Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein

Autores
Distefano, Ana Julia; Maldonado, Sara Beatriz; Hopp, Horacio Esteban; Del Vas, Mariana
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications, we positively identified five structural proteins of approximately 170, 140, 130, 66, and 62 kDa. The protein encoded by MRCV S3 genomic segment was expressed as a fusion protein in Escherichia coli, purified and used for rabbit immunization. The resulting antiserum reacted with the 140 kDa structural protein and with incomplete virus particles in immunoelectron microscopy assays, suggesting that MRCV S3 codes for the major core capsid protein.
Instituto de Biotecnología
Fil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Distefano, Ana Julia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Maldonado, Sara Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Virus Genes 38 (3) : 455-460 (Junio 2009)
Materia
Fijivirus
Maíz
Proteínas Estructurales
Maize
Structural Proteins
Mal de Río Cuarto Virus
Capsid
Virus del Mal de Río Cuarto
Cápside
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15366
Acceder
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oai_identifier_str oai:localhost:20.500.12123/15366
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network_name_str INTA Digital (INTA)
spelling Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid proteinDistefano, Ana JuliaMaldonado, Sara BeatrizHopp, Horacio EstebanDel Vas, MarianaFijivirusMaízProteínas EstructuralesMaizeStructural ProteinsMal de Río Cuarto VirusCapsidVirus del Mal de Río CuartoCápsideMal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications, we positively identified five structural proteins of approximately 170, 140, 130, 66, and 62 kDa. The protein encoded by MRCV S3 genomic segment was expressed as a fusion protein in Escherichia coli, purified and used for rabbit immunization. The resulting antiserum reacted with the 140 kDa structural protein and with incomplete virus particles in immunoelectron microscopy assays, suggesting that MRCV S3 codes for the major core capsid protein.Instituto de BiotecnologíaFil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Distefano, Ana Julia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Maldonado, Sara Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaSpringer2023-09-28T15:19:34Z2023-09-28T15:19:34Z2009-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15366https://link.springer.com/article/10.1007/s11262-009-0345-21572-994Xhttps://doi.org/10.1007/s11262-009-0345-2Virus Genes 38 (3) : 455-460 (Junio 2009)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-11T10:24:43Zoai:localhost:20.500.12123/15366instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:24:44.202INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
title Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
spellingShingle Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
Distefano, Ana Julia
Fijivirus
Maíz
Proteínas Estructurales
Maize
Structural Proteins
Mal de Río Cuarto Virus
Capsid
Virus del Mal de Río Cuarto
Cápside
title_short Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
title_full Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
title_fullStr Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
title_full_unstemmed Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
title_sort Mal de Río Cuarto virus (MRCV) genomic segment S3 codes for the major core capsid protein
dc.creator.none.fl_str_mv Distefano, Ana Julia
Maldonado, Sara Beatriz
Hopp, Horacio Esteban
Del Vas, Mariana
author Distefano, Ana Julia
author_facet Distefano, Ana Julia
Maldonado, Sara Beatriz
Hopp, Horacio Esteban
Del Vas, Mariana
author_role author
author2 Maldonado, Sara Beatriz
Hopp, Horacio Esteban
Del Vas, Mariana
author2_role author
author
author
dc.subject.none.fl_str_mv Fijivirus
Maíz
Proteínas Estructurales
Maize
Structural Proteins
Mal de Río Cuarto Virus
Capsid
Virus del Mal de Río Cuarto
Cápside
topic Fijivirus
Maíz
Proteínas Estructurales
Maize
Structural Proteins
Mal de Río Cuarto Virus
Capsid
Virus del Mal de Río Cuarto
Cápside
dc.description.none.fl_txt_mv Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications, we positively identified five structural proteins of approximately 170, 140, 130, 66, and 62 kDa. The protein encoded by MRCV S3 genomic segment was expressed as a fusion protein in Escherichia coli, purified and used for rabbit immunization. The resulting antiserum reacted with the 140 kDa structural protein and with incomplete virus particles in immunoelectron microscopy assays, suggesting that MRCV S3 codes for the major core capsid protein.
Instituto de Biotecnología
Fil: Distefano, Ana Julia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Distefano, Ana Julia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Maldonado, Sara Beatriz. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Hopp, Horacio Esteban. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Hopp, Horacio Esteban. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Del Vas, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Mal de Río Cuarto virus (MRCV) is a newly described species of the genus Fijivirus, family Reoviridae. Compared with other plant-infecting genus of the family, the function and localization of MRCV and other Fijivirus proteins are poorly understood. Through analysis of viral particle purifications, we positively identified five structural proteins of approximately 170, 140, 130, 66, and 62 kDa. The protein encoded by MRCV S3 genomic segment was expressed as a fusion protein in Escherichia coli, purified and used for rabbit immunization. The resulting antiserum reacted with the 140 kDa structural protein and with incomplete virus particles in immunoelectron microscopy assays, suggesting that MRCV S3 codes for the major core capsid protein.
publishDate 2009
dc.date.none.fl_str_mv 2009-06
2023-09-28T15:19:34Z
2023-09-28T15:19:34Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15366
https://link.springer.com/article/10.1007/s11262-009-0345-2
1572-994X
https://doi.org/10.1007/s11262-009-0345-2
url http://hdl.handle.net/20.500.12123/15366
https://link.springer.com/article/10.1007/s11262-009-0345-2
https://doi.org/10.1007/s11262-009-0345-2
identifier_str_mv 1572-994X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Virus Genes 38 (3) : 455-460 (Junio 2009)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.993085

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