Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina

Autores
Sebastian, Patrick; Lamattina, Daniela; Vaschalde, Paula J.; Flores, Fernando Sebastián; Torrents, Jorgelina; Tarragona, Evelina Luisa
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Field captured specimens of Didelphis albiventris (Didelphimorphia: Didelphidae) from Formosa Province, Argentina, were analysed for possible infection with Babesia species. Whole blood samples of live-trapped D. albiventris were taken, genomic DNA was extracted and a Piroplasmida specific PCR assay targeting a fragment of the 18S rRNA gene was conducted. Positive PCR amplicon were sequenced and obtained DNA partial sequences were located in a phylogenetic context by constructing a maximum-likelihood tree. Two samples were positive and could be identified as Babesia species. Through phylogenetic analyses, both sequences were demonstrated to belong to a group of Babesia sp. associated with marsupials from Brazil and therefore previously defined as the “South American Marsupialia Group” (SAMG) of Babesia species. The results of this study represent the first report of a Piroplasmida species in a marsupial in Argentina. They further give an insight on the circulation of Babesia sp. in D. albiventris that, due to its synanthropic habits, plays an epidemiologically important role as potential hosts for emerging pathogens and ectoparasites of relevance in veterinary medicine.
EEA Rafaela
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Lamattina, Daniela. Instituto Nacional de Medicina Tropical (Misiones); Argentina
Fil: Vaschalde, Paula J. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina
Fil: Vaschalde, Paula J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina
Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba (CIEC); Argentina
Fil: Flores, Fernando Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Tarragona, Evelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Tarragona, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fuente
Veterinary Parasitology: Regional Studies and Reports 58 : 101205. (February 2025)
Materia
Babesia
Didelphis
Identification
Ticks
Identificación
Garrapata
Argentina
Didelphis albiventris
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/21367
Acceder
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oai_identifier_str oai:localhost:20.500.12123/21367
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network_name_str INTA Digital (INTA)
spelling Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from ArgentinaSebastian, PatrickLamattina, DanielaVaschalde, Paula J.Flores, Fernando SebastiánTorrents, JorgelinaTarragona, Evelina LuisaBabesiaDidelphisIdentificationTicksIdentificaciónGarrapataArgentinaDidelphis albiventrisField captured specimens of Didelphis albiventris (Didelphimorphia: Didelphidae) from Formosa Province, Argentina, were analysed for possible infection with Babesia species. Whole blood samples of live-trapped D. albiventris were taken, genomic DNA was extracted and a Piroplasmida specific PCR assay targeting a fragment of the 18S rRNA gene was conducted. Positive PCR amplicon were sequenced and obtained DNA partial sequences were located in a phylogenetic context by constructing a maximum-likelihood tree. Two samples were positive and could be identified as Babesia species. Through phylogenetic analyses, both sequences were demonstrated to belong to a group of Babesia sp. associated with marsupials from Brazil and therefore previously defined as the “South American Marsupialia Group” (SAMG) of Babesia species. The results of this study represent the first report of a Piroplasmida species in a marsupial in Argentina. They further give an insight on the circulation of Babesia sp. in D. albiventris that, due to its synanthropic habits, plays an epidemiologically important role as potential hosts for emerging pathogens and ectoparasites of relevance in veterinary medicine.EEA RafaelaFil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Lamattina, Daniela. Instituto Nacional de Medicina Tropical (Misiones); ArgentinaFil: Vaschalde, Paula J. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; ArgentinaFil: Vaschalde, Paula J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; ArgentinaFil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba (CIEC); ArgentinaFil: Flores, Fernando Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Tarragona, Evelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaFil: Tarragona, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); ArgentinaElsevier2025-02-20T11:39:42Z2025-02-20T11:39:42Z2025-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/21367https://www.sciencedirect.com/science/article/abs/pii/S24059390250001272405-9390https://doi.org/10.1016/j.vprsr.2025.101205Veterinary Parasitology: Regional Studies and Reports 58 : 101205. (February 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y lainfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:50:55Zoai:localhost:20.500.12123/21367instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:50:56.288INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina
title Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina
spellingShingle Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina
Sebastian, Patrick
Babesia
Didelphis
Identification
Ticks
Identificación
Garrapata
Argentina
Didelphis albiventris
title_short Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina
title_full Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina
title_fullStr Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina
title_full_unstemmed Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina
title_sort Babesia sp. of the “South American Marsupialia Group”: First molecular detection in Didelphis albiventris (Didelphimorphia: Didelphidae) from Argentina
dc.creator.none.fl_str_mv Sebastian, Patrick
Lamattina, Daniela
Vaschalde, Paula J.
Flores, Fernando Sebastián
Torrents, Jorgelina
Tarragona, Evelina Luisa
author Sebastian, Patrick
author_facet Sebastian, Patrick
Lamattina, Daniela
Vaschalde, Paula J.
Flores, Fernando Sebastián
Torrents, Jorgelina
Tarragona, Evelina Luisa
author_role author
author2 Lamattina, Daniela
Vaschalde, Paula J.
Flores, Fernando Sebastián
Torrents, Jorgelina
Tarragona, Evelina Luisa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Babesia
Didelphis
Identification
Ticks
Identificación
Garrapata
Argentina
Didelphis albiventris
topic Babesia
Didelphis
Identification
Ticks
Identificación
Garrapata
Argentina
Didelphis albiventris
dc.description.none.fl_txt_mv Field captured specimens of Didelphis albiventris (Didelphimorphia: Didelphidae) from Formosa Province, Argentina, were analysed for possible infection with Babesia species. Whole blood samples of live-trapped D. albiventris were taken, genomic DNA was extracted and a Piroplasmida specific PCR assay targeting a fragment of the 18S rRNA gene was conducted. Positive PCR amplicon were sequenced and obtained DNA partial sequences were located in a phylogenetic context by constructing a maximum-likelihood tree. Two samples were positive and could be identified as Babesia species. Through phylogenetic analyses, both sequences were demonstrated to belong to a group of Babesia sp. associated with marsupials from Brazil and therefore previously defined as the “South American Marsupialia Group” (SAMG) of Babesia species. The results of this study represent the first report of a Piroplasmida species in a marsupial in Argentina. They further give an insight on the circulation of Babesia sp. in D. albiventris that, due to its synanthropic habits, plays an epidemiologically important role as potential hosts for emerging pathogens and ectoparasites of relevance in veterinary medicine.
EEA Rafaela
Fil: Sebastian, Patrick. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Sebastian, Patrick. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Lamattina, Daniela. Instituto Nacional de Medicina Tropical (Misiones); Argentina
Fil: Vaschalde, Paula J. Universidad Nacional del Litoral. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina
Fil: Vaschalde, Paula J. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias Veterinarias del Litoral. Laboratorio de Ecología de Enfermedades; Argentina
Fil: Flores, Fernando Sebastián. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Centro de Investigaciones Entomológicas de Córdoba (CIEC); Argentina
Fil: Flores, Fernando Sebastián. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Torrents, Jorgelina. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Tarragona, Evelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
Fil: Tarragona, Evelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación de la Cadena Láctea (IDICAL); Argentina
description Field captured specimens of Didelphis albiventris (Didelphimorphia: Didelphidae) from Formosa Province, Argentina, were analysed for possible infection with Babesia species. Whole blood samples of live-trapped D. albiventris were taken, genomic DNA was extracted and a Piroplasmida specific PCR assay targeting a fragment of the 18S rRNA gene was conducted. Positive PCR amplicon were sequenced and obtained DNA partial sequences were located in a phylogenetic context by constructing a maximum-likelihood tree. Two samples were positive and could be identified as Babesia species. Through phylogenetic analyses, both sequences were demonstrated to belong to a group of Babesia sp. associated with marsupials from Brazil and therefore previously defined as the “South American Marsupialia Group” (SAMG) of Babesia species. The results of this study represent the first report of a Piroplasmida species in a marsupial in Argentina. They further give an insight on the circulation of Babesia sp. in D. albiventris that, due to its synanthropic habits, plays an epidemiologically important role as potential hosts for emerging pathogens and ectoparasites of relevance in veterinary medicine.
publishDate 2025
dc.date.none.fl_str_mv 2025-02-20T11:39:42Z
2025-02-20T11:39:42Z
2025-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/21367
https://www.sciencedirect.com/science/article/abs/pii/S2405939025000127
2405-9390
https://doi.org/10.1016/j.vprsr.2025.101205
url http://hdl.handle.net/20.500.12123/21367
https://www.sciencedirect.com/science/article/abs/pii/S2405939025000127
https://doi.org/10.1016/j.vprsr.2025.101205
identifier_str_mv 2405-9390
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PE-E5-I109-001, Convocatoria: Estudios para el control de enfermedades subtropicales y/o transmitidas por vectores (Tristeza Bovina, Garrapatas, Miasis, Tripanosomiasis, Lengua Azul y la
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Parasitology: Regional Studies and Reports 58 : 101205. (February 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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