Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses

Autores
Alfonso, Victoria; Lopez, Maria Gabriela; Carrillo, Elisa Cristina; Taboga, Oscar Alberto
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Baculovirus occlusion-derived viruses (ODVs) and budded viruses (BVs) are morphologically and functionally distinct. ODVs are responsible for primary infection in insect hosts because of their high per os infectivity. On the contrary, BVs poorly infect endothelial gut cells, but propagate the infection in the tissues of insects with a high efficiency. P74 is one of the most important proteins from ODVs, and it participates in the attachment of this viral phenotype to endothelial cells in the midgut. We evaluated the possibility of pseudotyping BVs of Autographa californica multiple nucleopolyhedrovirus with two versions of P74 and its effect on their oral infectivity. Both recombinant BVs contained P74 and replicated similarly to wild-type viruses. Nevertheless, the presence of P74 on the BV’s surface does not enhance the oral infectivity of this phenotype, suggesting that the presence of P74 in the membrane of budded virions interferes with their mechanism of infecting midgut cells.
Instituto de Biotecnología
Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.
Fil: Carrillo, Elisa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Intervirology 55 (3) : 247–251. (February 2012)
Materia
Virus
Baculoviridae
Lepidoptera
Autographa californica
Patogenicidad
Fenotipos
Viruses
Pathogenicity
Phenotypes
Poder infeccioso
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5118
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5118
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network_name_str INTA Digital (INTA)
spelling Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded VirusesAlfonso, VictoriaLopez, Maria GabrielaCarrillo, Elisa CristinaTaboga, Oscar AlbertoVirusBaculoviridaeLepidopteraAutographa californicaPatogenicidadFenotiposVirusesPathogenicityPhenotypesPoder infecciosoBaculovirus occlusion-derived viruses (ODVs) and budded viruses (BVs) are morphologically and functionally distinct. ODVs are responsible for primary infection in insect hosts because of their high per os infectivity. On the contrary, BVs poorly infect endothelial gut cells, but propagate the infection in the tissues of insects with a high efficiency. P74 is one of the most important proteins from ODVs, and it participates in the attachment of this viral phenotype to endothelial cells in the midgut. We evaluated the possibility of pseudotyping BVs of Autographa californica multiple nucleopolyhedrovirus with two versions of P74 and its effect on their oral infectivity. Both recombinant BVs contained P74 and replicated similarly to wild-type viruses. Nevertheless, the presence of P74 on the BV’s surface does not enhance the oral infectivity of this phenotype, suggesting that the presence of P74 in the membrane of budded virions interferes with their mechanism of infecting midgut cells.Instituto de BiotecnologíaFil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.Fil: Carrillo, Elisa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaS. Karger AG2019-05-15T13:53:09Z2019-05-15T13:53:09Z2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.karger.com/Article/Abstract/324538http://hdl.handle.net/20.500.12123/51180300-55261423-0100https://doi.org/10.1159/000324538Intervirology 55 (3) : 247–251. (February 2012)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:39Zoai:localhost:20.500.12123/5118instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:40.258INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses
title Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses
spellingShingle Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses
Alfonso, Victoria
Virus
Baculoviridae
Lepidoptera
Autographa californica
Patogenicidad
Fenotipos
Viruses
Pathogenicity
Phenotypes
Poder infeccioso
title_short Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses
title_full Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses
title_fullStr Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses
title_full_unstemmed Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses
title_sort Surface Display of AcMNPV Occlusion-Derived P74 Does Not Enhance Oral Infectivity of Budded Viruses
dc.creator.none.fl_str_mv Alfonso, Victoria
Lopez, Maria Gabriela
Carrillo, Elisa Cristina
Taboga, Oscar Alberto
author Alfonso, Victoria
author_facet Alfonso, Victoria
Lopez, Maria Gabriela
Carrillo, Elisa Cristina
Taboga, Oscar Alberto
author_role author
author2 Lopez, Maria Gabriela
Carrillo, Elisa Cristina
Taboga, Oscar Alberto
author2_role author
author
author
dc.subject.none.fl_str_mv Virus
Baculoviridae
Lepidoptera
Autographa californica
Patogenicidad
Fenotipos
Viruses
Pathogenicity
Phenotypes
Poder infeccioso
topic Virus
Baculoviridae
Lepidoptera
Autographa californica
Patogenicidad
Fenotipos
Viruses
Pathogenicity
Phenotypes
Poder infeccioso
dc.description.none.fl_txt_mv Baculovirus occlusion-derived viruses (ODVs) and budded viruses (BVs) are morphologically and functionally distinct. ODVs are responsible for primary infection in insect hosts because of their high per os infectivity. On the contrary, BVs poorly infect endothelial gut cells, but propagate the infection in the tissues of insects with a high efficiency. P74 is one of the most important proteins from ODVs, and it participates in the attachment of this viral phenotype to endothelial cells in the midgut. We evaluated the possibility of pseudotyping BVs of Autographa californica multiple nucleopolyhedrovirus with two versions of P74 and its effect on their oral infectivity. Both recombinant BVs contained P74 and replicated similarly to wild-type viruses. Nevertheless, the presence of P74 on the BV’s surface does not enhance the oral infectivity of this phenotype, suggesting that the presence of P74 in the membrane of budded virions interferes with their mechanism of infecting midgut cells.
Instituto de Biotecnología
Fil: Alfonso, Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Lopez, Maria Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.
Fil: Carrillo, Elisa Cristina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Taboga, Oscar Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Baculovirus occlusion-derived viruses (ODVs) and budded viruses (BVs) are morphologically and functionally distinct. ODVs are responsible for primary infection in insect hosts because of their high per os infectivity. On the contrary, BVs poorly infect endothelial gut cells, but propagate the infection in the tissues of insects with a high efficiency. P74 is one of the most important proteins from ODVs, and it participates in the attachment of this viral phenotype to endothelial cells in the midgut. We evaluated the possibility of pseudotyping BVs of Autographa californica multiple nucleopolyhedrovirus with two versions of P74 and its effect on their oral infectivity. Both recombinant BVs contained P74 and replicated similarly to wild-type viruses. Nevertheless, the presence of P74 on the BV’s surface does not enhance the oral infectivity of this phenotype, suggesting that the presence of P74 in the membrane of budded virions interferes with their mechanism of infecting midgut cells.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
2019-05-15T13:53:09Z
2019-05-15T13:53:09Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.karger.com/Article/Abstract/324538
http://hdl.handle.net/20.500.12123/5118
0300-5526
1423-0100
https://doi.org/10.1159/000324538
url https://www.karger.com/Article/Abstract/324538
http://hdl.handle.net/20.500.12123/5118
https://doi.org/10.1159/000324538
identifier_str_mv 0300-5526
1423-0100
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv S. Karger AG
publisher.none.fl_str_mv S. Karger AG
dc.source.none.fl_str_mv Intervirology 55 (3) : 247–251. (February 2012)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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