Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence

Autores
Delpiazzo, Rafael; Barcellos, Maila; Barros, Sofia; Bentacor, Laura; Fraga, Martin; Gil, Jorge; Iraola, Gregorio; Morsella, Claudia Graciela; Paolicchi, Fernando; Perez, Ruben; Riet Correa, Franklin; Sanguinetti, Margarita; Silva Silveira, Caroline da; Caballeros, Lucía
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow's medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%–100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity.
EEA Balcarce
Fil: Delpiazzo, Rafael. Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni"; Uruguay.
Fil: Barcellos, Maila. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.
Fil: Barros, Sofía. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.
Fil: Bentacor, Laura. Universidad de la República. Facultad de Medicina; Uruguay.
Fil: Fraga, Martín. Instituto Nacional de Investigación Agropecuaria. Estación Experimental La Estanzuela; Uruguay.
Fil: Gil, Jorge. Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni"; Uruguay.
Fil: Iraola, Gregorio. Institut Pasteur de Montevideo. Laboratorio de Genómica Microbiana; Uruguay. Universidad Mayor. Facultad de Ciencias; Chile. Wellcome Genome Campus, Wellcome Sanger Institute; United Kingdom.
Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Pérez, Ruben. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.
Fil: Riet-Correa, Franklin. Instituto Nacional de Investigación Agropecuaria. Estación Experimental La Estanzuela; Uruguay.
Fil: Sanguinetti, Margarita. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.
Fil: Silva, Alfonso. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.
Fil: Silva Silveira, Caroline da. Instituto Nacional de Investigación Agropecuaria. Estación Experimental La Estanzuela; Uruguay.
Fil: Caballeros, Lucía. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.
Fuente
Veterinary and Animal Science 11 : 100163 (March 2021)
Materia
Ganado Bovino
Toro
Diagnóstico
PCR
Campylobacter Fetus
Campilobacteriosis
Cattle
Bulls
Diagnosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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network_name_str INTA Digital (INTA)
spelling Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequenceDelpiazzo, RafaelBarcellos, MailaBarros, SofiaBentacor, LauraFraga, MartinGil, JorgeIraola, GregorioMorsella, Claudia GracielaPaolicchi, FernandoPerez, RubenRiet Correa, FranklinSanguinetti, MargaritaSilva Silveira, Caroline daCaballeros, LucíaGanado BovinoToroDiagnósticoPCRCampylobacter FetusCampilobacteriosisCattleBullsDiagnosisCampylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow's medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%–100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity.EEA BalcarceFil: Delpiazzo, Rafael. Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni"; Uruguay.Fil: Barcellos, Maila. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.Fil: Barros, Sofía. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.Fil: Bentacor, Laura. Universidad de la República. Facultad de Medicina; Uruguay.Fil: Fraga, Martín. Instituto Nacional de Investigación Agropecuaria. Estación Experimental La Estanzuela; Uruguay.Fil: Gil, Jorge. Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni"; Uruguay.Fil: Iraola, Gregorio. Institut Pasteur de Montevideo. Laboratorio de Genómica Microbiana; Uruguay. Universidad Mayor. Facultad de Ciencias; Chile. Wellcome Genome Campus, Wellcome Sanger Institute; United Kingdom.Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Pérez, Ruben. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.Fil: Riet-Correa, Franklin. Instituto Nacional de Investigación Agropecuaria. Estación Experimental La Estanzuela; Uruguay.Fil: Sanguinetti, Margarita. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.Fil: Silva, Alfonso. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.Fil: Silva Silveira, Caroline da. Instituto Nacional de Investigación Agropecuaria. Estación Experimental La Estanzuela; Uruguay.Fil: Caballeros, Lucía. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.Elsevier2021-03-02T11:23:34Z2021-03-02T11:23:34Z2020-12-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8779https://www.sciencedirect.com/science/article/pii/S2451943X203007642451-943Xhttps://doi.org/10.1016/j.vas.2020.100163Veterinary and Animal Science 11 : 100163 (March 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:48:47Zoai:localhost:20.500.12123/8779instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:47.884INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence
title Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence
spellingShingle Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence
Delpiazzo, Rafael
Ganado Bovino
Toro
Diagnóstico
PCR
Campylobacter Fetus
Campilobacteriosis
Cattle
Bulls
Diagnosis
title_short Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence
title_full Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence
title_fullStr Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence
title_full_unstemmed Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence
title_sort Accurate and fast identification of Campylobacter fetus in bulls by real-time PCR targeting a 16S rRNA gene sequence
dc.creator.none.fl_str_mv Delpiazzo, Rafael
Barcellos, Maila
Barros, Sofia
Bentacor, Laura
Fraga, Martin
Gil, Jorge
Iraola, Gregorio
Morsella, Claudia Graciela
Paolicchi, Fernando
Perez, Ruben
Riet Correa, Franklin
Sanguinetti, Margarita
Silva Silveira, Caroline da
Caballeros, Lucía
author Delpiazzo, Rafael
author_facet Delpiazzo, Rafael
Barcellos, Maila
Barros, Sofia
Bentacor, Laura
Fraga, Martin
Gil, Jorge
Iraola, Gregorio
Morsella, Claudia Graciela
Paolicchi, Fernando
Perez, Ruben
Riet Correa, Franklin
Sanguinetti, Margarita
Silva Silveira, Caroline da
Caballeros, Lucía
author_role author
author2 Barcellos, Maila
Barros, Sofia
Bentacor, Laura
Fraga, Martin
Gil, Jorge
Iraola, Gregorio
Morsella, Claudia Graciela
Paolicchi, Fernando
Perez, Ruben
Riet Correa, Franklin
Sanguinetti, Margarita
Silva Silveira, Caroline da
Caballeros, Lucía
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Toro
Diagnóstico
PCR
Campylobacter Fetus
Campilobacteriosis
Cattle
Bulls
Diagnosis
topic Ganado Bovino
Toro
Diagnóstico
PCR
Campylobacter Fetus
Campilobacteriosis
Cattle
Bulls
Diagnosis
dc.description.none.fl_txt_mv Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow's medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%–100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity.
EEA Balcarce
Fil: Delpiazzo, Rafael. Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni"; Uruguay.
Fil: Barcellos, Maila. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.
Fil: Barros, Sofía. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.
Fil: Bentacor, Laura. Universidad de la República. Facultad de Medicina; Uruguay.
Fil: Fraga, Martín. Instituto Nacional de Investigación Agropecuaria. Estación Experimental La Estanzuela; Uruguay.
Fil: Gil, Jorge. Universidad de la República Oriental del Uruguay. Facultad de Veterinaria. Estación Experimental "Dr. Mario A. Cassinoni"; Uruguay.
Fil: Iraola, Gregorio. Institut Pasteur de Montevideo. Laboratorio de Genómica Microbiana; Uruguay. Universidad Mayor. Facultad de Ciencias; Chile. Wellcome Genome Campus, Wellcome Sanger Institute; United Kingdom.
Fil: Morsella, Claudia Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Paolicchi, Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Pérez, Ruben. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.
Fil: Riet-Correa, Franklin. Instituto Nacional de Investigación Agropecuaria. Estación Experimental La Estanzuela; Uruguay.
Fil: Sanguinetti, Margarita. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.
Fil: Silva, Alfonso. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.
Fil: Silva Silveira, Caroline da. Instituto Nacional de Investigación Agropecuaria. Estación Experimental La Estanzuela; Uruguay.
Fil: Caballeros, Lucía. Universidad de la República Oriental del Uruguay. Facultad de Ciencias; Uruguay.
description Campylobacter fetus is an important animal pathogen that causes infectious infertility, embryonic mortality and abortions in cattle and sheep flocks. There are two recognized subspecies related with reproductive disorders in livestock: Campylobacter fetus subsp. fetus (Cff) and Campylobacter fetus subsp. venerealis (Cfv). Rapid and reliable detection of this pathogenic species in bulls is of upmost importance for disease control in dairy and beef herds as they are asymptomatic carriers. The aim of the present work was to assess the performance a real-time PCR (qPCR) method for the diagnosis of Campylobacter fetus in samples from bulls, comparing it with culture and isolation methods. 520 preputial samples were both cultured in Skirrow's medium and analyzed by qPCR. The estimated sensitivity of qPCR was 90.9% (95% CI, 69.4%–100%), and the specificity was 99.4% (95% CI, 98.6% - 100%). The proportion of C. fetus positive individuals was 2.1% by isolation and 2.5% by qPCR. Isolates were identified by biochemical tests as Cfv (n = 9) and Cff (n = 2). Our findings support the use of qPCR for fast and accurate detection of C. fetus directly from field samples of preputial smegma of bulls. The qPCR method showed to be suitable for massive screenings because it can be performed in pooled samples without losing accuracy and sensitivity.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-24
2021-03-02T11:23:34Z
2021-03-02T11:23:34Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8779
https://www.sciencedirect.com/science/article/pii/S2451943X20300764
2451-943X
https://doi.org/10.1016/j.vas.2020.100163
url http://hdl.handle.net/20.500.12123/8779
https://www.sciencedirect.com/science/article/pii/S2451943X20300764
https://doi.org/10.1016/j.vas.2020.100163
identifier_str_mv 2451-943X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary and Animal Science 11 : 100163 (March 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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