Measurement of malondialdehyde as oxidative stress biomarker in goat plasma by HPLC-DAD

Autores
Yonny, Melisa Evangelina; García, Elisa Mariana; Lopez, Agustin; Arroquy, Jose Ignacio; Nazareno, Mónica Azucena
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
During the cattle breeding, the animals are exposed to stressful conditions (confinement, competition, extreme temperatures, pathogens); these have been associated to the increase of reactive oxygen species that attack cell membrane unsaturated lipids and other biomolecules and thus, inducing oxidation. This oxidative stress increases the incidence of degenerative diseases and decreases productivity. It has been proposed that the supplementation of the animal diet with antioxidants decreases the oxidative stress. Malondialdehyde (MDA) is an advanced oxidation product and a recognized oxidative stress biomarker. The aims of this work were to validate a methodology to determine MDA in goat plasma by HPLC-DAD and its assay in an animal feeding experiment. The MDA level was measured after condensation reaction with thiobarbituric acid (TBA). Free MDA (FMDA) was directly quantified after TBA condensation; while, Protein-bound MDA (PBMDA) needed to be released by an alkaline hydrolysis step and followed by TBA condensation. The MDA detection and quantitation limits were 0.034 μM and 0.086 μM, respectively. The linear range was 0.086–9.1 μM (R2 = 0.9998). The accuracy was 100.5% to FMDA and 102.4% to PBMDA. The precision values intra and interday were 3.7 and 3.9%, respectively, for FMDA; and 3.8 and 4.1%, respectively, for PBMDA. Samples were analyzed in triplicate to obtain the figures of merit. The MDA level was quantified in plasma samples of 19 goats (Capra hircus) randomly distributed in three groups and fed with isocaloric and isoproteic diets: control, and supplemented with 12.5% d.b. of native woody species as polyphenol sources. The MDA plasma levels were statistically different among treatments in regard to the animal diet. A protector effect of the supplementation against oxidative stress was observed. The proposed methodology was selective, sensitive, reproducible, and suitable to evaluate MDA in goat plasma as oxidative stress biomarker.
EEA Santiago del Estero
Fil: Yonny, Melisa Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: García, Elisa Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: López, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina
Fil: Arroquy, Jose Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina
Fil: Nazareno, Mónica Azucena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fuente
Microchemical journal 129 : 281-285. (November 2016)
Materia
Caprinos
Plasma Sanguíneo
Malondialdehído
Estrés Oxidativo
Goats
Blood Plasma
Malondialdehyde
Oxidative Stress
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2558
Acceder
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oai_identifier_str oai:localhost:20.500.12123/2558
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Measurement of malondialdehyde as oxidative stress biomarker in goat plasma by HPLC-DADYonny, Melisa EvangelinaGarcía, Elisa MarianaLopez, AgustinArroquy, Jose IgnacioNazareno, Mónica AzucenaCaprinosPlasma SanguíneoMalondialdehídoEstrés OxidativoGoatsBlood PlasmaMalondialdehydeOxidative StressDuring the cattle breeding, the animals are exposed to stressful conditions (confinement, competition, extreme temperatures, pathogens); these have been associated to the increase of reactive oxygen species that attack cell membrane unsaturated lipids and other biomolecules and thus, inducing oxidation. This oxidative stress increases the incidence of degenerative diseases and decreases productivity. It has been proposed that the supplementation of the animal diet with antioxidants decreases the oxidative stress. Malondialdehyde (MDA) is an advanced oxidation product and a recognized oxidative stress biomarker. The aims of this work were to validate a methodology to determine MDA in goat plasma by HPLC-DAD and its assay in an animal feeding experiment. The MDA level was measured after condensation reaction with thiobarbituric acid (TBA). Free MDA (FMDA) was directly quantified after TBA condensation; while, Protein-bound MDA (PBMDA) needed to be released by an alkaline hydrolysis step and followed by TBA condensation. The MDA detection and quantitation limits were 0.034 μM and 0.086 μM, respectively. The linear range was 0.086–9.1 μM (R2 = 0.9998). The accuracy was 100.5% to FMDA and 102.4% to PBMDA. The precision values intra and interday were 3.7 and 3.9%, respectively, for FMDA; and 3.8 and 4.1%, respectively, for PBMDA. Samples were analyzed in triplicate to obtain the figures of merit. The MDA level was quantified in plasma samples of 19 goats (Capra hircus) randomly distributed in three groups and fed with isocaloric and isoproteic diets: control, and supplemented with 12.5% d.b. of native woody species as polyphenol sources. The MDA plasma levels were statistically different among treatments in regard to the animal diet. A protector effect of the supplementation against oxidative stress was observed. The proposed methodology was selective, sensitive, reproducible, and suitable to evaluate MDA in goat plasma as oxidative stress biomarker.EEA Santiago del EsteroFil: Yonny, Melisa Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaFil: García, Elisa Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; ArgentinaFil: López, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; ArgentinaFil: Arroquy, Jose Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; ArgentinaFil: Nazareno, Mónica Azucena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina2018-06-06T14:10:43Z2018-06-06T14:10:43Z2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.sciencedirect.com/science/article/pii/S0026265X16301734http://hdl.handle.net/20.500.12123/25580026-265Xhttps://doi.org/10.1016/j.microc.2016.07.010Microchemical journal 129 : 281-285. (November 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:20Zoai:localhost:20.500.12123/2558instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:20.502INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Measurement of malondialdehyde as oxidative stress biomarker in goat plasma by HPLC-DAD
title Measurement of malondialdehyde as oxidative stress biomarker in goat plasma by HPLC-DAD
spellingShingle Measurement of malondialdehyde as oxidative stress biomarker in goat plasma by HPLC-DAD
Yonny, Melisa Evangelina
Caprinos
Plasma Sanguíneo
Malondialdehído
Estrés Oxidativo
Goats
Blood Plasma
Malondialdehyde
Oxidative Stress
title_short Measurement of malondialdehyde as oxidative stress biomarker in goat plasma by HPLC-DAD
title_full Measurement of malondialdehyde as oxidative stress biomarker in goat plasma by HPLC-DAD
title_fullStr Measurement of malondialdehyde as oxidative stress biomarker in goat plasma by HPLC-DAD
title_full_unstemmed Measurement of malondialdehyde as oxidative stress biomarker in goat plasma by HPLC-DAD
title_sort Measurement of malondialdehyde as oxidative stress biomarker in goat plasma by HPLC-DAD
dc.creator.none.fl_str_mv Yonny, Melisa Evangelina
García, Elisa Mariana
Lopez, Agustin
Arroquy, Jose Ignacio
Nazareno, Mónica Azucena
author Yonny, Melisa Evangelina
author_facet Yonny, Melisa Evangelina
García, Elisa Mariana
Lopez, Agustin
Arroquy, Jose Ignacio
Nazareno, Mónica Azucena
author_role author
author2 García, Elisa Mariana
Lopez, Agustin
Arroquy, Jose Ignacio
Nazareno, Mónica Azucena
author2_role author
author
author
author
dc.subject.none.fl_str_mv Caprinos
Plasma Sanguíneo
Malondialdehído
Estrés Oxidativo
Goats
Blood Plasma
Malondialdehyde
Oxidative Stress
topic Caprinos
Plasma Sanguíneo
Malondialdehído
Estrés Oxidativo
Goats
Blood Plasma
Malondialdehyde
Oxidative Stress
dc.description.none.fl_txt_mv During the cattle breeding, the animals are exposed to stressful conditions (confinement, competition, extreme temperatures, pathogens); these have been associated to the increase of reactive oxygen species that attack cell membrane unsaturated lipids and other biomolecules and thus, inducing oxidation. This oxidative stress increases the incidence of degenerative diseases and decreases productivity. It has been proposed that the supplementation of the animal diet with antioxidants decreases the oxidative stress. Malondialdehyde (MDA) is an advanced oxidation product and a recognized oxidative stress biomarker. The aims of this work were to validate a methodology to determine MDA in goat plasma by HPLC-DAD and its assay in an animal feeding experiment. The MDA level was measured after condensation reaction with thiobarbituric acid (TBA). Free MDA (FMDA) was directly quantified after TBA condensation; while, Protein-bound MDA (PBMDA) needed to be released by an alkaline hydrolysis step and followed by TBA condensation. The MDA detection and quantitation limits were 0.034 μM and 0.086 μM, respectively. The linear range was 0.086–9.1 μM (R2 = 0.9998). The accuracy was 100.5% to FMDA and 102.4% to PBMDA. The precision values intra and interday were 3.7 and 3.9%, respectively, for FMDA; and 3.8 and 4.1%, respectively, for PBMDA. Samples were analyzed in triplicate to obtain the figures of merit. The MDA level was quantified in plasma samples of 19 goats (Capra hircus) randomly distributed in three groups and fed with isocaloric and isoproteic diets: control, and supplemented with 12.5% d.b. of native woody species as polyphenol sources. The MDA plasma levels were statistically different among treatments in regard to the animal diet. A protector effect of the supplementation against oxidative stress was observed. The proposed methodology was selective, sensitive, reproducible, and suitable to evaluate MDA in goat plasma as oxidative stress biomarker.
EEA Santiago del Estero
Fil: Yonny, Melisa Evangelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: García, Elisa Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
Fil: López, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina
Fil: Arroquy, Jose Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina
Fil: Nazareno, Mónica Azucena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones y Transferencia de Santiago del Estero. Universidad Nacional de Santiago del Estero. Centro de Investigaciones y Transferencia de Santiago del Estero; Argentina
description During the cattle breeding, the animals are exposed to stressful conditions (confinement, competition, extreme temperatures, pathogens); these have been associated to the increase of reactive oxygen species that attack cell membrane unsaturated lipids and other biomolecules and thus, inducing oxidation. This oxidative stress increases the incidence of degenerative diseases and decreases productivity. It has been proposed that the supplementation of the animal diet with antioxidants decreases the oxidative stress. Malondialdehyde (MDA) is an advanced oxidation product and a recognized oxidative stress biomarker. The aims of this work were to validate a methodology to determine MDA in goat plasma by HPLC-DAD and its assay in an animal feeding experiment. The MDA level was measured after condensation reaction with thiobarbituric acid (TBA). Free MDA (FMDA) was directly quantified after TBA condensation; while, Protein-bound MDA (PBMDA) needed to be released by an alkaline hydrolysis step and followed by TBA condensation. The MDA detection and quantitation limits were 0.034 μM and 0.086 μM, respectively. The linear range was 0.086–9.1 μM (R2 = 0.9998). The accuracy was 100.5% to FMDA and 102.4% to PBMDA. The precision values intra and interday were 3.7 and 3.9%, respectively, for FMDA; and 3.8 and 4.1%, respectively, for PBMDA. Samples were analyzed in triplicate to obtain the figures of merit. The MDA level was quantified in plasma samples of 19 goats (Capra hircus) randomly distributed in three groups and fed with isocaloric and isoproteic diets: control, and supplemented with 12.5% d.b. of native woody species as polyphenol sources. The MDA plasma levels were statistically different among treatments in regard to the animal diet. A protector effect of the supplementation against oxidative stress was observed. The proposed methodology was selective, sensitive, reproducible, and suitable to evaluate MDA in goat plasma as oxidative stress biomarker.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
2018-06-06T14:10:43Z
2018-06-06T14:10:43Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.sciencedirect.com/science/article/pii/S0026265X16301734
http://hdl.handle.net/20.500.12123/2558
0026-265X
https://doi.org/10.1016/j.microc.2016.07.010
url https://www.sciencedirect.com/science/article/pii/S0026265X16301734
http://hdl.handle.net/20.500.12123/2558
https://doi.org/10.1016/j.microc.2016.07.010
identifier_str_mv 0026-265X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Microchemical journal 129 : 281-285. (November 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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