Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update

Autores
Varela, Gonzalo Matías; Avalos, Vera; Reyna, Pablo Gastón; Laguna, Irma Graciela; Rodriguez Pardina, Patricia
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Three begomoviruses were identified in bean crops in northwestern Argentina: Bean golden mosaic virus (BGMV), Tomato yellow spot virus (ToYSV) and Soybean blistering mosaic virus (SbBMV). Nucleic acid hybridization probes specific for these three begomoviruses were developed, and used to assay field samples. As some of the analysed begomovirus-infected samples did not react with any of the specific probes, we suspected that other viruses were present. DNA of two of these samples P160, and PRCA4, was amplified by rolling circle amplification (RCA), and cloned. Four clones were sequenced by primer walking and nucleotide comparisons established that clones P160SacI and P160ApaI shared 93 and 94% nucleotide identity with DNA-A and DNA-B of Sida golden mosaic Brazil virus (SiGMBRV), respectively. Clone PRCA4.6PstI had 94% sequence identity with DNA-A of Tomato mottle wrinkle virus (ToMoWV), and clone PRCA4.17PstI exhibited 95% nucleotide sequence identity with the DNA-A of Tomato yellow vein streak virus (ToYVS), indicating a mixed infection. Specific probes were designed using part of the common region of the viral genome of the newly identified begomoviruses and were used to test bean samples during the 2014–2016 growing seasons. ToMoWV was the most frequently detected virus. The incidence of the three previously characterized begomoviruses (BGMV, SbBMV and ToYSV) was also assessed, and BGMV was found to exhibit the highest incidence. The diversity of begomoviruses found in bean in our country is remarkable, since, six different species have been detected up to the present.
Instituto de Patología Vegetal
Fil: Varela, Gonzalo Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Avalos, Vera. Poder Judicial de Santa Cruz. Laboratorio Regional de Investigación Forense-Zona Sur; Argentina
Fil: Reyna, Pablo Gastón. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
Australasian plant pathology 47 (4) : 343–350. (July 2018)
Materia
Frijol
Virus de las Plantas
Begomovirus
Phaseolus Vulgaris
Beans
Plant Viruses
Región Noroeste
Argentina
Tomato Mottle Wrinkle Virus
Tomato Yellow Vein Streak Virus
Sida Golden Mosaic Brazil Virus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4739
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4739
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an updateVarela, Gonzalo MatíasAvalos, VeraReyna, Pablo GastónLaguna, Irma GracielaRodriguez Pardina, PatriciaFrijolVirus de las PlantasBegomovirusPhaseolus VulgarisBeansPlant VirusesRegión NoroesteArgentinaTomato Mottle Wrinkle VirusTomato Yellow Vein Streak VirusSida Golden Mosaic Brazil VirusThree begomoviruses were identified in bean crops in northwestern Argentina: Bean golden mosaic virus (BGMV), Tomato yellow spot virus (ToYSV) and Soybean blistering mosaic virus (SbBMV). Nucleic acid hybridization probes specific for these three begomoviruses were developed, and used to assay field samples. As some of the analysed begomovirus-infected samples did not react with any of the specific probes, we suspected that other viruses were present. DNA of two of these samples P160, and PRCA4, was amplified by rolling circle amplification (RCA), and cloned. Four clones were sequenced by primer walking and nucleotide comparisons established that clones P160SacI and P160ApaI shared 93 and 94% nucleotide identity with DNA-A and DNA-B of Sida golden mosaic Brazil virus (SiGMBRV), respectively. Clone PRCA4.6PstI had 94% sequence identity with DNA-A of Tomato mottle wrinkle virus (ToMoWV), and clone PRCA4.17PstI exhibited 95% nucleotide sequence identity with the DNA-A of Tomato yellow vein streak virus (ToYVS), indicating a mixed infection. Specific probes were designed using part of the common region of the viral genome of the newly identified begomoviruses and were used to test bean samples during the 2014–2016 growing seasons. ToMoWV was the most frequently detected virus. The incidence of the three previously characterized begomoviruses (BGMV, SbBMV and ToYSV) was also assessed, and BGMV was found to exhibit the highest incidence. The diversity of begomoviruses found in bean in our country is remarkable, since, six different species have been detected up to the present.Instituto de Patología VegetalFil: Varela, Gonzalo Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; ArgentinaFil: Avalos, Vera. Poder Judicial de Santa Cruz. Laboratorio Regional de Investigación Forense-Zona Sur; ArgentinaFil: Reyna, Pablo Gastón. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaSpringer; Australasian Plant Pathology Society2019-03-26T12:38:55Z2019-03-26T12:38:55Z2018-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4739https://link.springer.com/article/10.1007%2Fs13313-018-0563-y0815-31911448-6032 (Online)https://doi.org/10.1007/s13313-018-0563-yAustralasian plant pathology 47 (4) : 343–350. (July 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina (nation)info:eu-repo/semantics/restrictedAccess2025-09-04T09:47:54Zoai:localhost:20.500.12123/4739instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:54.305INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update
title Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update
spellingShingle Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update
Varela, Gonzalo Matías
Frijol
Virus de las Plantas
Begomovirus
Phaseolus Vulgaris
Beans
Plant Viruses
Región Noroeste
Argentina
Tomato Mottle Wrinkle Virus
Tomato Yellow Vein Streak Virus
Sida Golden Mosaic Brazil Virus
title_short Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update
title_full Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update
title_fullStr Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update
title_full_unstemmed Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update
title_sort Identification, molecular characterization and relative incidence of begomoviruses infecting bean crops in northwestern Argentina: an update
dc.creator.none.fl_str_mv Varela, Gonzalo Matías
Avalos, Vera
Reyna, Pablo Gastón
Laguna, Irma Graciela
Rodriguez Pardina, Patricia
author Varela, Gonzalo Matías
author_facet Varela, Gonzalo Matías
Avalos, Vera
Reyna, Pablo Gastón
Laguna, Irma Graciela
Rodriguez Pardina, Patricia
author_role author
author2 Avalos, Vera
Reyna, Pablo Gastón
Laguna, Irma Graciela
Rodriguez Pardina, Patricia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Frijol
Virus de las Plantas
Begomovirus
Phaseolus Vulgaris
Beans
Plant Viruses
Región Noroeste
Argentina
Tomato Mottle Wrinkle Virus
Tomato Yellow Vein Streak Virus
Sida Golden Mosaic Brazil Virus
topic Frijol
Virus de las Plantas
Begomovirus
Phaseolus Vulgaris
Beans
Plant Viruses
Región Noroeste
Argentina
Tomato Mottle Wrinkle Virus
Tomato Yellow Vein Streak Virus
Sida Golden Mosaic Brazil Virus
dc.description.none.fl_txt_mv Three begomoviruses were identified in bean crops in northwestern Argentina: Bean golden mosaic virus (BGMV), Tomato yellow spot virus (ToYSV) and Soybean blistering mosaic virus (SbBMV). Nucleic acid hybridization probes specific for these three begomoviruses were developed, and used to assay field samples. As some of the analysed begomovirus-infected samples did not react with any of the specific probes, we suspected that other viruses were present. DNA of two of these samples P160, and PRCA4, was amplified by rolling circle amplification (RCA), and cloned. Four clones were sequenced by primer walking and nucleotide comparisons established that clones P160SacI and P160ApaI shared 93 and 94% nucleotide identity with DNA-A and DNA-B of Sida golden mosaic Brazil virus (SiGMBRV), respectively. Clone PRCA4.6PstI had 94% sequence identity with DNA-A of Tomato mottle wrinkle virus (ToMoWV), and clone PRCA4.17PstI exhibited 95% nucleotide sequence identity with the DNA-A of Tomato yellow vein streak virus (ToYVS), indicating a mixed infection. Specific probes were designed using part of the common region of the viral genome of the newly identified begomoviruses and were used to test bean samples during the 2014–2016 growing seasons. ToMoWV was the most frequently detected virus. The incidence of the three previously characterized begomoviruses (BGMV, SbBMV and ToYSV) was also assessed, and BGMV was found to exhibit the highest incidence. The diversity of begomoviruses found in bean in our country is remarkable, since, six different species have been detected up to the present.
Instituto de Patología Vegetal
Fil: Varela, Gonzalo Matías. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones en Ciencias de la Salud. Universidad Nacional de Córdoba. Instituto de Investigaciones en Ciencias de la Salud; Argentina
Fil: Avalos, Vera. Poder Judicial de Santa Cruz. Laboratorio Regional de Investigación Forense-Zona Sur; Argentina
Fil: Reyna, Pablo Gastón. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Concejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Laguna, Irma Graciela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez Pardina, Patricia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description Three begomoviruses were identified in bean crops in northwestern Argentina: Bean golden mosaic virus (BGMV), Tomato yellow spot virus (ToYSV) and Soybean blistering mosaic virus (SbBMV). Nucleic acid hybridization probes specific for these three begomoviruses were developed, and used to assay field samples. As some of the analysed begomovirus-infected samples did not react with any of the specific probes, we suspected that other viruses were present. DNA of two of these samples P160, and PRCA4, was amplified by rolling circle amplification (RCA), and cloned. Four clones were sequenced by primer walking and nucleotide comparisons established that clones P160SacI and P160ApaI shared 93 and 94% nucleotide identity with DNA-A and DNA-B of Sida golden mosaic Brazil virus (SiGMBRV), respectively. Clone PRCA4.6PstI had 94% sequence identity with DNA-A of Tomato mottle wrinkle virus (ToMoWV), and clone PRCA4.17PstI exhibited 95% nucleotide sequence identity with the DNA-A of Tomato yellow vein streak virus (ToYVS), indicating a mixed infection. Specific probes were designed using part of the common region of the viral genome of the newly identified begomoviruses and were used to test bean samples during the 2014–2016 growing seasons. ToMoWV was the most frequently detected virus. The incidence of the three previously characterized begomoviruses (BGMV, SbBMV and ToYSV) was also assessed, and BGMV was found to exhibit the highest incidence. The diversity of begomoviruses found in bean in our country is remarkable, since, six different species have been detected up to the present.
publishDate 2018
dc.date.none.fl_str_mv 2018-07
2019-03-26T12:38:55Z
2019-03-26T12:38:55Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4739
https://link.springer.com/article/10.1007%2Fs13313-018-0563-y
0815-3191
1448-6032 (Online)
https://doi.org/10.1007/s13313-018-0563-y
url http://hdl.handle.net/20.500.12123/4739
https://link.springer.com/article/10.1007%2Fs13313-018-0563-y
https://doi.org/10.1007/s13313-018-0563-y
identifier_str_mv 0815-3191
1448-6032 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina (nation)
dc.publisher.none.fl_str_mv Springer; Australasian Plant Pathology Society
publisher.none.fl_str_mv Springer; Australasian Plant Pathology Society
dc.source.none.fl_str_mv Australasian plant pathology 47 (4) : 343–350. (July 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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