Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils

Autores
Hernandez Guijarro, Keren; Covacevich, Fernanda; Caruso, Graciela; Castro, Dario Carlos; Feingold, Sergio Enrique
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Agriculture in the Argentine Puna faces significant challenges due to adverse climatic conditions and poor sandy soils prone to erosion and nutrient loss, particularly nitrogen (N), which is essential for plant growth. Despite these challenges, there is limited information on the role of beneficial soil microorganisms in nutrient cycling and uptake in this extreme environment. This study aimed to investigate the impact of agricultural practices on N-cycling microorganisms and arbuscular mycorrhizal fungi (AMF) interactions in Abra Pampa soils, located 3487 m above sea level. We conducted three annual samplings during the summers of 2020, 2021, and 2022 across four treatments: two agricultural plots (A1 and A2) amended with llama manure, each under different crop rotations - A1: Andean potato-quinoa-mallow and A2: quinoa-triticale-mallow; one soil under decomposing llama manure (BG) adjacent to agricultural plots; and a pristine control soil covered with native vegetation. We quantified the N-cycling genes (nifH, amoA from Archaea (AOA) and Bacteria (AOB), nirK, nirS and nosZ), and assessed AMF functional activity (percentage of colonization, arbuscule formation and number of spores in soil) and AMF diversity (Glomeraceae family) using PCR-SSCP analysis. Climate conditions were found to significantly influence the abundance of denitrification genes (nirK and nosZ) and AMF functional parameters, correlating with soil ammonia and nitrate concentrations. Variations in nifH abundance and the AOA/AOB ratio were associated with agricultural practices and soil parameters such as ammonia and available phosphorus content, respectively. Notably, associations between N-cycling bacteria and AMF functions suggest synergistic rather than antagonistic interactions between these groups. Agricultural activities were found to adversely affect AMF genetic diversity. The BG site emerged as a hotspot with a significant abundance of denitrification genes, warranting further investigation in future studies. This study provides valuable insights into the potential interactions among microorganisms involved in N cycling and AMF under various land use scenarios in the Argentine Puna, highlighting considerations for developing sustainable agricultural strategies in the region.
EEA Balcarce
Fil: Hernández Guijarro, Keren. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Hernández Guijarro, Keren. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Covacevich, Fernanada. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina.
Fil: Caruso, Graciela. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Castro, Dario Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Abra Pampa; Argentina
Fil: Feingold, Sergio Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Feingold, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fuente
Applied Soil Ecology 216 : 106542. (December 2025)
Materia
Agricultura
Prácticas Agrícolas
Suelo
Nitrógeno
Utilización de la Tierra
Agriculture
Agricultural Practices
Soil
Nitrogen
Mycorrhizae
Land Use
Región Puna Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24522
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oai_identifier_str oai:localhost:20.500.12123/24522
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network_name_str INTA Digital (INTA)
spelling Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soilsHernandez Guijarro, KerenCovacevich, FernandaCaruso, GracielaCastro, Dario CarlosFeingold, Sergio EnriqueAgriculturaPrácticas AgrícolasSueloNitrógenoUtilización de la TierraAgricultureAgricultural PracticesSoilNitrogenMycorrhizaeLand UseRegión Puna ArgentinaAgriculture in the Argentine Puna faces significant challenges due to adverse climatic conditions and poor sandy soils prone to erosion and nutrient loss, particularly nitrogen (N), which is essential for plant growth. Despite these challenges, there is limited information on the role of beneficial soil microorganisms in nutrient cycling and uptake in this extreme environment. This study aimed to investigate the impact of agricultural practices on N-cycling microorganisms and arbuscular mycorrhizal fungi (AMF) interactions in Abra Pampa soils, located 3487 m above sea level. We conducted three annual samplings during the summers of 2020, 2021, and 2022 across four treatments: two agricultural plots (A1 and A2) amended with llama manure, each under different crop rotations - A1: Andean potato-quinoa-mallow and A2: quinoa-triticale-mallow; one soil under decomposing llama manure (BG) adjacent to agricultural plots; and a pristine control soil covered with native vegetation. We quantified the N-cycling genes (nifH, amoA from Archaea (AOA) and Bacteria (AOB), nirK, nirS and nosZ), and assessed AMF functional activity (percentage of colonization, arbuscule formation and number of spores in soil) and AMF diversity (Glomeraceae family) using PCR-SSCP analysis. Climate conditions were found to significantly influence the abundance of denitrification genes (nirK and nosZ) and AMF functional parameters, correlating with soil ammonia and nitrate concentrations. Variations in nifH abundance and the AOA/AOB ratio were associated with agricultural practices and soil parameters such as ammonia and available phosphorus content, respectively. Notably, associations between N-cycling bacteria and AMF functions suggest synergistic rather than antagonistic interactions between these groups. Agricultural activities were found to adversely affect AMF genetic diversity. The BG site emerged as a hotspot with a significant abundance of denitrification genes, warranting further investigation in future studies. This study provides valuable insights into the potential interactions among microorganisms involved in N cycling and AMF under various land use scenarios in the Argentine Puna, highlighting considerations for developing sustainable agricultural strategies in the region.EEA BalcarceFil: Hernández Guijarro, Keren. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaFil: Hernández Guijarro, Keren. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaFil: Covacevich, Fernanada. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina.Fil: Caruso, Graciela. Universidad Nacional de Salta. Facultad de Ciencias Naturales; ArgentinaFil: Castro, Dario Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Abra Pampa; ArgentinaFil: Feingold, Sergio Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.Fil: Feingold, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; ArgentinaElsevier2025-11-10T12:56:18Z2025-11-10T12:56:18Z2025-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24522https://www.sciencedirect.com/science/article/abs/pii/S09291393250068080929-13931873-0272https://doi.org/10.1016/j.apsoil.2025.106542Applied Soil Ecology 216 : 106542. (December 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E2-I037-002, Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso sueloinfo:eu-repograntAgreement/INTA/2023-PD-L03-I093, Fertilidad química, física y biológica del suelo: estrategias para mitigar y restaurar procesos de degradacióninfo:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada.info:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-11-13T08:48:48Zoai:localhost:20.500.12123/24522instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-13 08:48:48.763INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils
title Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils
spellingShingle Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils
Hernandez Guijarro, Keren
Agricultura
Prácticas Agrícolas
Suelo
Nitrógeno
Utilización de la Tierra
Agriculture
Agricultural Practices
Soil
Nitrogen
Mycorrhizae
Land Use
Región Puna Argentina
title_short Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils
title_full Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils
title_fullStr Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils
title_full_unstemmed Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils
title_sort Influence of agricultural practices on nitrogen-cycling microbial communities and their interaction with mycorrhizae in Argentine Puna soils
dc.creator.none.fl_str_mv Hernandez Guijarro, Keren
Covacevich, Fernanda
Caruso, Graciela
Castro, Dario Carlos
Feingold, Sergio Enrique
author Hernandez Guijarro, Keren
author_facet Hernandez Guijarro, Keren
Covacevich, Fernanda
Caruso, Graciela
Castro, Dario Carlos
Feingold, Sergio Enrique
author_role author
author2 Covacevich, Fernanda
Caruso, Graciela
Castro, Dario Carlos
Feingold, Sergio Enrique
author2_role author
author
author
author
dc.subject.none.fl_str_mv Agricultura
Prácticas Agrícolas
Suelo
Nitrógeno
Utilización de la Tierra
Agriculture
Agricultural Practices
Soil
Nitrogen
Mycorrhizae
Land Use
Región Puna Argentina
topic Agricultura
Prácticas Agrícolas
Suelo
Nitrógeno
Utilización de la Tierra
Agriculture
Agricultural Practices
Soil
Nitrogen
Mycorrhizae
Land Use
Región Puna Argentina
dc.description.none.fl_txt_mv Agriculture in the Argentine Puna faces significant challenges due to adverse climatic conditions and poor sandy soils prone to erosion and nutrient loss, particularly nitrogen (N), which is essential for plant growth. Despite these challenges, there is limited information on the role of beneficial soil microorganisms in nutrient cycling and uptake in this extreme environment. This study aimed to investigate the impact of agricultural practices on N-cycling microorganisms and arbuscular mycorrhizal fungi (AMF) interactions in Abra Pampa soils, located 3487 m above sea level. We conducted three annual samplings during the summers of 2020, 2021, and 2022 across four treatments: two agricultural plots (A1 and A2) amended with llama manure, each under different crop rotations - A1: Andean potato-quinoa-mallow and A2: quinoa-triticale-mallow; one soil under decomposing llama manure (BG) adjacent to agricultural plots; and a pristine control soil covered with native vegetation. We quantified the N-cycling genes (nifH, amoA from Archaea (AOA) and Bacteria (AOB), nirK, nirS and nosZ), and assessed AMF functional activity (percentage of colonization, arbuscule formation and number of spores in soil) and AMF diversity (Glomeraceae family) using PCR-SSCP analysis. Climate conditions were found to significantly influence the abundance of denitrification genes (nirK and nosZ) and AMF functional parameters, correlating with soil ammonia and nitrate concentrations. Variations in nifH abundance and the AOA/AOB ratio were associated with agricultural practices and soil parameters such as ammonia and available phosphorus content, respectively. Notably, associations between N-cycling bacteria and AMF functions suggest synergistic rather than antagonistic interactions between these groups. Agricultural activities were found to adversely affect AMF genetic diversity. The BG site emerged as a hotspot with a significant abundance of denitrification genes, warranting further investigation in future studies. This study provides valuable insights into the potential interactions among microorganisms involved in N cycling and AMF under various land use scenarios in the Argentine Puna, highlighting considerations for developing sustainable agricultural strategies in the region.
EEA Balcarce
Fil: Hernández Guijarro, Keren. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Hernández Guijarro, Keren. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
Fil: Covacevich, Fernanada. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Investigaciones en Biodiversidad y Biotecnología; Argentina.
Fil: Caruso, Graciela. Universidad Nacional de Salta. Facultad de Ciencias Naturales; Argentina
Fil: Castro, Dario Carlos. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Abra Pampa; Argentina
Fil: Feingold, Sergio Enrique. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Feingold, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina
description Agriculture in the Argentine Puna faces significant challenges due to adverse climatic conditions and poor sandy soils prone to erosion and nutrient loss, particularly nitrogen (N), which is essential for plant growth. Despite these challenges, there is limited information on the role of beneficial soil microorganisms in nutrient cycling and uptake in this extreme environment. This study aimed to investigate the impact of agricultural practices on N-cycling microorganisms and arbuscular mycorrhizal fungi (AMF) interactions in Abra Pampa soils, located 3487 m above sea level. We conducted three annual samplings during the summers of 2020, 2021, and 2022 across four treatments: two agricultural plots (A1 and A2) amended with llama manure, each under different crop rotations - A1: Andean potato-quinoa-mallow and A2: quinoa-triticale-mallow; one soil under decomposing llama manure (BG) adjacent to agricultural plots; and a pristine control soil covered with native vegetation. We quantified the N-cycling genes (nifH, amoA from Archaea (AOA) and Bacteria (AOB), nirK, nirS and nosZ), and assessed AMF functional activity (percentage of colonization, arbuscule formation and number of spores in soil) and AMF diversity (Glomeraceae family) using PCR-SSCP analysis. Climate conditions were found to significantly influence the abundance of denitrification genes (nirK and nosZ) and AMF functional parameters, correlating with soil ammonia and nitrate concentrations. Variations in nifH abundance and the AOA/AOB ratio were associated with agricultural practices and soil parameters such as ammonia and available phosphorus content, respectively. Notably, associations between N-cycling bacteria and AMF functions suggest synergistic rather than antagonistic interactions between these groups. Agricultural activities were found to adversely affect AMF genetic diversity. The BG site emerged as a hotspot with a significant abundance of denitrification genes, warranting further investigation in future studies. This study provides valuable insights into the potential interactions among microorganisms involved in N cycling and AMF under various land use scenarios in the Argentine Puna, highlighting considerations for developing sustainable agricultural strategies in the region.
publishDate 2025
dc.date.none.fl_str_mv 2025-11-10T12:56:18Z
2025-11-10T12:56:18Z
2025-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24522
https://www.sciencedirect.com/science/article/abs/pii/S0929139325006808
0929-1393
1873-0272
https://doi.org/10.1016/j.apsoil.2025.106542
url http://hdl.handle.net/20.500.12123/24522
https://www.sciencedirect.com/science/article/abs/pii/S0929139325006808
https://doi.org/10.1016/j.apsoil.2025.106542
identifier_str_mv 0929-1393
1873-0272
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E2-I037-002, Biodiversidad edáfica: componente clave para una gestión integral y sustentable del recurso suelo
info:eu-repograntAgreement/INTA/2023-PD-L03-I093, Fertilidad química, física y biológica del suelo: estrategias para mitigar y restaurar procesos de degradación
info:eu-repograntAgreement/INTA/2023-PD-L01-I087, Caracterización de la diversidad genética de plantas, animales y microorganismos mediante herramientas de genómica aplicada.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Applied Soil Ecology 216 : 106542. (December 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.24909

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