Bioactivity of Essential Oils from Patagonian Wild Plants: Acaricidal and Insecticidal Effects on Varroa destructor and Apis mellifera

Autores
Manzo, Rosa M.; Iglesias, Azucena E.; Guajardo, Jimena Johana; Amaturi, Carolina A.; Freeman, Brenda D.; López de Armentia, Juliana; Rizzuto, Susana; Maggi, Matías D.
Año de publicación
2025
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Varroa destructor represents a major threat to honeybee colonies worldwide, prompting the search for alternative organic acaricides. This study evaluated the biological activity of essential oils extracted from three Patagonian wild plants—Adesmia boronioides, Dysphania multifida, and Senecio filaginoides—on both V. destructor and Apis mellifera. Chemical analysis revealed that A. boronioides oil was dominated by esquelenone (34.49%), D. multifida by ascaridole (34.87%), and S. filaginoides by α-pinene (40.87%). All essential oils exhibited acaricidal activity, with D. multifida showing the lowest LC50 against V. destructor (1.1 µL/mL at 24 h). Toxicity assays on adult bees indicated that A. boronioides and D. multifida significantly reduced bee survival, whereas S. filaginoides did not appear to cause significant mortality (LC50 = 139.5 µL/mL). Repellency tests for A. boronioides and D. multifida showed significant mite repellence. Larval assays revealed a high survival rate under S. filaginoides treatment (survival rate > 80.24%), in contrast to the reduced viability observed with the other oils. The high selectivity index of S. filaginoides underscores its potential as a selective and safe botanical acaricide. Moreover, its LC50 decreased over time, suggesting a residual acaricidal effect. These findings support S. filaginoides as a promising candidate for sustainable V. destructor control.
EEA Esquel
Fil: Manzo, Rosa M. Consejo Nacional de Investigaciones Científicas y Técnica; Argentina
Fil: Manzo, Rosa M. Universidad Nacional de la Patagonia San Juan Bosco. Laboratorio de Investigaciones en Evolución y Biodiversidad; Argentina
Fil: Iglesias, Azucena E. Consejo Nacional de Investigaciones Científicas y Técnica; Argentina
Fil: Iglesias, Azucena E. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Centro de Investigación en Abejas Sociales (IIPROSAM); Argentina
Fil: Guajardo, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agroforestal Esquel; Argentina
Fil: Amaturi, Carolina A. Consejo Nacional de Investigaciones Científicas y Técnica; Argentina
Fil: Amaturi, Carolina A. Universidad Nacional de la Patagonia San Juan Bosco. Laboratorio de Investigaciones en Evolución y Biodiversidad; Argentina
Fil: Freeman, Brenda D. Consejo Nacional de Investigaciones Científicas y Técnica; Argentina
Fil: Freeman, Brenda D. Secretaria de Chubut; Argentina
Fil: López de Armentia, Juliana. Universidad Nacional de la Patagonia San Juan Bosco. Instituto de Biotecnología Esquel; Argentina
Fil: Rizzuto, Susana. Universidad Nacional de la Patagonia San Juan Bosco. Laboratorio de Investigaciones en Evolución y Biodiversidad; Argentina
Fil: Maggi, Matías D. Consejo Nacional de Investigaciones Científicas y Técnica; Argentina
Fil: Maggi, Matías D. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Centro de Investigación en Abejas Sociales (IIPROSAM); Argentina
Fuente
Plants 14 (16) : 2484. (August 2025)
Materia
Aceites Esenciales
Compuestos Bioactivos
Abeja Melífera
Apicultura
Control Biológico
Plantas Silvestres
Essential Oils
Bioactive Compounds
Honey Bees
Apis mellifera
Varroa destructor
Apiculture
Biological Control
Wild Plants
Región Patagónica
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/24217
Acceder
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oai_identifier_str oai:localhost:20.500.12123/24217
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Bioactivity of Essential Oils from Patagonian Wild Plants: Acaricidal and Insecticidal Effects on Varroa destructor and Apis melliferaManzo, Rosa M.Iglesias, Azucena E.Guajardo, Jimena JohanaAmaturi, Carolina A.Freeman, Brenda D.López de Armentia, JulianaRizzuto, SusanaMaggi, Matías D.Aceites EsencialesCompuestos BioactivosAbeja MelíferaApiculturaControl BiológicoPlantas SilvestresEssential OilsBioactive CompoundsHoney BeesApis melliferaVarroa destructorApicultureBiological ControlWild PlantsRegión PatagónicaVarroa destructor represents a major threat to honeybee colonies worldwide, prompting the search for alternative organic acaricides. This study evaluated the biological activity of essential oils extracted from three Patagonian wild plants—Adesmia boronioides, Dysphania multifida, and Senecio filaginoides—on both V. destructor and Apis mellifera. Chemical analysis revealed that A. boronioides oil was dominated by esquelenone (34.49%), D. multifida by ascaridole (34.87%), and S. filaginoides by α-pinene (40.87%). All essential oils exhibited acaricidal activity, with D. multifida showing the lowest LC50 against V. destructor (1.1 µL/mL at 24 h). Toxicity assays on adult bees indicated that A. boronioides and D. multifida significantly reduced bee survival, whereas S. filaginoides did not appear to cause significant mortality (LC50 = 139.5 µL/mL). Repellency tests for A. boronioides and D. multifida showed significant mite repellence. Larval assays revealed a high survival rate under S. filaginoides treatment (survival rate > 80.24%), in contrast to the reduced viability observed with the other oils. The high selectivity index of S. filaginoides underscores its potential as a selective and safe botanical acaricide. Moreover, its LC50 decreased over time, suggesting a residual acaricidal effect. These findings support S. filaginoides as a promising candidate for sustainable V. destructor control.EEA EsquelFil: Manzo, Rosa M. Consejo Nacional de Investigaciones Científicas y Técnica; ArgentinaFil: Manzo, Rosa M. Universidad Nacional de la Patagonia San Juan Bosco. Laboratorio de Investigaciones en Evolución y Biodiversidad; ArgentinaFil: Iglesias, Azucena E. Consejo Nacional de Investigaciones Científicas y Técnica; ArgentinaFil: Iglesias, Azucena E. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Centro de Investigación en Abejas Sociales (IIPROSAM); ArgentinaFil: Guajardo, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agroforestal Esquel; ArgentinaFil: Amaturi, Carolina A. Consejo Nacional de Investigaciones Científicas y Técnica; ArgentinaFil: Amaturi, Carolina A. Universidad Nacional de la Patagonia San Juan Bosco. Laboratorio de Investigaciones en Evolución y Biodiversidad; ArgentinaFil: Freeman, Brenda D. Consejo Nacional de Investigaciones Científicas y Técnica; ArgentinaFil: Freeman, Brenda D. Secretaria de Chubut; ArgentinaFil: López de Armentia, Juliana. Universidad Nacional de la Patagonia San Juan Bosco. Instituto de Biotecnología Esquel; ArgentinaFil: Rizzuto, Susana. Universidad Nacional de la Patagonia San Juan Bosco. Laboratorio de Investigaciones en Evolución y Biodiversidad; ArgentinaFil: Maggi, Matías D. Consejo Nacional de Investigaciones Científicas y Técnica; ArgentinaFil: Maggi, Matías D. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Centro de Investigación en Abejas Sociales (IIPROSAM); ArgentinaMDPI2025-10-20T14:03:13Z2025-10-20T14:03:13Z2025-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/24217https://www.mdpi.com/2223-7747/14/16/24842223-7747https://doi.org/10.3390/plants14162484Plants 14 (16) : 2484. (August 2025)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:19:47Zoai:localhost:20.500.12123/24217instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:19:47.964INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Bioactivity of Essential Oils from Patagonian Wild Plants: Acaricidal and Insecticidal Effects on Varroa destructor and Apis mellifera
title Bioactivity of Essential Oils from Patagonian Wild Plants: Acaricidal and Insecticidal Effects on Varroa destructor and Apis mellifera
spellingShingle Bioactivity of Essential Oils from Patagonian Wild Plants: Acaricidal and Insecticidal Effects on Varroa destructor and Apis mellifera
Manzo, Rosa M.
Aceites Esenciales
Compuestos Bioactivos
Abeja Melífera
Apicultura
Control Biológico
Plantas Silvestres
Essential Oils
Bioactive Compounds
Honey Bees
Apis mellifera
Varroa destructor
Apiculture
Biological Control
Wild Plants
Región Patagónica
title_short Bioactivity of Essential Oils from Patagonian Wild Plants: Acaricidal and Insecticidal Effects on Varroa destructor and Apis mellifera
title_full Bioactivity of Essential Oils from Patagonian Wild Plants: Acaricidal and Insecticidal Effects on Varroa destructor and Apis mellifera
title_fullStr Bioactivity of Essential Oils from Patagonian Wild Plants: Acaricidal and Insecticidal Effects on Varroa destructor and Apis mellifera
title_full_unstemmed Bioactivity of Essential Oils from Patagonian Wild Plants: Acaricidal and Insecticidal Effects on Varroa destructor and Apis mellifera
title_sort Bioactivity of Essential Oils from Patagonian Wild Plants: Acaricidal and Insecticidal Effects on Varroa destructor and Apis mellifera
dc.creator.none.fl_str_mv Manzo, Rosa M.
Iglesias, Azucena E.
Guajardo, Jimena Johana
Amaturi, Carolina A.
Freeman, Brenda D.
López de Armentia, Juliana
Rizzuto, Susana
Maggi, Matías D.
author Manzo, Rosa M.
author_facet Manzo, Rosa M.
Iglesias, Azucena E.
Guajardo, Jimena Johana
Amaturi, Carolina A.
Freeman, Brenda D.
López de Armentia, Juliana
Rizzuto, Susana
Maggi, Matías D.
author_role author
author2 Iglesias, Azucena E.
Guajardo, Jimena Johana
Amaturi, Carolina A.
Freeman, Brenda D.
López de Armentia, Juliana
Rizzuto, Susana
Maggi, Matías D.
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Aceites Esenciales
Compuestos Bioactivos
Abeja Melífera
Apicultura
Control Biológico
Plantas Silvestres
Essential Oils
Bioactive Compounds
Honey Bees
Apis mellifera
Varroa destructor
Apiculture
Biological Control
Wild Plants
Región Patagónica
topic Aceites Esenciales
Compuestos Bioactivos
Abeja Melífera
Apicultura
Control Biológico
Plantas Silvestres
Essential Oils
Bioactive Compounds
Honey Bees
Apis mellifera
Varroa destructor
Apiculture
Biological Control
Wild Plants
Región Patagónica
dc.description.none.fl_txt_mv Varroa destructor represents a major threat to honeybee colonies worldwide, prompting the search for alternative organic acaricides. This study evaluated the biological activity of essential oils extracted from three Patagonian wild plants—Adesmia boronioides, Dysphania multifida, and Senecio filaginoides—on both V. destructor and Apis mellifera. Chemical analysis revealed that A. boronioides oil was dominated by esquelenone (34.49%), D. multifida by ascaridole (34.87%), and S. filaginoides by α-pinene (40.87%). All essential oils exhibited acaricidal activity, with D. multifida showing the lowest LC50 against V. destructor (1.1 µL/mL at 24 h). Toxicity assays on adult bees indicated that A. boronioides and D. multifida significantly reduced bee survival, whereas S. filaginoides did not appear to cause significant mortality (LC50 = 139.5 µL/mL). Repellency tests for A. boronioides and D. multifida showed significant mite repellence. Larval assays revealed a high survival rate under S. filaginoides treatment (survival rate > 80.24%), in contrast to the reduced viability observed with the other oils. The high selectivity index of S. filaginoides underscores its potential as a selective and safe botanical acaricide. Moreover, its LC50 decreased over time, suggesting a residual acaricidal effect. These findings support S. filaginoides as a promising candidate for sustainable V. destructor control.
EEA Esquel
Fil: Manzo, Rosa M. Consejo Nacional de Investigaciones Científicas y Técnica; Argentina
Fil: Manzo, Rosa M. Universidad Nacional de la Patagonia San Juan Bosco. Laboratorio de Investigaciones en Evolución y Biodiversidad; Argentina
Fil: Iglesias, Azucena E. Consejo Nacional de Investigaciones Científicas y Técnica; Argentina
Fil: Iglesias, Azucena E. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Centro de Investigación en Abejas Sociales (IIPROSAM); Argentina
Fil: Guajardo, Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agroforestal Esquel; Argentina
Fil: Amaturi, Carolina A. Consejo Nacional de Investigaciones Científicas y Técnica; Argentina
Fil: Amaturi, Carolina A. Universidad Nacional de la Patagonia San Juan Bosco. Laboratorio de Investigaciones en Evolución y Biodiversidad; Argentina
Fil: Freeman, Brenda D. Consejo Nacional de Investigaciones Científicas y Técnica; Argentina
Fil: Freeman, Brenda D. Secretaria de Chubut; Argentina
Fil: López de Armentia, Juliana. Universidad Nacional de la Patagonia San Juan Bosco. Instituto de Biotecnología Esquel; Argentina
Fil: Rizzuto, Susana. Universidad Nacional de la Patagonia San Juan Bosco. Laboratorio de Investigaciones en Evolución y Biodiversidad; Argentina
Fil: Maggi, Matías D. Consejo Nacional de Investigaciones Científicas y Técnica; Argentina
Fil: Maggi, Matías D. Universidad Nacional de Mar del Plata. Facultad de Ciencias Exactas y Naturales. Centro de Investigación en Abejas Sociales (IIPROSAM); Argentina
description Varroa destructor represents a major threat to honeybee colonies worldwide, prompting the search for alternative organic acaricides. This study evaluated the biological activity of essential oils extracted from three Patagonian wild plants—Adesmia boronioides, Dysphania multifida, and Senecio filaginoides—on both V. destructor and Apis mellifera. Chemical analysis revealed that A. boronioides oil was dominated by esquelenone (34.49%), D. multifida by ascaridole (34.87%), and S. filaginoides by α-pinene (40.87%). All essential oils exhibited acaricidal activity, with D. multifida showing the lowest LC50 against V. destructor (1.1 µL/mL at 24 h). Toxicity assays on adult bees indicated that A. boronioides and D. multifida significantly reduced bee survival, whereas S. filaginoides did not appear to cause significant mortality (LC50 = 139.5 µL/mL). Repellency tests for A. boronioides and D. multifida showed significant mite repellence. Larval assays revealed a high survival rate under S. filaginoides treatment (survival rate > 80.24%), in contrast to the reduced viability observed with the other oils. The high selectivity index of S. filaginoides underscores its potential as a selective and safe botanical acaricide. Moreover, its LC50 decreased over time, suggesting a residual acaricidal effect. These findings support S. filaginoides as a promising candidate for sustainable V. destructor control.
publishDate 2025
dc.date.none.fl_str_mv 2025-10-20T14:03:13Z
2025-10-20T14:03:13Z
2025-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/24217
https://www.mdpi.com/2223-7747/14/16/2484
2223-7747
https://doi.org/10.3390/plants14162484
url http://hdl.handle.net/20.500.12123/24217
https://www.mdpi.com/2223-7747/14/16/2484
https://doi.org/10.3390/plants14162484
identifier_str_mv 2223-7747
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Plants 14 (16) : 2484. (August 2025)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.982451

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