. Faculty of Health Sciences. Institute of Infectious Disease and Molecular Medicine; Sudáfrica Fil: Meyers, Ann E. University of Cape Town. Department of Molecular and cell Biology. Biopharming Research Unit; Sudáfrica Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Foot-and-mouth disease (FMD) remains one of the most feared viral diseases affecting cloven-hoofed animals, and results in severe economic losses. Currently available vaccines are based on inactivated FMD virus (FMDV). The use of recombinant FMDV-like particles (VLPs) as subunit vaccines has gained importance because of their immunogenic properties and safety. We evaluated the production of FMD VLPs, via Agrobacterium-mediated transient expression, and the immunogenicity of these structures in mice. Leaves were infiltrated with pEAQ-HT and pRIC 3.0 vectors encoding the capsid precursor P1-2A and the protease 3C. The recombinant protein yield was 3–4 mg/kg of fresh leaf tissue. Both groups of mice immunized with purified VLPs and mice immunized with the crude leaf extract elicited a specific humoral response with similar antibody titers. Thus, minimally processed plant material containing transiently expressed FMD VLPs could be a scalable and cost-effective technology for the production of a recombinant subunit vaccine against FMDV. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12 2019-02-14T15:10:30Z 2019-02-14T15:10:30Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/4443 https://www.sciencedirect.com/science/article/pii/S2215017X1830050X?via%3Dihub 2215-017X https://doi.org/10.1016/j.btre.2018.e00283 |
url |
http://hdl.handle.net/20.500.12123/4443 https://www.sciencedirect.com/science/article/pii/S2215017X1830050X?via%3Dihub https://doi.org/10.1016/j.btre.2018.e00283 |
identifier_str_mv |
2215-017X |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
Biotechnology reports 20 : e00283. (December 2018) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
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1844619130888519680 |
score |
12.559606 |