Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes

Autores
Svetaz, Laura; Bustamante, Claudia Anabel; Goldy, Camila; Rivero, Nery; Müller, Gabriela L.; Valentini, Gabriel Hugo; Fernie, Alisdair R.; Drincovich, María Fabiana; Lara, María Valeria
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Leaf peach curl is a devastating disease affecting leaves, flowers and fruits, caused by the dimorphic fungus Taphrina deformans. To gain insight into the mechanisms of fungus pathogenesis and plant responses, leaves of a resistant and two susceptible Prunus persica genotypes were inoculated with blastospores (yeast), and the infection was monitored during 120 h post inoculation (h.p.i.). Fungal dimorphism to the filamentous form and induction of reactive oxygen species (ROS), callose synthesis, cell death and defence compound production were observed independently of the genotype. Fungal load significantly decreased after 120 h.p.i. in the resistant genotype, while the pathogen tended to grow in the susceptible genotypes. Metabolic profiling revealed a biphasic re‐programming of plant tissue in susceptible genotypes, with an initial stage co‐incident with the yeast form of the fungus and a second when the hypha is developed. Transcriptional analysis of PRs and plant hormone‐related genes indicated that pathogenesis‐related (PR) proteins are involved in P. persica defence responses against T. deformans and that salicylic acid is induced in the resistant genotype. Conducted experiments allowed the elucidation of common and differential responses in susceptible versus resistant genotypes and thus allow us to construct a picture of early events during T. deformans infection.
EEA San Pedro
Fil: Svetaz, Laura A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
Fil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
Fil: Goldy, Camila. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina.
Fil: Rivero, Nery. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
Fil: Müller, Gabriela L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
Fil: Valentini, Gabriel Hugo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina.
Fil: Fernie, Alisdair R. Planck-Institut für Molekulare Pflanzenphysiologie; Alemania
Fil: Drincovich, Maria F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
Fil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
Fuente
Plant, cell and environment 40 (8) : 1456–1473. (2017)
Materia
Relaciones Huesped Patógeno
Taphrina deformans
Prunus Persica
Rizado de las Hojas
Enfermedades de las Plantas
Flavonoides
Ácido Salicílico
Host Pathogen Relations
Leaf Curls
Plant Diseases
Flavonoids
Salicylic Acids
Durazno
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2423
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2423
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network_name_str INTA Digital (INTA)
spelling Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypesSvetaz, LauraBustamante, Claudia AnabelGoldy, CamilaRivero, NeryMüller, Gabriela L.Valentini, Gabriel HugoFernie, Alisdair R.Drincovich, María FabianaLara, María ValeriaRelaciones Huesped PatógenoTaphrina deformansPrunus PersicaRizado de las HojasEnfermedades de las PlantasFlavonoidesÁcido SalicílicoHost Pathogen RelationsLeaf CurlsPlant DiseasesFlavonoidsSalicylic AcidsDuraznoLeaf peach curl is a devastating disease affecting leaves, flowers and fruits, caused by the dimorphic fungus Taphrina deformans. To gain insight into the mechanisms of fungus pathogenesis and plant responses, leaves of a resistant and two susceptible Prunus persica genotypes were inoculated with blastospores (yeast), and the infection was monitored during 120 h post inoculation (h.p.i.). Fungal dimorphism to the filamentous form and induction of reactive oxygen species (ROS), callose synthesis, cell death and defence compound production were observed independently of the genotype. Fungal load significantly decreased after 120 h.p.i. in the resistant genotype, while the pathogen tended to grow in the susceptible genotypes. Metabolic profiling revealed a biphasic re‐programming of plant tissue in susceptible genotypes, with an initial stage co‐incident with the yeast form of the fungus and a second when the hypha is developed. Transcriptional analysis of PRs and plant hormone‐related genes indicated that pathogenesis‐related (PR) proteins are involved in P. persica defence responses against T. deformans and that salicylic acid is induced in the resistant genotype. Conducted experiments allowed the elucidation of common and differential responses in susceptible versus resistant genotypes and thus allow us to construct a picture of early events during T. deformans infection.EEA San PedroFil: Svetaz, Laura A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.Fil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.Fil: Goldy, Camila. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina.Fil: Rivero, Nery. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.Fil: Müller, Gabriela L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.Fil: Valentini, Gabriel Hugo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina.Fil: Fernie, Alisdair R. Planck-Institut für Molekulare Pflanzenphysiologie; AlemaniaFil: Drincovich, Maria F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.Fil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.2018-05-17T17:42:16Z2018-05-17T17:42:16Z2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12942http://hdl.handle.net/20.500.12123/24231365-30400140-7791https://doi.org/10.1111/pce.12942Plant, cell and environment 40 (8) : 1456–1473. (2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-10-16T09:29:10Zoai:localhost:20.500.12123/2423instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:10.813INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes
title Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes
spellingShingle Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes
Svetaz, Laura
Relaciones Huesped Patógeno
Taphrina deformans
Prunus Persica
Rizado de las Hojas
Enfermedades de las Plantas
Flavonoides
Ácido Salicílico
Host Pathogen Relations
Leaf Curls
Plant Diseases
Flavonoids
Salicylic Acids
Durazno
title_short Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes
title_full Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes
title_fullStr Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes
title_full_unstemmed Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes
title_sort Unravelling early events in the Taphrina deformans–Prunus persica interaction: an insight into the differential responses in resistant and susceptible genotypes
dc.creator.none.fl_str_mv Svetaz, Laura
Bustamante, Claudia Anabel
Goldy, Camila
Rivero, Nery
Müller, Gabriela L.
Valentini, Gabriel Hugo
Fernie, Alisdair R.
Drincovich, María Fabiana
Lara, María Valeria
author Svetaz, Laura
author_facet Svetaz, Laura
Bustamante, Claudia Anabel
Goldy, Camila
Rivero, Nery
Müller, Gabriela L.
Valentini, Gabriel Hugo
Fernie, Alisdair R.
Drincovich, María Fabiana
Lara, María Valeria
author_role author
author2 Bustamante, Claudia Anabel
Goldy, Camila
Rivero, Nery
Müller, Gabriela L.
Valentini, Gabriel Hugo
Fernie, Alisdair R.
Drincovich, María Fabiana
Lara, María Valeria
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Relaciones Huesped Patógeno
Taphrina deformans
Prunus Persica
Rizado de las Hojas
Enfermedades de las Plantas
Flavonoides
Ácido Salicílico
Host Pathogen Relations
Leaf Curls
Plant Diseases
Flavonoids
Salicylic Acids
Durazno
topic Relaciones Huesped Patógeno
Taphrina deformans
Prunus Persica
Rizado de las Hojas
Enfermedades de las Plantas
Flavonoides
Ácido Salicílico
Host Pathogen Relations
Leaf Curls
Plant Diseases
Flavonoids
Salicylic Acids
Durazno
dc.description.none.fl_txt_mv Leaf peach curl is a devastating disease affecting leaves, flowers and fruits, caused by the dimorphic fungus Taphrina deformans. To gain insight into the mechanisms of fungus pathogenesis and plant responses, leaves of a resistant and two susceptible Prunus persica genotypes were inoculated with blastospores (yeast), and the infection was monitored during 120 h post inoculation (h.p.i.). Fungal dimorphism to the filamentous form and induction of reactive oxygen species (ROS), callose synthesis, cell death and defence compound production were observed independently of the genotype. Fungal load significantly decreased after 120 h.p.i. in the resistant genotype, while the pathogen tended to grow in the susceptible genotypes. Metabolic profiling revealed a biphasic re‐programming of plant tissue in susceptible genotypes, with an initial stage co‐incident with the yeast form of the fungus and a second when the hypha is developed. Transcriptional analysis of PRs and plant hormone‐related genes indicated that pathogenesis‐related (PR) proteins are involved in P. persica defence responses against T. deformans and that salicylic acid is induced in the resistant genotype. Conducted experiments allowed the elucidation of common and differential responses in susceptible versus resistant genotypes and thus allow us to construct a picture of early events during T. deformans infection.
EEA San Pedro
Fil: Svetaz, Laura A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas.Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
Fil: Bustamante, Claudia A. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
Fil: Goldy, Camila. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina.
Fil: Rivero, Nery. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
Fil: Müller, Gabriela L. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
Fil: Valentini, Gabriel Hugo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina.
Fil: Fernie, Alisdair R. Planck-Institut für Molekulare Pflanzenphysiologie; Alemania
Fil: Drincovich, Maria F. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
Fil: Lara, María Valeria. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina.
description Leaf peach curl is a devastating disease affecting leaves, flowers and fruits, caused by the dimorphic fungus Taphrina deformans. To gain insight into the mechanisms of fungus pathogenesis and plant responses, leaves of a resistant and two susceptible Prunus persica genotypes were inoculated with blastospores (yeast), and the infection was monitored during 120 h post inoculation (h.p.i.). Fungal dimorphism to the filamentous form and induction of reactive oxygen species (ROS), callose synthesis, cell death and defence compound production were observed independently of the genotype. Fungal load significantly decreased after 120 h.p.i. in the resistant genotype, while the pathogen tended to grow in the susceptible genotypes. Metabolic profiling revealed a biphasic re‐programming of plant tissue in susceptible genotypes, with an initial stage co‐incident with the yeast form of the fungus and a second when the hypha is developed. Transcriptional analysis of PRs and plant hormone‐related genes indicated that pathogenesis‐related (PR) proteins are involved in P. persica defence responses against T. deformans and that salicylic acid is induced in the resistant genotype. Conducted experiments allowed the elucidation of common and differential responses in susceptible versus resistant genotypes and thus allow us to construct a picture of early events during T. deformans infection.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018-05-17T17:42:16Z
2018-05-17T17:42:16Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12942
http://hdl.handle.net/20.500.12123/2423
1365-3040
0140-7791
https://doi.org/10.1111/pce.12942
url https://onlinelibrary.wiley.com/doi/abs/10.1111/pce.12942
http://hdl.handle.net/20.500.12123/2423
https://doi.org/10.1111/pce.12942
identifier_str_mv 1365-3040
0140-7791
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Plant, cell and environment 40 (8) : 1456–1473. (2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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