On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata)

Autores
Mola, Liliana M.; Vrbová, Iva; Tosto, Daniela Sandra; Zrzavá, Magda; Marec, František
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Odonata have holokinetic chromosomes. About 95% of species have an XX/X0 sex chromosome system, with heterogametic males. There are species with neo-XX/neo-XY sex chromosomes resulting from an X chromosome/autosome fusion. The genus Rhionaeschna includes 42 species found in the Americas. We analyzed the distribution of the nucleolar organizer region (NOR) using FISH with rDNA probes in Rhionaeschna bonariensis (n = 12 + neo-XY), R. planaltica (n = 7 + neo-XY), and Aeshna cyanea (n = 13 + X0). In R. bonariensis and A. cyanea, the NOR is located on a large pair of autosomes, which have a secondary constriction in the latter species. In R. planaltica, the NOR is located on the ancestral part of the neo-X chromosome. Meiotic analysis and FISH results in R. planaltica led to the conclusion that the neo-XY system arose by insertion of the ancestral X chromosome into an autosome. Genomic in situ hybridization, performed for the first time in Odonata, highlighted the entire neo-Y chromosome in meiosis of R. bonariensis, suggesting that it consists mainly of repetitive DNA. This feature and the terminal chiasma localization suggest an ancient origin of the neo-XY system. Our study provides new information on the origin and evolution of neo-sex chromosomes in Odonata, including new types of chromosomal rearrangements, NOR transposition, and heterochromatin accumulation.
Instituto de Biotecnología
Fil: Mola, Liliana M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Laboratorio de Citogenética y Evolución; Argentina
Fil: Mola, Liliana M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vrbová, Iva. Biology Centre CAS. Institute of Entomology; República Checa
Fil: Vrbová, Iva. Biology Centre CAS. Institute of Plant Molecular Biology; República Checa
Fil: Tosto, Daniela Sandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Tosto, Daniela Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zrzavá, Magda. Biology Centre CAS. Institute of Entomology; República Checa
Fil: Zrzavá, Magda. University of South Bohemia. Faculty of Science. Department of Molecular Biology and Genetics; República Checa
Fil: Marec, Frantisek. Biology Centre CAS. Institute of Entomology; República Checa
Fuente
Insects 13 (12) : 1159 (Diciembre 2022)
Materia
Anisoptera (Insecta)
Ribosomal DNA
Meiosis
ADN Ribosomal
Rhionaeschna bonariensis
Aeshnidae
Evolución de los cromosomas neosexuales
Neo-sex Chromosome Evolution
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15488
Acceder
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oai_identifier_str oai:localhost:20.500.12123/15488
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network_name_str INTA Digital (INTA)
spelling On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata)Mola, Liliana M.Vrbová, IvaTosto, Daniela SandraZrzavá, MagdaMarec, FrantišekAnisoptera (Insecta)Ribosomal DNAMeiosisADN RibosomalRhionaeschna bonariensisAeshnidaeEvolución de los cromosomas neosexualesNeo-sex Chromosome EvolutionOdonata have holokinetic chromosomes. About 95% of species have an XX/X0 sex chromosome system, with heterogametic males. There are species with neo-XX/neo-XY sex chromosomes resulting from an X chromosome/autosome fusion. The genus Rhionaeschna includes 42 species found in the Americas. We analyzed the distribution of the nucleolar organizer region (NOR) using FISH with rDNA probes in Rhionaeschna bonariensis (n = 12 + neo-XY), R. planaltica (n = 7 + neo-XY), and Aeshna cyanea (n = 13 + X0). In R. bonariensis and A. cyanea, the NOR is located on a large pair of autosomes, which have a secondary constriction in the latter species. In R. planaltica, the NOR is located on the ancestral part of the neo-X chromosome. Meiotic analysis and FISH results in R. planaltica led to the conclusion that the neo-XY system arose by insertion of the ancestral X chromosome into an autosome. Genomic in situ hybridization, performed for the first time in Odonata, highlighted the entire neo-Y chromosome in meiosis of R. bonariensis, suggesting that it consists mainly of repetitive DNA. This feature and the terminal chiasma localization suggest an ancient origin of the neo-XY system. Our study provides new information on the origin and evolution of neo-sex chromosomes in Odonata, including new types of chromosomal rearrangements, NOR transposition, and heterochromatin accumulation.Instituto de BiotecnologíaFil: Mola, Liliana M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Laboratorio de Citogenética y Evolución; ArgentinaFil: Mola, Liliana M. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Vrbová, Iva. Biology Centre CAS. Institute of Entomology; República ChecaFil: Vrbová, Iva. Biology Centre CAS. Institute of Plant Molecular Biology; República ChecaFil: Tosto, Daniela Sandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Tosto, Daniela Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zrzavá, Magda. Biology Centre CAS. Institute of Entomology; República ChecaFil: Zrzavá, Magda. University of South Bohemia. Faculty of Science. Department of Molecular Biology and Genetics; República ChecaFil: Marec, Frantisek. Biology Centre CAS. Institute of Entomology; República ChecaMDPI2023-10-10T10:10:59Z2023-10-10T10:10:59Z2022-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/15488https://www.mdpi.com/2075-4450/13/12/11592075-4450https://doi.org/10.3390/insects13121159Insects 13 (12) : 1159 (Diciembre 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-11T10:24:45Zoai:localhost:20.500.12123/15488instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-11 10:24:46.135INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata)
title On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata)
spellingShingle On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata)
Mola, Liliana M.
Anisoptera (Insecta)
Ribosomal DNA
Meiosis
ADN Ribosomal
Rhionaeschna bonariensis
Aeshnidae
Evolución de los cromosomas neosexuales
Neo-sex Chromosome Evolution
title_short On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata)
title_full On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata)
title_fullStr On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata)
title_full_unstemmed On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata)
title_sort On the origin of neo-sex chromosomes in the neotropical dragonflies Rhionaeschna bonariensis and R. planaltica (Aeshnidae, Odonata)
dc.creator.none.fl_str_mv Mola, Liliana M.
Vrbová, Iva
Tosto, Daniela Sandra
Zrzavá, Magda
Marec, František
author Mola, Liliana M.
author_facet Mola, Liliana M.
Vrbová, Iva
Tosto, Daniela Sandra
Zrzavá, Magda
Marec, František
author_role author
author2 Vrbová, Iva
Tosto, Daniela Sandra
Zrzavá, Magda
Marec, František
author2_role author
author
author
author
dc.subject.none.fl_str_mv Anisoptera (Insecta)
Ribosomal DNA
Meiosis
ADN Ribosomal
Rhionaeschna bonariensis
Aeshnidae
Evolución de los cromosomas neosexuales
Neo-sex Chromosome Evolution
topic Anisoptera (Insecta)
Ribosomal DNA
Meiosis
ADN Ribosomal
Rhionaeschna bonariensis
Aeshnidae
Evolución de los cromosomas neosexuales
Neo-sex Chromosome Evolution
dc.description.none.fl_txt_mv Odonata have holokinetic chromosomes. About 95% of species have an XX/X0 sex chromosome system, with heterogametic males. There are species with neo-XX/neo-XY sex chromosomes resulting from an X chromosome/autosome fusion. The genus Rhionaeschna includes 42 species found in the Americas. We analyzed the distribution of the nucleolar organizer region (NOR) using FISH with rDNA probes in Rhionaeschna bonariensis (n = 12 + neo-XY), R. planaltica (n = 7 + neo-XY), and Aeshna cyanea (n = 13 + X0). In R. bonariensis and A. cyanea, the NOR is located on a large pair of autosomes, which have a secondary constriction in the latter species. In R. planaltica, the NOR is located on the ancestral part of the neo-X chromosome. Meiotic analysis and FISH results in R. planaltica led to the conclusion that the neo-XY system arose by insertion of the ancestral X chromosome into an autosome. Genomic in situ hybridization, performed for the first time in Odonata, highlighted the entire neo-Y chromosome in meiosis of R. bonariensis, suggesting that it consists mainly of repetitive DNA. This feature and the terminal chiasma localization suggest an ancient origin of the neo-XY system. Our study provides new information on the origin and evolution of neo-sex chromosomes in Odonata, including new types of chromosomal rearrangements, NOR transposition, and heterochromatin accumulation.
Instituto de Biotecnología
Fil: Mola, Liliana M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. Laboratorio de Citogenética y Evolución; Argentina
Fil: Mola, Liliana M. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Vrbová, Iva. Biology Centre CAS. Institute of Entomology; República Checa
Fil: Vrbová, Iva. Biology Centre CAS. Institute of Plant Molecular Biology; República Checa
Fil: Tosto, Daniela Sandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Tosto, Daniela Sandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zrzavá, Magda. Biology Centre CAS. Institute of Entomology; República Checa
Fil: Zrzavá, Magda. University of South Bohemia. Faculty of Science. Department of Molecular Biology and Genetics; República Checa
Fil: Marec, Frantisek. Biology Centre CAS. Institute of Entomology; República Checa
description Odonata have holokinetic chromosomes. About 95% of species have an XX/X0 sex chromosome system, with heterogametic males. There are species with neo-XX/neo-XY sex chromosomes resulting from an X chromosome/autosome fusion. The genus Rhionaeschna includes 42 species found in the Americas. We analyzed the distribution of the nucleolar organizer region (NOR) using FISH with rDNA probes in Rhionaeschna bonariensis (n = 12 + neo-XY), R. planaltica (n = 7 + neo-XY), and Aeshna cyanea (n = 13 + X0). In R. bonariensis and A. cyanea, the NOR is located on a large pair of autosomes, which have a secondary constriction in the latter species. In R. planaltica, the NOR is located on the ancestral part of the neo-X chromosome. Meiotic analysis and FISH results in R. planaltica led to the conclusion that the neo-XY system arose by insertion of the ancestral X chromosome into an autosome. Genomic in situ hybridization, performed for the first time in Odonata, highlighted the entire neo-Y chromosome in meiosis of R. bonariensis, suggesting that it consists mainly of repetitive DNA. This feature and the terminal chiasma localization suggest an ancient origin of the neo-XY system. Our study provides new information on the origin and evolution of neo-sex chromosomes in Odonata, including new types of chromosomal rearrangements, NOR transposition, and heterochromatin accumulation.
publishDate 2022
dc.date.none.fl_str_mv 2022-12
2023-10-10T10:10:59Z
2023-10-10T10:10:59Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15488
https://www.mdpi.com/2075-4450/13/12/1159
2075-4450
https://doi.org/10.3390/insects13121159
url http://hdl.handle.net/20.500.12123/15488
https://www.mdpi.com/2075-4450/13/12/1159
https://doi.org/10.3390/insects13121159
identifier_str_mv 2075-4450
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Insects 13 (12) : 1159 (Diciembre 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.993085

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