Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide

Autores
Villar, Margarita; Popara, Marina; Mangold, Atilio Jose; Fuente, José de la
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Ticks transmit zoonotic pathogens worldwide. Nevertheless, very little information is available on their genome, transcriptome and proteome. Herein, we characterized the proteome of Amblyomma americanum adults and nymphs because of their role in pathogen transmission and compared the proteome of A. americanum, A. cajennense and A. variegatum adult ticks. We also used de novo sequencing proteomics data for the analysis of the phylogenetic relationships between the three Amblyomma spp. in a proof of concept for phyloproteomics. The results showed that host and tick proteins involved in blood digestion, heme detoxification, development and innate immunity were differentially represented between adults and nymphs. Although these ticks were unfed, over-represented host proteins may supply nutrients during off-host periods. Tick proteins involved in tick attachment, feeding, heat shock response, protease inhibition and heme detoxification were differentially represented between Amblyomma spp., suggesting adaptation processes to biotic and abiotic factors. These results suggested that phyloproteomics might be a useful tool for the phylogenetic analysis of tick species in which sequence data is a limiting factor and demonstrate the possibilities of proteomics studies for the characterization of relevant tick vector species and provide new relevant information to understand the physiology, development and evolution of these tick species.
EEA Rafaela
Fil: Villar, Margarita. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; España
Fil: Popara, Marina. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; España
Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Fuente, José de la. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; España. Oklahoma State University. Center for Veterinary Health Sciences. Department of Veterinary Pathobiology; Estados Unidos
Fuente
Journal of Proteomics 105 : 204-216 (June 2014)
Materia
Amblyomma
Vectores
Zoonosis
Huéspedes
Vectors
Zoonoses
Hosts
Garrapatas
Proteómica
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwideVillar, MargaritaPopara, MarinaMangold, Atilio JoseFuente, José de laAmblyommaVectoresZoonosisHuéspedesVectorsZoonosesHostsGarrapatasProteómicaTicks transmit zoonotic pathogens worldwide. Nevertheless, very little information is available on their genome, transcriptome and proteome. Herein, we characterized the proteome of Amblyomma americanum adults and nymphs because of their role in pathogen transmission and compared the proteome of A. americanum, A. cajennense and A. variegatum adult ticks. We also used de novo sequencing proteomics data for the analysis of the phylogenetic relationships between the three Amblyomma spp. in a proof of concept for phyloproteomics. The results showed that host and tick proteins involved in blood digestion, heme detoxification, development and innate immunity were differentially represented between adults and nymphs. Although these ticks were unfed, over-represented host proteins may supply nutrients during off-host periods. Tick proteins involved in tick attachment, feeding, heat shock response, protease inhibition and heme detoxification were differentially represented between Amblyomma spp., suggesting adaptation processes to biotic and abiotic factors. These results suggested that phyloproteomics might be a useful tool for the phylogenetic analysis of tick species in which sequence data is a limiting factor and demonstrate the possibilities of proteomics studies for the characterization of relevant tick vector species and provide new relevant information to understand the physiology, development and evolution of these tick species.EEA RafaelaFil: Villar, Margarita. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; EspañaFil: Popara, Marina. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; EspañaFil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Fuente, José de la. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; España. Oklahoma State University. Center for Veterinary Health Sciences. Department of Veterinary Pathobiology; Estados Unidos2018-08-10T11:48:28Z2018-08-10T11:48:28Z2014-06-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S1874391913006519http://hdl.handle.net/20.500.12123/30391874-39191876-7737https://doi.org/10.1016/j.jprot.2013.12.016Journal of Proteomics 105 : 204-216 (June 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:23Zoai:localhost:20.500.12123/3039instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:23.957INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide
title Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide
spellingShingle Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide
Villar, Margarita
Amblyomma
Vectores
Zoonosis
Huéspedes
Vectors
Zoonoses
Hosts
Garrapatas
Proteómica
title_short Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide
title_full Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide
title_fullStr Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide
title_full_unstemmed Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide
title_sort Comparative proteomics for the characterization of the most relevant Amblyomma tick species as vectors of zoonotic pathogens worldwide
dc.creator.none.fl_str_mv Villar, Margarita
Popara, Marina
Mangold, Atilio Jose
Fuente, José de la
author Villar, Margarita
author_facet Villar, Margarita
Popara, Marina
Mangold, Atilio Jose
Fuente, José de la
author_role author
author2 Popara, Marina
Mangold, Atilio Jose
Fuente, José de la
author2_role author
author
author
dc.subject.none.fl_str_mv Amblyomma
Vectores
Zoonosis
Huéspedes
Vectors
Zoonoses
Hosts
Garrapatas
Proteómica
topic Amblyomma
Vectores
Zoonosis
Huéspedes
Vectors
Zoonoses
Hosts
Garrapatas
Proteómica
dc.description.none.fl_txt_mv Ticks transmit zoonotic pathogens worldwide. Nevertheless, very little information is available on their genome, transcriptome and proteome. Herein, we characterized the proteome of Amblyomma americanum adults and nymphs because of their role in pathogen transmission and compared the proteome of A. americanum, A. cajennense and A. variegatum adult ticks. We also used de novo sequencing proteomics data for the analysis of the phylogenetic relationships between the three Amblyomma spp. in a proof of concept for phyloproteomics. The results showed that host and tick proteins involved in blood digestion, heme detoxification, development and innate immunity were differentially represented between adults and nymphs. Although these ticks were unfed, over-represented host proteins may supply nutrients during off-host periods. Tick proteins involved in tick attachment, feeding, heat shock response, protease inhibition and heme detoxification were differentially represented between Amblyomma spp., suggesting adaptation processes to biotic and abiotic factors. These results suggested that phyloproteomics might be a useful tool for the phylogenetic analysis of tick species in which sequence data is a limiting factor and demonstrate the possibilities of proteomics studies for the characterization of relevant tick vector species and provide new relevant information to understand the physiology, development and evolution of these tick species.
EEA Rafaela
Fil: Villar, Margarita. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; España
Fil: Popara, Marina. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; España
Fil: Mangold, Atilio Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Fuente, José de la. CSIC-UCLM-JCCM. Instituto de Investigación en Recursos Cinegéticos (IREC). SaBio; España. Oklahoma State University. Center for Veterinary Health Sciences. Department of Veterinary Pathobiology; Estados Unidos
description Ticks transmit zoonotic pathogens worldwide. Nevertheless, very little information is available on their genome, transcriptome and proteome. Herein, we characterized the proteome of Amblyomma americanum adults and nymphs because of their role in pathogen transmission and compared the proteome of A. americanum, A. cajennense and A. variegatum adult ticks. We also used de novo sequencing proteomics data for the analysis of the phylogenetic relationships between the three Amblyomma spp. in a proof of concept for phyloproteomics. The results showed that host and tick proteins involved in blood digestion, heme detoxification, development and innate immunity were differentially represented between adults and nymphs. Although these ticks were unfed, over-represented host proteins may supply nutrients during off-host periods. Tick proteins involved in tick attachment, feeding, heat shock response, protease inhibition and heme detoxification were differentially represented between Amblyomma spp., suggesting adaptation processes to biotic and abiotic factors. These results suggested that phyloproteomics might be a useful tool for the phylogenetic analysis of tick species in which sequence data is a limiting factor and demonstrate the possibilities of proteomics studies for the characterization of relevant tick vector species and provide new relevant information to understand the physiology, development and evolution of these tick species.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-13
2018-08-10T11:48:28Z
2018-08-10T11:48:28Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S1874391913006519
http://hdl.handle.net/20.500.12123/3039
1874-3919
1876-7737
https://doi.org/10.1016/j.jprot.2013.12.016
url https://www.sciencedirect.com/science/article/pii/S1874391913006519
http://hdl.handle.net/20.500.12123/3039
https://doi.org/10.1016/j.jprot.2013.12.016
identifier_str_mv 1874-3919
1876-7737
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Journal of Proteomics 105 : 204-216 (June 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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