Negative modulation of SA signaling components by the capsid protein of tobacco mosaic virus is required for viral long-distance movement

Autores
Venturuzzi, Andrea Laura; Rodriguez, Maria Cecilia; Conti, Gabriela; Leone, Melisa; Caro, Maria Del Pilar; Montecchia, Juan Francisco; Zavallo, Diego; Asurmendi, Sebastian
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
An important aspect of plant–virus interaction is the way viruses dynamically move over long distances and how plant immunity modulates viral systemic movement. Salicylic acid (SA), a well-characterized hormone responsible for immune responses against virus, is activated through different transcription factors including TGA and WRKY. In tobamoviruses, evidence suggests that capsid protein (CP) is required for long-distance movement, although its precise role has not been fully characterized yet. Previously, we showed that the CP of Tobacco Mosaic Virus (TMV)-Cg negatively modulates the SA-mediated defense. In this study, we analyzed the impact of SA-defense mechanism on the long-distance transport of a truncated version of TMV (TMV ∆CP virus) that cannot move to systemic tissues. The study showed that the negative modulation of NPR1 and TGA10 factors allows the long-distance transport of TMV ∆CP virus. Moreover, we observed that the stabilization of DELLA proteins promotes TMV ∆CP systemic movement. We also characterized a group of genes, part of a network modulated by CP, involved in TMV ∆CP long-distance transport. Altogether, our results indicate that CP-mediated downregulation of SA signaling pathway is required for the virus systemic movement, and this role of CP may be linked to its ability to stabilize DELLA proteins.
Instituto de Biotecnología
Fil: Venturuzzi, Andrea Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Venturuzzi, Andrea Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rodriguez, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Conti, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leone, Melisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Leone, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caro, María del Pilar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Caro, María del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Montecchia, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Montecchia, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Zavallo, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
The Plant Journal 106 (4) : 896–912 (May 2021)
Materia
Coat Poteins
Tobamovirus
Plant Viruses
Tobacco Mosaic Tobamovirus
Salicylic Acids
Immune Response
Proteínas de la Cubierta
Virus de las Plantas
Tobamovirus del Mosaico del Tabaco
Ácido Salicílico
Respuesta Inmunológica
Systemic Movement
DELLA Proteins
Movimiento Sistémico
Proteínas DELLA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/18792
Acceder
id INTADig_9522e3d4eb06240ccca69bd9d6e9fc3e
oai_identifier_str oai:localhost:20.500.12123/18792
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Negative modulation of SA signaling components by the capsid protein of tobacco mosaic virus is required for viral long-distance movementVenturuzzi, Andrea LauraRodriguez, Maria CeciliaConti, GabrielaLeone, MelisaCaro, Maria Del PilarMontecchia, Juan FranciscoZavallo, DiegoAsurmendi, SebastianCoat PoteinsTobamovirusPlant VirusesTobacco Mosaic TobamovirusSalicylic AcidsImmune ResponseProteínas de la CubiertaVirus de las PlantasTobamovirus del Mosaico del TabacoÁcido SalicílicoRespuesta InmunológicaSystemic MovementDELLA ProteinsMovimiento SistémicoProteínas DELLAAn important aspect of plant–virus interaction is the way viruses dynamically move over long distances and how plant immunity modulates viral systemic movement. Salicylic acid (SA), a well-characterized hormone responsible for immune responses against virus, is activated through different transcription factors including TGA and WRKY. In tobamoviruses, evidence suggests that capsid protein (CP) is required for long-distance movement, although its precise role has not been fully characterized yet. Previously, we showed that the CP of Tobacco Mosaic Virus (TMV)-Cg negatively modulates the SA-mediated defense. In this study, we analyzed the impact of SA-defense mechanism on the long-distance transport of a truncated version of TMV (TMV ∆CP virus) that cannot move to systemic tissues. The study showed that the negative modulation of NPR1 and TGA10 factors allows the long-distance transport of TMV ∆CP virus. Moreover, we observed that the stabilization of DELLA proteins promotes TMV ∆CP systemic movement. We also characterized a group of genes, part of a network modulated by CP, involved in TMV ∆CP long-distance transport. Altogether, our results indicate that CP-mediated downregulation of SA signaling pathway is required for the virus systemic movement, and this role of CP may be linked to its ability to stabilize DELLA proteins.Instituto de BiotecnologíaFil: Venturuzzi, Andrea Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Venturuzzi, Andrea Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Rodriguez, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Rodriguez, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Conti, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Leone, Melisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Leone, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Caro, María del Pilar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Caro, María del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Montecchia, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Montecchia, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); ArgentinaFil: Zavallo, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2024-08-05T10:10:50Z2024-08-05T10:10:50Z2021-05info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/18792https://onlinelibrary.wiley.com/doi/10.1111/tpj.152681365-313Xhttps://doi.org/10.1111/tpj.15268The Plant Journal 106 (4) : 896–912 (May 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrialinfo:eu-repograntAgreement/INTA/2019-PD-E4-I085-001, Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógenoinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:31:46Zoai:localhost:20.500.12123/18792instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:31:47.293INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Negative modulation of SA signaling components by the capsid protein of tobacco mosaic virus is required for viral long-distance movement
title Negative modulation of SA signaling components by the capsid protein of tobacco mosaic virus is required for viral long-distance movement
spellingShingle Negative modulation of SA signaling components by the capsid protein of tobacco mosaic virus is required for viral long-distance movement
Venturuzzi, Andrea Laura
Coat Poteins
Tobamovirus
Plant Viruses
Tobacco Mosaic Tobamovirus
Salicylic Acids
Immune Response
Proteínas de la Cubierta
Virus de las Plantas
Tobamovirus del Mosaico del Tabaco
Ácido Salicílico
Respuesta Inmunológica
Systemic Movement
DELLA Proteins
Movimiento Sistémico
Proteínas DELLA
title_short Negative modulation of SA signaling components by the capsid protein of tobacco mosaic virus is required for viral long-distance movement
title_full Negative modulation of SA signaling components by the capsid protein of tobacco mosaic virus is required for viral long-distance movement
title_fullStr Negative modulation of SA signaling components by the capsid protein of tobacco mosaic virus is required for viral long-distance movement
title_full_unstemmed Negative modulation of SA signaling components by the capsid protein of tobacco mosaic virus is required for viral long-distance movement
title_sort Negative modulation of SA signaling components by the capsid protein of tobacco mosaic virus is required for viral long-distance movement
dc.creator.none.fl_str_mv Venturuzzi, Andrea Laura
Rodriguez, Maria Cecilia
Conti, Gabriela
Leone, Melisa
Caro, Maria Del Pilar
Montecchia, Juan Francisco
Zavallo, Diego
Asurmendi, Sebastian
author Venturuzzi, Andrea Laura
author_facet Venturuzzi, Andrea Laura
Rodriguez, Maria Cecilia
Conti, Gabriela
Leone, Melisa
Caro, Maria Del Pilar
Montecchia, Juan Francisco
Zavallo, Diego
Asurmendi, Sebastian
author_role author
author2 Rodriguez, Maria Cecilia
Conti, Gabriela
Leone, Melisa
Caro, Maria Del Pilar
Montecchia, Juan Francisco
Zavallo, Diego
Asurmendi, Sebastian
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Coat Poteins
Tobamovirus
Plant Viruses
Tobacco Mosaic Tobamovirus
Salicylic Acids
Immune Response
Proteínas de la Cubierta
Virus de las Plantas
Tobamovirus del Mosaico del Tabaco
Ácido Salicílico
Respuesta Inmunológica
Systemic Movement
DELLA Proteins
Movimiento Sistémico
Proteínas DELLA
topic Coat Poteins
Tobamovirus
Plant Viruses
Tobacco Mosaic Tobamovirus
Salicylic Acids
Immune Response
Proteínas de la Cubierta
Virus de las Plantas
Tobamovirus del Mosaico del Tabaco
Ácido Salicílico
Respuesta Inmunológica
Systemic Movement
DELLA Proteins
Movimiento Sistémico
Proteínas DELLA
dc.description.none.fl_txt_mv An important aspect of plant–virus interaction is the way viruses dynamically move over long distances and how plant immunity modulates viral systemic movement. Salicylic acid (SA), a well-characterized hormone responsible for immune responses against virus, is activated through different transcription factors including TGA and WRKY. In tobamoviruses, evidence suggests that capsid protein (CP) is required for long-distance movement, although its precise role has not been fully characterized yet. Previously, we showed that the CP of Tobacco Mosaic Virus (TMV)-Cg negatively modulates the SA-mediated defense. In this study, we analyzed the impact of SA-defense mechanism on the long-distance transport of a truncated version of TMV (TMV ∆CP virus) that cannot move to systemic tissues. The study showed that the negative modulation of NPR1 and TGA10 factors allows the long-distance transport of TMV ∆CP virus. Moreover, we observed that the stabilization of DELLA proteins promotes TMV ∆CP systemic movement. We also characterized a group of genes, part of a network modulated by CP, involved in TMV ∆CP long-distance transport. Altogether, our results indicate that CP-mediated downregulation of SA signaling pathway is required for the virus systemic movement, and this role of CP may be linked to its ability to stabilize DELLA proteins.
Instituto de Biotecnología
Fil: Venturuzzi, Andrea Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Venturuzzi, Andrea Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Rodriguez, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Rodriguez, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Conti, Gabriela. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Conti, Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Leone, Melisa. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Leone, Melisa. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caro, María del Pilar. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Caro, María del Pilar. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Montecchia, Juan Francisco. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Montecchia, Juan Francisco. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zavallo, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular (IABIMO); Argentina
Fil: Zavallo, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Asurmendi, Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Asurmendi, Sebastian. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description An important aspect of plant–virus interaction is the way viruses dynamically move over long distances and how plant immunity modulates viral systemic movement. Salicylic acid (SA), a well-characterized hormone responsible for immune responses against virus, is activated through different transcription factors including TGA and WRKY. In tobamoviruses, evidence suggests that capsid protein (CP) is required for long-distance movement, although its precise role has not been fully characterized yet. Previously, we showed that the CP of Tobacco Mosaic Virus (TMV)-Cg negatively modulates the SA-mediated defense. In this study, we analyzed the impact of SA-defense mechanism on the long-distance transport of a truncated version of TMV (TMV ∆CP virus) that cannot move to systemic tissues. The study showed that the negative modulation of NPR1 and TGA10 factors allows the long-distance transport of TMV ∆CP virus. Moreover, we observed that the stabilization of DELLA proteins promotes TMV ∆CP systemic movement. We also characterized a group of genes, part of a network modulated by CP, involved in TMV ∆CP long-distance transport. Altogether, our results indicate that CP-mediated downregulation of SA signaling pathway is required for the virus systemic movement, and this role of CP may be linked to its ability to stabilize DELLA proteins.
publishDate 2021
dc.date.none.fl_str_mv 2021-05
2024-08-05T10:10:50Z
2024-08-05T10:10:50Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/18792
https://onlinelibrary.wiley.com/doi/10.1111/tpj.15268
1365-313X
https://doi.org/10.1111/tpj.15268
url http://hdl.handle.net/20.500.12123/18792
https://onlinelibrary.wiley.com/doi/10.1111/tpj.15268
https://doi.org/10.1111/tpj.15268
identifier_str_mv 1365-313X
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E6-I116-001, Identificación y análisis funcional de genes o redes génicas de interés biotecnológico con fin agropecuario, forestal, agroalimentario y/o agroindustrial
info:eu-repograntAgreement/INTA/2019-PD-E4-I085-001, Determinación de los mecanismos de resistencia a enfermedades mediante la caracterización de las interacciones moleculares en sistemas planta-patógeno
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv The Plant Journal 106 (4) : 896–912 (May 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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