Whole Cottonseed as an Alternative to Mitigate In Vitro Methane Emissions on Low-Quality Forage-Based Diets

Autores
Hernandez, Olegario; Juárez Sequeira, Ana Verónica; García, Elisa Mariana; Zarate García, Natalia; Uñates Pellene, Francisco Augusto; Fissolo, Hector Miguel; Coria, María Sumampa; Lopez, Agustin
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Enteric methane (CH4) emissions from ruminants are one of the major contributors to greenhouse gas emissions (GHG) from agriculture. We hypothesized that increasing levels of whole cottonseed (WCS) in diets decreases CH4 production in ruminants fed low-quality forages without compromising feed digestibility. Dietary lipid supplementation, particularly with oilseeds such as WCS, has the potential to reduce enteric CH4 production by altering rumen fermentation pathways. This study aimed to evaluate the effects of WCS inclusion in a tropical forage-based diet on in vitro gas and CH4 production, fermentation characteristics, and in vitro dry matter digestibility (IVDMD). Five treatments were tested using guinea grass hay (GG) supplemented with 0, 12.5, 25, 37.5, and 50% of WCS (DM basis). Results after 48 h of incubation showed a quadratic response of total gas production with increasing WCS inclusion (p < 0.01), with the highest values observed at 12.5% and 25% WCS. Methane production from digested DM significantly reduced at 50% WCS inclusion (−10.66% respect to control; p = 0.02), suggesting a mitigation effect without detrimental impacts on IVDMD (p = 0.16), which remained unaffected across all treatments. No effects were found in initial or final pH (p = 0.98 and p = 0.89, respectively) or total protozoa count among treatments (p = 0.99). However, levels of 50% WCS inclusion exceed the recommended limit of fat in ruminant diets (>6% DM). Further in vivo validation trials are recommended to confirm these in vitro results and evaluate long-term impacts on animal performance and CH4 emissions. This article is a revised and expanded version of Whole cottonseed as an alternative to mitigate in vitro methane emissions on low-quality forage-based diets. Presented at the International Research Symposium on Agricultural Greenhouse Gas Mitigation. From Research to Implementation. 21–24 October 2024, Berlin, Germany.
EEA Santiago del Estero
Fil: Hernandez, Olegario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina
Fil: Hernandez, Olegario. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina
Fil: Juárez Sequeira, A.V. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.
Fil: Juárez Sequeira, A.V. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Bionanotecnología del NOA; Argentina
Fil: Juárez Sequeira, A.V. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del NOA; Argentina
Fil: García, Elisa Mariana. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina
Fil: García, Elisa Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García, Elisa Mariana. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Instituto de Ciencias Químicas. Laboratorio de Antioxidantes y Procesos Oxidativos; Argentina
Fil: Zarate García, Natalia. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina
Fil: Uñates Pellene, Francisco Augusto. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina
Fil: Fissolo, Héctor Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina
Fil: Coria, María Sumampa. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.
Fil: Coria, María Sumampa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Bionanotecnología del NOA; Argentina
Fil: Coria, María Sumampa. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del NOA; Argentina
Fil: Coria, María Sumampa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: López, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina.
Fil: López, Agustín. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.
Fil: López, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Environments 12 (12) : 459. (December 2025)
Materia
Semilla de Algodón
Experimentación In Vitro
Emisión de Metano
Forrajes
Gases de Efecto Invernadero
Cottonseed
In Vitro Experimentation
Methane Emission
Forage
Greenhouse Gas Emissions
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24857
Acceder
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oai_identifier_str oai:localhost:20.500.12123/24857
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spelling Whole Cottonseed as an Alternative to Mitigate In Vitro Methane Emissions on Low-Quality Forage-Based DietsHernandez, OlegarioJuárez Sequeira, Ana VerónicaGarcía, Elisa MarianaZarate García, NataliaUñates Pellene, Francisco AugustoFissolo, Hector MiguelCoria, María SumampaLopez, AgustinSemilla de AlgodónExperimentación In VitroEmisión de MetanoForrajesGases de Efecto InvernaderoCottonseedIn Vitro ExperimentationMethane EmissionForageGreenhouse Gas EmissionsEnteric methane (CH4) emissions from ruminants are one of the major contributors to greenhouse gas emissions (GHG) from agriculture. We hypothesized that increasing levels of whole cottonseed (WCS) in diets decreases CH4 production in ruminants fed low-quality forages without compromising feed digestibility. Dietary lipid supplementation, particularly with oilseeds such as WCS, has the potential to reduce enteric CH4 production by altering rumen fermentation pathways. This study aimed to evaluate the effects of WCS inclusion in a tropical forage-based diet on in vitro gas and CH4 production, fermentation characteristics, and in vitro dry matter digestibility (IVDMD). Five treatments were tested using guinea grass hay (GG) supplemented with 0, 12.5, 25, 37.5, and 50% of WCS (DM basis). Results after 48 h of incubation showed a quadratic response of total gas production with increasing WCS inclusion (p < 0.01), with the highest values observed at 12.5% and 25% WCS. Methane production from digested DM significantly reduced at 50% WCS inclusion (−10.66% respect to control; p = 0.02), suggesting a mitigation effect without detrimental impacts on IVDMD (p = 0.16), which remained unaffected across all treatments. No effects were found in initial or final pH (p = 0.98 and p = 0.89, respectively) or total protozoa count among treatments (p = 0.99). However, levels of 50% WCS inclusion exceed the recommended limit of fat in ruminant diets (>6% DM). Further in vivo validation trials are recommended to confirm these in vitro results and evaluate long-term impacts on animal performance and CH4 emissions. This article is a revised and expanded version of Whole cottonseed as an alternative to mitigate in vitro methane emissions on low-quality forage-based diets. Presented at the International Research Symposium on Agricultural Greenhouse Gas Mitigation. From Research to Implementation. 21–24 October 2024, Berlin, Germany.EEA Santiago del EsteroFil: Hernandez, Olegario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; ArgentinaFil: Hernandez, Olegario. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: Juárez Sequeira, A.V. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.Fil: Juárez Sequeira, A.V. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Bionanotecnología del NOA; ArgentinaFil: Juárez Sequeira, A.V. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del NOA; ArgentinaFil: García, Elisa Mariana. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: García, Elisa Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: García, Elisa Mariana. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Instituto de Ciencias Químicas. Laboratorio de Antioxidantes y Procesos Oxidativos; ArgentinaFil: Zarate García, Natalia. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: Uñates Pellene, Francisco Augusto. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; ArgentinaFil: Fissolo, Héctor Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; ArgentinaFil: Coria, María Sumampa. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.Fil: Coria, María Sumampa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Bionanotecnología del NOA; ArgentinaFil: Coria, María Sumampa. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del NOA; ArgentinaFil: Coria, María Sumampa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: López, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina.Fil: López, Agustín. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.Fil: López, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaMDPI2026-01-02T15:12:16Z2026-01-02T15:12:16Z2025-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24857https://www.mdpi.com/2076-3298/12/12/4592076-3298https://doi.org/10.3390/environments12120459Environments 12 (12) : 459. (December 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2026-01-08T10:41:02Zoai:localhost:20.500.12123/24857instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2026-01-08 10:41:02.805INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Whole Cottonseed as an Alternative to Mitigate In Vitro Methane Emissions on Low-Quality Forage-Based Diets
title Whole Cottonseed as an Alternative to Mitigate In Vitro Methane Emissions on Low-Quality Forage-Based Diets
spellingShingle Whole Cottonseed as an Alternative to Mitigate In Vitro Methane Emissions on Low-Quality Forage-Based Diets
Hernandez, Olegario
Semilla de Algodón
Experimentación In Vitro
Emisión de Metano
Forrajes
Gases de Efecto Invernadero
Cottonseed
In Vitro Experimentation
Methane Emission
Forage
Greenhouse Gas Emissions
title_short Whole Cottonseed as an Alternative to Mitigate In Vitro Methane Emissions on Low-Quality Forage-Based Diets
title_full Whole Cottonseed as an Alternative to Mitigate In Vitro Methane Emissions on Low-Quality Forage-Based Diets
title_fullStr Whole Cottonseed as an Alternative to Mitigate In Vitro Methane Emissions on Low-Quality Forage-Based Diets
title_full_unstemmed Whole Cottonseed as an Alternative to Mitigate In Vitro Methane Emissions on Low-Quality Forage-Based Diets
title_sort Whole Cottonseed as an Alternative to Mitigate In Vitro Methane Emissions on Low-Quality Forage-Based Diets
dc.creator.none.fl_str_mv Hernandez, Olegario
Juárez Sequeira, Ana Verónica
García, Elisa Mariana
Zarate García, Natalia
Uñates Pellene, Francisco Augusto
Fissolo, Hector Miguel
Coria, María Sumampa
Lopez, Agustin
author Hernandez, Olegario
author_facet Hernandez, Olegario
Juárez Sequeira, Ana Verónica
García, Elisa Mariana
Zarate García, Natalia
Uñates Pellene, Francisco Augusto
Fissolo, Hector Miguel
Coria, María Sumampa
Lopez, Agustin
author_role author
author2 Juárez Sequeira, Ana Verónica
García, Elisa Mariana
Zarate García, Natalia
Uñates Pellene, Francisco Augusto
Fissolo, Hector Miguel
Coria, María Sumampa
Lopez, Agustin
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Semilla de Algodón
Experimentación In Vitro
Emisión de Metano
Forrajes
Gases de Efecto Invernadero
Cottonseed
In Vitro Experimentation
Methane Emission
Forage
Greenhouse Gas Emissions
topic Semilla de Algodón
Experimentación In Vitro
Emisión de Metano
Forrajes
Gases de Efecto Invernadero
Cottonseed
In Vitro Experimentation
Methane Emission
Forage
Greenhouse Gas Emissions
dc.description.none.fl_txt_mv Enteric methane (CH4) emissions from ruminants are one of the major contributors to greenhouse gas emissions (GHG) from agriculture. We hypothesized that increasing levels of whole cottonseed (WCS) in diets decreases CH4 production in ruminants fed low-quality forages without compromising feed digestibility. Dietary lipid supplementation, particularly with oilseeds such as WCS, has the potential to reduce enteric CH4 production by altering rumen fermentation pathways. This study aimed to evaluate the effects of WCS inclusion in a tropical forage-based diet on in vitro gas and CH4 production, fermentation characteristics, and in vitro dry matter digestibility (IVDMD). Five treatments were tested using guinea grass hay (GG) supplemented with 0, 12.5, 25, 37.5, and 50% of WCS (DM basis). Results after 48 h of incubation showed a quadratic response of total gas production with increasing WCS inclusion (p < 0.01), with the highest values observed at 12.5% and 25% WCS. Methane production from digested DM significantly reduced at 50% WCS inclusion (−10.66% respect to control; p = 0.02), suggesting a mitigation effect without detrimental impacts on IVDMD (p = 0.16), which remained unaffected across all treatments. No effects were found in initial or final pH (p = 0.98 and p = 0.89, respectively) or total protozoa count among treatments (p = 0.99). However, levels of 50% WCS inclusion exceed the recommended limit of fat in ruminant diets (>6% DM). Further in vivo validation trials are recommended to confirm these in vitro results and evaluate long-term impacts on animal performance and CH4 emissions. This article is a revised and expanded version of Whole cottonseed as an alternative to mitigate in vitro methane emissions on low-quality forage-based diets. Presented at the International Research Symposium on Agricultural Greenhouse Gas Mitigation. From Research to Implementation. 21–24 October 2024, Berlin, Germany.
EEA Santiago del Estero
Fil: Hernandez, Olegario. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina
Fil: Hernandez, Olegario. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina
Fil: Juárez Sequeira, A.V. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.
Fil: Juárez Sequeira, A.V. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Bionanotecnología del NOA; Argentina
Fil: Juárez Sequeira, A.V. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del NOA; Argentina
Fil: García, Elisa Mariana. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina
Fil: García, Elisa Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: García, Elisa Mariana. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias. Instituto de Ciencias Químicas. Laboratorio de Antioxidantes y Procesos Oxidativos; Argentina
Fil: Zarate García, Natalia. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina
Fil: Uñates Pellene, Francisco Augusto. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina
Fil: Fissolo, Héctor Miguel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina
Fil: Coria, María Sumampa. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.
Fil: Coria, María Sumampa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Bionanotecnología del NOA; Argentina
Fil: Coria, María Sumampa. Universidad Nacional de Santiago del Estero. Instituto de Bionanotecnología del NOA; Argentina
Fil: Coria, María Sumampa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: López, Agustín. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Santiago del Estero; Argentina.
Fil: López, Agustín. Universidad Nacional de Santiago del Estero. Facultad de Agronomía y Agroindustrias; Argentina.
Fil: López, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Enteric methane (CH4) emissions from ruminants are one of the major contributors to greenhouse gas emissions (GHG) from agriculture. We hypothesized that increasing levels of whole cottonseed (WCS) in diets decreases CH4 production in ruminants fed low-quality forages without compromising feed digestibility. Dietary lipid supplementation, particularly with oilseeds such as WCS, has the potential to reduce enteric CH4 production by altering rumen fermentation pathways. This study aimed to evaluate the effects of WCS inclusion in a tropical forage-based diet on in vitro gas and CH4 production, fermentation characteristics, and in vitro dry matter digestibility (IVDMD). Five treatments were tested using guinea grass hay (GG) supplemented with 0, 12.5, 25, 37.5, and 50% of WCS (DM basis). Results after 48 h of incubation showed a quadratic response of total gas production with increasing WCS inclusion (p < 0.01), with the highest values observed at 12.5% and 25% WCS. Methane production from digested DM significantly reduced at 50% WCS inclusion (−10.66% respect to control; p = 0.02), suggesting a mitigation effect without detrimental impacts on IVDMD (p = 0.16), which remained unaffected across all treatments. No effects were found in initial or final pH (p = 0.98 and p = 0.89, respectively) or total protozoa count among treatments (p = 0.99). However, levels of 50% WCS inclusion exceed the recommended limit of fat in ruminant diets (>6% DM). Further in vivo validation trials are recommended to confirm these in vitro results and evaluate long-term impacts on animal performance and CH4 emissions. This article is a revised and expanded version of Whole cottonseed as an alternative to mitigate in vitro methane emissions on low-quality forage-based diets. Presented at the International Research Symposium on Agricultural Greenhouse Gas Mitigation. From Research to Implementation. 21–24 October 2024, Berlin, Germany.
publishDate 2025
dc.date.none.fl_str_mv 2025-12
2026-01-02T15:12:16Z
2026-01-02T15:12:16Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24857
https://www.mdpi.com/2076-3298/12/12/459
2076-3298
https://doi.org/10.3390/environments12120459
url http://hdl.handle.net/20.500.12123/24857
https://www.mdpi.com/2076-3298/12/12/459
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Environments 12 (12) : 459. (December 2025)
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reponame_str INTA Digital (INTA)
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