Transcriptomic response of male Anastrepha fraterculus (Diptera: Tephritidae) to sexual stimulation by host plant volatiles

Autores
Belliard, Silvina Ahnahi; Hurtado, Juan; Matzkin, Luciano M.; Revale, Santiago; Segura, Diego Fernando
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Anastrepha fraterculus is a major pest of fruits in South America. Previous studies revealed that males exposed to volatiles from guava, a likely ancestral host, intensify courtship behavior and increase mating success. However, the molecular and physiological mechanisms underlying this effect remain unknown. Here, we explore the impact of exposure to guava essential oil volatiles on the gene expression profile of adult male heads through de novo transcriptome assembly and differential expression analysis. We found 678 differentially expressed genes (FDR < 0.05 and |FC| > 2), 308 of which were over-expressed in exposed males and 370 in non-exposed males. The exposure treatment affected the transcription of genes putatively involved in mucus-forming, lipid metabolism and neuropeptide processing. Our findings provide the first insights into the molecular mechanisms underlying sexual stimulation in A. fraterculus males triggered by host fruit volatiles.
Instituto de Genética
Fil: Belliard, Silvina Ahnahi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Belliard, Silvina Ahnahi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Belliard, Silvina Ahnahi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hurtado, Juan. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE); Argentina
Fil: Hurtado, Juan. University of Arizona. Department of Entomology; Estados Unidos
Fil: Matzkin, Luciano M. University of Arizona. Department of Entomology; Estados Unidos
Fil: Revale, Santiago. Alchemab Therapeutics Ltd.; Reino Unido
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Segura, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics 55 : 101495 (September 2025)
Materia
Sexual Behaviour
Tephritidae
RNA Sequence
Pheromones
Host Plants
Comportamiento Sexual
Secuencia de ARN
Psidium guajava
Feromonas
Anastrepha fraterculus
Plantas Huéspedes
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/22086
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oai_identifier_str oai:localhost:20.500.12123/22086
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network_name_str INTA Digital (INTA)
spelling Transcriptomic response of male Anastrepha fraterculus (Diptera: Tephritidae) to sexual stimulation by host plant volatilesBelliard, Silvina AhnahiHurtado, JuanMatzkin, Luciano M.Revale, SantiagoSegura, Diego FernandoSexual BehaviourTephritidaeRNA SequencePheromonesHost PlantsComportamiento SexualSecuencia de ARNPsidium guajavaFeromonasAnastrepha fraterculusPlantas HuéspedesAnastrepha fraterculus is a major pest of fruits in South America. Previous studies revealed that males exposed to volatiles from guava, a likely ancestral host, intensify courtship behavior and increase mating success. However, the molecular and physiological mechanisms underlying this effect remain unknown. Here, we explore the impact of exposure to guava essential oil volatiles on the gene expression profile of adult male heads through de novo transcriptome assembly and differential expression analysis. We found 678 differentially expressed genes (FDR < 0.05 and |FC| > 2), 308 of which were over-expressed in exposed males and 370 in non-exposed males. The exposure treatment affected the transcription of genes putatively involved in mucus-forming, lipid metabolism and neuropeptide processing. Our findings provide the first insights into the molecular mechanisms underlying sexual stimulation in A. fraterculus males triggered by host fruit volatiles.Instituto de GenéticaFil: Belliard, Silvina Ahnahi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Belliard, Silvina Ahnahi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Belliard, Silvina Ahnahi. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Hurtado, Juan. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE); ArgentinaFil: Hurtado, Juan. University of Arizona. Department of Entomology; Estados UnidosFil: Matzkin, Luciano M. University of Arizona. Department of Entomology; Estados UnidosFil: Revale, Santiago. Alchemab Therapeutics Ltd.; Reino UnidoFil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; ArgentinaFil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Segura, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2025-04-29T10:02:33Z2025-04-29T10:02:33Z2025-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/22086https://www.sciencedirect.com/science/article/abs/pii/S1744117X250008381878-04071744-117Xhttps://doi.org/10.1016/j.cbd.2025.101495Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics 55 : 101495 (September 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:15Zoai:localhost:20.500.12123/22086instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:16.164INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Transcriptomic response of male Anastrepha fraterculus (Diptera: Tephritidae) to sexual stimulation by host plant volatiles
title Transcriptomic response of male Anastrepha fraterculus (Diptera: Tephritidae) to sexual stimulation by host plant volatiles
spellingShingle Transcriptomic response of male Anastrepha fraterculus (Diptera: Tephritidae) to sexual stimulation by host plant volatiles
Belliard, Silvina Ahnahi
Sexual Behaviour
Tephritidae
RNA Sequence
Pheromones
Host Plants
Comportamiento Sexual
Secuencia de ARN
Psidium guajava
Feromonas
Anastrepha fraterculus
Plantas Huéspedes
title_short Transcriptomic response of male Anastrepha fraterculus (Diptera: Tephritidae) to sexual stimulation by host plant volatiles
title_full Transcriptomic response of male Anastrepha fraterculus (Diptera: Tephritidae) to sexual stimulation by host plant volatiles
title_fullStr Transcriptomic response of male Anastrepha fraterculus (Diptera: Tephritidae) to sexual stimulation by host plant volatiles
title_full_unstemmed Transcriptomic response of male Anastrepha fraterculus (Diptera: Tephritidae) to sexual stimulation by host plant volatiles
title_sort Transcriptomic response of male Anastrepha fraterculus (Diptera: Tephritidae) to sexual stimulation by host plant volatiles
dc.creator.none.fl_str_mv Belliard, Silvina Ahnahi
Hurtado, Juan
Matzkin, Luciano M.
Revale, Santiago
Segura, Diego Fernando
author Belliard, Silvina Ahnahi
author_facet Belliard, Silvina Ahnahi
Hurtado, Juan
Matzkin, Luciano M.
Revale, Santiago
Segura, Diego Fernando
author_role author
author2 Hurtado, Juan
Matzkin, Luciano M.
Revale, Santiago
Segura, Diego Fernando
author2_role author
author
author
author
dc.subject.none.fl_str_mv Sexual Behaviour
Tephritidae
RNA Sequence
Pheromones
Host Plants
Comportamiento Sexual
Secuencia de ARN
Psidium guajava
Feromonas
Anastrepha fraterculus
Plantas Huéspedes
topic Sexual Behaviour
Tephritidae
RNA Sequence
Pheromones
Host Plants
Comportamiento Sexual
Secuencia de ARN
Psidium guajava
Feromonas
Anastrepha fraterculus
Plantas Huéspedes
dc.description.none.fl_txt_mv Anastrepha fraterculus is a major pest of fruits in South America. Previous studies revealed that males exposed to volatiles from guava, a likely ancestral host, intensify courtship behavior and increase mating success. However, the molecular and physiological mechanisms underlying this effect remain unknown. Here, we explore the impact of exposure to guava essential oil volatiles on the gene expression profile of adult male heads through de novo transcriptome assembly and differential expression analysis. We found 678 differentially expressed genes (FDR < 0.05 and |FC| > 2), 308 of which were over-expressed in exposed males and 370 in non-exposed males. The exposure treatment affected the transcription of genes putatively involved in mucus-forming, lipid metabolism and neuropeptide processing. Our findings provide the first insights into the molecular mechanisms underlying sexual stimulation in A. fraterculus males triggered by host fruit volatiles.
Instituto de Genética
Fil: Belliard, Silvina Ahnahi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Belliard, Silvina Ahnahi. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Belliard, Silvina Ahnahi. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Hurtado, Juan. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Instituto de Fisiología, Biología Molecular y Neurociencias (IFIBYNE); Argentina
Fil: Hurtado, Juan. University of Arizona. Department of Entomology; Estados Unidos
Fil: Matzkin, Luciano M. University of Arizona. Department of Entomology; Estados Unidos
Fil: Revale, Santiago. Alchemab Therapeutics Ltd.; Reino Unido
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina
Fil: Segura, Diego Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Segura, Diego Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Anastrepha fraterculus is a major pest of fruits in South America. Previous studies revealed that males exposed to volatiles from guava, a likely ancestral host, intensify courtship behavior and increase mating success. However, the molecular and physiological mechanisms underlying this effect remain unknown. Here, we explore the impact of exposure to guava essential oil volatiles on the gene expression profile of adult male heads through de novo transcriptome assembly and differential expression analysis. We found 678 differentially expressed genes (FDR < 0.05 and |FC| > 2), 308 of which were over-expressed in exposed males and 370 in non-exposed males. The exposure treatment affected the transcription of genes putatively involved in mucus-forming, lipid metabolism and neuropeptide processing. Our findings provide the first insights into the molecular mechanisms underlying sexual stimulation in A. fraterculus males triggered by host fruit volatiles.
publishDate 2025
dc.date.none.fl_str_mv 2025-04-29T10:02:33Z
2025-04-29T10:02:33Z
2025-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/22086
https://www.sciencedirect.com/science/article/abs/pii/S1744117X25000838
1878-0407
1744-117X
https://doi.org/10.1016/j.cbd.2025.101495
url http://hdl.handle.net/20.500.12123/22086
https://www.sciencedirect.com/science/article/abs/pii/S1744117X25000838
https://doi.org/10.1016/j.cbd.2025.101495
identifier_str_mv 1878-0407
1744-117X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Comparative Biochemistry and Physiology - Part D: Genomics and Proteomics 55 : 101495 (September 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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