Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii

Autores
Pavanelo, Daniel Brisotto; Schröder, Nicolas C.H.; Pin Viso, Natalia Daniela; Martins, Larissa A.; Malossi, Camila Dantas; Galletti, Maria Fernanda B.M.; Labruna, Marcelo B.; Daffre, Sirlei; Farber, Marisa Diana; Fogaça, Andréa C.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although the ticks Amblyomma sculptum and Amblyomma aureolatum are important vectors of Rickettsia rickettsii, causative agent of the life-threatening Rocky Mountain spotted fever, A. aureolatum is considerably more susceptible to infection than A. sculptum. As the microbiota can interfere with the colonization of arthropod midgut (MG) by pathogens, in the current study we analyzed the MG microbiota of both tick species. Our results revealed that the MG of A. aureolatum harbors a prominent microbiota, while A. sculptum does not. Remarkably, a significant reduction of the bacterial load was recorded in R. rickettsii-infected A. aureolatum. In addition, the taxonomy analysis of the MG bacterial community of A. aureolatum revealed a dominance of the genus Francisella, suggesting an endosymbiosis. This study is the first step in getting insights into the mechanisms underlying the interactions among Amblyomma species, their microbiota and R. rickettsii. Additional studies to better understand these mechanisms are required and may help the development of novel alternatives to block rickettsial transmission.
Instituto de Biotecnología
Fil: Pavanelo, Daniel Brisotto. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil
Fil: Schröder, Nicolas C.H. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil
Fil: Pin Viso, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Martins, Larissa A. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil
Fil: Malossi, Camila Dantas. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil
Fil: Galletti, Maria Fernanda B.M. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil
Fil: Labruna, Marcelo B. University of São Paulo. Faculty of Veterinary Medicine and Husbandry. Department of Preventive Veterinary Medicine and Animal Health; Brasil
Fil: Daffre, Sirlei. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil
Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Fogaça, Andréa C. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil
Fuente
Developmental & Comparative Immunology 106 : 103606 (May 2020)
Materia
Rickettsia
Vectores
Relaciones Huésped Parásito
Flora Microbiana
Vectors
Host Parasite Relations
Microbial Flora
Garrapatas
Rickettsia rickettsii
Microbiota
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7745
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7745
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spelling Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsiiPavanelo, Daniel BrisottoSchröder, Nicolas C.H.Pin Viso, Natalia DanielaMartins, Larissa A.Malossi, Camila DantasGalletti, Maria Fernanda B.M.Labruna, Marcelo B.Daffre, SirleiFarber, Marisa DianaFogaça, Andréa C.RickettsiaVectoresRelaciones Huésped ParásitoFlora MicrobianaVectorsHost Parasite RelationsMicrobial FloraGarrapatasRickettsia rickettsiiMicrobiotaAlthough the ticks Amblyomma sculptum and Amblyomma aureolatum are important vectors of Rickettsia rickettsii, causative agent of the life-threatening Rocky Mountain spotted fever, A. aureolatum is considerably more susceptible to infection than A. sculptum. As the microbiota can interfere with the colonization of arthropod midgut (MG) by pathogens, in the current study we analyzed the MG microbiota of both tick species. Our results revealed that the MG of A. aureolatum harbors a prominent microbiota, while A. sculptum does not. Remarkably, a significant reduction of the bacterial load was recorded in R. rickettsii-infected A. aureolatum. In addition, the taxonomy analysis of the MG bacterial community of A. aureolatum revealed a dominance of the genus Francisella, suggesting an endosymbiosis. This study is the first step in getting insights into the mechanisms underlying the interactions among Amblyomma species, their microbiota and R. rickettsii. Additional studies to better understand these mechanisms are required and may help the development of novel alternatives to block rickettsial transmission.Instituto de BiotecnologíaFil: Pavanelo, Daniel Brisotto. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; BrasilFil: Schröder, Nicolas C.H. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; BrasilFil: Pin Viso, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Martins, Larissa A. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; BrasilFil: Malossi, Camila Dantas. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; BrasilFil: Galletti, Maria Fernanda B.M. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; BrasilFil: Labruna, Marcelo B. University of São Paulo. Faculty of Veterinary Medicine and Husbandry. Department of Preventive Veterinary Medicine and Animal Health; BrasilFil: Daffre, Sirlei. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; BrasilFil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Fogaça, Andréa C. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; BrasilElsevier2020-08-20T13:17:47Z2020-08-20T13:17:47Z2020-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7745https://www.sciencedirect.com/science/article/pii/S0145305X193051780145-305Xhttps://doi.org/10.1016/j.dci.2019.103606Developmental & Comparative Immunology 106 : 103606 (May 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-11-27T08:38:35Zoai:localhost:20.500.12123/7745instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-27 08:38:35.488INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii
title Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii
spellingShingle Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii
Pavanelo, Daniel Brisotto
Rickettsia
Vectores
Relaciones Huésped Parásito
Flora Microbiana
Vectors
Host Parasite Relations
Microbial Flora
Garrapatas
Rickettsia rickettsii
Microbiota
title_short Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii
title_full Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii
title_fullStr Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii
title_full_unstemmed Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii
title_sort Comparative analysis of the midgut microbiota of two natural tick vectors of Rickettsia rickettsii
dc.creator.none.fl_str_mv Pavanelo, Daniel Brisotto
Schröder, Nicolas C.H.
Pin Viso, Natalia Daniela
Martins, Larissa A.
Malossi, Camila Dantas
Galletti, Maria Fernanda B.M.
Labruna, Marcelo B.
Daffre, Sirlei
Farber, Marisa Diana
Fogaça, Andréa C.
author Pavanelo, Daniel Brisotto
author_facet Pavanelo, Daniel Brisotto
Schröder, Nicolas C.H.
Pin Viso, Natalia Daniela
Martins, Larissa A.
Malossi, Camila Dantas
Galletti, Maria Fernanda B.M.
Labruna, Marcelo B.
Daffre, Sirlei
Farber, Marisa Diana
Fogaça, Andréa C.
author_role author
author2 Schröder, Nicolas C.H.
Pin Viso, Natalia Daniela
Martins, Larissa A.
Malossi, Camila Dantas
Galletti, Maria Fernanda B.M.
Labruna, Marcelo B.
Daffre, Sirlei
Farber, Marisa Diana
Fogaça, Andréa C.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Rickettsia
Vectores
Relaciones Huésped Parásito
Flora Microbiana
Vectors
Host Parasite Relations
Microbial Flora
Garrapatas
Rickettsia rickettsii
Microbiota
topic Rickettsia
Vectores
Relaciones Huésped Parásito
Flora Microbiana
Vectors
Host Parasite Relations
Microbial Flora
Garrapatas
Rickettsia rickettsii
Microbiota
dc.description.none.fl_txt_mv Although the ticks Amblyomma sculptum and Amblyomma aureolatum are important vectors of Rickettsia rickettsii, causative agent of the life-threatening Rocky Mountain spotted fever, A. aureolatum is considerably more susceptible to infection than A. sculptum. As the microbiota can interfere with the colonization of arthropod midgut (MG) by pathogens, in the current study we analyzed the MG microbiota of both tick species. Our results revealed that the MG of A. aureolatum harbors a prominent microbiota, while A. sculptum does not. Remarkably, a significant reduction of the bacterial load was recorded in R. rickettsii-infected A. aureolatum. In addition, the taxonomy analysis of the MG bacterial community of A. aureolatum revealed a dominance of the genus Francisella, suggesting an endosymbiosis. This study is the first step in getting insights into the mechanisms underlying the interactions among Amblyomma species, their microbiota and R. rickettsii. Additional studies to better understand these mechanisms are required and may help the development of novel alternatives to block rickettsial transmission.
Instituto de Biotecnología
Fil: Pavanelo, Daniel Brisotto. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil
Fil: Schröder, Nicolas C.H. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil
Fil: Pin Viso, Natalia Daniela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Martins, Larissa A. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil
Fil: Malossi, Camila Dantas. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil
Fil: Galletti, Maria Fernanda B.M. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil
Fil: Labruna, Marcelo B. University of São Paulo. Faculty of Veterinary Medicine and Husbandry. Department of Preventive Veterinary Medicine and Animal Health; Brasil
Fil: Daffre, Sirlei. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil
Fil: Farber, Marisa Diana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Fogaça, Andréa C. University of São Paulo. Institute of Biomedical Sciences. Department of Parasitology; Brasil
description Although the ticks Amblyomma sculptum and Amblyomma aureolatum are important vectors of Rickettsia rickettsii, causative agent of the life-threatening Rocky Mountain spotted fever, A. aureolatum is considerably more susceptible to infection than A. sculptum. As the microbiota can interfere with the colonization of arthropod midgut (MG) by pathogens, in the current study we analyzed the MG microbiota of both tick species. Our results revealed that the MG of A. aureolatum harbors a prominent microbiota, while A. sculptum does not. Remarkably, a significant reduction of the bacterial load was recorded in R. rickettsii-infected A. aureolatum. In addition, the taxonomy analysis of the MG bacterial community of A. aureolatum revealed a dominance of the genus Francisella, suggesting an endosymbiosis. This study is the first step in getting insights into the mechanisms underlying the interactions among Amblyomma species, their microbiota and R. rickettsii. Additional studies to better understand these mechanisms are required and may help the development of novel alternatives to block rickettsial transmission.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-20T13:17:47Z
2020-08-20T13:17:47Z
2020-05
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7745
https://www.sciencedirect.com/science/article/pii/S0145305X19305178
0145-305X
https://doi.org/10.1016/j.dci.2019.103606
url http://hdl.handle.net/20.500.12123/7745
https://www.sciencedirect.com/science/article/pii/S0145305X19305178
https://doi.org/10.1016/j.dci.2019.103606
identifier_str_mv 0145-305X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Developmental & Comparative Immunology 106 : 103606 (May 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 13.011256

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