Experimental selection of long‐term intracellular mycobacteria

Autores
Vazquez, Cristina Lourdes; Lerner, Thomas R.; Kasmapour, Bahram; Pei, Gang; Gronow, Achim; Bianco, María Veronica; Blanco, Federico Carlos; Bleck, Christopher K.E.; Geffers, Robert; Bigi, Fabiana; Abraham, Wolf-Rainer; Gutierrez, Maximiliano Gabriel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Some intracellular bacteria are known to cause long‐term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long‐term intracellular lifestyle. Selected Mycobacterium bovis BCG through continuous culture in macrophages underwent an adaptation process leading to impaired phenolic glycolipids (PGL) synthesis, improved usage of glucose as a carbon source and accumulation of neutral lipids. These changes correlated with increased survival of mycobacteria in macrophages and mice during re‐infection and also with the specific expression of stress‐ and survival‐related genes. Our findings identify bacterial traits implicated in the establishment of long‐term cellular infections and represent a tool for understanding the physiological states and the environment that bacteria face living in fluctuating intracellular environments.
Instituto de Biotecnología
Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania
Fil: Lerner, Thomas R. National Institute for Medical Research. Medical Research Council. Division of Mycobacterial Research; Gran Bretaña
Fil: Kasmapour, Bahram. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania
Fil: Pei, Gang. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania
Fil: Gronow, Achim. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania
Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Bleck, Christopher K.E. University of Basel. Centre for Cellular Imaging and NanoAnalytics, Structural Biology and Biophysics, Biozentrum, Mattenstrasse 26; Suiza
Fil: Geffers, Robert. Helmholtz Centre for Infection Research. Research Group Genome Analytics; Alemania
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Abraham, Wolf-Rainer. Helmholtz Centre for Infection Research. Research Group Chemical Microbiology; Alemania
Fil: Gutierrez, Maximiliano Gabriel. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania. National Institute for Medical Research. Medical Research Council. Division of Mycobacterial Research; Gran Bretaña
Fuente
Cellular microbiology 16 (9) : 1425-1440. (September 2014)
Materia
Mycobacterium
Experimentos de Largo Plazo
Líquido Intracellular
Mycobacterium Bovis
Glicolípidos
Supervivencia
Long Term Experiments
Intracellular Fluid
Glycolipids
Survival
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4858
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4858
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Experimental selection of long‐term intracellular mycobacteriaVazquez, Cristina LourdesLerner, Thomas R.Kasmapour, BahramPei, GangGronow, AchimBianco, María VeronicaBlanco, Federico CarlosBleck, Christopher K.E.Geffers, RobertBigi, FabianaAbraham, Wolf-RainerGutierrez, Maximiliano GabrielMycobacteriumExperimentos de Largo PlazoLíquido IntracellularMycobacterium BovisGlicolípidosSupervivenciaLong Term ExperimentsIntracellular FluidGlycolipidsSurvivalSome intracellular bacteria are known to cause long‐term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long‐term intracellular lifestyle. Selected Mycobacterium bovis BCG through continuous culture in macrophages underwent an adaptation process leading to impaired phenolic glycolipids (PGL) synthesis, improved usage of glucose as a carbon source and accumulation of neutral lipids. These changes correlated with increased survival of mycobacteria in macrophages and mice during re‐infection and also with the specific expression of stress‐ and survival‐related genes. Our findings identify bacterial traits implicated in the establishment of long‐term cellular infections and represent a tool for understanding the physiological states and the environment that bacteria face living in fluctuating intracellular environments.Instituto de BiotecnologíaFil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; AlemaniaFil: Lerner, Thomas R. National Institute for Medical Research. Medical Research Council. Division of Mycobacterial Research; Gran BretañaFil: Kasmapour, Bahram. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; AlemaniaFil: Pei, Gang. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; AlemaniaFil: Gronow, Achim. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; AlemaniaFil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Bleck, Christopher K.E. University of Basel. Centre for Cellular Imaging and NanoAnalytics, Structural Biology and Biophysics, Biozentrum, Mattenstrasse 26; SuizaFil: Geffers, Robert. Helmholtz Centre for Infection Research. Research Group Genome Analytics; AlemaniaFil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Abraham, Wolf-Rainer. Helmholtz Centre for Infection Research. Research Group Chemical Microbiology; AlemaniaFil: Gutierrez, Maximiliano Gabriel. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania. National Institute for Medical Research. Medical Research Council. Division of Mycobacterial Research; Gran BretañaWiley2019-04-09T14:25:13Z2019-04-09T14:25:13Z2014-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4858https://onlinelibrary.wiley.com/doi/full/10.1111/cmi.12303#references-section1462-5814https://doi.org/10.1111/cmi.12303Cellular microbiology 16 (9) : 1425-1440. (September 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-12-18T09:01:20Zoai:localhost:20.500.12123/4858instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-12-18 09:01:20.527INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Experimental selection of long‐term intracellular mycobacteria
title Experimental selection of long‐term intracellular mycobacteria
spellingShingle Experimental selection of long‐term intracellular mycobacteria
Vazquez, Cristina Lourdes
Mycobacterium
Experimentos de Largo Plazo
Líquido Intracellular
Mycobacterium Bovis
Glicolípidos
Supervivencia
Long Term Experiments
Intracellular Fluid
Glycolipids
Survival
title_short Experimental selection of long‐term intracellular mycobacteria
title_full Experimental selection of long‐term intracellular mycobacteria
title_fullStr Experimental selection of long‐term intracellular mycobacteria
title_full_unstemmed Experimental selection of long‐term intracellular mycobacteria
title_sort Experimental selection of long‐term intracellular mycobacteria
dc.creator.none.fl_str_mv Vazquez, Cristina Lourdes
Lerner, Thomas R.
Kasmapour, Bahram
Pei, Gang
Gronow, Achim
Bianco, María Veronica
Blanco, Federico Carlos
Bleck, Christopher K.E.
Geffers, Robert
Bigi, Fabiana
Abraham, Wolf-Rainer
Gutierrez, Maximiliano Gabriel
author Vazquez, Cristina Lourdes
author_facet Vazquez, Cristina Lourdes
Lerner, Thomas R.
Kasmapour, Bahram
Pei, Gang
Gronow, Achim
Bianco, María Veronica
Blanco, Federico Carlos
Bleck, Christopher K.E.
Geffers, Robert
Bigi, Fabiana
Abraham, Wolf-Rainer
Gutierrez, Maximiliano Gabriel
author_role author
author2 Lerner, Thomas R.
Kasmapour, Bahram
Pei, Gang
Gronow, Achim
Bianco, María Veronica
Blanco, Federico Carlos
Bleck, Christopher K.E.
Geffers, Robert
Bigi, Fabiana
Abraham, Wolf-Rainer
Gutierrez, Maximiliano Gabriel
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycobacterium
Experimentos de Largo Plazo
Líquido Intracellular
Mycobacterium Bovis
Glicolípidos
Supervivencia
Long Term Experiments
Intracellular Fluid
Glycolipids
Survival
topic Mycobacterium
Experimentos de Largo Plazo
Líquido Intracellular
Mycobacterium Bovis
Glicolípidos
Supervivencia
Long Term Experiments
Intracellular Fluid
Glycolipids
Survival
dc.description.none.fl_txt_mv Some intracellular bacteria are known to cause long‐term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long‐term intracellular lifestyle. Selected Mycobacterium bovis BCG through continuous culture in macrophages underwent an adaptation process leading to impaired phenolic glycolipids (PGL) synthesis, improved usage of glucose as a carbon source and accumulation of neutral lipids. These changes correlated with increased survival of mycobacteria in macrophages and mice during re‐infection and also with the specific expression of stress‐ and survival‐related genes. Our findings identify bacterial traits implicated in the establishment of long‐term cellular infections and represent a tool for understanding the physiological states and the environment that bacteria face living in fluctuating intracellular environments.
Instituto de Biotecnología
Fil: Vazquez, Cristina Lourdes. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania
Fil: Lerner, Thomas R. National Institute for Medical Research. Medical Research Council. Division of Mycobacterial Research; Gran Bretaña
Fil: Kasmapour, Bahram. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania
Fil: Pei, Gang. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania
Fil: Gronow, Achim. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania
Fil: Bianco, María Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Blanco, Federico Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Bleck, Christopher K.E. University of Basel. Centre for Cellular Imaging and NanoAnalytics, Structural Biology and Biophysics, Biozentrum, Mattenstrasse 26; Suiza
Fil: Geffers, Robert. Helmholtz Centre for Infection Research. Research Group Genome Analytics; Alemania
Fil: Bigi, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Abraham, Wolf-Rainer. Helmholtz Centre for Infection Research. Research Group Chemical Microbiology; Alemania
Fil: Gutierrez, Maximiliano Gabriel. Helmholtz Centre for Infection Research. Research Group Phagosome Biology; Alemania. National Institute for Medical Research. Medical Research Council. Division of Mycobacterial Research; Gran Bretaña
description Some intracellular bacteria are known to cause long‐term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long‐term intracellular lifestyle. Selected Mycobacterium bovis BCG through continuous culture in macrophages underwent an adaptation process leading to impaired phenolic glycolipids (PGL) synthesis, improved usage of glucose as a carbon source and accumulation of neutral lipids. These changes correlated with increased survival of mycobacteria in macrophages and mice during re‐infection and also with the specific expression of stress‐ and survival‐related genes. Our findings identify bacterial traits implicated in the establishment of long‐term cellular infections and represent a tool for understanding the physiological states and the environment that bacteria face living in fluctuating intracellular environments.
publishDate 2014
dc.date.none.fl_str_mv 2014-09
2019-04-09T14:25:13Z
2019-04-09T14:25:13Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4858
https://onlinelibrary.wiley.com/doi/full/10.1111/cmi.12303#references-section
1462-5814
https://doi.org/10.1111/cmi.12303
url http://hdl.handle.net/20.500.12123/4858
https://onlinelibrary.wiley.com/doi/full/10.1111/cmi.12303#references-section
https://doi.org/10.1111/cmi.12303
identifier_str_mv 1462-5814
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Cellular microbiology 16 (9) : 1425-1440. (September 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.952241

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