Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata

Autores: Devescovi, Francisco; Fernández, Patricia; Bachmann, Guillermo Enrique; Nussenbaum, Ana Laura; Segura, Diego Fernando
Año de publicación: 2024
Idioma: inglés
Tipo de recurso: artículo
Estado: versión publicada
Descripción: Fruit fly pests (Diptera: Tephritidae) are a serious problem for fruit production and for local and international trade. Biological control is increasingly included as a pest control tool within integrated pest management (IPM) programmes, seeking to reduce pesticides and improve fruit quality. Ceratitis capitata Wiedemann, the Mediterranean fruit fly (medfly), is probably the most damaging fruit fly pest, with a global distribution and more than 200 host species. The solitary larval endoparasitoid Diachasmimorpha longicaudata Ashmead (Hymenoptera: Braconidae) is a biocontrol agent widely used against Tephritidae fruit fly pests. Previous studies showed that female wasps locate host larvae using visual, mechanical, and chemical cues. Here, we investigated the chemical basis of female parasitoid attraction to cues that guide D. longicaudata to the host, and thus unveil volatile organic compounds that might be used in IPM programmes. Female orientation to chemical cues was tested in a Y-tube olfactometer, where attraction to C. capitata-infested oranges, oranges with residues of larval activity, oranges infected with a green mould, and overripe oranges was confirmed. Volatiles from all these types of fruit were collected and used in gas chromatography-electroantennographic detection (GC-EAD) and GC-mass spectrometry (MS) analyses. These studies allowed us to identify six candidate compounds that were present in all treated oranges but not in the control fruit (ripe and uninfested oranges): D-limonene, acetophenone, linalool, nonanal, decanal, and eugenol. Electroantennography (EAG) showed that acetophenone, nonanal, and decanal triggered dose-dependent responses, suggesting a relevant role in the process of host finding. Although responses to D-limonene, linalool, and eugenol were independent of the dose, they could be involved in host location in areas with high probability of host presence. The fact that these six compounds are shared by the four behaviourally attractive sources opens new possibilities for the development of novel tools to improve biocontrol programmes.
Instituto de Genética
Fil: Devescovi, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Devescovi, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Devescovi, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fernández, Patricia. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Química de Biomoléculas; Argentina
Fil: Fernández, Patricia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica. Centro de Investigaciones en Hidratos de Carbono; Argentina
Fil: Fernández, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bachmann, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Bachmann, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Bachmann, Guillermo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Nussenbaum, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nussenbaum, Ana Laura. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Segura, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Segura, Diego Fernando. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina
Fuente: Entomologia Experimentalis et Applicata : 1–11 (First published: 06 February 2024)
Materia: Attractants
Biological Control
Braconidae
Chemical Ecology
Tephritidae
Hymenoptera
Volatile Compounds
Hosts
Atrayentes
Control Biológico
Ceratitis capitata
Ecología Química
Compuesto Volátil
Huéspedes
Attractants
Carnada
Nivel de accesibilidad: acceso restringido
Condiciones de uso: http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Institución: Instituto Nacional de Tecnología Agropecuaria
OAI Identificador: oai:localhost:20.500.12123/17329

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spelling	Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudataDevescovi, FranciscoFernández, PatriciaBachmann, Guillermo EnriqueNussenbaum, Ana LauraSegura, Diego FernandoAttractantsBiological ControlBraconidaeChemical EcologyTephritidaeHymenopteraVolatile CompoundsHostsAtrayentesControl BiológicoCeratitis capitataEcología QuímicaCompuesto VolátilHuéspedesAttractantsCarnadaFruit fly pests (Diptera: Tephritidae) are a serious problem for fruit production and for local and international trade. Biological control is increasingly included as a pest control tool within integrated pest management (IPM) programmes, seeking to reduce pesticides and improve fruit quality. Ceratitis capitata Wiedemann, the Mediterranean fruit fly (medfly), is probably the most damaging fruit fly pest, with a global distribution and more than 200 host species. The solitary larval endoparasitoid Diachasmimorpha longicaudata Ashmead (Hymenoptera: Braconidae) is a biocontrol agent widely used against Tephritidae fruit fly pests. Previous studies showed that female wasps locate host larvae using visual, mechanical, and chemical cues. Here, we investigated the chemical basis of female parasitoid attraction to cues that guide D. longicaudata to the host, and thus unveil volatile organic compounds that might be used in IPM programmes. Female orientation to chemical cues was tested in a Y-tube olfactometer, where attraction to C. capitata-infested oranges, oranges with residues of larval activity, oranges infected with a green mould, and overripe oranges was confirmed. Volatiles from all these types of fruit were collected and used in gas chromatography-electroantennographic detection (GC-EAD) and GC-mass spectrometry (MS) analyses. These studies allowed us to identify six candidate compounds that were present in all treated oranges but not in the control fruit (ripe and uninfested oranges): D-limonene, acetophenone, linalool, nonanal, decanal, and eugenol. Electroantennography (EAG) showed that acetophenone, nonanal, and decanal triggered dose-dependent responses, suggesting a relevant role in the process of host finding. Although responses to D-limonene, linalool, and eugenol were independent of the dose, they could be involved in host location in areas with high probability of host presence. The fact that these six compounds are shared by the four behaviourally attractive sources opens new possibilities for the development of novel tools to improve biocontrol programmes.Instituto de GenéticaFil: Devescovi, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Devescovi, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Devescovi, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fernández, Patricia. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Química de Biomoléculas; ArgentinaFil: Fernández, Patricia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica. Centro de Investigaciones en Hidratos de Carbono; ArgentinaFil: Fernández, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bachmann, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Bachmann, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Bachmann, Guillermo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Nussenbaum, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nussenbaum, Ana Laura. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; ArgentinaFil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Segura, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Segura, Diego Fernando. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; ArgentinaWiley2024-04-08T13:43:06Z2024-04-08T13:43:06Z2024-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/17329https://onlinelibrary.wiley.com/doi/10.1111/eea.134171570-7458https://doi.org/10.1111/eea.13417Entomologia Experimentalis et Applicata : 1–11 (First published: 06 February 2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E4-I079-001, Genética, genómica y ecología de insectos de importancia agronómica como insumo para el desarrollo de estrategias sustentables de control plagasinfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-11T10:25:02Zoai:localhost:20.500.12123/17329instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:25:02.776INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv	Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata
title	Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata
spellingShingle	Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata Devescovi, Francisco Attractants Biological Control Braconidae Chemical Ecology Tephritidae Hymenoptera Volatile Compounds Hosts Atrayentes Control Biológico Ceratitis capitata Ecología Química Compuesto Volátil Huéspedes Attractants Carnada
title_short	Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata
title_full	Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata
title_fullStr	Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata
title_full_unstemmed	Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata
title_sort	Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata
dc.creator.none.fl_str_mv	Devescovi, Francisco Fernández, Patricia Bachmann, Guillermo Enrique Nussenbaum, Ana Laura Segura, Diego Fernando
author	Devescovi, Francisco
author_facet	Devescovi, Francisco Fernández, Patricia Bachmann, Guillermo Enrique Nussenbaum, Ana Laura Segura, Diego Fernando
author_role	author
author2	Fernández, Patricia Bachmann, Guillermo Enrique Nussenbaum, Ana Laura Segura, Diego Fernando
author2_role	author author author author
dc.subject.none.fl_str_mv	Attractants Biological Control Braconidae Chemical Ecology Tephritidae Hymenoptera Volatile Compounds Hosts Atrayentes Control Biológico Ceratitis capitata Ecología Química Compuesto Volátil Huéspedes Attractants Carnada
topic	Attractants Biological Control Braconidae Chemical Ecology Tephritidae Hymenoptera Volatile Compounds Hosts Atrayentes Control Biológico Ceratitis capitata Ecología Química Compuesto Volátil Huéspedes Attractants Carnada
dc.description.none.fl_txt_mv	Fruit fly pests (Diptera: Tephritidae) are a serious problem for fruit production and for local and international trade. Biological control is increasingly included as a pest control tool within integrated pest management (IPM) programmes, seeking to reduce pesticides and improve fruit quality. Ceratitis capitata Wiedemann, the Mediterranean fruit fly (medfly), is probably the most damaging fruit fly pest, with a global distribution and more than 200 host species. The solitary larval endoparasitoid Diachasmimorpha longicaudata Ashmead (Hymenoptera: Braconidae) is a biocontrol agent widely used against Tephritidae fruit fly pests. Previous studies showed that female wasps locate host larvae using visual, mechanical, and chemical cues. Here, we investigated the chemical basis of female parasitoid attraction to cues that guide D. longicaudata to the host, and thus unveil volatile organic compounds that might be used in IPM programmes. Female orientation to chemical cues was tested in a Y-tube olfactometer, where attraction to C. capitata-infested oranges, oranges with residues of larval activity, oranges infected with a green mould, and overripe oranges was confirmed. Volatiles from all these types of fruit were collected and used in gas chromatography-electroantennographic detection (GC-EAD) and GC-mass spectrometry (MS) analyses. These studies allowed us to identify six candidate compounds that were present in all treated oranges but not in the control fruit (ripe and uninfested oranges): D-limonene, acetophenone, linalool, nonanal, decanal, and eugenol. Electroantennography (EAG) showed that acetophenone, nonanal, and decanal triggered dose-dependent responses, suggesting a relevant role in the process of host finding. Although responses to D-limonene, linalool, and eugenol were independent of the dose, they could be involved in host location in areas with high probability of host presence. The fact that these six compounds are shared by the four behaviourally attractive sources opens new possibilities for the development of novel tools to improve biocontrol programmes. Instituto de Genética Fil: Devescovi, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Devescovi, Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Devescovi, Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Fernández, Patricia. Universidad de Buenos Aires. Facultad de Agronomía. Cátedra de Química de Biomoléculas; Argentina Fil: Fernández, Patricia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Departamento de Química Orgánica. Centro de Investigaciones en Hidratos de Carbono; Argentina Fil: Fernández, Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Bachmann, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Bachmann, Guillermo Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Bachmann, Guillermo Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Nussenbaum, Ana Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Nussenbaum, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nussenbaum, Ana Laura. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina Fil: Segura, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Segura, Diego Fernando. Universidad del Salvador. Facultad de Ciencias Agrarias y Veterinarias; Argentina
description	Fruit fly pests (Diptera: Tephritidae) are a serious problem for fruit production and for local and international trade. Biological control is increasingly included as a pest control tool within integrated pest management (IPM) programmes, seeking to reduce pesticides and improve fruit quality. Ceratitis capitata Wiedemann, the Mediterranean fruit fly (medfly), is probably the most damaging fruit fly pest, with a global distribution and more than 200 host species. The solitary larval endoparasitoid Diachasmimorpha longicaudata Ashmead (Hymenoptera: Braconidae) is a biocontrol agent widely used against Tephritidae fruit fly pests. Previous studies showed that female wasps locate host larvae using visual, mechanical, and chemical cues. Here, we investigated the chemical basis of female parasitoid attraction to cues that guide D. longicaudata to the host, and thus unveil volatile organic compounds that might be used in IPM programmes. Female orientation to chemical cues was tested in a Y-tube olfactometer, where attraction to C. capitata-infested oranges, oranges with residues of larval activity, oranges infected with a green mould, and overripe oranges was confirmed. Volatiles from all these types of fruit were collected and used in gas chromatography-electroantennographic detection (GC-EAD) and GC-mass spectrometry (MS) analyses. These studies allowed us to identify six candidate compounds that were present in all treated oranges but not in the control fruit (ripe and uninfested oranges): D-limonene, acetophenone, linalool, nonanal, decanal, and eugenol. Electroantennography (EAG) showed that acetophenone, nonanal, and decanal triggered dose-dependent responses, suggesting a relevant role in the process of host finding. Although responses to D-limonene, linalool, and eugenol were independent of the dose, they could be involved in host location in areas with high probability of host presence. The fact that these six compounds are shared by the four behaviourally attractive sources opens new possibilities for the development of novel tools to improve biocontrol programmes.
publishDate	2024
dc.date.none.fl_str_mv	2024-04-08T13:43:06Z 2024-04-08T13:43:06Z 2024-02
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dc.identifier.none.fl_str_mv	http://hdl.handle.net/20.500.12123/17329 https://onlinelibrary.wiley.com/doi/10.1111/eea.13417 1570-7458 https://doi.org/10.1111/eea.13417
url	http://hdl.handle.net/20.500.12123/17329 https://onlinelibrary.wiley.com/doi/10.1111/eea.13417 https://doi.org/10.1111/eea.13417
identifier_str_mv	1570-7458
dc.language.none.fl_str_mv	eng
language	eng
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dc.rights.none.fl_str_mv	info:eu-repo/semantics/restrictedAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
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dc.publisher.none.fl_str_mv	Wiley
publisher.none.fl_str_mv	Wiley
dc.source.none.fl_str_mv	Entomologia Experimentalis et Applicata : 1–11 (First published: 06 February 2024) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria
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Direct and indirect host-related volatile compounds attract a fruit fly parasitoid, Diachasmimorpha longicaudata

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